[Histonet] Cytochrome Oxidase Enzyme Histochemical Staining
Anyone out there doing COX enzyme histochemistry on fresh flash frozen EQUINE muscle...I have tried several methods and fibers are not differentiating the way they should be and are very faint ..any suggestions welcome!! Thanks - Amy Amy S. Porter, HT (ASCP) Michigan State University - Department of Physiology Investigative HistoPathology Lab - Supervisor Research Core Support Facility 567 Wilson Road - Room 2201 East Lansing, MI 48824-6458 517-884-5026 port...@msu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NK Cell markers for Mouse in Paraffin
Hi all - anyone out there have an antibody that they like for NK Cells in a mouse model - I am trying to work with a mouse monoclonal (MOM) with polymer technology. Looking for assistance from labs that have established protocols. We have tried enzymatic and heat retrieval ..not getting good results. Any comments would be appreciated - willing to change antibody vendors currently using clone PK136. Thanks - Amy Amy S. Porter, HT (ASCP) Michigan State University - Department of Physiology Investigative HistoPathology Lab - Supervisor Research Core Support Facility 567 Wilson Road - Room 2201 East Lansing, MI 48824-6458 517-884-5026 port...@msu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] University Core Support Facilities
To all: I have been asked to perform a cost analysis and comparison of the rates we charge for services to our researchers here on campus and outside the university - I am looking for labs that would be will to share this information for both in house and outside clients - a lab that does both would be ideal. I would conduct these conversations off list once started to prevent overload of the list and keep your information confidential if needed. Thanks in advance - Amy Amy S. Porter, HT (ASCP) Michigan State University Investigative HistoPathology Lab Research Support Facility port...@msu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet