Re: [Histonet] [EXTERNAL] Epredia xylene substitute
Thank you! On Sunday, April 28, 2024 at 02:19:24 AM EDT, Gudrun Lang wrote: Hi Ann, we have used ShellSol for years in our VIPs until our supplier stopped it. That's also a kind of naphta with aliphatic hydrogencarbons. After that wie changed to the Sakura-substitute with the same protocol. For standard processing we had/have two stations with 60 min each at 40 °C. For fat processing the time is doubled. That works for our specimens well enough. Gudrun -Ursprüngliche Nachricht- Von: Ann Specian via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Freitag, 26. April 2024 02:07 An: Will Cavett Cc: Histonet Betreff: Re: [Histonet] [EXTERNAL] Epredia xylene substitute Hi Will, great to hear from you!Yes those are all the reasons why we are considering it, but we would like to start validating tissue and rather than reinvent the wheel, We are looking for Processing protocols that have already been tried and true. If you have any, if you could share them, it would be greatly appreciated. Sent from the all new AOL app for iOS On Thursday, April 25, 2024, 6:39 PM, Will Cavett wrote: I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps. Will Cavett, II -Original Message- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [EXTERNAL] Epredia xylene substitute
Hi Will, great to hear from you!Yes those are all the reasons why we are considering it, but we would like to start validating tissue and rather than reinvent the wheel, We are looking for Processing protocols that have already been tried and true. If you have any, if you could share them, it would be greatly appreciated. Sent from the all new AOL app for iOS On Thursday, April 25, 2024, 6:39 PM, Will Cavett wrote: I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps. Will Cavett, II -Original Message- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Epredia xylene substitute
We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding
Can anyone let me know if CAP, New York State or ISO have any required credentials for someone to be able to embed human tissue? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clarapath SectionStar
Is anyone familiar with this new technology? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene Substitute Recycler Validation
We just received a recycler and would like to know if anyone has performed a validation on the recycler prior to putting into use (separate from the processing validation). If you have, is it possible to share it with me? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hologic PAP Imaging
Is anyone using a xylene substitute when staining their PAPs and imaging using the Hologic Imagers? If you are, can you tell me which xylene substitute you are using (as long as it is FDA approved). Have you had any issues? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding
Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing required to perform embedding in NJ?Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Change in Histotech training/educational requirements
Can someone tell me if there has been a change in the training and education requirements in the New Jersey/New York area for Histotech’s. Were there any regulatory changes? Sent from AOL Mobile Mail Get the new AOL app: mail.mobile.aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin block storage
Does anybody have a reference as to the proper temperature to store paraffin blocks. What is the highest temp permitable. Sent from AOL Mobile Mail Get the new AOL app: mail.mobile.aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Proscia
Has anyone used Proscia for digital imaging? Comments? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing for iuds with tissue
Can someone tell me the correct way to bill an IUD that has tissue. If there is no tissue then we just bill cpt code 88300. If there is tissue should you bill 88305 or 88300 and 88305? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fuelgen stain for urine cytology
Does anyone have a staining procedure for the fuelgen stain for urine cytology that they can share Sent from AOL Mobile Mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Acid Hematoyxlin stain for Urine
Does anyone have a procedure for an acid hematoxylin stain for urine cytology? I believe Bostwick used to/does perform it.. Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Automated Embedding Center and Express processor
Is anyone familiar with the Sakura Automated Embeddingcenter or their Express processor? If so, can you let me know yourthoughts. Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology school in NJ
Does anyone know where to find the requirements to start aschool for Histotechnology in nj? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtome validation
Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] guidelines for using digital images as a primary diagnostic tool
Does anyone have any information in regard to using digitalimages as the primary diagnostic tool? Thanks, ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette Printers
I am looking to purchase a cassette printer. Does anyone have any suggestions as to which are the best and which should be avoided? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue processing validation
We are moving out Peloris tissue processors to another room, so we have to validate. I was wondering what other labs do as a validation for this type of move? Do you run tissue on every progam utilizing each processor for at least one of the protocols? Any help would be appreciated. thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lifting sections off of slides
Does anyone have a procedure where in you can lift the stained sections off of a slide and place them on another slide? Sent from AOL Mobile Mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Post fixing IHC slides in formalin
Does anyone post fix their IHC slides in formalin in an effort to try to reduce tissue loss? If so, does anyone have a protocol for this that they have used and have seen good results? If you have any other suggestions which can help to reduce tissue loss during IHC staining, I would love to hear from you. Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide oven temperature
Can someone tell me the range you set your slide drying oven at and what you base it on. thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOP for submitting slides for HQIP
I have to write an sop for submission of slides for HQIP. Does anyone have one they can share? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Price for preparing IHC slides
Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Radiation SOP for Histology
I need a radiation sop specific to Histology. Does anyone have one they can share? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ANP 23045 The performance of all instruments and equipment is verified before use.
Has anyone written a procedure for this new checklist item? If so, what procedure are you using to verify equipment/instrument performance prior to use? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat cleaning
ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat Cleaning
Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Voice Recognition Systems
I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Negative Controls in IHC
I have a question in regard to eliminating the use of negative controls when using a non-avidin-biotin detection system. Do you not feel that negative controls may still need to be run in tissues which are likely to be pigmented such as lymph node, skin and liver? We were thinking to eliminate the use of negatives for other tissues (cervical, GI, etc.) only.What is the general consensus? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding
We are having a problem with floaters in our blocks which occur during embedding. We have multiple forceps which are placed in heated wells and each cassette is embedded with a new forcep. We also wipe with a gauze, but we are still getting floaters embedded in the cassette from time to time. Does anyone do anything else to prevent this? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding
we clean them at the start of our shift, but not during embedding. do you clean them during embedding too? -Original Message- From: Norton, Sally sally.nor...@seattlechildrens.org To: 'Ann Specian' thisis...@aol.com; histonet histonet@lists.utsouthwestern.edu Sent: Fri, May 25, 2012 1:12 pm Subject: RE: [Histonet] Embedding We make sure to clean the wells also. Little flecks of tissue are almost always n there after embedding. Sally Norton eattle Children's -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Ann Specian ent: Friday, May 25, 2012 10:02 AM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Embedding e are having a problem with floaters in our blocks which occur during mbedding. We have multiple forceps which are placed in heated wells and each assette is embedded with a new forcep. We also wipe with a gauze, but we are till getting floaters embedded in the cassette from time to time. Does anyone do anything else to prevent this? hank you, Ann __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for he sole use of the intended recipient(s) and may contain confidential and rivileged information protected by law. Any unauthorized review, use, isclosure or distribution is prohibited. If you are not the intended ecipient, please contact the sender by reply e-mail and destroy all copies of he original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
