Re: [Histonet] Histopathology text recommendations
Hi Gordon, I think you meant a histopathology atlas for slide reading, right? I am not a human histopathologist, but there are many such texts available, for example, see these at Amazon: https://www.amazon.com/s/ref=nb_sb_noss_1?url=search-alias%3Dstripbooks&field-keywords=histopathology Amazon.com: histopathology: Books<https://www.amazon.com/s/ref=nb_sb_noss_1?url=search-alias%3Dstripbooks&field-keywords=histopathology> www.amazon.com Online shopping from a great selection at Books Store. Also, several websites from medical schools offer virtual microscopy histoslide collections of different pathologies, such as: https://med.virginia.edu/biomolecular-analysis-facility/services/shared-instrumentation/aperio-scanscope-slide-scanner/catalogs-of-histology-and-pathology-virtual-slides-on-the-web/ <https://med.virginia.edu/biomolecular-analysis-facility/services/shared-instrumentation/aperio-scanscope-slide-scanner/catalogs-of-histology-and-pathology-virtual-slides-on-the-web/>http://www.mbfbioscience.com/iowavirtualslidebox <https://med.virginia.edu/biomolecular-analysis-facility/services/shared-instrumentation/aperio-scanscope-slide-scanner/catalogs-of-histology-and-pathology-virtual-slides-on-the-web/>https://www.amboss.com/us/knowledge/Virtual_histopathology_slide_box And others, just search in Google for "histopathology virtual slide box." Perhaps one of the pathologists on this list-serv can guide you to the best one(s) for your needs! Esther Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu<mailto:epete...@gmu.edu> <https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu>http://esp.gmu.edu From: Frazier, John via Histonet Sent: Sunday, May 20, 2018 5:31:01 PM To: Saundra Ellis Cc: histonet@lists.utsouthwestern.edu; Gordon Brown Subject: Re: [Histonet] Histopathology text recommendations Histotechnology: A Self-Instructional Text Sent from my iPhone On May 19, 2018, at 5:17 PM, Saundra Ellis < saundra.el...@floridawomancare.com> wrote: Anything written by Freida Carson. Saundra Ellis Histology Supervisor Florida Woman Care Laboratory Cell (360) 513-9665 (best contact) FAX (813) 433-5542 saundra.el...@floridawomancare.com From: Gordon Brown via Histonet Sent: Saturday, May 19, 2018 2:49:45 PM To: histonet@lists.utsouthwestern.edu Cc: Gordon Brown Subject: [Histonet] Histopathology text recommendations Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1GfcR_zVGSNZFprqljlK8MjuhxAYpfP_QQTByrhqt0Kr-8g_1tLjUE7U975eLICUinF2QuqNDTYEwySGvoO3MckIR7aPYIJWxWkfFU6PgoXls4px4v4M9ieidWRc2UKuyT_gFjkyGmhTAblsJg3p32hE-rwF5K0PHxrDz5Cg4bSDcrSvJZ8rkhqIExA4chiqHX9idd4CO3tF8ntTCKBPyR-WFdT-cjTfdI6JQfhohSMGkRty97ApMwmkp0rzHciE8aTB1k5jF8zh5zPogwnhPms9QMzen_fTLkSlXDcgeHqU__Se8UMaq0j1d1y1R540__zWnWuRd7Wrd55uLx4PyD7cE-KUdjirYuAHP4ymzLKMmf3h04IqBiGjHSIx3aLZDE4hlSsjEgYb5pldCRRZb7ZLEf-DHCk7Oqke-a_gRT2uERiFGVBXnweu_o5XU96pd/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: The information contained in this transmiss
[Histonet] Weigert's hematoxylin for IHC
Could someone advise me on whether Weigert's iron hematoxylin can be used as the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin because that will stain mucocytes in the coral tissues we are working with. If it can be used, would that be after all antibody steps have been done? Thank you! Esther Peters Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu<mailto:epete...@gmu.edu> <https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu>http://esp.gmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] For sale: Lab storage - Tissue-Tek flat tray cases
Tissue-Tek slide storage tray case. Capacity is 800 slides (50 trays of 16 slides each). These are hard to find in the US - model #4023. Slides store flat. Have 12 brand new still in boxes. $180 each. (703)342-7804 or katca...@ymail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Handline paraffin
However, I would be concerned if using Paraplast Plus (+) because it contains dimethyl sulfoxide (DMSO) to infiltrate the tissue faster. It can be absorbed through bare skin and you get a garlic taste in your mouth, making you wonder what else was absorbed into your body (DMSO is used for transdermal drug administration). We wear gloves when working with molten Paraplast +, but not for microtomy of the solidified blocks. Esther Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu From: Rene J Buesa via Histonet Sent: Monday, August 3, 2015 12:52 PM To: Johnson, Carole; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Handline paraffin It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or "irritating" substance. It is just an oil of high molecular weight (mineral oil)René On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" wrote: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Cutting of Paraffin Blocks
I start my students with plain paraffin blocks (no tissue in them) that they make themselves, to start. After they have worked with those, looking at how adjustments, then icing, then moving sections to the waterbath work, then I give them old practice blocks. This seems to be less intimidating than starting with tissue (although I think that is how I learned!). Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cooper, Brian Sent: Wednesday, March 25, 2015 1:17 PM To: Laurie Colbert; Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cutting of Paraffin Blocks Completely agree. Give them practice tissue blocks because it's expected that they WILL destroy them during the early stages of their learning process. If I can go one further here--when these students have demonstrated acceptable microtomy technique (and when you feel comfortable), be sure to teach them how to realign their microtome's orientation head/specimen clamp to the surface of blocks that have been previously cut.I cannot tell you how many histotechs I've met in my career who didn't possess this skill and simply "refaced" these blocks. One even yelled at the Biomedical Maintenance tech when he serviced her microtome and readjusted the orientation! I have a student in our lab right now, and tasked her with providing me 5 H&E's from each of the practice blocks I gave her. Here's the catch. In between each section that she collects, she is supposed to completely misalign the specimen head, then realign to the surface of the block. I told her that all five H&E's from each block should look relatively similar by the time she's done, and that there should be no appreciable tissue loss. While this might seem like a torture test for beginners, it will only serve them (and ultimately the patients they serve) so well in the future. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, March 25, 2015 9:49 AM To: Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cutting of Paraffin Blocks Heck no, crack that whip! Give them practice tissue that isn't important so they won't be afraid of ruining something. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, March 25, 2015 9:26 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting of Paraffin Blocks Hi, How are you guys doing? I hope you are all doing well. Please I have two histology trainees and they have spent more than six months in the histology lab and they cannot cut blocks yet. They are comfortable with filing slides and blocks. But I have been telling them that they have to start cutting now. My question now is am I too hard on them? Best regards, Adesupo Adesuyi == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may cont
[Histonet] Re: Recycling alcohol
American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu From: histonet-boun...@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Conf
[Histonet] Anyone doing insect histology?
?Do any of you do insect histology/histopathology? We are looking for someone to process the insects (primarily bees) and then read the histoslides and provide a report on the findings. Appreciate any assistance you can provide, or potential contacts! Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University Fairfax, VA 22030- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: paraffin
The nice thing about those is that "sticky mats" will also collect the dust from shoes; I found that these stop a lot of dust coming in at the outside door entrances, as well as having them to collect paraffin under the embedding center, in front of the tissue processor and fume hoods, and under the microtomes. It is amazing how much dust and dirt can come in with people's shoes! Esther C. Peters, Ph.D. Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu From: histonet-boun...@lists.utsouthwestern.edu on behalf of Walter Benton Sent: Wednesday, August 13, 2014 9:05 PM To: Algeo, Lacie A; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: paraffin You may want to purchase the large tack mats for staff to walk over when going in and out of your embedding area. These come in blue, white, or gray: http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=5264212&distype=0&highlightProductsItemsFlag=Y&fromSearch=1&searchType=PROD&hasPromo=0 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A [lacie.al...@providence.org] Sent: Wednesday, August 13, 2014 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin Hi all, I am dealing with an issue with slippery floors outside of our Histology department. The concern is that it is from paraffin scraps tracked on the bottoms of peoples shoes. The floor is cleaned every night and an anti-slip agent applied. I have tested whether our paraffin makes the floors more slippery and it actually makes it harder to slipalmost tacky. I really do not think it is our paraffin. I think it is a thin layer of dust that settles on the floors after they are cleaned. I have seen this happen in other places. Is anyone dealing with this? Any insight? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.al...@providence.org<mailto:lacie.al...@providence.org> This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HTL certification
Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memorizing is not what you should be doing. You need to understand concepts, so that when you need to troubleshoot problems you will be able to think through things, rule some things out, and make sense of the situation. I see this all the time with my students, they forget things they memorize, but then they finally understand things and can figure things out. One of the new teaching tools is having students prepare "concept maps," to see the relationships of topics and terms, and these linkages will help you in the long run. For histology examples, see: http://www.biologycorner.com/anatomy/tissues/tissue_concept_map_samples.html I don't know of any concept maps for histotechnology on the web, but I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epete...@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cecy Stan Sent: Monday, May 19, 2014 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL certification Hello everyone, I'm starting to prepare for my HTL certification (I am very nervous and anxious but also very excited about this decision to go for it). I was just curious to know how you guys prepared for it, and how long it took for you to prepare before taking the test. Will 6 months preparation be enough? (I know that may depend on the individual; it's just that I had my Masters over 10 years ago and I haven't studied this much since then). I have Freida L. Carson's 3rd Edition book -- quite daunting to memorize -- but the outline ASCP has provided for study seems to be helpful. Do you have any other book/study aid suggestions? Thank you in advance for your input and advice! C.A. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histogel
Thank you, John!
