[Histonet] Myophosphorylase
Hello Histoland, This is a two part question for all those who do muscles. 1.Is anyone having problems ordering glucose-1-phosphate? 2.Does anyone have a method for myophosphorylase that doesn't require glucose-1-phosphate? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic Hospitals Hamilton, ON, Canada 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PFK's on muscle biopsies
Happy Friday !! For years now our PFK staining has been inconsistent and I'm hoping someone wouldn't mind sharing their no fail PFK method so I can make my neuropath's happy again.?? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic Hospitals 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Isopentane storage-the polling is open
We store our open bottle in an explosion proof fridge and unopened at room temp in a flammable storage cabinet Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SDH methods for muscles
I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ATP 10's for muscle biopsies
I'm having issues with the ATP 10's we run on our muscle biopsies and am hoping those who are familiar with this stain are willing to share their methods?! Any help or advise is greatly appreciated. Thanks Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Training first time cutters
It's been awhile since we've had to train someone to cut from scratch and I need your help to figure out two things: 1. how long should it take a new cutter to cut 20 blocks, no levels, nothing fancy, just one HE per block. 2. what's the average length of training for the above to be accomplished? how long is long enough? Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celeb...@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flame substitute for embedding
Hello Histoland!! We have just been told we are no longer allowed to use alcohol burners while embedding and we are to find a safer substitute, something to do with no liking an open flame in the lab. Other than incinerators or 6 pairs of forceps at each centre, we've run out of ideas so we are asking all you experts out there what your current practice is.. oh.. this change needs to be done by this Friday!! Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: [EMAIL PROTECTED] This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
