Re: [Histonet] HISTOS 5 processor
Hi Ann, How are things down there in Flodrida? Glad you get some money to buy something form Smilestone! Love, Bernie Taupin _ Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] UNSUBSCRIBE
I've heard a few other people mention it, but you know that replying UNSUBSCRIBE to the entire list doesn't do anything but send an email containing only the word UNSUBSCRIBE out to all the list-members, yeah? From: Andrea Grantham algra...@email.arizona.edu To: HISTONET histonet@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 10:21:58 AM Subject: [Histonet] UNSUBSCRIBE UNSUBSCRIBE Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FREEZY spray
I see that Kemlo is an HistoNet charlatan like the rest of us. He's the first complain about off-topic notes to the list, then goes on to send several of his own. Thank you Uncle Kemlo! :-P Way to keep this ball rolling! From: Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk To: Tony Henwood antho...@chw.edu.au; Bernie Taupin bernietau...@ymail.com; Akemi Allison-Tacha akemiat3...@yahoo.com; Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 4:05:39 AM Subject: RE: [Histonet] FREEZY spray Say thank you Uncle Kemlo!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 08 April 2009 08:58 To: Bernie Taupin; Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] FREEZY spray Hey, Would you believe that putting the word Taupin into my outlook rules actually works. Now every obnoxious post from Taupin actually disappears into my Junk folder and I do not have to read the drivel. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, 8 April 2009 3:38 PM To: Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] FREEZY spray Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. From: Akemi Allison-Tacha akemiat3...@yahoo.com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] FREEZY spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Akemi Allison-Tacha akemiat3...@yahoo.com, Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha akemiat3...@yahoo..com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] FREEZY spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections
Re: [Histonet] unsubscribe
You'd think that managing one's subscription to a mailing-list was rocket-science or something! From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Christine Jacobs jac...@mmc.org; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 9:22:38 PM Subject: RE: [Histonet] unsubscribe In order to unsubscribe please follow the directions you were provided when you signed up initially. You must actually do it yourself. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christine Jacobs Sent: Wednesday, April 08, 2009 9:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] unsubscribe Please cancel my subscription to histonet CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TMA for cell pellets?
Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! From: Thom Jensen tissuear...@hotmail.com To: louise_hart...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu Sent: Thursday, April 9, 2009 1:27:18 AM Subject: [Histonet] TMA for cell pellets? Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, Date: Thu, 2 Apr 2009 12:05:39 -0400 From: louise_hart...@urmc.rochester.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding cell pellets I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester louise_hart...@urmc.rochester.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Staying...
Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks. I, myself, have decided to stay. I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all. SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT! From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Marla Thomas mtho...@littonlab.com; histonet@lists.utsouthwestern.edu Sent: Tuesday, April 7, 2009 8:10:22 AM Subject: RE: [Histonet] Staying... Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staying... I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FREEZY spray
I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha akemiat3...@yahoo.com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] FREEZY spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the wayThis came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160° C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70° C until sectioned. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. ___ Histonet mailing list histo...@lists.utsouthwestern..edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FREEZY spray
Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. From: Akemi Allison-Tacha akemiat3...@yahoo.com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] FREEZY spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Akemi Allison-Tacha akemiat3...@yahoo.com, Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha akemiat3...@yahoo..com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] FREEZY spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160° C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70° C until sectioned.. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo..com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] onalighternote
I have just had s from Bernie Taupin and Paul Schofield, is this a record??. I, for one, am sort of bummed that R. E. Edwards never got back to us in regards to exactly what these s are. Aren't s some sort of drug , R. E.? Are you on this drug?? From: Edwards, R.E. r...@leicester.ac.uk To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nice one Kemlo
