RE: [Histonet] On the lighter side...
41 years also!! Sharon Beckham Head, Histotechnology Stowers Institute for Medical Research Kansas City, MO -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, August 07, 2014 2:10 PM To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sequenza units for manual immunohistochemistry
I have used them for quite some time. I was having problems with uneven staining and determined that it was from the being too full and was drawing up liquid from the bottom. There is a small opening on the end and I just empty the solution from it. I usually empty it after rinsing the antibody off and then usually before putting DAB or DAPI on depending on the number of slides stained. As for washing, I just wash in a solution of soapy water and then rinse in distilled. I use the coverplates for several staining times. I love the sequenza and prefer it to having an automatic stainer. Sharon Beckham, HT (ASCP) Head, Histotechnology Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 (816)926-4305 s...@stowers.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Wednesday, June 06, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sequenza units for manual immunohistochemistry Hello Histonet, I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent waste in the bottom from all your additions and washes. Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom. Has anyone had any issues using these units? How do people prevent a large volume from building up in the bottom? How are people cleaning their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | Téléphone: 403-382-5500 ext 5613/5511 Facsimile | Télécopieur: 403-382-5585 Government of Canada | Gouvernement du Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Let's talk forceps
Bob, I am so glad that someone else feels the same way as I do. I'm not the only person of a certain age in this histo world. Everytime I hear the word forceps, I think about giving birth. Both my kids were pulled out with forceps back in the '70's. I HATE that word! In histology I use tweezers! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Friday, April 06, 2012 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Let's talk forceps Arrrgh! Tweezers. Pickups. Grumpy old doc notes that in his day (1960s) the word forceps meant obstetrical forceps and nothing else. I was taught how to apply forceps, though medical students weren't actually allowed to. My eldest daughter was delivered by the Professor of Forceps (a much beloved practicing obstetrician with ideas far ahead of his time) to a crowd of admiring medical students, with an axis traction bar forceps (don't ask!) in the greatest feat of obstetrical grandstanding I ever witnessed. I'm quite sure that nobody misses obstetrical forceps! The VBAC came into use not long after I graduated. Thanks for the tip about the ergonomic, uh, tweezers. Filed for future reference. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: Redneck Lent
Hey how bout us rednecks? This redneck wasn't at all offended. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate. Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 From: Davide Costanzo [pathloc...@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M dkb...@chs.netmailto:dkb...@chs.net wrote: For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050tel:804-765-5050 l FAX 804-765-8852tel:804-765-8852 From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfra...@hotmail.commailto:jfra...@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeri...@yahoo.commailto:spoeri...@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.green...@hobbylobby.commailto:karen.green...@hobbylobby.com; stewartdap...@hotmail.commailto:stewartdap...@hotmail.com; yvette.fette...@basf.commailto:yvette.fette...@basf.com; footch...@yahoo.commailto:footch...@yahoo.com; frazeeli...@hotmail.commailto:frazeeli...@hotmail.com; jfra...@hotmail.commailto:jfra...@hotmail.com; donna.lu...@gmail.commailto:donna.lu...@gmail.com Kerri - Forwarded Message - From: Sharen Pray praysha...@yahoo.commailto:praysha...@yahoo.com To: Ruth Posey ruthalpo...@yahoo.commailto:ruthalpo...@yahoo.com; LueAnn Root lar...@ymail.commailto:lar...@ymail.com; Marjorie Norris nursenor...@yahoo.commailto:nursenor...@yahoo.com; Tom Voss, Sr. tomv...@wildblue.netmailto:tomv...@wildblue.net; Taber Stewart texcon.ta...@gmail.commailto:texcon.ta...@gmail.com; MONTIE L WINTERS molo...@yahoo.commailto:molo...@yahoo.com; Terry Maloney maloney_te...@yahoo.commailto:maloney_te...@yahoo.com; kerri spoering spoeri...@yahoo.commailto:spoeri...@yahoo.com; Kenny Debbie Hager kanddha...@att.netmailto:kanddha...@att.net Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, You were born a Baptist, and raised a Baptist, but now you are a Catholic. Bubba's neighbors were greatly
RE: [Histonet] re: april fools prank
Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. Sharon -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of heather marlatt Sent: Wednesday, March 21, 2012 10:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: april fools prank I've been known to leave a fake spider in an embedder for the morning person.although it wasn't april fools just for fun it got a great reaction :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ahh...
