[Histonet] Multiplex IF staining
Hello Hist-world, Does anyone have experience doing FISH/double IF staining? My newest project is to combine a FISH probe (texas red) with anti-Staufen IF (green secondary) and anti-MBNL1 IF (secondary TBD). So I've got a red, green, and blue (DAPI counterstain) already. If I used a yellow secondary for the MBNL1, would you be able to see the difference between that and the green? I'm running out of colors here! Any help would be greatly appreciated. Thanks so much,Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fixation for FISH
Hello Histo-land, I'm going to start doing FISH in our lab, but I've never done it before. I read that the PFA fixative solution should be made fresh; however, I have some PFA that is being stored in the -80 freezer (for less than a year). Does anyone know if this would be ok to use? And actually, if it is ok, I thought about making a batch of 3% PFA and aliquoting it into smaller containers to freeze and to use at a later time. The only reason I thought about doing that is that I've heard to make it can take awhile. But this is all new to me, so any and all help would be much appreciated. I might as well add this as well: specifically, I'll be trying to optimize a protocol for Texas red-2-O-methyl-CAG probe with IF for the MBNL1 protein. I've read that combining these 2 methods can be trickyand I've never done either of them! Is there anyone out there that can give me some advice? Thanks!Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GI Pathologist Consulting
Hello all, I was wondering if anyone knows of a GI pathologist interested in doing a quick side project as a consultant for the company I work for? If so, please message me privately and I can give you the details. Thanks so much, Adrienne Anderson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decal of bone marrows
Hi Histo-land, I have a really quick question: about how long does it take to decal a bone marrow biopsy? Thanks a bunch! Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HBV Control Block
Hello all, We are in need of a HepB control block. Does anyone have any recommendations for procuring this? I actually only need a few slides, so I’m just feeling things out. Any advice would be appreciated. Thanks! Best regards, Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin Leaching
We haven’t changed our mounting medium, but I’ll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it’s very possible I didn’t make that a point. I’ll try that. Any more tips are welcome still - thank you all! Adrienne On Jun 20, 2014, at 8:11 AM, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote: Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin Leaching
Hello all, We’re having a problem with our eosin bleeding out of the sections. I’ve read that this can be caused by not dehydrating adequately after eosin, but we’re still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Adrienne Anderson
Hello! http://www.adicea.com/lrgo/vmc/azyir/ajxo.htm Adrienne Anderson vgzb ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene/Paint Thinner
I have to admit, I don't mind the smell:) But I also like the smell of gasoline, and I know both are bad for me! Thanks again to everyone who has offered feedback on this topic. Rene, I have printed out a few of the articles you've written and am anxious to research these methods more. Thanks a bunch! Best, Adrienne On Feb 7, 2013, at 10:36 AM, Rene J Buesa wrote: For sure they do not know, I have asked a few painters. As many histotechs, they have told me that the like the smell of xylene. René J. From: White, Lisa M. lisa.whi...@va.gov To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 10:23 AM Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene Substitutes
Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Thanks again, Adrienne On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote: Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene Substitutes
Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stainer for sale
Hello all, We have an automated stainer we are looking to sell. It's a Microm DS50. If interested, please email me. Thanks! Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Happy Retirement to Jim Burchette
Jim is such an amazing person and an expert in his field. He will be missed in the Immuno lab, I'm sure! Congrats, Jim! Sounds like you'll be able to enjoy some more fly fishing time!! On Jun 21, 2012, at 1:50 PM, Teri Johnson wrote: I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. Best wishes JB! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used block cabinets
Hello, I work for the Ohio Biorepository; we have about 1 1/2 million FFPE specimen in-house and are looking for better storage of our blocks. Does anyone know where I could buy reasonably priced cabinets, in large quantity (somewhere between 500-1000 cabinets)? Any and all advice would be appreciated. Thanks! Best, Adrienne Anderson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns. Thanks, Adrienne From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening in Columbus, OH
Hello Histo-land, We are a small biotech company seeking a part-time HT or HTL for our laboratory. This would be ideal for someone with a full-time job looking for additional work hours. As our work load fluctuates, you must be flexible and willing to go with the flow. Experience in embedding, microtomy, and staining is required, but most of the work will be focused on microtomy, so a high proficiency in this is important. Please send resumes to mol...@phylogenyinc.com Thanks! Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need help with an old autostainer
Hello, I know this is probably a long shot, but I'm trying to set up a stainer and don't have a user manual. It was bought at auction by my company, so who knows how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen). I've been trying to get my hands on a manual (having trouble setting up a staining program), but I don't quite know if I'm going about it the right way...or if there is one I can actually get. Does anyone know anything about this type of stainer. Please help! Thanks, Adrienne Anderson Phylogeny Inc. Columbus, OH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] Need help with an old autostainer
Thanks for all the great responses. I have found a manual and lots of advice! Thanks, Adrienne - Forwarded Message From: Adrienne Anderson rennie1...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Tue, January 4, 2011 12:19:13 PM Subject: [Histonet] Need help with an old autostainer Hello, I know this is probably a long shot, but I'm trying to set up a stainer and don't have a user manual. It was bought at auction by my company, so who knows how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen). I've been trying to get my hands on a manual (having trouble setting up a staining program), but I don't quite know if I'm going about it the right way...or if there is one I can actually get. Does anyone know anything about this type of stainer. Please help! Thanks, Adrienne Anderson Phylogeny Inc. Columbus, OH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
