Hi. all
Let me add another question linked to marcophage staining.
So far we have done macrophage staining several times with F4/80, and
in most of the cases
IHC images were quite beautiful, for liver, spleen, adipose tissue and so on.
BUT, we have not succeeded in staining lung alveolar macrophages
One of my friends(pathologist) told me that F4/80 was not good for
alveolar macrophage
but he also don't know how to deal with this problem.
Does anyone know what is appropriate antibody for staining lung
alveolar macrophage?
Thanks in advance
Hiro
2012/6/20 Elizabeth Chlipala l...@premierlab.com:
Serotec has two antibodies that will work a F4/80 and CD68, both are rat
anti-mouse. We have used them in the same application that you will be using
(mouse aorta) with good success.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com
Ship to address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer
[daniela.bode...@mcri.edu.au]
Sent: Tuesday, June 19, 2012 7:30 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Macrophage stain
Hi all,
I would like to stain for macrophages in mice aorta (5um frozen
sections). Would you have suggestions on which stains to use and perhaps
share the protocol?
Thanks in advance,
Daniela Bodemer
Research Assistant
Surgical Research, Infection and Immunity
Murdoch Childrens Research Institute
The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia
T 03 9936 6676 T (03 9936 6794)
E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au
www.mcri.edu.au http://www.mcri.edu.au/
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