Kemlo, So do you feel like taking a moment to address why you called me out for something even when I was correct to begin with? From: Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk To: Bernie Taupin bernietau...@ymail.com; rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co..uk Sent: Monday, April 6, 2009 3:14:35 AM Subject: RE: [Histonet] Nice one Kemlo I think the benefit of getting older SHOULD be that one gets wiser. To get old without acquiring wisdom is a waste. One of those wisdoms is to know when a conversation has ended. or when there's no point due to the immaturity or lack of insight of some of the audience. You can use that Bernie, to stick at the end of your e-mails as a 'wise saying' (g). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 06 April 2009 05:28 To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sara Sorry to see you go
This is a great example of the sort of missive that could more appropriately have been sent privately, off-list, yeah? From: Akemi Allison-Tacha akemiat3...@yahoo.com To: histonet@lists.utsouthwestern.edu; SaraBreeden sbree...@nmda.nmsu.edu Sent: Monday, April 6, 2009 3:41:57 PM Subject: [Histonet] Sara Sorry to see you go Hi Sara, I for one will be sad to see your absence. Perhaps in the future, you will reconsider and join our group again. It is extremely unfortunate to see these grown men acting like FOOLS! I have been pretty much a voyeur as of late, unless I have something credible to say or ask, but I feel the urge to state my feelings as a fellow histologist. And by the way, there are a number of extremely prominent histologists and pathologists that have stated they are voyeurs too! They may not go on line, but they see your postings! They have all taken note of this stupid on-line communication. I don't think these individuals realize that the pathology community observes these communications, and this may come back to bite them in the butt later on down the line! It lowers the image of these individuals as professionals, and their names and verbiage are forever archived in our memories. Once you ring the bell, you can't un-ring it! The Histonet Forum was initially created and funded by histology vendors and concerned histologists to help us as a group. I maybe wrong, but I thought it's purpose was to expand our knowledge in this valuable profession. I have been utilizing this wonderful tool, since almost the beginning. It was an eye opening experience when I started seeing pathologists joining in. It made me realize that they too saw the merit in this wonderful tool for sharing our knowledge and experiences. I have been involved in histology since taking classes in histomicrotechnic at Tokyo University in 1968. The older I get, the more I realize how little I know. Especially with the use of molecular biology, and more and more esoteric testing. We are experiencing a metamorphosis in our field, and forums such as this, are extremely valuable! Those of us who use this tool, do so, because we care about giving the best possible patient care. At least I use to think so This is a wonderful tool for histologists, researchers, and concerned pathologists, to come together for the sole purpose to learn and grow (then there's the Friday good hearted release of steam) The Histonet is especially important for those individuals who are geographically isolated, and do not have the means to attend continuing education classes. It is my belief that the Histonet was never intended to be used as a tool as these individuals have so irresponsibly done. It is childish and unprofessional. This sort of use just shows how unthinking and selfish these individuals are. I am ashamed that the pathology community sees this sort of communication between histologists. It demeans us as a whole. I don't think these men realize what fallout may occur. We may have this taken away from us because they have not used their good judgement, common sense, and restraint! I realize that in the past, I too may have spoken a little out of turn, but this thread has become an embarrassment. I have recently accepted a employment opportunity to go back to managing a histology lab, and will ask my fellow histologists for their assistance. I truly hope there will be a Histonet available when I need you! Let's hope we can learn from this experience and move on. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Mon, 4/6/09, Breeden, Sara sbree...@nmda.nmsu.edu wrote: From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] I'm outta here To: histonet@lists.utsouthwestern.edu Date: Monday, April 6, 2009, 8:43 AM After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while. This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap. If I need help, I'll hit the books. Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
Re: [Histonet] Nice one Kemlo
Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a piece of ignorant, and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to learn anything form you! From: Rene J Buesa rjbu...@yahoo.com To: Bernie Taupin bernietau...@ymail.com Sent: Monday, April 6, 2009 9:40:18 AM Subject: Re: [Histonet] Nice one Kemlo My name (René) is not androginous you piece of ignorant. It just shows that you don't know anything about names. Ask any French speaking person about my name or the one reseved for females (Renée) and they can tell you that there is no confusion possible, you ignorant. Instead of spitting your venom try to learn René J. --- On Mon, 4/6/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] Nice one Kemlo To: rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu, alan taylor aj.tay...@blueyonder.co.uk Date: Monday, April 6, 2009, 12:27 AM 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder..co.uk Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin slides for sale
