[Histonet] Histology in the Caribbean

2010-08-22 Thread Histonet Alias
I am looking for any information on labs or hospitals that do histology in
the Caribbean. Possibly the Turks and Caicos Islands, Bahamas, or USVI.

Thanks!

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Histology Manager
Pathology Laboratory
United States
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Re: [Histonet] Hypothetical question

2010-02-16 Thread Histonet Alias
Ha Ha Kim! Are you recruiting? I think if you are used to cutting 50 blocks
then 100 will be too much. Most tech are used to their certain workload
and if it increases or if they go to a bigger lab it will take some
adjustment.

On Fri, Feb 12, 2010 at 12:32 PM, kim.dona...@bhcpns.org wrote:

 Out of curiosity, how many Histotechs would like to work in a lab where
 you have an average of 100-150 blocks per day, a hand full of special
 stains at most which are done on the Dako artisan, IHC done on the Ventana
 XT and less than a hand full of cytology preps. You might have 0-5 frozen
 sections per day as well
 .
 You would even have help from one or two other technologist. You could
 work 4/10 hour days or 5/8 hour days.

 Do any of you think this sounds like too much work?

 Maybe this hypothetical lab is located in the beautiful area of Pensacola
 Fl?

 All of this is Hypothetical... 

 Again, I'm just curious :-)

 Thanks



 Kim Donadio
 Pathology Supervisor
 Baptist Hospital
 1000 W Moreno St.
 Pensacola FL 32501
 Phone (850) 469-7718
 Fax (850) 434-4996

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[Histonet] Michelle Rederick where did you go?

2010-02-16 Thread Histonet Alias
*Does anyone have Michelle Rederick's contact info? She was working for
Milestone.*



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United States
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[Histonet] Negative Staining Positive

2009-08-13 Thread Histonet Alias
Has anyone noticed their negative mouse having some positive staining using
the Ventana Benchmark XT? We use the iview detection. Any ideas? Thank you.

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Histology Manager
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United States
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Re: [Histonet] Destain Hematoxylon

2009-05-04 Thread Histonet Alias
Acid alcohol. It may not remove all of it but it will remove the majority.

On Mon, May 4, 2009 at 5:32 AM, V. Neubert histonet.nos...@vneubert.comwrote:

 Hi,

 I want to destain hematoxylin (Shandon's Gill II). How to do that?

 Thanks in advance,

 V. Neubert

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Re: [Histonet] grossing

2009-03-16 Thread Histonet Alias
Define presence? If they are able to have access a technical supervisor or a
Pathologist if they have a question then this may just cover it. We have a
Pathologist on call that can be in within minutes if there are any
questions.

On Mon, Mar 16, 2009 at 10:55 AM, godsgal...@aol.com wrote:

 I hate to beat a dead horse.but how do you handle non-certified
 grossing staff.? They all meet the CLIA guidelines, but are they allowed to
 gross in the evening without the presence of a technical supervisor?

 And how do you handle salaries for those that process and those that
 grossaccording to the CAP guidelines?


 Thanks,
 Roxanne
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Re: [Histonet] 7B11 clone of Ki67

2008-12-12 Thread Histonet Alias
Why not use the (30-9) clone? It is much easier to come by. I sue it on my
Benchmark, cat# 790-4286.

On Fri, Dec 12, 2008 at 1:45 PM, Sebree Linda A lseb...@uwhealth.orgwrote:

 Good afternoon, 'netters,

 I am in desperate need of Ki67 antibody, clone 7B11 preferably, to use
 on Ventana instruments.  I didn't realize we were running low and our
 usual vendor has no stock.  I'd prefer a predilute but only if it is
 known to work on our instruments.  I don't have the time to search this
 out myself so I am turning to all of you.

