[Histonet] Multiplex IF staining
Hello Hist-world, Does anyone have experience doing FISH/double IF staining? My newest project is to combine a FISH probe (texas red) with anti-Staufen IF (green secondary) and anti-MBNL1 IF (secondary TBD). So I've got a red, green, and blue (DAPI counterstain) already. If I used a yellow secondary for the MBNL1, would you be able to see the difference between that and the green? I'm running out of colors here! Any help would be greatly appreciated. Thanks so much,Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fixation for FISH
Hello Histo-land, I'm going to start doing FISH in our lab, but I've never done it before. I read that the PFA fixative solution should be made fresh; however, I have some PFA that is being stored in the -80 freezer (for less than a year). Does anyone know if this would be ok to use? And actually, if it is ok, I thought about making a batch of 3% PFA and aliquoting it into smaller containers to freeze and to use at a later time. The only reason I thought about doing that is that I've heard to make it can take awhile. But this is all new to me, so any and all help would be much appreciated. I might as well add this as well: specifically, I'll be trying to optimize a protocol for Texas red-2-O-methyl-CAG probe with IF for the MBNL1 protein. I've read that combining these 2 methods can be trickyand I've never done either of them! Is there anyone out there that can give me some advice? Thanks!Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GI Pathologist Consulting
Hello all, I was wondering if anyone knows of a GI pathologist interested in doing a quick side project as a consultant for the company I work for? If so, please message me privately and I can give you the details. Thanks so much, Adrienne Anderson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