For some strange reason, the URL in your message got garbled/multiplied, but I
just took this part:
http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf
and that worked.
The enrobing I do is to trap debris on the surface of a coral skeleton at the
interface between "apparently healthy" tissue and "bare" skeleton in studying
the tissue loss diseases of the corals. After solidifying the agarose, I decal
the sample in neutral pH 10% EDTA, then cut ("breadloaf-style") the
agarose-enrobed tissue block into ~2-mm slices and put each in a cassette for
processing. Perhaps my problem is the processing time needs to be increased to
be sure to remove all the water from the agarose and infiltrate it completely.
I don't know what the instructions mean by "non-porous filter paper," either,
has anyone figured this out?
Esther
From: histonet-boun...@lists.utsouthwestern.edu
on behalf of John Kiernan
Sent: Tuesday, January 21, 2014 8:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histogel
The document at
http://lists.utsouthwestern.edu/mailman/listinfo/histonet(http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf";>http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf
gives a thorough description of Histogel, and even says what it is -
hydroxyethyl agarose. In the detailed instructions for various uses, the only
confusing thing is the requirement for "non-porous filter paper"!
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 20/01/14, Elizabeth Chlipala wrote:
> Esther
>
> I agree with Dusko, I fix before I put in histogel and again after the sample
> is placed in histogel, we have no formalin on our tissue processor, we start
> in 50% alcohol. I also process on a longer processing cycle, 1 hour per
> station and similar to Dusko's - denatured ethanol, xylene and paraplast and
> paraplast extra to embed. I've never had a problem (such as overprocessed
> tissue) with the histogel or the sample embedded in the histogel with the
> longer processing cycle. Most of the samples we process are cell blocks or
> tissue fragments such as micronized tissue constructs, which are like powder
> when we receive them.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of dusko trajkovic
> Sent: Monday, January 20, 2014 12:47 PM
> To: Esther C Peters; jennifer.arcand-john...@genzyme.com;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histogel
>
> Esther,
> I mainly process cells, which have been spun down into a small pellet. Also
> mouse DRG's and other very small tissues. I would consider this delicate, so
> do not be afraid to use a longer processing program. Histogel/Agurose is what
> needs longer dehydrating steps.
> We do not use any substitute reagents, so in that aspect I cannot tell you
> how they will affect the processing. Our lab uses ethanol, xylene, and
> Paraplast paraffin. Try a test run and let me know if you were able to get
> successful results.
> Have a good Monday!
> Dusko
>
>
>
> From: Esther C Peters
> To: "jennifer.arcand-john...@genzyme.com"
> ; "histonet@lists.utsouthwestern.edu"
> ; dusko trajkovic
> Sent: Monday, January 20, 2014 11:15 AM
> Subject: RE: [Histonet] Histogel
>
>
> Thank you, Dusko!
>
> I have had the same problem with 1.5% agarose, and I tried starting the
> dehydration with 30% to 50% to 70% ethanol, and using different xylene
> substitutes. It appears that the variable whitening and shrinking happens
> after 100% reagent alcohol and in the xylene substitute (now using
> Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue.
> I guess we'll try this longer processing, but I also work with delicate
> tissues that normally would be a short run (15 min in each reagent). Are your
> tissues thin/delicate/biopsy or cell preps or organ samples? No effect on
> them?
