Re: [Histonet] Instrument to instrument correlation

[Echague, Caren Ann - MGH via Histonet]

What was the vendor for your TMAs? I have been shopping around and trying to 
find the best ones. =)
Cae 


-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Thursday, November 01, 2018 10:44 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: Re: [Histonet] Instrument to instrument correlation

Hi Paula - 
I use duplicate microarrays (TMAs), each with at least 10 positive cases and 10 
negative cases, one TMA slide on each instrument for each antibody.
Passed CAP with no problems. Hope this helps. Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
  

 3. Instrument Correlation (Paula)
Date: Thu, 1 Nov 2018 08:01:32 -0700
From: "Paula" 
Hello everyone,
Could you recommend/suggest how the correlation between 2 IHC Bond instruments 
is performed and written out for them?
This has never been brought up before and I'm just starting my research.
Thank you in advance,
Paula




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[Histonet] p16 dilution

[Echague, Caren Ann - MGH via Histonet]

Hi Histonetters,
Does any of you use p16 Cintec from Roche/Ventana?  We wanted to dilute this 
antibody but wasn't sure how much to dilute it to.  Figured we could save a lot 
by doing so.  We have been staining our p16 with no current dilution.  Any 
suggestions will help.  If there are any other vendors that offer p16 other 
than Roche, that would be useful to know.
Thanks!

Cae Aguilar
Histology Supervisor
California


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Re: [Histonet] IHC validation

[Echague, Caren Ann - MGH via Histonet]

Where do you purchase your Tisssue Micro arrays? I know they are quite 
expensive.
We are currently running validations and using only a handful of positive 
cases. Most of them don't have ten positive and negative cases but as long as 
you can get a statement from the medical director, this should be CAP compliant.
Cae Aguilar, HTL (ASCP)
Histology Supervisor
7079802801

-Original Message-
From: Allan Wang [mailto:all...@biomax.us] 
Sent: Thursday, March 22, 2018 11:02 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC validation

How are labs validating for rarer biomarkers like ALK, ROS1, or MMR loss?
Following the guideline of 10 positive cases may be difficult.
I've seen other companies with control slides just with a few engineered cell 
lines as positive and negative controls. Is that enough for validation alone?

We are creating TMAs for validation of ALK and MMR loss (MLH1, PMS2, MSH6,
MSH2) so I am interested in knowing how many cases are desired.

Allan

Allan Wang
Lab Manager
US Biomax

On Tue, Mar 20, 2018 at 1:54 PM, Terri Braud via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Just another note:  You can order unstained tissue microarrays with 
> the prerequisite number of cases, both positive and negative, and 
> stain your validation all on one slide.  I've done this for years and 
> for 3 different validations of entire IHC platform changes, ranging 
> from 40 to over 100 antibodies each time.  Saves time and money.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Message: 2
> Date: Fri, 16 Mar 2018 06:54:30 -0700
> From: "Paula" 
> Subject: [Histonet] Antibody Validation CLIA
>
> Hello,
> We've been discussing about the quantity of slides to run as a 
> validation for IHC antibodies. We are governed by CLIA, and we would 
> like to know if there is a set number of slides to run for a 
> particular antibody we would like to bring in-house for Validation.  I think 
> CAP requires 20 slides..?
> And so we are asking if there is  a requirement with CLIA to run a 
> certain number of slides, or is it up to us (the laboratory director) 
> to decide how many slides to run for Validation/Verification.
> Thank you in advance
> Paula
>
>
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