Re: [Histonet] [External] Expiration date after opeing a reagent

[Bitting, Angela K. via Histonet]

This sounds absurd to me.

-Original Message-
From: Paula via Histonet 
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent

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Hello,



We just got CLIA inspected and had no deficiencies. The inspector did say he 
wants to see us write down on any reagent containers when it was opened, and 
write down the new expiration date.



The containers have the expiration date on them from the manufacturer, but the 
inspector said when the container is opened, the expiration will change and we 
need to write the date on the container under the opened date.



I've never heard of this requirement and I would like to ask if you have.
He said if the vendor does not give us any written documentation of when a 
reagent would expire after it's opened, then we should seek an alternate vendor 
who can.



Any comments, etc. are greatly appreciated.



Paula Lucas

Bio-Path Medical Group



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Re: [Histonet] [External] Re: Coffee at the desk

[Bitting, Angela K. via Histonet]

Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the 
VIP without fear of admonishment.

-Original Message-
From: Jay Lundgren via Histonet 
Sent: Friday, June 2, 2023 3:34 PM
To: Piche, Jessica 
Cc: Histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] Coffee at the desk

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Next thing you're going to be telling me it's not OK to embed with a 1 foot 
tall bunsen burner open flame and a Coke on the cold tray!

On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> It also doesn't set a good impression for everyone else in the lab
> since they can't have a coffee at their stations. If it could be done,
> I wouldn't want everyone else's coffees on my desk either,  but I'd
> have to because I'd want to make it fair to everyone. If only it was safe for 
> all of us
>
> Have a great weekend everyone!
>
> Jessica
>
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory Histology Team Leader
> 203-573-7167
> 
> From: McLaughlin, Stacy L. via Histonet
>  >
> Sent: Friday, June 2, 2023 6:09 AM
> To: Hannen, Valerie ;
> Histonet@lists.utsouthwestern.edu 
> Subject: Re: [Histonet] Coffee at the desk
>
> [EXTERNAL MSG]
>
> It is not allowed.  The area you want food/drink has to be completely
> separate- floor to ceiling walls and a door.
>
> Stacy McLaughlin, HT(ASCP) QLScm
> Histology Supervisor
> Cooley Dickinson Hospital
> 30 Locust Street
> Northampton, MA 01060
> Office:  (413)582-2019
> Lab:  (413)582-2179
> smclaughl...@cooleydickinson.org
>
>
>
>
> -Original Message-
> From: Hannen, Valerie via Histonet 
> Sent: Thursday, June 1, 2023 3:40 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Coffee at the desk
>
> External Email - Use Caution
>
> Is there any regulation/ reason why a Histology supervisor whose desk
> is in the department and the desk area is taped off and designated as
> a "clean area (  meaning no chemicals or specimens cross the taped off
> area) can't have a closed coffee cup at their desk??  We are having a
> debate in our Lab and I wanted to get a consensus.
>
> Thanks in Advance,
>
> Valerie
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish
> Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333  Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
>
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Re: [Histonet] [External] Clarification about Immunohistochemistry

[Bitting, Angela K. via Histonet]

We use an Azure B counterstain. Melanin has a greenish tinge after 
counterstaining.

-Original Message-
From: jayalakshmy p.s via Histonet  
Sent: Monday, April 11, 2022 11:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Clarification about Immunohistochemistry

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hai all Histonetters
 Please anybody clarify my this doubt if possible.
When doing Immunohistochemistry for confirmation of Melanoma with DAB 
chromogen(brown color)the interpretation is not possible because of obscuring 
by the dense melanin pigment. We dont have any other color chromogen. I tried 
ihc after bleaching but the section gets detached even from charged slides. Is 
there any other effective way to do this?
Thanks in advance
Regards
Dr. P S Jayalakshmy
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Re: [Histonet] [External] Re: Another Dispenser Failure

[Bitting, Angela K. via Histonet]