Parrafin slides? Does that mean they melt when they go into xylene?? I prefer glass slides, if you ask me From: Cathy Mayton ca...@wasatchhisto.com To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 2:30:23 PM Subject: [Histonet] paraffin slides for sale Fisher Scientific slides @ 2 $2/box 20 boxes green 5 boxes yellow Premiere brand 20 boxes green 8 boxes white Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nice one Kemlo
Bernie, get a life and quit being just down rite mean. Yes, Robyn... because you telling me to get a life isn't mean at all! Do any of you practice what you preach? Or do you all just yell at other people online while continuing to do the very thing yourself that you were yelling at them about doing? From: Robyn Vazquez vazqu...@ohsu.edu To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Monday, April 6, 2009 9:04:54 AM Subject: RE: [Histonet] Nice one Kemlo Bravo Rene...I learn A LOT from you and your know it all vast knowledge, as well as all the knowledgeable people on this list. Bernie, get a life and quit being just down rite mean. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, April 04, 2009 10:02 AM To: histonet@lists.utsouthwestern.edu; alan taylor Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Lois, do you feel like typing your reply in ALL CAPS helps get your point across better? Many folks consider ALL CAPS to be the inline equivalent of yelling. So, are you a yeller? From: Cover, Lois lco...@wmhs.com To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 5:42:00 AM Subject: [Histonet] (no subject) I HAVE SUBSCRIBED TO THE HISTONET DIGEST TO GAIN VALUABLE INFORMATION FROM MY PEERS. I THINK IT WOULD BE BEST IF THOSE WHO HAVE ISSUES WITH EACH OTHER TALK OFF-LINE. I DON'T WANT TO SPEND VALUABLE TIME READING THIS ENDLESS NONSENSE! Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Entamoeba Trophozoites
It's amazing how such a simple observation can bring out the worst (and most subjective rants) in some people, eh? How many personal insults and names did you just hurl at me here, Sonny? the most ironic part is that people like you jump down my throat for my comments but don't seem to be bright enough to restrain themselves from making the exact same sort of comments. And YOU're the one calling ME a half wit! From: kemlo ke...@f2s.com To: Bernie Taupin bernietau...@ymail.com; histonet@lists.utsouthwestern.edu Sent: Saturday, April 4, 2009 9:15:02 AM Subject: RE: [Histonet] Entamoeba Trophozoites Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nice one Kemlo
1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Entamoeba Trophozoites
I am a HE and not a SHE Oh, HAHAHA! Nice one, Kemlo! Disregard my last noes re: Gender and maybe, I dunno, CHECK YOUR OWN FACTS BEFORE CHEWING SOMEONE OUT FOR THEIRS? Everyone on this list is so quick to jump down someone else's throat for telling it like it is, completely ignorant of their own breaches in tact, their own ignorances, their own off-topicness. Everyone loves pointing the finger, yet no one can tolerate it when its actually them at fault. You guys crack me up with your logic sometimes. I fear for the world that the safety of so many imporant biopsies are trusted to so many of yours' easily-agitated, ignorant hands! From: kemlo ke...@f2s.com To: Bernie Taupin bernietau...@ymail.com; histonet@lists.utsouthwestern.edu Sent: Saturday, April 4, 2009 9:15:02 AM Subject: RE: [Histonet] Entamoeba Trophozoites Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Entamoeba Trophozoites
Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC, decalcification preferences
I, personally, think EDTA SUCKS. a) it takes too long b) its not safe - http://en.wikipedia.org/wiki/EDTA#Toxicity_and_environmental_considerations c) its to old-fashioned. Me, I'm a big proponent of Formic Acid decal. Holla off-list if you want me to hit you up with a protocol, yo. From: Amy Porter port...@msu.edu To: histonet@lists.utsouthwestern.edu; Nicole Collette collet...@mail.llnl.gov Sent: Wednesday, April 1, 2009 12:56:43 PM Subject: Re: [Histonet] IHC, decalcification preferences All the mouse femurs and tibias done in our laboratory are decaled in 14% EDTA and we have a high success rate with our immunohistochemistry and Tunel staining. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Nicole Collette collet...@mail.llnl.gov To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 01, 2009 12:44 PM Subject: [Histonet] IHC, decalcification preferences Hello, all, I am going to be doing some IHC on adult mouse bone, and since I'm new to all this antigen preservation stuff, was wondering the best way to decalcify? Normally, I use high percentage EDTA (17%) and determine end point by weight loss/weight gain. (up until now have been using this protocol for LacZ stains, works like a charm! ) We are comparing several lines of mice with bone mineral density differences, and endpoint is very phenotype and age-dependent. It takes a long time, but the results are worth the wait. I also have Cal-Rite, which is a formaldehyde/formic acid decalcifier, and obviously works much faster. I am leaning toward the EDTA, since it seems to work well for the LacZ, which is sensitive to lots of other processes, but if there's a reason not to, please let me know. Thanks for all your help and support, I have so far been given great advice from this listserv. Sincerely, Nicole Collette LLNL/UCB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Off topic posts
