To whom it may concern, I am a undergraduate researcher at Vassar College. I am performing immunohistochemistry on mice using Arc primary, anti-mouse biotinylated IgG (1:200),ABC (Vector labs) and DAB. So far we have gotten really great staining, however the last immuno I ran, the sections, once in DAB were sticking to the bottom of the wells. Does anyone have any idea as to why this would happen? Thank you!
Best, Gabrielle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
