[Histonet] special stainers

2013-03-19 Thread Debra.Ortiz
Our histology laboratory is currently having problems with their
specials stains; in particular the silver stains. Can you please tell me
what instrumentation others in the field may be using for special
stains?
 
Thank you
 

Debra A Ortiz, BS, MT, HT (ASCP)
Technical Director, Anatomic Pathology
The University of Chicago Medicine
5841 S. Maryland Ave. | Rm. S-631, MC6101 | Chicago, IL 60637
Office: 773-702-8492
Pager: 8633

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[Histonet] possible AFB contaminant

2010-05-20 Thread Debra.Ortiz
Please help, we have had an increase in demands for repeats on our AFB stain. 
Our pathologists have been noticing what they believe are false positives on 
our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. 
Because of this problem, we have been doing side by side comparisons using the 
Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our 
DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.
 
Thanks Debi


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Thank you
University of Chicago Medical Center 


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[Histonet] Coverglass

2010-03-29 Thread Debra.Ortiz
We are currently trying to find a manufacturer that may sell cover glass
slips measuring 45 x 60. We found a box by Clay Adams, but can not find
that size anymore in their catalog.

 

Debra Ann Ortiz

Chief Medical Technologist

The University of Chicago Medical Center

Room E-602-A

5841 S. Maryland Avenue

Chicago, Il 60637

phone: 773.702.5237

 


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Thank you
University of Chicago Medical Center 

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[Histonet] Bone marrow processing on Peloris

2010-01-26 Thread Debra.Ortiz
Good morning,

I am currently looking at the possibility of running bone marrow cores
and clots on our Peloris. Has anyone had success with this, and if so
would you be willing to share your protocol? We use xylene on our runs.

 

Debra Ann Ortiz

Chief Medical Technologist

The University of Chicago Medical Center

Room E-602-A

5841 S. Maryland Avenue

Chicago, Il 60637

phone: 773.702.5237

 


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University of Chicago Medical Center 

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[Histonet] CJD procedures

2009-05-27 Thread Debra.Ortiz
We are looking to revamp our procedure for CJD and I would like to see
if there are different protocols out there. At this point we are
handling the whole process by hand, including melting the paraffin in a
melting pot. Do we need a separate oven for melting the paraffin and
heating the slides?

Thank you

 

 

Debra Ann Ortiz

Chief Medical Technologist

The University of Chicago Medical Center

Room E-602-A

5841 S. Maryland Avenue

Chicago, Il 60637

phone: 773.702.5237

 


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University of Chicago Medical Center 

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RE: [Histonet] (no subject)

2009-03-31 Thread Debra.Ortiz
Thank you for your very prompt response. I do agree that we are here for
the patient. 

Unfotunately I am under the gun to bring down costs and I thought that
might be one way. Thanks again, and look forward to other responses.

 

Debi

 



From: Lorraine Cornett [mailto:corne...@hotmail.com] 
Sent: Tuesday, March 31, 2009 9:36 AM
To: Ortiz, Debra [UCH]
Subject: RE: [Histonet] (no subject)

 

Yes,
Unfortunately(financially) it is current practice at our facility as
well.  As a preemptive measure, I keep a record of the number of all
unstained slides(total number only) thrown out on a monthly basis.  The
only alternative is to not cut the extra's and take a chance on not
getting the proper area on the slide for diagnostic stains.  I think
that patient care is of the utmost importance, and this probably is a
small price to pay in the grand scheme of things.


Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division,
Kingsport, TN 423 224-5793 fax 423 224-5349


 
 Date: Tue, 31 Mar 2009 09:30:18 -0500
 From: debra.or...@uchospitals.edu
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)
 
 We are currently handling all prostate, lung, heart, and breast
biopsies
 as fine needle biopsies (FNB). We cut 10 slides 3 of which are used
for
 HE's and the rest as unstained plus slides. What has happened is that
 we throw thousands of unused expensive slides a year because they are
 not used. Is there a general standard in practice out there for FNBs?
 Unfortunately, with the economy the way it is; and trying always to
find
 ways to bring down expenses this seems counteractive.
 
 
 
 Thank you
 
 Debi
 
 
 
 Debra Ann Ortiz
 
 Chief Medical Technologist
 
 The University of Chicago Medical Center
 
 Room E-602-A
 
 5841 S. Maryland Avenue
 
 Chicago, Il 60637
 
 phone: 773.702.5237
 
 
 



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 Thank you
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