RE: [Histonet] TTF-1/background staining
Hi Paula I do not believe that RTU Abs really exist. No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab. I have used many RTU Abs which I have had to dilute to get optimal staining with Cam 5.2 a good example. On the Bond I was using it at 1:25. try titreing your Ab and see if that will reduce/eliminate your background. regards Tony Debra Siena dsi...@statlab.com 4/02/2011 8:02 am Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] lost tissue
Hi Christine You have had some good suggestions to date. Also to consider if you are using the Peloris racks with the individual spaces for cassettes I have seen this happen when cassettes are inserted into the racks. As most people load cassettes with the patient details facing up, if not careful the lid can make contact with the cassette divider and open the lid. We had this happen in my lab and now the formalin that contains the racks prior to processing is poured through a sieve before disposal. We have saved one specimen with this process and even if it is only 1 in a million it is worth it. As the tissue is not wrapped also consider that tissue will shrink during processing. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Braaten, Christine I cbraa...@cheshire-med.com 12/11/2010 1:52 am Hi everyone, Has anyone had a problem with cassettes opening during processing? Sometimes the cassettes are slightly opened at the top or bottom. We rarely lose a specimen and it's frustrating to cause the patient to be biopsied again with no explanation what happened to it. We use Surgipath micro cassettes and a Peloris processor and just in the past three or four months we have lost 2 specimens somewhere between grossing and embedding. The specimens are tiny skin biopsies and have no paper or biopsy pads in the cassette with them. They are not small enough to fit through the cassette holes. If anyone has any ideas what might be happening I would appreciate a response. I have been embedding for 8 years and it's just been in the recent past that this has happened. Thanks, Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bag Sealing system
Hi Loralee Try a butcher's supply store. They sell devices they use for vacuum packing meat. By removing the air from the bag the evaporation of formalin is almost zero and the specimens will remain intact for many years. The bags are also more hardy than those sold with basic bag sealers. This is also a cheap way of preserving specimens for teaching purposes though depending on the shape of the specimen there can be some distortion due to the vacuum process. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ McMahon, Loralee A loralee_mcma...@urmc.rochester.edu 30/09/2010 3:47 am Milestone Medical has a really neat system. www.milesonemed.com It is called the TissueSAFE. You will find it under preanalytical tools. We would love to get one for our couriers and biospecimen repository. We haven't purchased it yet. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masterson_John [masterson_j...@allergan.com] Sent: Wednesday, September 29, 2010 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John /prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt; COLOR: blue This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation. /SPAN/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HM 500 M cryostat issue
Hi Andrew I have not used that model cryostat so this may not be the answer but I have experienced this on other cryostats when the microtome has been removed for cleaning and decontamination and reinstalled with the handle in the incorrect position resulting in the counterweight being totally out of whack. When the microtome is removed the handle will naturally fall to 6 o'clock however when it is reinstalled the handle needs to be at 12 o'clock for the balance to be correct. If this is not done correctly when the handle is released the chuck will always return to the up position. I hope this helps. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Andrew Burgeson nap...@siscom.net 24/09/2010 4:07 am Hello all, I have recently been using an older model MICROM HM500 M cryostat and have been experiencing an annoying problem. When I cut a section I like to leave the top edge of the OCT anchored by not cutting through it all the way. This way I can pull tension on the bottom of the section with a paint brush and flatten it out before picking it up on the slide. The carriage that holds the chuck drifts up slightly when I cut the section, pulling the tissue and OCT up and away from the plate. I have never used a cryostat that had a carriage that drifted up in that position. Over the years, every cryostat I have used (including doing a lot of Mohs and other interoperative work)has enabled me to stop the handle at any position in which I need it to remain. Anyway, this made cutting some rather challenging sections of mouse kidney very difficult. I actually had someone stand next to me and hold the crank in place while I took the section (or else it would drift a bit). My sense is that the crank mechanism has something wrong that is causing it to not remain stationary once I take my hand off the wheel. Recently an equipment repair service came in and told me that the unit was designed that way and even that if I talked to the Germans that made it, they would say it waas designed that way. (!!) wtf? At any rate, he took out the lead conterweight and said that he would have to modify the handle balance to suit my needs by cutting off some of the weight and proceeded to get out a hack saw and started trying to saw off some lead. He got nowhere with this and stated he would have to take the unit into the shop to do this. He was also kind of insulting in that he told me that what i wanted the thing to do wasnt what it was designed to do! Any thoughts? I have asked around a bit and other histology techs tell me that they see the drifting as an abnormal occurrence and that it shouldnt do it if it's working right. I think something is off with the coupling inside or with the calibration or balance of the wheel. Any one have a comment? First time in 16 years I have had a repair person tell me I am using the machine the wrong way. This repair service is not as experienced as the one I have used for many years and I think he knows what he is doing. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently
Re: [Histonet] decontamination of cryostat
Hi Annie If you look up this article or many others like it on the net the simplest and most effective disinfectants are 70% alcohol or one of the aldehydes for most organisms remembering that you are only disinfecting a hard surface with no visible volume of liquid containing organisms. We are fortunate to have 2 Thermo cryostats which have an inbuilt decontamination system using concentrated formaldehyde which can be run overnight performing a defrost and decon and as the microtome is not in the refrigerated chamber there is no problem with ice build up in the moving parts of the microtome. American Society for Microbiology Antiseptics and Disinfectants: Activity, Action, and Resistance Gerald McDonnell1* and A. Denver Russell2 STERIS Corporation, St. Louis Operations, St. Louis, Missouri 63166,1 and Welsh School of Pharmacy, Cardiff University, Cardiff CF1 3XF, United Kingdom2 All the best Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ER clone 1D5 or SP1 ?