>
> Esther
>
> Esther C. Peters, Ph.D.
> Assistant Professor
> Environmental Science & Policy
> George Mason University
> 4400 University Drive, MS 5F2
> Fairfax, VA 22030-
>
> From: histonet-boun...@li
RE: [Histonet] Histogel
Thank you, Dusko! I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue. I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- From: histonet-boun...@lists.utsouthwestern.edu on behalf of dusko trajkovic Sent: Monday, January 20, 2014 1:58 PM To: jennifer.arcand-john...@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Jennifer, You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. Good Luck. Dusko Trajkovic Pfizer Inc. La Jolla 858-638-6202 From: "jennifer.arcand-john...@genzyme.com" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 20, 2014 7:56 AM Subject: [Histonet] Histogel Dear Histonetters, I have been reading up on the archives for info on Histogel. Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject. Did anyone out there ever figure out how to get consistent results? I have spoken with RA Scientific and they have no additional insights. Here is the background: I have used Histogel for about 4 years now. In September of last year, we started seeing the shriveling Histogel samples. Like others who posted, it was random. I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great. So I have tried many things, always in multiples of 3 or more per condition per run... Fixing in formalin only, embedding in Histogel and storing in PBS until processing Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) Fixing in formalin only, embedding in Histogel and storing in formalin until processing Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). Once processed, there was no rhyme or reason to the results. Some blocks looked great; others within the same group looked shriveled. Sometimes the blocks were white, sometimes they were clear. Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving. That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. Again, no rhyme or reason, some looked good, some looked bad. Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. Yep, you guessed it - no luck. Some looked good, some looked shriveled. So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel! Can anyone out there help me? Thanks, Jenn Jennifer Johnson Staff Scientist Genzyme, a Sanofi Company Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ H
RE: [Histonet] Shipping Slides
The trick to successful shipping is to make sure the slides cannot rattle in the slide box. If you put your tissue, gauze, or bubble wrap layer on top and shut the box, shake gently to see if they can move. If so, you can put a piece of tape across all the slides in the box to anchor them in place (stick the tape ends to the outside of the box. Then make sure the box is securely shut, putting tape or rubber bands around the outside in both directions. Then pack in a well-padded sturdy shipping box! Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Rathborne, Toni [trathbo...@somerset-healthcare.com] Sent: Wednesday, July 31, 2013 11:38 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shipping Slides If the slides are well dried, you can also bunch them together and wrap in a paper towel if you don't have too many. Tape when wrapped, then place in a slide box and insert in a padded envelope for shipping. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Wednesday, July 31, 2013 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shipping Slides Good Morning! Can anyone tell me the best way that you have found to ship slides by Fed Ex? I need to send several cases out and want the safest way possible to eliminate broken slides. We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. Any suggestions would be greatly appreciated! Thank you, Debbie Granato HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraplast Xtra Melting Point?
I have wondered about that myself recently. Noticed lower temperature needed in our waterbaths and melting at lower temperature in embedding center. Shiny drops form on top of our blocks, but not with the tissue. Esthr Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy Biology Program, Medical Technology Coordinator George Mason University From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Marsh, Nannette [n...@stowers.org] Sent: Friday, April 26, 2013 11:29 AM To: 'Rene J Buesa'; Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraplast Xtra Melting Point? On the back of the bag are the instructions : 52 degrees melting point: for best results, use at temperatures above 56 degrees. Would it make a difference if it melted at the lower temperature but, embedding is done at the warmer temperature? Just wondering. Thank you for your response. Nanne Marsh -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 26, 2013 8:52 AM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Xtra Melting Point? The "rule of thumb" is that the lower the paraffin melting point is the higher the compression rate is. It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users. René J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 9:41 AM Subject: [Histonet] Paraplast Xtra Melting Point? Happy Friday to all! For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem. A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point. We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag. Does anyone else have experiences to share? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts. Thank you in advance! Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: interview