 Thanks,
 Linda

 Linda A. Sebree
 University of Wisconsin Hospital  Clinics
 IHC/ISH Laboratory
 DB1-223 VAH
 600 Highland Ave.
 Madison, WI 53792
 (608)265-6596


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United States
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Re: [Histonet] Paraffin wax

2008-12-11 Thread Histonet Alias
I have had very good luck with Paraplast Plus for infiltration and using the
Paraplast Xtra for embedding.

On Thu, Dec 11, 2008 at 10:00 AM, Charles, Roger [EMAIL PROTECTED]wrote:

 Hello,
 Could someone share their knowledge of the Paraplast line of paraffin with
 me?  We are being forced to change from the TissuePrep line.
 Thanks

 Roger Charles
 Microbiologist
 Pennsylvania Veterinary Laboratory
 2305 N Cameron St
 Harrisburg, PA 17110
 717-787-8808

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Re: [Histonet] microwave processor

2008-12-08 Thread Histonet Alias
The Pathos can only run one run at a time. It does everything a conventional
processor does. It is fully automated.

On Mon, Dec 8, 2008 at 7:50 AM, Evans, Andria B. [EMAIL PROTECTED]wrote:

 We are currently looking at getting another microwave processor.  We
 currently have the Histos 5 and are looking at getting another one of those
 or a Pathos (the big guy from milestone).  But we have little information on
 the Pathos.  I have a couple questions that maybe someone could answer

 Can you run more then one run at a time on the Pathos? (currently we can
 run 2 runs at a time on the Histos 5)
 Does the Pathos do the everything on the machine from fixation, to alcohol
 rinses, to the end product?? (We have to do all the rinses and changes
 manually with the Histos 5)

 If anyone has any other information on the microwave processors that they
 would like to share that would be great!!

 Thanks

 Andria B Evans, HTL(ASCP)CM


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United States
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Re: [Histonet] Ventana Benchmark XTs

2008-12-03 Thread Histonet Alias
Let's agree to disagree here. I will continue to post when I feel necessary
and the people who don't agree with my reasoning can just ignore me. Just
for the record I do not work for any vendor and I am a Histology Manager for
a private laboratory in the midwest. Will my name make any difference in my
posts? Take that for what it is worth. This thread was really ignited by me
giving an opinion about a product or company that many people do not care
for. If you don't like it don't use it. You can give your opinion about it
when asked just like I can. I will use Joe the toes suggestion and change up
my signature to avoid any further issue. I work for one of the larger lab
companies that do not want their name or employees attached to any opinion
other than the companies. If you can not understand that then I can't help
you. I have mouths to feed and I do not have the luxury of changing jobs and
moving to a new location. Do I like hiding my identity? No. I have been on
here for years with an open identity. I have found a way to still contribute
and gain knowledge from this listserv and a few of you are all about status
and credibility. SO let us just end this thread and save space for real
Histology issues.
Al Ias HT(ASCP)
Histology Manager
A MidWest Lab


On Tue, Dec 2, 2008 at 7:33 PM, Joe Nocito [EMAIL PROTECTED] wrote:

 I have to agree with Al ( can I call you Al, Alias?) on this one. Remember
 when I had to sign off of the Histonet a couple of years ago because a
 certain company contacted my CEO and filed a complaint. I almost lost my job
 because I posted an honest opinion. An opinion that was greatly shared if I
 remember correctly. In this era of financial uncertainties, foreclosures and
 all, I don't blame Al. I'm sure he/she has bills just the rest of us do.
 Bills that won't go away if he/she loses the job.
 That's my story and I'm sticking to it.

