[Histonet] IHC

[Chakib Boussahmain via Histonet]

Greetings Histonet community,

I'm interested in learning if anyone has experience withperforming Von 
Willebrand factor and alpha smooth muscle actin IHC staining onpig tissues 
(FFPE). If you do, could you please share the protocol along withdetails about 
the antibodies used, including ordering cat# and clones?

Any assistance you could provide would be immensely appreciated.

Best regards, 
Chakib
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[Histonet] Bone samples

[Chakib Boussahmain via Histonet]

Hi guys,

 I hope this messagefinds you well. I am currently working on a study involving 
bone samples thathave been treated with slow decal and embedded in paraffin. I 
am facingchallenges in obtaining nice sections, and I was wondering if you 
could providesome guidance or recommendations.

Thank you in advance for your help!
Chakib
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[Histonet] Tissue Quality

[Chakib Boussahmain via Histonet]

Hi Guys,

We normally buy human tissue blocks to use them as controlsor to develop IF 
assays in our laboratory. However, most of these blocksappeared to be fixed or 
processed adequately. I am wondering if anybody has amethod to assess the 
quality of these tissue blocks besides H stain. Forinstance, is there an IHC 
marker or a panel of the markers that I can use as agood indicator to qualify 
these tissue blocks?

Thank you so much in advance for any suggestions!

Best

Chakib

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[Histonet] Harmony image analysis software

[Chakib Boussahmain via Histonet]

Hi Guys,I am just wondering if anyone is using Harmony image analysis software? 
or Opera phenix machine?I will appreciate any inputThank you in 
advanceChakibHistotechnologist
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[Histonet] Protocol or SOP of how to make Tissue microarray

[Chakib Boussahmain via Histonet]

Hi Histonetters,Does anyone have a written SOP or a protocol of how to make 
Tissue microarray that can share with me. Any input will be greatly 
appreciated.Thank you in advanceChakib BoussahmainHistotechnologist
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[Histonet] Human Tissue Blocks

[Chakib Boussahmain via Histonet]

Dear histoneters,I am working on some immunofluorescence multiplex staining/ 
optimization of the different kind of antibodies and I am wondering if someone 
can help me find human tissue block suppliers. I will need different type of 
tissue normal/disease and different organs.Any advice/ help you guys would give 
me would be much appreciated.Thank youChakib 
BoussahmainHistotechnologistCambridge MA
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