We do that a lot and have no problem at all. Sharon Beckham Head, Histotechnology Stowers Institute for Medical Research 1000 E 50th St Kansas City, MO 64110 816-926-4305 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, November 29, 2011 10:03 AM To: Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ahh... That is what I would do. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Tuesday, November 29, 2011 10:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ahh... So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today. They are mouse samples, so smaller than others. Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and then tomorrow just put them in the embedder to remelt? Or should something else be done to preserve them. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Must be Monday...
Yes it was this past weekend back when. My alarm clock went to DST Sunday morning. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 7:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IF Doublestaining
Yes, I do double staining very frequently the way Adam stated. I hardly ever have to do the sequential staining. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, May 13, 2010 1:44 PM To: Igor Deyneko Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IF Doublestaining In my experience, most fluorophores are quite stable to many washes. Here is what I would try at first: 1) Stain with rat anti-mouse and rabbit anti-human overnight at 4C in a humidified chamber. Dilute each antibody in a single volume of your favorite staining buffer (PBS, TBS, TBS-T). For example, if your rat antibody needs to be diluted in 1:100 and your rabbit needs to be diluted 1:200, take an aliquot of 200 uL of buffer and add 2 uL of the rat primary and 1 uL of the rabbit. Add the appropriate volume (usually 50-200 uL) of that mixture to each slide. 2) Wash the next day, and add your secondaries again to a single mixture for 1 hour at room temperature. As long as your secondaries have been highly cross adsorbed to many species, you shouldn't have a problem. 3) Wash, counterstain, mount, enjoy. I really don't think you need to do sequential staining for this. I've heard anecdotal reports of two primaries or two secondaries forming immune complexes when mixed together, but I've never really had that problem. Good luck, Adam On Thu, May 13, 2010 at 1:00 PM, Igor Deyneko igor.deyn...@gmail.comwrote: Hello Everyone! I'm planning to try some IF co-staining with 2 antibodies, one is a rat-anti-mouse and the other one is rabbit anti-human on a xenograft, each has an appropriate secondary, donkey anti rat and donkey anti-rabbit, conjugated to 488 and 593. Can someone advise the best way to perform such a procedure, I'm afraid the rules of sequential staining might not work due to fluorophore instability with washes. if anyone has performed such type of stain, i would appreciate any tips. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histology Stories
Your introduction into histology brought back a very funny memory for me. My kids are in their 30's now, but when my daughter was 7 or 8 she also saw her first grossing specimen which was also a leg. She tried to talk about it in show and tell and her teacher made her stop. She didn't want to hear about it and my daughter was so excited about being able to share the information with her classmates. When my son was 14 or 15 he wanted to see a brain and we happened to have one from an autopsy case. I took it out and he got one whiff of the formalin and said Mom, no wonder you are so weird, having to smell that stuff everyday. No one can appreciate what we do quite like our children!! Jessica, that was a really cool story about your Mom introducing you to histology. It brought a tear to my eye! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Thursday, March 11, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a family of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say Happy Histologist Professional Day! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] G 20 MEETING NEWS
I disagree. This is something I didn't know about and evidently it will effect our profession. No one enjoys getting political more than I do, but I don't consider this as being political talk. I do consider it being newsworthy and something I want to become informed about. Thanks for the heads up as I will want to follow up on this one. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 01, 2009 9:11 AM To: histonet@lists.utsouthwestern.edu; Bernie Taupin Subject: Re: [Histonet] G 20 MEETING NEWS Yes please. There are plenty of blogs out there for this kind of thing. Bernie Taupin bernietau...@ymail.com 4/1/2009 9:53 AM Please refrain from discussing politics on this list. Edwards, R.E. wrote: Apparently Obama and Brown, with the reluctant compliance of Germany and China are aiming to rationalise the pay and conditions of laboratory workers worldwide, and hopefully if they succeed the histotechs in the U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] anti-GFP