 Joe Nocito BS, PA, HT(ASCP)QIHC
 San Antonio, TX

 - Original Message - From: Histonet Alias 
 [EMAIL PROTECTED]
 To: Della Speranza, Vinnie [EMAIL PROTECTED]
 Cc: histonet@lists.utsouthwestern.edu; Tracey Lenek 
 [EMAIL PROTECTED]; Burton,Lynn [EMAIL PROTECTED]; Joanna
 Bartczak-McKay [EMAIL PROTECTED]; Martin Trotter 
 [EMAIL PROTECTED]
 Sent: Tuesday, December 02, 2008 1:19 PM

 Subject: Re: [Histonet] Ventana Benchmark XTs


  Mr. Della Speranza, I respect what you have done for the histology
 community
 and we have had pleasant conversations in the past but you can not speak
 for
 the Histonet members. You act as if I am hiding my identity to create
 havoc
 and disruption. I have not done this nor will I do this. I have been on
 histonet for many years and I will continue to do so. As I explained in my
 previous posts my current employer does not want any opinions, help, or
 questions associated with the laboratory. That is the only reason that I
 have chosen to remain anonymous. Would it make anyone feel better if I
 made
 up a fake name and lab as my signature?  I will continue to post and speak
 freely without breaking any rules of this listserver. If I respond or ask
 a
 question, those who find my years of experience not credible then
 I apologize.

 On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie [EMAIL PROTECTED]
 wrote:

  I'm afraid your opinion will not be credible as long as you insist on
 remaining anonymous. It has nothing to do with whether you like or
 dislike a
 particular product or instrument.  Histonet members appreciate the
 responsibility that comes with posting one's views or knowledge by
 identifying themselves accordingly. In my opinion anyone who insists on
 remaining anonymous should not be permitted to participate on the list.


 Vinnie Della Speranza
 Manager for Anatomic Pathology Services
 Medical University of South Carolina
 165 Ashley Avenue  Suite 309
 Charleston, South Carolina 29425
 Tel: (843) 792-6353
 Fax: (843) 792-8974


 -Original Message-
 From: [EMAIL PROTECTED] [mailto:
 [EMAIL PROTECTED] On Behalf Of Histonet Alias
 Sent: Saturday, November 29, 2008 11:03 AM
 To: Mark Tarango
 Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
 Burton, Lynn; Joanna Bartczak-McKay
 Subject: Re: [Histonet] Ventana Benchmark XTs

 Sorry I don't work for Ventana and I have no beef with them. It
 may disappoint some that I have not had a bad experience. So my opinion
 does
 not count because I have had a different experience with my XT than you?
 I
 use the name of The Unknown HT so I can express my opinions freely
 without
 my employer receiving phone calls because certain people don't like my
 opinions.

 On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango [EMAIL PROTECTED]
 wrote:

  I don't know how much the opinion of The Unknown HT(ASCP) counts. 
 You
  aren't with Ventana I hope.
 
  Mark
 
  On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias 
 [EMAIL PROTECTED]wrote:
 
  They are switching to a more reliable manufacturer. They are being
  proactive
  and switching them before

Re: [Histonet] Blue haze

2008-12-03 Thread Histonet Alias
On Wed, Dec 3, 2008 at 12:47 PM, Marshall, Kimberly 
[EMAIL PROTECTED] wrote:

 Howdy all,

   I have just changed over to Surgi Path's H  E system.  With the latest
 fear of running out of hematoxylin and all my Pathologist wanted us to
 change over.  Its a great stain but we have a bad haze  in the background.
  It is not hurting the tissue or DX at all, but to ME it looks awful.  Is
 there anyone else out there using this?   We are not using positive charged
 slides or anything added to my water bath.  Any suggestions  Thanks in
 advance

 Kimberly Marshall HT (ASCP)


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United States
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Re: [Histonet] Ventana Benchmark XTs

2008-12-02 Thread Histonet Alias
Mr. Della Speranza, I respect what you have done for the histology community
and we have had pleasant conversations in the past but you can not speak for
the Histonet members. You act as if I am hiding my identity to create havoc
and disruption. I have not done this nor will I do this. I have been on
histonet for many years and I will continue to do so. As I explained in my
previous posts my current employer does not want any opinions, help, or
questions associated with the laboratory. That is the only reason that I
have chosen to remain anonymous. Would it make anyone feel better if I made
up a fake name and lab as my signature?  I will continue to post and speak
freely without breaking any rules of this listserver. If I respond or ask a
question, those who find my years of experience not credible then
I apologize.