Hi A large number of studies have been performed which show that 6F11 will stain a number of tumours that 1D5 does not and I know a number of labs who have moved away from 1D5 because of this. Google 1D5 and 6F11 and you will find the articles. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cleaning VIP Processor containers
Hi Brandi Do you do hot water flushes? If not the film will be a build up of the buffer salts from your formalin. To flush replace your formalin containers with hot water and write a program to run each container for 10-15 minutes. This will not only clean the retorts but the fluid lines as well. This should be done weekly or at least monthly if time is an issue. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing lavages into cell pellets...
Hi Jennifer I realise there are products like Histogel about of which I have no knowledge however I have been using agar for over 30 years because I can get it easily and gratis from our microbiology department. My experience is to spin down the cells, remove the supernatent, then add fixative. The fixation helps to protect the cells from the heat of the molten agar. Heat agar until just melted to ensure minimum temperature. After adding molten agar spin again in a tube to give you a button of agar with the cells located at the pointy end. After the agar has set the agar button can be removed from the tube and stored in fixative for processing. Although there may be a small number of cells in the agar, the agar does not process well on a short cycle(ie 2 hours) Process on at least a 6 hour cycle and overnight is OK. When embedding, the cells if in sufficient quantity, are visible in the agar allowing easy orientation. All the best. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Johnson, Jennifer(Hist) jennifer.john...@genzyme.com 16/03/2010 4:38 am Hi, I am looking for a little advice from anyone who has experience processing lavage fluid into cell pellets that can be sectioned and stained. I need to work out a protocol using lavages from knockout mice that may eventually be used to look at human patients. In both models of disease I expect to collect a lot of macrophages. After reading, googling and looking up articles and the Histonet, I have come up with the following procedure. I would appreciate advice or ideas from anyone who has performed any part(s) of this type of procedure: 1. Collect lavage in PBS (perform actual lavage on euthanized mouse using PBS as the diluent). Store on ice until I get back to the lab. Question here - because I will be collecting many samples, should I spin them right away or keep them on ice and spin them all at once? Should I add a mucolytic agent (mucolexx or the like) and then store them on ice until I get back to my lab? I know that in mice, although it will be a model that has lung disease, mucus will not be a problem. However, in the human model of the disease it may be an issue. I need to see the effect of the mucolytic agent on the affected cells before using it on the patients' lavages. Does anyone out there use a specific mucolytic agent that they like? I plan on trying 2 - the Mucolexx (MSDS only lists formaldehyde) and Richard Allan Mucolytic agent (MSDS lists formaldehyde, ethylene glycol, methyl alcohol and polyethylene glycol). 2. Spin down cells at 4C for 15 minutes. Remove supernatant. Add fix - either overnight or for a few hours. Question - if I use a mucolytic agent that contains fixative - formaldehyde - do I have to rinse it out (resuspend the cells in PBS or other buffer and spin again) or can I just remove the sup and add a gel (step 4)? Is the mucolytic agent enough of a fixative (the Thermo/Richard Allan and Mucolexx mucolytic agents contain 0.3 or 3 % formaldehyde)? 3. Once the cells are pelleted, should I fix them again or just resuspend them in a gel (something like Histogel or an agar(ose)? Does anyone out there use either of these gels to look at cells? Any preferences? 4. Once the cells are in the gel, fix the cell pellet. (Is this necessary?) 5. Pray and process the pellet into paraffin (and maybe plastic too). Thanks in advance for any help you can give. Jenn Jennifer Johnson Genzyme Corp. Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 Phn - 508-271-3610 Fax - 508-872-9080 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect
Re: [Histonet] tb-controls selfmade
Hi Gudren I have not done this with TB but I have with other bacteria to produce controls for gram stains. You need to have the tissue in a nutrient broth appropriate for the organism. You need to consult with a laboratory that specialises in Mycobacteria to find the appropriate medium to use. Also what may be relevant is that in the laboratory it takes up to six weeks to culture TB. Not sure but this may be why you are not getting results. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Gudrun Lang gu.l...@gmx.at 16/03/2010 1:14 am Hi all! Can someone provide me with a procedure for selfmade Tb-control for ZN-staining. I tried incubating some lungtissue (2x2x2 cm) in a solution with 3-6 drops of bacteria-suspension for 3 hours and for 18 hours. Afterwards fixing in NBF for 4 days. I had no success. No stainable mycobacteria were found. Bye Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] alcian blue van gieson stain
