[Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] help (UNCLASSIFIED)
Classification: UNCLASSIFIED
Caveats: NONE
Patsy,
I have removed Silver Nitrate on my hands by first treating the stain with
Iodine solution then flushing the area with Sodium Thiosulfate solution (5% ?)
then washing my hands thoroughly with soap and water. It removed the Silver
Nitrate. This was a trick that one of my instructors used while I was in
school. She got the idea from one of the silver stains that we were doing
(Reticulin, I think). I don't know if how it will work on your face or if it
will irritate the delicate skin but I think that I would try it anyway just to
get rid of the black mark.
Thanks for the laugh and my heart goes out to you.
Diane G.
Diane C. Gladney, HT (ASCP)
Supervisor, Anatomical Pathology
Moncrief Army Community Hospital
Dept. of Pathology
4500 Stuart St.
FT. Jackson, SC 29207
Email: diane.glad...@amedd.army.mil
Phone: 803-751- 2530
FAX: 803-751-7829
DSN: 734-2530
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, March 23, 2010 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help
After you stop laughing seriously I need some help here, apparently I got my
fingers in some silver nitrate yesterday and touched my face under my nose
over my lip and now I have black spots that won't come off. I have done
this on my hands before but never on my face. So far I have tried soaking a
cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried
putting some gold chloride on it to see if I could tone it down, to no
avail. Any ideas? Make up only goes so far and lasts so long.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
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RE: [Histonet] Ink issues (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE We use Distilled White Vinegar. I buy it at the grocery store by the gallon. It is the acetic acid in bouin's solution that fixes the ink to the tissue, not the picric acid. I have been using white vinegar for more years than I care to remember. Hope this helps. Diane Gladney Supervisor, Histology Dept. Moncrief Army Community Hospital Ft. Jackson, SC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to stick to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a ink stay acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to fix your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP ? (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE We have a MSDS for all of our chemicals. The information is listed in the MSDS. We keep copies in our MSDS Books in the lab for fast and easy access. The MSDS's are updated yearly, when we receive new chemicals or the manufacturer sends us their latest MSDS on their products. This use of the MSDS and the information listed is addressed in our Safety Standard Operating Procedures (SOP). We also list in this SOP all known carcinogens, reproductive toxicity and acute toxicity used in the lab. They are to be handled in accordance with the recommendations of the MSDS. Thanks, Diane G. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.glad...@amedd.army.mil Phone: 803-751- 2530 FAX: 803-751-7829 DSN: 734-2530 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Monday, August 10, 2009 10:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP ? How does one gather this information in the lab? What are your steps? GEN.70550 Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specific handling requirements for these chemicals - Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Xylene substitutes (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon Xylene Substitute (formally known as Histosolv). I have used this for many years with excellent results. I have never had any problems using this in the processor. We had 2 very old VIP tissue processors before we got the Leica processors and used Histosolv in those processors also. I have never heard of Sub-X. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.glad...@amedd.army.mil Phone: 803-751- 2530 FAX: 803-751-7829 DSN: 734-2530 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, August 05, 2009 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitutes William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
