[Histonet] GI samples - Grossing Checklist
We are in the process of training a technician for GI grossing . If you have a checklist for this training and willing to share, I will appreciate very much your help. Thank you. Lin. Lin S. Bustamante, BSc, HT(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A University College Station, Texas 77843-4458 Phone (979)845-3177 Fax (979)458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Microtome RM2255
We are looking for a Leica RM2255 automated microtome gently used or a demo. Thank you. Lin. Lin S. Bustamante, BSc, HT(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A University College Station, Texas 77843-4458 Phone (979)845-3177 Fax (979)458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MEDITE Microtome
We are looking into purchasing new microtome for our laboratory. At NSH I saw this company and we are interesting to know the opinion of current users. We are looking into the fully automated one. Thank you very much. Lin. Lin S. Bustamante, BSc, HT(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A University College Station, Texas 77843-4458 Phone (979)845-3177 Fax (979)458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Advice regards NaDH and SDH stains?
Is anyone out there able to help this student? We don't perform, less analyze these stains. Thank you very much. Lin. +++ NaDH and SDH stains We are planning to perform magnetic resonance spectroscopy on the cranial sartorius and vastus lateralis muscles of control dogs and dogs with Golden Retriever Muscular Dystrophy to evaluate for differences in the concentration of various metabolites and then compare this with NaDH and SDH stains of biopsy samples from the same sites. However, I am having some difficulty determining how to compare them because I do not know how these stains are normally analyzed; do you employ a grading scale based on the severity of change? I am trying to confirm which statistical analyses to apply to compare the spectroscopic findings with the histological staining characteristics and thus really need to figure out exactly how the stains will be interpreted! I have been reading the literature to see how NaDH / SDH staining characteristics correlate with muscular dystrophy but it is proving a struggle. I was hoping you might be able to recommend a reference text? Thank you so much in advance. If there is someone else you would prefer I contact about this then please do let me know. Thanks again, Sarah ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glyco Kit
If you do Plastic embedding from a Kit, could you please share your experience? Please mention Kit name in your response. Thank you very much. Lin. Lin S. Bustamante B.Sc. H.T.(ASCP) Research Associate Texas A University College of Veterinay Medicine VIBS Histology Laboratory Supervisor Room 107 VMA College Station, Texas 77843-4458 (979)845-3177 (979)458-3499 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Hyde-Away
Can someone help her please? Thank you very much. Lin Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From: Coral Gonzalez [mailto:rayengonza...@yahoo.com] Sent: Friday, August 07, 2015 9:28 AM To: Bustamante, Lin Subject: Hyde-Away Hello, Please when you can, ask on Histonet about the ppm legal limit for draining formalin neutralized with Hyde Away. Thank you, Rayen Gonzalez B.S., HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Distain Masson Trichrome
Could you please let me know what % of Ammonia water is used for distain Masson Trichrome? Or what % is most common used? Thank you. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pH measurements for tissue
Does anyone knows how to measure pH in tissue? Any help will be greatly appreciated. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Colloidal Iron vs Alcian Blue
Hello, I would like to know if it was colloidal iron was more intense and also more specific than alcian blue or more intense but less specific than alcian blue? I have conflicting information regarding this stain. Thank you for your help! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hyde Away products
Does anyone uses Formalin Neutralizer Hyde Away from Fisher?. Do you do any test to figure out if is ready?, all neutralize? Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Use of Polar Heat Pad inside Microwave Processor
I need a good, reliable company to buy from. This pad is not cheap. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TSH new worshop submission
Could you please let us know WHEN and HOW to submit a workshop for the next TSH? Thank you very much for the information. Lin Bustamante and Rayen Gonzalez Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave Processor
I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big issue, if anyone knows about a cheap but very reliable I would like to hear about it PLEASE! Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) CTDDA Histology Lab Supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Texas Society for Histotechnology
Is there going to be a TSH meeting this year? PLEASE !!! I need to find out. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Green stain for Fat
Does anyone knows any fat stain were the fat stains green? Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pituitary gland
Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin Neutralizer
We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing. ..... Isopropanol
If your lab does Microwave processing using Isopropanol. How do you disposed this reagent? Thank you. Lin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gastro prices
For Gastric Biopsy, I need to know how much other labs are charging for: - HE slide - Unstained slide - Special stain Giemsa and Ab/PAS Thank you very much. Lin Bustamante. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about waste storage temperature
Can I store (Xylene substitute, Isopropanol and Formalin) each, separated in 5 gallons (closed with a tight lid) plastic containers placed inside spill tray in a room that the temperature can rise up to 40°C in the summer? One container at the time, before it gets pick up by waste company. Thank you. Lin. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fo a small GI lab.... Question
How do you store your waste before been picked up by any company. I need to know what is acceptable by CAP Inspectors; temperature for the room. Our containers are outside lab, etc. Any information will be very much appreciated. Thank you. Lin Bustamante ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Short time Formaldehyde exposure
Does anyone knows a Co. in Texas that does on site testing for short time formalin exposure? Short time of 15 min or less. Thank you. Lin Bustamante ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave verification
Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microwave verification
Thank you so much. You are all great. Lin :-) From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Wednesday, May 23, 2012 3:33 PM To: Weems, Joyce K.; Bustamante, Lin; histonet@lists.utsouthwestern.edu Subject: RE: Microwave verification Ok - attachment didn't work. Whoever wants the procedure, just let me know it is ok to send separately! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 23, 2012 4:27 PM To: 'Bustamante, Lin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microwave verification Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ✓ Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: “Reproducibility” is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ✓ Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ✓ Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ✓ Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you
[Histonet] Microtome Reicher-jung 2030
I am looking to buy 2 of this microtome in very good condition please. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Lab Supervisor College Of Veterinary Medicine Texas AM University Phone (979) 845-3177 Fax (979) 458-3499 lbustama...@cvm.tamu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