On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie [EMAIL 
PROTECTED]wrote:

 I'm afraid your opinion will not be credible as long as you insist on
 remaining anonymous. It has nothing to do with whether you like or dislike a
 particular product or instrument.  Histonet members appreciate the
 responsibility that comes with posting one's views or knowledge by
 identifying themselves accordingly. In my opinion anyone who insists on
 remaining anonymous should not be permitted to participate on the list.


 Vinnie Della Speranza
 Manager for Anatomic Pathology Services
 Medical University of South Carolina
 165 Ashley Avenue  Suite 309
 Charleston, South Carolina 29425
 Tel: (843) 792-6353
 Fax: (843) 792-8974


 -Original Message-
 From: [EMAIL PROTECTED] [mailto:
 [EMAIL PROTECTED] On Behalf Of Histonet Alias
 Sent: Saturday, November 29, 2008 11:03 AM
 To: Mark Tarango
 Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
 Burton, Lynn; Joanna Bartczak-McKay
 Subject: Re: [Histonet] Ventana Benchmark XTs

 Sorry I don't work for Ventana and I have no beef with them. It
 may disappoint some that I have not had a bad experience. So my opinion
 does
 not count because I have had a different experience with my XT than you? I
 use the name of The Unknown HT so I can express my opinions freely without
 my employer receiving phone calls because certain people don't like my
 opinions.

 On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango [EMAIL PROTECTED]
 wrote:

  I don't know how much the opinion of The Unknown HT(ASCP) counts.  You
  aren't with Ventana I hope.
 
  Mark
 
  On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias 
 [EMAIL PROTECTED]wrote:
 
  They are switching to a more reliable manufacturer. They are being
  proactive
  and switching them before they fail down the road. My XT has been a
  workhorse but we know certain peoples opinions about the company on
 here.
  I
  will take it any day over the rest.
 
  On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn [EMAIL PROTECTED]
  wrote:
 
   We have had some trouble but they are planning to replace them. We
  received
   a letter that they had changed vendors for parts and found they need
 to
   switch back. They did replace one section earlier when it stopped
  working
   completely.
  
   Lynn Burton
   Lab Assoc. I
   Animal Disease Lab
   Galesburg, Il 61401
  
   
  
   From: [EMAIL PROTECTED] on behalf of Tracey
  Lenek
   Sent: Wed 11/26/2008 10:59 AM
   To: histonet@lists.utsouthwestern.edu
   Cc: Martin Trotter; Joanna Bartczak-McKay
   Subject: [Histonet] Ventana Benchmark XTs
  
  
  
   Hi,
  
   We have had on-going thermal slide pad issues with our XTs since they
  were
   installed
   in February. The slide tray assemblies have been replaced not once but
   twice and we are still having inconsistent
   results with the temp verifier slides.  Has anyone experienced the
 same
   issues with this
   instrumentation?
  
   Thanks
   Tracey Lenek
   Tech III - Anatomic Pathology
   Calgary Laboratory Services
   403-770-3588
  
   
   This message and any attached documents are only for the use of the
   intended recipient(s), are confidential and may contain privileged
   information. Any unauthorized review, use, retransmission, or other
   disclosure is strictly prohibited. If you have received this message
 in
   error, please notify the sender immediately, and then delete the
  original
   message. Thank you.
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Re: [Histonet] Expiration date checks

2008-12-02 Thread Histonet Alias
I actually have an excel spread sheet of expiration dates. I also place
those dates into the outlook calender and I receive a notification a week
before the expiration.

On Mon, Dec 1, 2008 at 6:49 PM, Patti Loykasek [EMAIL PROTECTED]wrote:

 Hi all. I am hoping for some clever ideas on the best method to check for
 expired reagents. We all know that we shouldn't use them, I'm sure we all
 actively monitor this (right)! I must admit we occasionally have expired
 reagents - a stain or buffer we haven't used in a while is in a cupboard or
 fridge  it has expired. What do others do to monitor  prevent this from
 happening? We have a weekly safety check that spot checks a certain # of
 reagents for expiration, but we probably have thousands of reagents  don't
 check every single bottle every week. Thanks for the input.