In my histology class for (mostly) college seniors, I now try to emphasize learning those specific qualities (follow directions, pay close attention to detail, and communicate well), as well as the subject. It is amazing how often, when you specify to "label only 4 structures" or "provide 2 complete sentences to explain" or "follow this format in a report," students will not do this! Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030- - Original Message - From: "Kienitz, Kari" Date: Tuesday, January 8, 2013 4:10 pm Subject: [Histonet] RE: interview > Hi Gale, > > In my career I have had the pleasure of hiring many OJT > histotechs. With varying degrees of education and a whole range > of personalities. The things that tend to really stand out in the > candidates who excelled are the ones who could follow direction, > pay close attention to detail, and communicate well. Histology is > a field where as many mundane tasks must be done as the > complicated ones. > > For many years we would administer to all final candidates a > simple aptitude test. It was honestly something most 3rd graders > could do. Its was amazing how many people would get caught up > following directions on where to place their name on the paper and > simple math. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Portland Gastroenterology > The Oregon Clinic > NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkien...@orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for > the exclusive and confidential use of the intended recipient. If > you are not the intended recipient, please do not read, distribute > or take action in reliance upon this missive. If you have received > this in error, please notify the sender immediately by reply e- > mail and delete this message and its attachments from your > computer system. Thank you > > From: histonet-boun...@lists.utsouthwestern.edu [histonet- > boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron > [ga...@unionhospital.org]Sent: Tuesday, January 08, 2013 10:02 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interview > > Hello, > I just found out today I will be doing 2nd interviews for 3 > candidates for a part time Histology position at our hospital on > Friday of this week. These candidates are not histotechs but are > willing to do online training and take ASCP board exam within 24 > months. I would appreciate some help with what questions to ask. I > did not attend the 1st interviews but these were done by our lab > manager who does not know a lot about what we do I > histology.Thank you! > > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it > is addressed and may contain information that is privileged, > confidential or otherwise protected from disclosure. > Dissemination, distribution or copying of this e-mail or the > information herein by anyone other than the intended recipient, or > an employee or agent responsible for delivering the message to the > intended recipient, is prohibited. If you received this message in > error, please delete without copying and kindly e-mail a reply to > inform us of the mistake in > delivery.___Histonet > mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Acid-cleaning of slides for metals stains
We are going to do special stains for iron (Perl's Prussian Blue for ferric iron, Mallory's Method from WebPath: Internet Pathology Laboratory) and copper (Rhodanine from Carson and Hladik) in mouse livers. We have on hand unopened boxes of plus-charged microscope slides. Do we need to acid clean these slides before we put sections on them for these stains (understanding that the charge will no longer exist, but the cleaning is more important for these stains)? I would also appreciate any insights about the best acid-cleaning procedure for all glassware for these stains. I have used nitric acid in the past, swirling it around the staining dishes and covering glass racks in a staining dish (how long should this be for?), then rinsing with double-deionized water (or would you recommend distilled only?). Thank you! Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030- Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epete...@gmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Scope of Practice
Hi Bharti,
As Rene notes, it is "not a given" that every researcher in conservation or
environmental science uses histology in their research, although they should!
But, everyone is specialized these days and we can only know and apply so much;
however, collaborations with histologists and histopathologists do occur, and
many of us have this expertise and use histotechniques in our research.
In addition to using histology to study reproduction and development,
researchers use it in taxonomy and systematics, physiology, biomarkers,
toxicology, and molecular biology studies (of particular value is laser-capture
microdissection to obtain targeted cells from FFPE or frozen sections to
extract DNA and learn about the genetics of abnormal cells (tumors),
microorganisms, or parasites. A recent development in conservation is
conservation medicine. Scientists and veterinarians are studying how diseases
and anthropogenic changes in the environment are affecting biodiversity and
habitats around the world. This requires use of histology to help diagnose
diseases and identify etiologic agents, with the hope of managing conservation
programs to improve the organisms' and humans' health ("one health").
In the Bay area, check out the University of California at Davis' School of
Veterinary Medicine. They have a histology facility. Let me know if you need a
contact there (or maybe someone from it will reply to Histonet!).
Esther C. Peters, Ph.D.
Assistant Professor
Department of Environmental Science & Policy
Biology Program/Medical Technology Coordinator
George Mason University
4400 University Drive, MSN 5F2
Fairfax, VA 22030-
Office: David King Hall 3057
Phone: 703-993-3462
Fax: 703-993-1066
epete...@gmu.edu
- Original Message -
From: Rene J Buesa
Date: Sunday, February 26, 2012 5:09 pm
Subject: Re: [Histonet] Scope of Practice
> As you point out, histology is a fundamental tool in human AND
> veterinary pathology and every clinical AND veterinary laboratory
> performs histology procedures/tests routinely.
> In other fields of biology either animal or plant biology it is
> NOT customary to do histology studies of plants or animals UNLESS
> the researcher wants to study a specific aspect of the biology of
> the subject s/he is studying.