Take it off the list and respond to one another privately. Says he on the list and not privately From: Dawson, Glen gdaw...@dynacaremilwaukee.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 8:52:28 AM Subject: RE: [Histonet] Off topic posts KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one another privately. It's fast becoming like observing a daycare here. Glen Dawson IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: The end of Histonet
It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. And you plan to remedy this by posting more prattle about the prattle about the endless prattle? From: Geoff McAuliffe mcaul...@umdnj.edu To: Bernie Taupin bernietau...@ymail.com Cc: HistoNet histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 10:10:12 AM Subject: The end of Histonet It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam It was a link to a video, I believe, not a scam. From: Geoff McAuliffe mcaul...@umdnj.edu To: Mark Ray darkd...@comcast.net Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 10:12:30 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] onalighternote
I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. From: Va Paula Sicurello vapat...@yahoo.com To: Jeanine (CDC/CCID/NCZVED)Bartlett j...@cdc.gov; R.E.Edwards r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu; Bernie Taupin bernietau...@ymail.com Sent: Tuesday, March 31, 2009 7:01:14 PM Subject: Re: [Histonet] onalighternote Hey, we can't help it if your parents were big fans of his. He is a great song writer. ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 3/31/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] onalighternote To: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov, Edwards, R.E. r...@leicester.ac.uk, histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 4:52 PM Yeah, hilarious.. I swear, I haven't heart THAT one before From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Edwards, R.E. r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:18:36 AM Subject: RE: [Histonet] onalighternote I wanted to ask how Elton was but thought that was a bit too easy... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AO Blade holder
Am I getting multiple mails or is it simply that David Waugh doesn't know how to use a listserv? From: David Waugh dwa...@kent.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 3:08:08 PM Subject: [Histonet] AO Blade holder I have a question regarding a AO disposable blade holder (Cat# 824). This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg The blade is tightened with a lever on the right that rotates a d-shaped cam. Two screws are used the set the tension that the cam places on the blade, once set, the cam will tighten and loosen the blade. The back of the holder is concave, and the front part convex, so that when tightened, the blade will bow. I have not been able to find any documentation on how tight the blade should be. It sounds like most holders have flat surfaces that clamp the blade, which I would think would have very different tightness requirements. Any ideas from someone who uses this type of holder on how the two screws should be set? Thanks, David Kent State University Department of Geology Kent, Ohio 44242 dwa...@kent.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] onalighternote
Then maybe we're really making fun of the other Bernie; we just didn't know it. :-) Perhaps you should get back to your histology and just plain stop making fun of anyone, for any reason. From: Pat Flannery pjfne...@duke.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:20:30 AM Subject: Re: [Histonet] onalighternote Then maybe we're really making fun of the other Bernie; we just didn't know it. :-) I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. From: Va Paula Sicurello vapat...@yahoo.com To: Jeanine (CDC/CCID/NCZVED)Bartlett j...@cdc.gov; R.E.Edwards r...@leicester.ac.uk;histonet@lists.utsouthwestern.edu; Bernie Taupin bernietau...@ymail.com Sent: Tuesday, March 31, 2009 7:01:14 PM Subject: Re: [Histonet] onalighternote Hey, we can't help it if your parents were big fans of his. He is a great song writer. ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 3/31/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] onalighternote To: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov, Edwards, R.E. r...@leicester.ac.uk,histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 4:52 PM Yeah, hilarious.. I swear, I haven't heart THAT one before From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Edwards, R.E. r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:18:36 AM Subject: RE: [Histonet] onalighternote I wanted to ask how Elton was but thought that was a bit too easy... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] onalighternote
I have just had s from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what s are. From: Edwards, R.E. r...@leicester.ac.uk To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] G 20 MEETING NEWS
Please refrain from discussing politics on this list. Edwards, R.E. wrote: Apparently Obama and Brown, with the reluctant compliance of Germany and China are aiming to rationalise the pay and conditions of laboratory workers worldwide, and hopefully if they succeed the histotechs in the U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] onalighternote