 Patti Loykasek



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Re: [Histonet] Ventana Benchmark XTs

2008-11-29 Thread Histonet Alias
Sorry I don't work for Ventana and I have no beef with them. It
may disappoint some that I have not had a bad experience. So my opinion does
not count because I have had a different experience with my XT than you? I
use the name of The Unknown HT so I can express my opinions freely without
my employer receiving phone calls because certain people don't like my
opinions.

On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango [EMAIL PROTECTED] wrote:

 I don't know how much the opinion of The Unknown HT(ASCP) counts.  You
 aren't with Ventana I hope.

 Mark

 On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias [EMAIL PROTECTED]wrote:

 They are switching to a more reliable manufacturer. They are being
 proactive
 and switching them before they fail down the road. My XT has been a
 workhorse but we know certain peoples opinions about the company on here.
 I
 will take it any day over the rest.

 On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn [EMAIL PROTECTED]
 wrote:

  We have had some trouble but they are planning to replace them. We
 received
  a letter that they had changed vendors for parts and found they need to
  switch back. They did replace one section earlier when it stopped
 working
  completely.
 
  Lynn Burton
  Lab Assoc. I
  Animal Disease Lab
  Galesburg, Il 61401
 
  
 
  From: [EMAIL PROTECTED] on behalf of Tracey
 Lenek
  Sent: Wed 11/26/2008 10:59 AM
  To: histonet@lists.utsouthwestern.edu
  Cc: Martin Trotter; Joanna Bartczak-McKay
  Subject: [Histonet] Ventana Benchmark XTs
 
 
 
  Hi,
 
  We have had on-going thermal slide pad issues with our XTs since they
 were
  installed
  in February. The slide tray assemblies have been replaced not once but
  twice and we are still having inconsistent
  results with the temp verifier slides.  Has anyone experienced the same
  issues with this
  instrumentation?
 
  Thanks
  Tracey Lenek
  Tech III - Anatomic Pathology
  Calgary Laboratory Services
  403-770-3588
 
  
  This message and any attached documents are only for the use of the
  intended recipient(s), are confidential and may contain privileged
  information. Any unauthorized review, use, retransmission, or other
  disclosure is strictly prohibited. If you have received this message in
  error, please notify the sender immediately, and then delete the
 original
  message. Thank you.
  ___
  Histonet mailing list
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  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
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Re: [Histonet] Help with protocols

2008-11-28 Thread Histonet Alias
If the CC1 standard 32 with amp, 42 degrees does not work then try using
protease 1 4-6 minutes on it.

On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer [EMAIL PROTECTED]wrote:

 Hi All:

 I have been working up the PMS2 and MLH2 mismatched repair genes and I am
 not satisfied with my results.  I am using the Cell Marque antibodies and
 the Ventana XT using CC1, mild, standard and I have tried the antibodies
 with block and amplification, and antibody times from 32 min- 1 hour and
 the
 stain is not satisfactory.  Anyone who has these antibodies in their lab
 and
 would like to share your  protocols,  I would be grateful for your help.
 The MSH2 and MSH6 are working great.

 Lena Spencer

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Re: [Histonet] Ventana Benchmark XTs

2008-11-28 Thread Histonet Alias
They are switching to a more reliable manufacturer. They are being proactive
and switching them before they fail down the road. My XT has been a
workhorse but we know certain peoples opinions about the company on here. I
will take it any day over the rest.

On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn [EMAIL PROTECTED]wrote:

 We have had some trouble but they are planning to replace them. We received
 a letter that they had changed vendors for parts and found they need to
 switch back. They did replace one section earlier when it stopped working
 completely.