> In this sense it is frequent to study reproduction histology or
> whole histology of small animals or developmental steps (ontogeny).
> Other than that it is NOT a given that "natural studies/biology"
> include histology work, and they are the exception, not the rule.
> Outside human or veterinary pathology, histology work is not frequent.
> René J.
>
> --- On Sun, 2/26/12, Bharti Parihar wrote:
>
>
> From: Bharti Parihar
> Subject: [Histonet] Scope of Practice
> To: histonet@lists.utsouthwestern.edu
> Date: Sunday, February 26, 2012, 4:52 PM
>
>
> Hello everyone! I am currently enrolled in an HT program through
> IUPUI and
> am starting to send resumes out as I encroach into the end of the
> program.I asked my supervisor recently if there was a scope for
> this field in the
> world of conservation?! She wasn't sure but recommended this site
> to me to
> try to find any answers. It seems as though while
> biologists/zoologistsetc. are out there on the field studying the
> respective life form they do,
> there would be a need for histology in there somewhere, but I
> don't really
> know how that works in that realm. I'm only familiar with Clinical
> Labssince that's all I've ever worked in. Any thoughts/ direction?
> I'd like to
> get involved in that if possible. I'm looking for work in Calfornia,
> specifically the bay area if possible. But as long as I can take
> the BART
> to where it is that's fine with me also. I wanna make driving to
> work the
> last resort, but ya know, gotta work and pay the bills so, if I
> have to
> drive, so be it. Thanks ahead of time to those that can shed light
> on this
> subject matter for me!!!
> Sincerely,
> Bharti Parihar
> ___
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>
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[Histonet] Senior Research Assistant Position in Histology
Please see the following job announcement for a Senior Research Assistant at George Washington University in Washington, DC. Contact Dr. Patricia Latham at GWU if you are interested in this position: plat...@mfa.gwu.edu Full-time Research Assistant - Histotechnologist A position is available for a histotechnologist in a new research core laboratory in the GWU School of Biomedical Sciences. This person will be expected to maintain a histology laboratory, including ordering of supplies, washing glassware and maintaining equipment for optimal function, daily logs with inventory of specimens and blocks, labeling of samples and slides, tissue processing and embedding, microtome and cryostat sections, routine stains, special stains and immunostains with appropriate controls, as required to meet the needs of researchers at GWU. This person will need to work independently to deliver excellent quality results in a timely manner. The person should have strong communication skills and managerial skills, since there will be a need to work with researchers in several labs and to maintain inventory from several sources, to keep track of the flow of specimens and charges. The position will require data entry into an audit-trail database and the mainten ance of meticulous records, with an exchange of data to the research center off-site and to labs at international sites. This person should have some experience in laboratory research, implementation of new techniques and the ability to trouble-shoot problems that might arise in this setting. Ideally, the person will have an entreprenurial spirit, since the success of the lab will determine its future existence. The full-time position is assured for 2 years, but it can be extended and/ or grow to full-time, if the lab is successful. Supervision: The work will be supervised by a Pathologist as Project Manager. Entry level qualifications: • Bachelor’s degree in a clinical science with a course in Histology or equivalent combination of training and experience. • Experience working in a research setting. • Favored is eligibility or certification as a Histotechnologist or Histotechnician by the American Society for Clinical Pathology. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030- Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epete...@gmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blades
We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that is clearly marked "Microtome Blades for Facing Blocks" to be used another day. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030- Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epete...@gmu.edu - Original Message - From: "Webb, Dorothy L" Date: Friday, June 24, 2011 4:53 pm Subject: [Histonet] blades > Trying to clean up some things hanging out there in our lab and > wondering what everyone does with a blade that has been used > minimally and tech done for the day with the microtome. Where do > you store that blade for use tomorrow or do you toss and not worry > about the cost involved? I do not like them sitting on top of the > microtome. Any good ideas?? Thanks, as always! > > > > > This e-mail and any files transmitted with it are confidential and > are intended solely for the use of the individual or entity to > whom they are addressed. If you are not the intended recipient or > the individual responsible for delivering the e-mail to the > intended recipient, please be advised that you have received this > e-mail in error and that any use, dissemination, forwarding, > printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately > notify the HealthPartners Support Center by telephone at (952) 967- > 6600. You will be reimbursed for reasonable costs incurred in > notifying us. HealthPartners R001.0 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