Is there any other way! :-O

Sent from my iPhone

> On Feb 28, 2019, at 3:30 PM, Mark Tarango via Histonet 
>  wrote:
> 
> I hope everyone is using on-slide controls :-)
> 
> On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Another one!!!
>> Our Her2 antibody dispenser failed, LOT #E22628
>> This one "supposedly" FDA approved.
>> Roche, why do you continue to lie to consumers of your product?  You claim
>> you've "fixed" the problem but your Ventana dispensers DON'T WORK!
>> This is patient care!  Why don't you care about the customers and patients
>> you are supposed to be serving
>> Shame on you.
>> 
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> 
>> -Original Message-
>> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
>> histonet-requ...@lists.utsouthwestern.edu]
>> Sent: Thursday, February 28, 2019 1:00 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: Histonet Digest, Vol 183, Issue 23
>> 
>> 
>> Today's Topics:
>>   1. H Staining question (Charles Riley)
>>   2. Re: H Staining question (Jay Lundgren)
>>   3. FYI- Roche Ventana users (Cassie P. Davis)
>> Message: 3
>> Date: Thu, 28 Feb 2019 15:23:16 +
>> From: "Cassie P. Davis" 
>> To: histonet 
>> Subject: [Histonet] FYI- Roche Ventana users
>> Message-ID: 
>> Content-Type: text/plain; charset="iso-8859-1"
>> 
>> Hi Histoland,
>>I am biting my tongue HARD and just letting you know so it doesn't
>> happend to you. I just got off the phone with Roche here is the heads-up.
>> If one of their anitbody dispensers fails DO NOT put the antibody in one
>> of their prep kits, as soon as you do they consider it off label use.
>> Call customer service immediately and have them overnight a replacement!
>> Cassandra Davis
>> Histology Technician
>> AP Laboratory
>> 302-575-8095
>> Email:  cda...@che-east.org
>> 
>> 
>> 
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>> 
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[Histonet] NOTCH2 help

[Bitting, Angela K. via Histonet]

Is anyone running NOTCH2 on Bond III OR Benchmark Ultra? Can I take a peek at 
your protocol and antibody selection?


Sent from my iPhone

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Re: [Histonet] [External] Re: Automated Slide Stainers

[Bitting, Angela K. via Histonet]

There are slide basket inserts to accommodate that sized slide for Sakura 
stainers. The DRS2000 for example. Check out this web page.

http://www.tedpella.com/histo_html/slides-large.htm


-Original Message-
From: Atoska Gentry via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, August 05, 2016 11:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] Automated Slide Stainers

Hello, one of my department's researchers is in need of an automated slide 
stainer which accommodates 3"x2" microscope slides. If you have experience with 
such will you please contact me at your earliest convenience? Regards, Atoska 
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Re: [Histonet] [External] Crystals in PAS from Ventana

[Bitting, Angela K. via Histonet]

Hi Mark,
 We still have this issue after two years of complaining to Roche.  We've had 
multiple TAS's come in and we've sent slides to Tucson. They've gone over the 
instruments, etc. Long story short, their fix was adding something to their 
package insert that says "Schiff's Reagent is known to contain some needle-like 
precipitate. At expected levels, this precipitate should not affect assay 
performance". Good luck.

-Original Message-
From: Mark, Danielle via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, July 20, 2016 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Crystals in PAS from Ventana

I am wondering if anyone else has been having issues with the PAS stain.  The 
crystals are not rinsing off of the slide using the benchmark special stainer.  
We are on our 3rd lot number, no change.  We have had our tubing changed and 
our instrument checked to make sure the rinse is appropriate.  This problem is 
causing our lab great frustration.  Any feedback would be appreciated.


Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories
8901 W Lincoln Ave. | West Allis, WI 53227
O: 414.328.6235 | F: 414.328.6237 |
danielle.m...@aurora.org

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[Histonet] curiosity may kill me

[Bitting, Angela K. via Histonet]

I'm curious as to why we heat the Auramine Rhodamine when we are staining 
tissue sections but our Micro lab stains at room temp.  It is because "it's 
always been done that way"?
Thanks group,
Angie






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Re: [Histonet] Histonet Digest, Vol 150, Issue 2

[Bitting, Angela K. via Histonet]

This seems to be the trend of society in general these days.
No manners.

-Original Message-
From: Hudson, Pamela R ASHVAMC via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, May 04, 2016 7:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2

OMG  This is terrible.  I can't believe I belong to a group of such 
unprofessional people.