Hi Margaret You perform the Alcian Blue first but as with Movatt's pentachrome you need to treat the sections after AB with alcoholic ammonia for an hour to convert the AB to Monastral Blue as the AB is soluable in picric acid and the Monastral blue resistant. Let me know if you need a method regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Perry, Margaret margaret.pe...@sdstate.edu 13/03/2010 12:30 am We want to try the double stain alcian blue Van gieson. Do you stain for the alcian blue first and then the Van gieson? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Double labeling with antibodies that need differentfixatives
Hi Phebe The first place to look when working up Antibodies is the Specification sheet from the manufacturer. If you look at the BD sheet for their CD31 Immunohistochemistry is recommended for Zinc fixed paraffin sections and acetone fixed frozeb sections but not recommended for formalin fixed paraffin sections. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au 23/02/2010 2:09 pm Hi Adam, Thanks a lot, might just give it a go on just formalin fixed tissue first. Phebe From: Adam . [mailto:anonwu...@gmail.com] Sent: Tuesday, 23 February 2010 12:01 PM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives I have no idea if it would work with regular formalin. In my experience, many antigens work as well in zinc buffered formalin without any antigen retrieval as regular formalin with antigen retrieval. But really, you just have to try it yourself. On antibodies I've gotten this to work, I use commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon, Feb 22, 2010 at 9:45 PM, Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au wrote: Hello Adam, Thank you very much for this very useful information! Do you know whether this would also work on tissue fixed with formalin instead of zinc buffered formalin by any chance? Also, could you give me the recipe for the zinc formalin and can I use a standard tissue processor for embedding in paraffin or should I use a specific protocol manually and if so, which? Thanks! Phebe From: Adam . [mailto:anonwu...@gmail.com] Sent: Tuesday, 23 February 2010 10:52 AM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy,
RE: [Histonet] Double labeling with antibodies that need differentfixatives
Hi Phebe The first place to look when working up Antibodies is the Specification sheet from the manufacturer. If you look at the BD sheet for their CD31 Immunohistochemistry is recommended for Zinc fixed paraffin sections and acetone fixed frozeb sections but not recommended for formalin fixed paraffin sections. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au 23/02/2010 2:09 pm Hi Adam, Thanks a lot, might just give it a go on just formalin fixed tissue first. Phebe From: Adam . [mailto:anonwu...@gmail.com] Sent: Tuesday, 23 February 2010 12:01 PM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives I have no idea if it would work with regular formalin. In my experience, many antigens work as well in zinc buffered formalin without any antigen retrieval as regular formalin with antigen retrieval. But really, you just have to try it yourself. On antibodies I've gotten this to work, I use commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon, Feb 22, 2010 at 9:45 PM, Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au wrote: Hello Adam, Thank you very much for this very useful information! Do you know whether this would also work on tissue fixed with formalin instead of zinc buffered formalin by any chance? Also, could you give me the recipe for the zinc formalin and can I use a standard tissue processor for embedding in paraffin or should I use a specific protocol manually and if so, which? Thanks! Phebe From: Adam . [mailto:anonwu...@gmail.com] Sent: Tuesday, 23 February 2010 10:52 AM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy,
Re: [Histonet] Polarizing filters
I worked with a pathologist many years ago who would use one piece of polarising glass on the light source and wear his sunglasses. It worked fine. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Eridana erid...@cox.net 18/09/2009 3:49 am You can use glass camera filters. At the store they had a huge supply of polarizing filters. I bought 2 for about $20 each that were the same brand, but not even the same diameter. I put one on top of the slide and one on the light source and it worked great. I also rotated the light source filter since it was too easy to bump the slide when trying to rotate the upper one. It was really interesting to see all the non collagen that was positive in the staining but not when polarized. Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2010 San Diego, CA 92071 858 534 7438 Date: Wed, 16 Sep 2009 14:12:38 -0400 From: Monfils, Paul pmonf...@lifespan.org Subject: RE: [Histonet] Polarizing filters To: histonet@lists.utsouthwestern.edu Message-ID: 4ebff65383b74d49995298c4976d1d5e03835...@lsriexch1.lsmaster.lifespan.org Content-Type: text/plain;charset=iso-8859-1 The polarizer and analyzer are identical filters, and either of them can be used in either location. One must be between the light source and the slide being viewed. The other must be between the slide being viewed and your eye or camera. I place one filter directly on top of my illuminator. The other is in a filter slide in the microscope column, which can be pushed into the light beam or pulled out of it, but you can also place it directly on top of the slide. You rotate either filter to achieve the polarization effect. I rotate the lower one since the other one is not accessible. These filters cut down the light intensity substantially, so you should use them with maximum brightness of the illuminator, iris diaphram wide open, and with neutral density or any other kinds of filters removed from the light beam, including the blue filter if you normally use one. Polarizing filters can be purchased at any camera store, and some science supply companies sell them. Get good quality glass filters though, not cheap plastic ones. -- From: histonet-boun...