Posting for my colleague, Jeff Gordon, because his post did not go through successfully:
"Suzie, your histonet post was forwarded to me by a colleague, and I thought I could help with your mast cell staining dilemma with pan-keratin. University of Pennsylvania published an article a while back about "undesirable" reactivity of cytokeratins that focused primarily on sentinel lymph node testing. In their study they found that when cytokeratin markers OTHER than strictly AE1/AE3 (with no other components) were used, other non-epithelial elements were being labeled that they deemed "undesirable." This entire article can be found here for your's and your pathologists' reference: http://www.archivesofpathology.org/doi/pdf/10.1043/0003-9985(2000)124%3C1310%3AUCIONN%3E2.0.CO%3B2 Based on these findings, and because another person in the thread also indicated that they may be dealing with the same issue, cervical tissue that was rich in mast cells was stained on a Ventana Ultra with cytokeratin AE1/AE3 ONLY as well as with cytokeratin OSCAR (which is another pan-keratin but with a lesser sensitivity to squamous epithelium than AE1/AE3) with CC1 mild heat retrieval and 16 minute heated primary incubation and Ultraview detection. To confirm the presence of mast cells in the tissue, both CD117 and tryptase were also stained on the same tissue with the same protocols. The results showed that though there was an abundance of mast cells present labeled by tryptase and CD117, there was no detectable staining of mast cells with either cytokeratin AE1/AE3 or with cytokeratin OSCAR, while the epithelial portion of the tissue stained with both cytokeratins (though the OSCAR was noticeably weaker because of the lower sensitivity to squamous epithelium). The stains can be seen here: http://www.cellmarque.com/cmc/AO_mastcellreactivity.php Hopefully this helps. If you have any questions, please contact us at your convenience." Thank you -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Tuesday, May 06, 2014 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory mill...@emhs.org<mailto:mill...@emhs.org> ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
