[Histonet] RE: Frozen Section Help
Did you cryoprotect the tissue before you cut it? JB Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173 Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, November 08, 2010 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 84, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. cytotechnology (Mimmo Pozzuoli) 2. RE: SPAM-LOW: [Histonet] shrinkage during IHC (Patsy Ruegg) 3. RE: SPAM-LOW: [Histonet] cytotechnology (Patsy Ruegg) 4. Frozen section ...HELP (louise renton) 5. Re: Frozen section ...HELP (Lee Peggy Wenk) 6. RE: shrinkage during IHC (Kuhnla, Melissa) 7. RE: Frozen section ...HELP (Monfils, Paul) 8. Distance Learning (Phyllis Thaxton) 9. Microwave processing effect on DNA/RNA (Matt Brooks) -- Message: 1 Date: Sun, 7 Nov 2010 13:36:09 -0500 From: Mimmo Pozzuoli mimmopozzu...@gmail.com Subject: [Histonet] cytotechnology To: histonet@lists.utsouthwestern.edu Message-ID: aanlktim4e0d0+lofa=wtkcprr+thep7h3ew7-b=tk...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Are any histotechs out there performing FISH or automated cyto procedures in their labs? If so, are your pathologists having to screen and act as the cyto supervisors? What are the guidelines on this? Can a histotech perform cytoprep functions, or is the slide screening integral to the position? -- Message: 2 Date: Sun, 7 Nov 2010 13:20:58 -0700 From: Patsy Ruegg pru...@ihctech.net Subject: RE: SPAM-LOW: [Histonet] shrinkage during IHC To: zodia...@comcast.net, histonet@lists.utsouthwestern.edu Message-ID: 9109304a049d40f889c150360425e...@prueggihctechlt Content-Type: text/plain; charset=us-ascii You should have the same morphology from the IHC slides done outside that you get from your HE, do you provide them with slides or the Block? The only thing I can think of is if they airdry after IHC instead of going thru alcohols and xylene to coverslip (if they use AEC or ap/red they may airdry), I have seen cell shrinkage from airdrying after IHC occasionally. Something else just occurred to me, if they over heat during HIER I suppose this could happen, but I have not seen it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Saturday, November 06, 2010 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (HE, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Sun, 7 Nov 2010 13:55:42 -0700 From: Patsy Ruegg pru...@ihctech.net Subject: RE: SPAM-LOW: [Histonet] cytotechnology To: 'Mimmo Pozzuoli' mimmopozzu...@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: fcfb1eba62d341d29251230913eb0...@prueggihctechlt Content-Type:
[Histonet] RE: Autofluorescence
Richard, we had trouble with autofluorescence in brain tissue of the elderly. We have found that 1% Sudan Black B in 70% EtOH for 2 minutes before the secondary antibody works very well. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, October 18, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Re: Histonet Digest, Vol 83, Issue 20 (Weems, Joyce) 2. RE: AFB (Weems, Joyce) 3. RE: Re: Histonet Digest, Vol 83, Issue 20 (histot...@imagesbyhopper.com) 4. Re: AFB (Lee Peggy Wenk) 5. autofluorescence (Edwards, Richard E.) 6. RE: IHC FILD (Stephanie Rivera) 7. (no subject) (Debora Probst) 8. Eosin (sgoe...@xbiotech.com) 9. Block release (Bernice Frederick) 10. RE: Block release (Helen Fedor) 11. RE: Block release (wanda.sm...@hcahealthcare.com) 12. Re: Eosin (Rene J Buesa) 13. clinical trial blocks (Tench, Bill) -- Message: 1 Date: Sun, 17 Oct 2010 18:46:54 -0400 From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 To: histot...@imagesbyhopper.com histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e16403a6810...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -Original Message- From: histot...@imagesbyhopper.com [mailto:histot...@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the widget count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -Original Message- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, Weems, Joyce jwe...@sjha.org wrote: CMS/NCCI Update Dated October 1, 2009 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Thursday, October 14,
[Histonet] Histokinette 2000
I have a Leica Histokinette 2000 that needs one beaker too be usable. Does anyone know where I can buy one of these? IMEB, inc does not have them. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edumailto:jabiederm...@uams.edu Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
