[Histonet] RE:Formaldehyde

2009-12-03 Thread Griffin, Michelle A
Our lab tried the self contained grossing station with filters
(bench-top) and we had troubles with it adequately filtering formalin
fumes (even though the spec sheet stated that was what it was for...from
MOPEC). We ended up installing a fume hood to use for gross prosection,
waste discard, etc. In our situation and experience, the fume hood was
the best way to go, especially when we need to use extra precautions for
tissue fixing/staining/etc. 

Michelle A. Griffin
Research Assistant II
University of Iowa
Department of Pathology
200 Hawkins Drive
143 Medical Research Center
Iowa City, IA 52242


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Thursday, December 03, 2009 11:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FORMALDEHYDE

Good Morning Histo Land, I was just informed that on my last test sample
for formaldehyde vapors was very high.  Right now I have to wear a
respirator while grossing and assisting grossing.  We are looking at why
the Mopec grossing station is not working in fact we have not had really
good results since we put it in.  I'm wondering if anyone else is having
the same problem or know of a better solution.  Our grossing station is
the one that is self contained with filters. Also is anyone using with
good result a formalin substitute? If so which one? 
Thanks and Have a great day,

Kathy Gorham, H.T.
Grande Ronde Hospital
La Grande Oregon


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[Histonet] Whole mount porcine trachea staining

2009-11-05 Thread Griffin, Michelle A
Would anyone have an idea about how to stain whole porcine trachea so
that the cartilage rings show prominently? There have been several
excellent articles that use Alcian Blue or an Alcian Blue/Alizarin Red S
combination to stain rodent tracheas (mouse/rat) and the pictures are
wonderful (Schipani et al., 2001; Regnier et al., 2002; Dolle et al.,
1993). However, I have tried to reproduce the results using the same
protocols just a different species and am having no luck (I did try out
each method with some mouse tracheas in my lab and the results mimicked
what I saw in published articles). I have tried different
digestion/clearing solutions, times, pH, etc and continue to end up with
a trachea that is quite ugly with no differentiation between cartilage
rings and connective tissue. 

Does anyone have a suggestion on why pig trachea would not stain even
similar to mouse trachea? Any suggestions would be greatly appreciated.
Thanks so much for your time and input. 

Michelle A. Griffin BA, BS, MHA
University of Iowa
Comparative Pathology Laboratory
200 Hawkins Drive
143 Medical Research Center
Iowa City, IA 52242
319-384-4620
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