Please stop replying and use your own advice. Histology talk anyone? I'm curious- Why didn't you seem to feel this way about the first 3-5 replies that were sent out making fun of my name, completely devoid of histology talk? From: Robyn Vazquez vazqu...@ohsu.edu To: Bernie Taupin bernietau...@ymail.com; Edwards, R.E. r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:57:53 AM Subject: RE: [Histonet] onalighternote Please stop replying and use your own advice. Histology talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 6:55 AM To: Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what s are. From: Edwards, R.E. r...@leicester.ac.uk To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] G 20 MEETING NEWS
Sharon, You might like to know, then, that John McCain thinks histology matters http://lungcancer.einnews.com/article.php?nid=91 Beckham, Sharon wrote: I disagree. This is something I didn't know about and evidently it will effect our profession. No one enjoys getting political more than I do, but I don't consider this as being political talk. I do consider it being newsworthy and something I want to become informed about. Thanks for the heads up as I will want to follow up on this one. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Off topic posts
People get a life enough already Yes, thanks for your valuable, helpful insight, Florence. From: Florence Leomiti bat...@ohsu.edu To: Jennifer Anderson jander...@halozyme.com; Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 3:29:05 PM Subject: RE: [Histonet] Off topic posts People get a life enough already -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, April 01, 2009 12:23 PM To: Histonet Subject: RE: [Histonet] Off topic posts Oh my. If I had known my request for manuals would have sparked such diatribe, I would have withheld. Please stop at once - you're unnecessarily filling my Inbox. This is preposterous. The conversation should be over. I thought this list was monitored??? Jennifer Anderson The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 12:13 PM To: Baldridge, Lee Ann; Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Speaking of rude, here we have a perfect example: From: Baldridge, Lee Ann lhad...@iupui.edu Hey Bernie...Get over your self. Lee Ann Baldridge IUSM Indpls., IN. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Off topic posts
What were we talking about again? From: Joe Nocito jnoc...@satx.rr.com To: Bernice Frederick b-freder...@northwestern.edu; Bernie Taupin bernietau...@ymail.com; Bryan Llewellyn llewl...@shaw.ca; Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 7:58:10 PM Subject: Re: [Histonet] Off topic posts yeah, what she said - Original Message - From: Bernice Frederick b-freder...@northwestern.edu To: 'Bernie Taupin' bernietau...@ymail.com; 'Bryan Llewellyn' llewl...@shaw.ca; 'Histonet' histonet@lists.utsouthwestern.edu Sent: Wednesday, April 01, 2009 3:10 PM Subject: RE: [Histonet] Off topic posts How would we survive without those witticisms of JTT f we disallowed off topic posts? Joe? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 1:46 PM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? From: Bryan Llewellyn llewl...@shaw.ca To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn - Original Message - From: Bernie Taupin bernietau...@ymail.com To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce Nothing like yet another off-topic post to remind us not to post off-topic posts. Ahhh, sweet irony ;-) Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Off topic posts
You should consider going by Bruce. It suits you better, anyway, as far as I can tell.The last person I knew named Llewellyn was... that guy from The People's Court. Remember that show? http://en.wikipedia.org/wiki/The_People%27s_Court From: Bryan Llewellyn llewl...@shaw.ca To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 8:38:51 PM Subject: Re: [Histonet] Off topic posts My name is Bryan (rhymes with (Ian) not Bruce. I was just making a general comment. Bryan - Original Message - From: Bernie Taupin To: Bryan Llewellyn ; Histonet Sent: Wednesday, April 01, 2009 11:45 AM Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? -- From: Bryan Llewellyn llewl...@shaw.ca To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn - Original Message - From: Bernie Taupin bernietau...@ymail.com To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce Nothing like yet another off-topic post to remind us not to post off-topic posts. Ahhh, sweet irony ;-) Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Let's call a truce
At this point, I think about 20 or more different people have been involved in this thread! Would anyone else like to chime in with their two-cents about being on-topic or offer their inane peanut-gallery observations and silly tit-for-tat bickering like Paula here? From: Va Paula Sicurello vapat...@yahoo.com To: HistoNet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 1:10:30 PM Subject: [Histonet] Let's call a truce Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ford Royer
I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] onalighternote
Yeah, hilarious. I swear, I haven't heart THAT one before From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Edwards, R.E. r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:18:36 AM Subject: RE: [Histonet] onalighternote I wanted to ask how Elton was but thought that was a bit too easy... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet