[Histonet] Deyemond-Diamont Knife
Hello everybody, I was curious, whether anybody of you has any experiences with Deyemond-Diamont Knifes, regarding durability etc. Apparently the company doesn't exist anymore, but we still have some knifes and I wanted to know what your opinions would be compared to other companies. Regards, Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Whole rat head histology
I want to do the same, with adult rats. doing paraffin sections seems to be the best option I guess Am 17.09.2014 18:02 schrieb Marcum, Pamela A pamar...@uams.edu: How old are the rats? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William J. O'Connor III Sent: Wednesday, September 17, 2014 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole rat head histology I have a fun project - processing a whole rat head, skull with brain, for serial sections to see a brain/skull congenital deformity.Anyone have any experience with this? I'd love it if you could share your protocol. Paraffin or plastic? Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mounting media for immunofluorescence
Hello folks, I am wondering which mounting medium für IF you are using in your daily routine. We are using Prolong antifade gold, but it is really really really expensive and I was wondering if there is a good alternative. I just ordered a sample of the Dako Fluorescence Mounting Medium so we can try that , but we are a little bit concerned about how long lasting the fluorescence signal would be. Well, what are your experiences so far. All the best Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quartzy
Hello everybody, we are currently thinking about a system to organize our lab-inventory and orders. Somebody recommended Quartzy to us (https://www.google.at/#q=quartzy+). Now I was wondering if anybody of you is using this system already, and what experiences you were having with it so far. Please share your thaughts. All the best Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PET Membrane Slides
Hello histonetters, does anybody of you have experience with PET Membrane Slices (Leica) for laser capture-dissection and how to coat them so that your slices won't swim off the slide? There is a Master Student in my group who would highly appreciate any help! Greetings Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] open source NIS ELEMENTS
Hello, we have a Nikon Eclipse E600 with a DS 2Mv camera, it was a present from another lab and the dongle for the Software is missing. So before I am contacting Nikon if it is possible to get a free/cheap additional dongle, I wanted to ask you if somebody knows a capable open source equivalent to the NIS ELEMENTS softare. Regards Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PBS and PB
Hu histoneters, we are currently standardizing our buffer protocols/recipes and I am wondering what recipes you are using for PBS and salt free PB. Would appreciate any kind of help for optimizing my database. All the best Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Luxol Fast Blue
Thanks everybody for the fast help!! Christina 2014-05-15 18:56 GMT+02:00 Rene J Buesa rjbu...@yahoo.com: If you are going to use Luxol Fast Blue for brain sections (its most frequent use) Goole the stain Luxol Fast Blue-Cresyl Etch Violet. This combination is much better. René J. On Wednesday, May 14, 2014 3:24 PM, Grantham, Andrea L - (algranth) algra...@email.arizona.edu wrote: There are several procedures online. Just google Luxol fast blue staining procedures. It is an easy stain to do. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luxol Fast Blue
Hello everybody, can anybody send me a Luxol Fast Blue staining protocol for mouse/rat brain (40µm slices) and spinal cord (10µm)? Any tipps and tricks are higly appriciated!! Thank you Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryoprotection and anti.freeze
Dear Members, I was wondering what cryoprotection protocols you are using for perfusion fixed tissues (30% succrose, 1:1 Tissue Tek/PBS etc). Why are you using what you are using and did you ever have any problems. Second, I wanted your opinion concerning leaving tisse in cryoprotection solution for several months and antibody reactivity and/or if somebody ever had problems with antibody reactivity on CPS (Glycerin, Ethylene Glycol, PO4 buffer and water). Thanks in advance Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryosectioning - still bubbles between slide and section, spinal cord
Dear members, first I wanna thank all the people who responded to my first email (Andrea, Mesru and Emily).Your tips were very helpful. Unfortunately I am still fighting agains bubbles/ wrinkles/bubble-shaped wrinkles between my slices and the slide. During the last couple of days I tried: i) to adjust the temperature of the chamber and the sample, seems like -16°C brings the best slices ii) to cool the slide by putting it into the chamber and warming it with my thumb just the second I held it towards the slice to adher iii) to warm the slides by putting them on a 37°C warm heating-plate after attachement iv) to moisten the slides with a little bit of bidest before letting the slices attach and put these slides on a 37°C heating plate afterwards...that was kind of desperate try to straighten the slices slowely v) 3 different intermedia before embedding the sample in pure Tissue Tek, a) 30% Succrose, b) half 30% succrose and half Tissue Tek, c) half Tissue Tek half PBS but...nothing really helped. I still have wrinkles aka bubbles. Tomorrow I am going the cut perfusion fixed rat spinal cord, hoping that this makes a difference. Anyway, I think I have some options left 1) trying to cut on a different mikrotome and 2) Leica promised to send me some slide/embedding solution/blade samples that worked for other groups. Can anybody think of something I havent tried yet? Besides of raising slide thickness and cutting for freefloating procedure? I would be so thankful for any ideas/suggestions/tips! Regards Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryosectioning, bubbles between slide and section
Hello members, I would need some help regarding cryosectioning of spinal cord. I am currently establishing cryosectioning on the cryostat (Leica CM1950) for this tissue and am having some problems. I am cutting 4% PFA fixed and succrose infiltrated spinal cords, embedded in Tissue Tek at 10µm and a temperature of approximately -16°C . I get more or less smoth slices but once I let them adhere to the slide -directly from the knife, after straightening it carefully with a brush - I get massive air bubbles between the slide and the slice. I have experience cutting on cryostats and with different tissues and never have had this problem before. I tried to change the temperature of the chamber and/or chunk and tried to warm the slide before adhering the slice and I tried to cool the slide. But it didn't help. Do you think changing from 30% succrose to a mixture of Tissue Tek/Succrose or even Tissue Tek/PBS would help? Does anybody have an advice? Regards Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