@lists.utsouthwestern.edu on behalf of jstaruk Sent: Thursday, September 10, 2009 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Polarizing filters Does anyone know where I can find the two appropriate filters (lenses) needed to polarize the congo red and Sirius red stains? I have an Olympus CH-2 that needs to be fitted. I understand I need a polarizer lens and an analyzer lens. Are these two different lenses or the same lens, just in different locations on the microscope? Thank you Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the
Re: [Histonet] water on slides?
If your solutions are clean the cause may be that the level of the water wash station on your stainer is higher than the level of the subsequent alcohol container which means that some water will not be removed from the top of the slide. This is common with running water washes on stainers as the water pressure will elevate the level. A common artifact appears as a blue stripe down the slide caused by the water running down and removing the eosin. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Va Paula Sicurello vapat...@yahoo.com 25/08/2009 7:30 am I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] water on slides? To: histonet@lists.utsouthwestern.edu, Tina J. Hayes tina.ha...@va.gov Date: Monday, August 24, 2009, 6:00 PM We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Hayes, Tina J. tina.ha...@va.gov 8/24/2009 12:41 PM We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an
Re: [Histonet] Pathology to OR Communication System
Hi Luke A simple cheap solution would be to buy telephones for the OR and the lab that have speaker phone capabilities. No expensive purchase cost or installation required. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Perkocha, Luke luke.perko...@ucsf.edu 23/06/2009 2:41 am Hello All, We have a very old speakerphone system that we use to call from the pathologist's office in to our operating rooms to discuss frozen section diagnoses with the surgeons. Both sides are yelling and straining to hear and we're concerned about the risk for miscommunication. We can't go into the OR directly, since Pathology and the OR are too far apart physically. We're looking for some sort of telephone-based communications system, perhaps with a speaker and microphone that can be mounted near the surgeon, so that when we call into the OR with the frozen section diagnosis, it can be switched to speaker and the call can be continued with direct and audible communication between the pathologist on the phone in his/her office and the surgical team at the head of the operating table. Does anyone out there have a system like this? Do you know of a commercial vendor who makes something that would work? We tried an expensive Polycom system meant for conference calls, but its 360 degree microphones picked up too much background noise in the OR. It seems like it should be a simple Radio Shack project, but we're all techno-challenged. Any help would be appreciated. Thanks. Luke Perkocha luke.perko...@ucsf.edumailto:luke.perko...@ucsf.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au jeff jlhow...@yrmc.org 15/04/2009 1:55 am Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: FW: [Histonet] (no subject)
As per Young's response, I have never diluted the solution further but found that if there was some drop in the level of the solution due to evaporation it could be topped up with deionised water as long as the pH was not changed. This was on the advice of the DAKO rep at the time. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Young Kwun kw...@email.cs.nsw.gov.au 15/04/2009 1:29 pm I also used Dako's Target Retrieval buffer in the past and it was also possible to dilute further up to 1:20 or 1:40 (instead of 1:10) with good results. Young -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Wednesday, 15 April 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] (no subject) I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au jeff jlhow...@yrmc.org 15/04/2009 1:55 am Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http
Re: [Histonet] Strange circles in IHC slides
Hi Looking at the images this appears to be the prozone effect which is caused by the primary antibody concentration being too high and has been well reported in both immunohistochemistry, immunology and serology articles. Do a google serch for prozone phenomenon and you will find an explanation of the theory involved. The classic feature are the circular areas which are unstained and this is reinforced in the images by the amount of background staining in the areas that are stained. Try titrating your primary out and I am sure will eliminate the problem. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au V. Neubert histonet.nos...@vneubert.com 3/04/2009 2:12 am Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Calcium salts
Hello I am trying to differentiate Calcium Phosphate from Calcium Carbonate which both stain with the von Kossa method. Is there a pretreatment for which only one is soluble that could be used to differentiate the 2 components. thanks Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] overseas jobs
