Re: [Histonet] Teaching color blind Histo student

[B kB via Histonet]

Hi,

Colourblindness ain’t a problem at all.
I know, because I already work for 35 years in the histopathogy.
Just a few things that causes problems for me:
Grossing: I describe tissue in the way I see, mostly I call something with
2 colours, p.e. “ the serose is brownish grey” (while collegaes mostly
mention 1 colour. But I can see the difference between normal and abnormal
tissue, that’s more important.
Embedding: only skin biopsies is it difficult to oriëntate when the colour
of the dermis is the same as the subcutis (in my eyes  ;-). So with that, I
ask my collegaes to embed these biopsies for me.
Staining: When I have to do the stainings, I just have to following the
protocol. In the SOP, There is always a example of the control slide. I
just have to compare the determination with the example of the SOP .

These are just a few example.
A colourblind technologist could be a great technician, with just a very
small restriction.

Regards,
Bert klein Brink
Chief histotechologist



Op za 27 mrt. 2021 om 02:41 schreef Ruppert, Amysue via Histonet <
histonet@lists.utsouthwestern.edu>

> Hello, we currently have a color blind Histology student.  Does anyone
> have any helpful hints to share about learning in the Histology setting
> with colorblindness?  Note,  we had previous student with colorblindness,
> many years ago, and he did fine.  But most of the teachers that helped him
> are now retired.
>
>
> Thank you
>
> amysue ruppert
>
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Re: [Histonet] Permanent mountant for Oil Red O

[B kB via Histonet]

Hi Christopher,

You van try Imsol-mount
Vendors are for example Sigmaaldrich or VWR .

After a staining, airdry the solide. Cover with mislopen-mount and let this
dry for a while ( no problem with higher temps). After that, You can cover
it with glass or tape.

Goed luck,
Bert klein brink
Gelre Hospital, Apeldoorn, Netherlands



Op ma 16 sep. 2019 om 06:04 schreef Hagon, Christopher (Health) via
Histonet 

> UNCLASSIFIED
>
> Hello Histonetters,
>
> We do Oil Red O stains on frozen section post mortem tissue, and need to
> find a permanent aqueous mounting medium. We used to use the Thermo-Fisher
> Perm-mount, but can't seem to get it anymore. As they are looking for fat
> deposits, we can't use any solvent based solutions, and the only aqueous
> ones we've found aren't permanent. We have to send the slides off site
> after staining, so the chances of the coverslip moving in transport is
> fairly high.
>
> Does anyone else have this issue and what did you end up using?
>
> Thanks in advance,
>
> Chris Hagon | Senior Scientist, Anatomical Pathology
> ACT Pathology | health.act.gov.au
> Phone (02) 5124 2874
>
>
>
>
>
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[Histonet] Problems with Ultraview Amplification Kit

[B kB via Histonet]

Hi,

A question aspecially for the the users of the Roche Benchmark Ultra.
Recently we are having troubles with the ultraview amplification kit for
the ultra.
IT causes a lot of unwanted background.
We tested a lot, to reduce this problem, but the problem didn’t disappear.
The service center in Europe (Mannheim Germany) couldn’t find the course
and send us a new kit with different lotnumber. This wasn’t the correct
action Roche should do. Most of the users of the Ultra in the Netherlands
have contact with eachother and we knew that alle that labs had the same
problems with this kit. The new lotnumber they send us was already tested
in another lab and gave also that background. Roche was known by this. A
strange action to send a “ new” product which was also inadequate. it looks
Roche is having problems managing a good ampliciation Kit.
Do you (users of the benchmark ultra using the ultraview amplication kit)
recognize this ?
Do You have problems with specific lotnumbers?

Regards,
Bert klein Brink
Head Department Histopathology
Gelre Hospital Apeldoorn, Netherland
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Re: [Histonet] Disposal of blocks and slides

[B kB via Histonet]

Hello,

in the Netherlands (Europe), the Dutch Society of Pathologists, has made a
document called "code for good use of body materials".
This advice is established after an juridical consultation in connection
with the excisting legal provisions.

The standard for now (since 2011) is:

blocks   115 years
frozen material   30 years
slides (glass or digital) 30 years
reports   30 years

Bert klein Brink
Head of Department Histopathology
Gelre Hospital
Apeldoorn (Netherlands)
Op 12 jan. 2016 14:34 schreef "Charles Riley via Histonet" <
histonet@lists.utsouthwestern.edu>:

> Hello all,
>
>  What are the guidelines for disposal of blocks and slides? This was never
> discussed in my program and I am now in charge of the department. No one
> who currently works here has been through the process. Any help will
> greatly be appreciated.
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Doctors Pathology Services, Dover DE
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