RE: [Histonet] Jores, Klotz and that pesky chloral hydrate

[Betty Pollock]

We are a veterinary pathology teaching lab and we used Klotz with chloral 
hydrate to preserve our gross teaching specimens.  We switched to a Klotz 
recipe that does not use chloral hydrate because the chloral hydrate is hard to 
source and very expensive.  It is maybe not quite as good as the Klotz with 
chloral hydrate but seems to work OK.

Here is the recipe.
Sodium chloride 208 g
Sodium bicarbonate  375 g
Sodium sulfate  458 g
Formaldehyde, 37%   667 ml
Water   To make up to 50 L 

Regards,

Betty Pollock
Manager, Operations DSU
 
Faculty of Veterinary Medicine
University of Calgary 
Calgary, AB, Canada
Tel: 403-220-2806
FAX: 403-239-6984


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy
Sent: Thursday, November 20, 2014 2:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate

Hi All,
I am looking for a tissue fixative to preserve gross specimens for a veterinary 
pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's 
in the past, and Klotz has been recommended, but the chloral hydrate is a 
problem child due to its status as a controlled substance (eg disposal is a 
hassle).

Does anyone have any thoughts about just leaving the chloral hydrate out?
Or, an alternative fixative that doesn't have the chloral hydrate?  I have 
access to a refrigerator for samples, and would *hopefully* keep the specimens 
for a year or two.

Thanks a bunch,
M

--
Molly Murphy DVM, Ph.D
Assistant Professor of Veterinary Pathology College of Natural Sciences  
Mathematics University of Alaska Fairbanks
Office: (907) 474-1990
Fax: (907) 474-1932
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RE: [Histonet] SOP numbering for whole lab

[Betty Pollock]

Our histology SOPs start with H- (to distinguish them from other area SOPs) 
then each is assigned a unique chronological 4 digit ID number followed by the 
version number.

For example:  H-0001.00 where 0001 is the number for a specific SOP and .00 
refers to the original version.  The number for the first revision of this SOP 
would be H-0001.01.

It's simple and every SOP and revision has a unique number.  We keep master 
lists of the SOPs and the SOPs are under document control as well so each has 
its own history file.

Betty Pollock
Diagnostic Services Unit Manager
Faculty of Veterinary Medicine
University of Calgary 
Calgary, AB, Canada T3R 1J3
Tel: 403-220-2806



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: March 4, 2013 3:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] SOP numbering for whole lab

Help?  What kind of formula do you use when numbering and coding your SOPs for 
the lab?  Is there an easy way to keep them sorted and in line with all the 
regulatory bodies to help on inspection days?
 
Any suggestions welcome--we're starting from scratch.
 
Cheryl


 
 
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