Hello Joe This is an interesting question following on from all of the discussion recently about uncertified techs. In Australia you need tertiary qualifications to work in a technical position in Histology. Histologists are regarded and paid at the same level as all other staff working in medical pathology laboratories. There are 2 levels of staff with those people with an Associate Diploma called Laboratory Technicians and those with a degree called Medical Scientists ( a little extra pay) generally, though the names can vary a little from region to region. Most Scientists have done a B.Sc in Medical Laboratory Science which involves studying Histology, Haematology, Biochemistry, Blood Transfusion Medicine, Microbiology, Immunology and Molecular Studies and qualifies the graduate to work in any of these fields and I have known a number of people including my wife who have changed vocation more than once in their working life. She started in Microbiology had 8 years in Histology including EM moved to Haematology for a few years and now runs a smaller multiskilled laboratory where she does Haematology, Blood Banking, Biochemistry and limited Microbiology on a daily basis. The first 7 years of my working life were spent in Histology during the day and on the on-call roster for nights and weekends to do Haematology, Blood Banking, Biochemistry and Microbiology. Hence the equal status with other disciplines. To get back to your original question she would need firstly to get a work visa as in my organisation (public government laboratory) we are not permitted to interview anybody who does not have a work visa and we can not arrange for people to get them. Secondly she would need to get her qualifications assessed by either a government body or our institute the Australian Institute of Medical Scientists (AIMS). Their website can be found on the net. If she is graded at degree level she can be employed as a Medical Scientist, if assessed at Diploma level employed as a Technician but if it is assessed below that level such as a certificate she could only be employed as a Laboratory Assistant. ( much less pay) I hope this is useful. P.S. I always enjoy your insightful contributions to the Histonet. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au JoeNocito jnoc...@satx.rr.com 27/02/2009 9:41 am Howdy Histo land, I'm posting this message for one of my current students. She graduates in August from the Histology program and wants to know what the job market is overseas, especially in the U.K. Any ideas? What will she have to do to be able to work in Europe or Australia? Thanks for your time. JTT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
Re: [Histonet] equation problem PLEASE help
Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.1064gor 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0 = 94 (93.984962 exactly) 0.01064 Therefore you can add 1ml stock to 93ml of diluent to get your desired concentration.. I hope this is useful regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au e...@georgetown.edu 8/01/2009 7:46 am Good afternoon, I am hoping someone out there will take pity on a mathematically challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] equation problem PLEASE help
Hello Eva Unfortunately some of the script did not transfer in the original mail and 10-9 should read 10 to the minus 9 as the superscript was lost. The same for 10-8. Also there should have been a division line between 1.0 and 0.01064 in the calculation. I hope this helps. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Anthony Reilly tony_rei...@health.qld.gov.au 8/01/2009 12:39 pm Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.1064gor 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0 = 94 (93.984962 exactly) 0.01064 Therefore you can add 1ml stock to 93ml of diluent to get your desired concentration.. I hope this is useful regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au e...@georgetown.edu 8/01/2009 7:46 am Good afternoon, I am hoping someone out there will take pity on a mathematically challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide drying
Hi Erin While the request from your pathologists seems unreasonable from my experience the following steps will give the best results. Use a rapid drying mountant many of which are on the market. Consult your local suppliers. Dry in an incubator as you have been doing but when you remove from the incubator allow the slides to cool before filing or even sorting into piles. The mountant may be dry but the heat will make it sticky gluing the slides together. Just think of new bitumen on a very hot day. All the best. Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Martin, Erin erin.mar...@ucsf.edu 23/12/2008 5:06 am Hello everyone, I was asked to find out how to dry slides quickly. They are glass coverslipped in an automated coverslipper at the reference lab we use and the our docs want them filed in less than 12 hours from the time they are coverslipped. We have been putting them in a 125 degree C convection oven for a few hours but the slides still get all stuck together in the file. They will not consider film coverslipping. Does anyone else file this quickly? I am grateful for any suggestions! Erin Martin UCSF Dermatopathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