Pamela R. Hudson, HT (ASCP)
Charles George VA Medical Center
Pathology Department
1100 Tunnel Road
Asheville, NC   28805
828-298-7911   x5747
pamela.hud...@va.gov

-Original Message-
From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 03, 2016 4:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2

Good to know that I am not the only on that think Rene is negative to others.  
Hey Rene it's not just one person that thinks your a dirt bag.


From: histonet-requ...@lists.utsouthwestern.edu

Sent: Tuesday, May 3, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2

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Today's Topics:

   1. No More Blog Posts --  Over and Out! (Lester Raff MD)
   2. IHC positive controls (Cindy Bulmer)
   3. Re: No More Blog Posts --  Over and Out! (Rene J Buesa)
   4. Fwd: (Linda Margraf)
   5. Re: No More Blog Posts --  Over and Out! (Manfre, Philip)
   6. Re: IHC positive controls (Morken, Timothy)
   7. Re: No More Blog Posts --  Over and Out! (Boyd, Debbie M)
   8. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
   9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
  10. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
  11. Re: Fwd: (Rene J Buesa)
  12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
  13. PAS/Decal Question (Marcum, Pamela A)


--

Message: 1
Date: Mon, 2 May 2016 17:39:28 +
From: Lester Raff MD 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
 
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local>

Content-Type: text/plain; charset="us-ascii"

To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a
line at les.r...@post.com   The mailing list
is never used for any purpose other than announcing a new blog post. Be sure to 
let me know you are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203



--

Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8


Hello Histoland,

I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?

1)  Cut (serial sections, stain the last slide for bugs) and oven time
(60) for 1 hr.
 then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time and put 
slides
 directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.

Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX






--

Message: 3
Date: Mon, 2 May 2016 18:22:59 + (UTC)
From: Rene J Buesa 
To: Lester Raff MD ,
"'histonet@lists.utsouthwestern.edu'"

[Histonet] Auramine Rhodamine staining

[Bitting, Angela K. via Histonet]

Hi Histonet Colleagues,

I'm surveying Histology labs about Auramine-Rhodamine staining for acid fast 
organisms.
If you'd like to help me by participating in my survey please follow the link 
below.
Thank you in advance for your responses.

https://www.surveymonkey.com/r/7JPL5K2


Angie

Angela K. Bitting, HT(ASCP),QIHC
Analytical Specialist, Histology
Geisinger Health System
100 N. Academy Avenue
Danville, PA 17822-1920
t. 570-214-9634
f. 570-271-5916



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[Histonet] BCL-10 for IHC-P

[Bitting, Angela K. via Histonet]

Has anyone been able to optimize an antibody to BCL-10 using Ventana's 
Ultraview detection?


Angie



IMPORTANT WARNING: The information in this message (and the documents attached 
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prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.

Geisinger Health System utilizes an encryption process to safeguard Protected 
Health Information and other confidential data contained in external e-mail 
messages. If email is encrypted, the recipient will receive an e-mail 
instructing them to sign on to the Geisinger Health System Secure E-mail 
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[Histonet] NHPD

[Bitting, Angela K. via Histonet]

Is it me or does anyone else think $20 is a lot of money for a T-shirt?  The 
Histotechnology Professionals Day T-shirts on NSH'S website seem over-priced.

Angie




IMPORTANT WARNING: The information in this message (and the documents attached 
to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
distribution or any action taken, or omitted to be taken, in reliance on it is 
prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.

Geisinger Health System utilizes an encryption process to safeguard Protected 
Health Information and other confidential data contained in external e-mail 
messages. If email is encrypted, the recipient will receive an e-mail 
instructing them to sign on to the Geisinger Health System Secure E-mail 
Message Center to retrieve the encrypted e-mail.
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[Histonet] Phosphorylase stain for muscle

[Bitting, Angela K. via Histonet]

Can anyone tell me what kind of glycogen to use for this? Sigma's website has 
multiple choices. Thanks.

Angie



IMPORTANT WARNING: The information in this message (and the documents attached 
to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
distribution or any action taken, or omitted to be taken, in reliance on it is 
prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.

Geisinger Health System utilizes an encryption process to safeguard Protected 
Health Information and other confidential data contained in external e-mail 
messages. If email is encrypted, the recipient will receive an e-mail 
instructing them to sign on to the Geisinger Health System Secure E-mail 
Message Center to retrieve the encrypted e-mail.
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