 Lynn Burton
 Lab Assoc. I
 Animal Disease Lab
 Galesburg, Il 61401

 

 From: [EMAIL PROTECTED] on behalf of Tracey Lenek
 Sent: Wed 11/26/2008 10:59 AM
 To: histonet@lists.utsouthwestern.edu
 Cc: Martin Trotter; Joanna Bartczak-McKay
 Subject: [Histonet] Ventana Benchmark XTs



 Hi,

 We have had on-going thermal slide pad issues with our XTs since they were
 installed
 in February. The slide tray assemblies have been replaced not once but
 twice and we are still having inconsistent
 results with the temp verifier slides.  Has anyone experienced the same
 issues with this
 instrumentation?

 Thanks
 Tracey Lenek
 Tech III - Anatomic Pathology
 Calgary Laboratory Services
 403-770-3588

 
 This message and any attached documents are only for the use of the
 intended recipient(s), are confidential and may contain privileged
 information. Any unauthorized review, use, retransmission, or other
 disclosure is strictly prohibited. If you have received this message in
 error, please notify the sender immediately, and then delete the original
 message. Thank you.
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Re: [Histonet] RE: Floaters

2008-11-24 Thread Histonet Alias
ANP.23350 Phase II
N/A   YES   NO



*Are flotation baths clean and well-maintained, and is there a procedure for
preventing cross-contamination of paraffin sections in the bath?*

* *

NOTE:  Of particular importance are periodic water changes or blotting of
the water surface so that sections from one patient block are not
inadvertently carried over to another case (so-called floaters or
extraneous tissue).



COMMENTARY:

On Mon, Nov 24, 2008 at 4:44 AM, Edwards, R.E. [EMAIL PROTECTED] wrote:

 I tend to  try and  poke them down with a metal coat hanger!.

 -Original Message-
 From: [EMAIL PROTECTED] [mailto:
 [EMAIL PROTECTED] On Behalf Of
 [EMAIL PROTECTED]
 Sent: 21 November 2008 18:17
 To: [EMAIL PROTECTED]
 Subject: [Histonet] Floaters

 Would anyone care to share their policy on floaters? Also, does anyone
 know if there is a CAP policy in floaters?

 Teresa Hallada BS, MT/CT (ASCP)
 Lead Cytotechnologist
 MidMichigan Health - Gratiot
 [EMAIL PROTECTED]
 989.463.1101 ext 3423

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Re: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question

2008-11-18 Thread Histonet Alias
I use it as a mixer!

On Tue, Nov 18, 2008 at 1:16 PM, Cheryl [EMAIL PROTECTED] wrote:

 Read down to Characteristics-- I want to know who they got to taste this
 stuff??!!!

 Cheryl
 _

 HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants.

 SYNONYMS
 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene,
 Safsolv, Histolene, dipentene.
 GENERAL PRECAUTIONS
 Avoid skin contact, can cause dermatitis.
 Avoid inhalation.
 Keep away from heat or naked flames.
 Wash hands thoroughly after handling.
 CHARACTERISTICS
 Clear liquid of low viscosity with a fresh, light and sweet citrusy odour.
 The taste is sweet, refreshing, citrus-like and mild.




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Re: [Histonet] Dako Link True Positive ID

2008-11-16 Thread Histonet Alias
Have you looked into the Ventana Vantage system yet? I saw a demo of this
and it looks pretty impressive.

On Fri, Nov 14, 2008 at 3:56 PM, Sharon Scalise 
[EMAIL PROTECTED] wrote:

 We are investigating systems for specimen tracking using bar-code
 technology (including cassette and slide printers).  Does anyone have any
 experience with the Dako Link True Positive ID system?  Apparently it is not
 out in the U.S. as much as in Europe (according to Dako).  Any information
 would be great (on the Dako system or any others out there).

 Have a great Week-End!


 Sharon E. Scalise, HTL (ASCP)
 Histology Supervisor
 William Beaumont Hospital
 Royal Oak, MI 48073
 248 898-5981
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