I use the Rockland goat GFP #600-101-215 and it works great. I use the GFP at 1:1000, antigen retrieval in citrate buffer, Biocare's goat HRP-polymer kit. The data sheet says to incubate for 10-15 minutes in both the probe and the polymer, but I usually do it for 7 minutes. Cuts back some on background and overstaining. For fluorescence I use the anti-goat Alexa 488. I found the Rockland GFP to be much more reliable than the Novus. Sharon -Original Message- From: Johnson, Teri Sent: Tuesday, November 18, 2008 11:25 AM To: 'Gayle Callis'; MaryAnn Dixon; histonet@lists.utsouthwestern.edu Cc: Beckham, Sharon Subject: RE: [Histonet] anti-GFP Thanks for the nod Gayle. We've converted to Rockland goat anti-GFP after having some reproducibility issues with the rabbit polyclonal we were using from Novus. I agree with your recommendation to use an indirect IHC method to increase the signal. If she's using a rabbit anti-GFP/Alexa 488 she can still come back with an anti-rabbit Alexa 488 and see if that increases the signal that way. She might also try a biotinylated anti-rabbit, and come back with a Streptavidin Alexa 488. I so do not like FITC, have watched it photobleach as I was viewing it. I will leave it to my IHC Specialist to give the details of her experience with using the Rockland goat antibody. I think it works equally well using antigen retrieval or Proteinase K on formalin fixed paraffin embedded animal tissues. And she's used it both with the goat polymer kit and regular anti-goat Alexa 488. Sharon, care to elaborate on the details of your staining protocols? Teri -Original Message- From: Gayle Callis [mailto:[EMAIL PROTECTED] Sent: Tuesday, November 18, 2008 11:04 AM To: MaryAnn Dixon; histonet@lists.utsouthwestern.edu Cc: Johnson, Teri Subject: Re: [Histonet] anti-GFP The problem may be that you are using a directly conjugated antiGFP rather than a rabbit antiGFP followed by coming back with a secondary conjugated to Alea Fluor 488. This is called quenching, a phenomenom, where fluorophore moleculas too close together on cells or in tissues tend to cancel out the fluorecsing ability of the fluorophore. You should go onto internet and look at a fluorescence Jablonski diagram which show how this occurs, Olympus website also wonderful discussions in pdf form, for all fluorescence applications, including this diagram - for confocal and fluorescent microscopes. I suggest you stain for GFP (Teri Johnson method) where you retrieve, use a rabbit antiGFP, then come back with a secondary either conjugated to FITC (Jackson has excellent antibodies, or one of the Cy fluorophores, or better yet, Goat antirabbit-Alexa 488. Be sure you use Molecular Probes Prolong gold antifade mounting media after staining - this is superior for preventing fading of fluorophores, even 488. Not all aqueous mounting medias will prevent fading of fluorophores, even the Alexa dyes. Teri recommends rabbit antiGFP rather than Goat antiGFP for paraffin work, as the rabbit hosted antibody gave less background than the goat antiGFP. One can also purchase Rabbit antiGFP from Rockland. I made a CC to Teri Johnson so she is in this email loop. You may want to discuss this problem with her, and what antigen recovery method she prefers. If worse comes to worse, and you can't afford another antibody, use the antiGFP-488, come back with an antiAlexa 488 (Molecular Probes) and detect that antibody with an antibody that has FITC, or the appropriate fluorophore. A round about way, but the same type of technic used to detect FITC. Gayle M. Callis HTL(ASCP)HT,MT - Original Message - From: MaryAnn Dixon [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 18, 2008 9:19 AM Subject: [Histonet] anti-GFP Hi histonetters, I stumbled into immunofluorescence for the first time and could use some advice. I am trying to stain GFP on formalin fixed paraffin embedded sections. I have a conjugated alexa fluor 488 anti-gfp antibody from invitrogen that I've now found out was not tested on paraffin sections. I have seen articles supporting and denying that it works. In addition, do I retrieve or not as again, I've seen literature supporting both. Moreover, one article cut sections at 12 microns. My protocol for my first run consisted of a protein block for 10 minutes, blowing off, 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at room temp., buffer rinse, DI water rinse, aqueous mounting medium, and coverslip. To my best ability I performed everything in the dark. The results were that I had no fluorescing whatsoever!! Any help would be appreciated. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu
RE: [Histonet] Presidential Voting Infomation
Now, I think I've seen everything!! I can't believe you would use this venue for this trash. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Justin Thomas Sent: Monday, November 03, 2008 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to spread the wealth around, John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
