[Histonet] Eosin
Hi, I have been staining fish tissues fixed in Davidsons with H, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. Thank you. Elizabeth M. Cameron, HT(ASCP), QIHCCM Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Film Coverslipper
Just wondering if anyone using Sakura film coverslippers has noticed streaks along the edges of the slides. I am sure they are caused by the adhesive leaking out and being wiped down the slide when we wipe the xylene off, but I am wondering if anyone has any suggestions for eliminating the streaks. We have tried letting the slides air dry and still get waterspots where the xylene was. Thanks. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding Center Temperature
Hi, I am curious about which temperatures people are tracking on their embedding centers for CAP, and how they are tracking them. If you are tracking the temps for the forceps and hot and cold plates, are you using the internal thermometer or measuring the temperature another way? Thanks. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] North East Laboratory Conference
There is a North East Laboratory Conference that is held in Portland, Maine every year, and each year I notice a lack of speakers pertaining to the fascinating subject of histology. I have taken it upon myself to recruit a few histology speakers, and I am hoping to get some talks set up for October 20, 2015. If you are interested or if you would like to recommend a speaker, please send me a message directly, and I will pass along the details of the conference. Thank you! Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Primera Signature and Microbiopsy Cassettes
I am looking for recommendations for microbiopsy cassettes to use on the Primera Signature printer. I have two different types (Leica LP C and Simport Micromesh), and neither of them print as clearly as the standard Tissue Tek cassettes. Thanks in advance. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Primera Signature and Microbiopsy Cassettes - addendum
Just wanted to add that I have used Tissue Tek Mesh biopsy cassettes in the past and experienced issues with processing. I would also be interested in hearing from people who use the Mesh either successfully or unsuccessfully to determine whether that is an option. Thanks again. From: Cameron, Elizabeth Sent: Thursday, February 19, 2015 1:32 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Primera Signature and Microbiopsy Cassettes I am looking for recommendations for microbiopsy cassettes to use on the Primera Signature printer. I have two different types (Leica LP C and Simport Micromesh), and neither of them print as clearly as the standard Tissue Tek cassettes. Thanks in advance. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Giemsa - smears vs. paraffin sections
Happy New Year, Histonetters! I have always been taught that a Wright-Giemsa stain was strictly for smears, and a different modification (i.e. May-Grunwald) should be used for tissues. I was recently asked what the reasoning was, and to be honest I am not sure. I understand that tissue stains differently from smears, and I would imagine timing would be different, but is there a reason the solutions are different? Very curious. Thanks! -Liz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Paraffin
I am still trying to figure out what I need to do for validation for CAP. Do I need to revalidate IHC and/or special stains if I change the paraffin on the processor? The paraffin I have in mind is supposed to work faster than the one we currently use for infiltration, so we may need to adjust times. Thanks. From: Boyd, Debbie M [dkb...@chs.net] Sent: Wednesday, April 16, 2014 10:17 AM To: Cameron, Elizabeth; histonet@lists.utsouthwestern.edu Subject: RE: Paraffin We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, April 16, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin
Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
