[Histonet] Fw: orders from resident surgeons

[Horn, Hazel V via Histonet]

I was not clear enough in my question.  I'm not speaking of pathology residents 
ordering tests.  I'm asking if you allow general resident physicians to order 
pathology tests.  Such as gross and micro.

We are a teaching hospital and have never allowed a resident to order pathology 
if they aren't a staff physician (surgeon).

Thanks!



From: Horn, Hazel V
Sent: Thursday, April 13, 2017 8:17 AM
To: histonet
Subject: orders


In the 25+ years I have worked at ACH we have never allowed a resident 
physician to order a pathology test.  I am now being asked why we do this?   I 
have no idea if there is a rule from Medicaid or insurance providers that all 
orders must be from a staff physician.   Does your hospital allow residents to 
place pathology orders?



Hazel Horn

Supervisor of Histology/Autopsy/Transcription

Anatomic Pathology

Arkansas Children's Hospital

1 Children’s Way | Slot 820| Little Rock, AR 72202

501.364.4240 direct | 501.364.1241 fax

hor...@archildrens.org<mailto:hor...@archildrens.org>

archildrens.org<http://www.archildrens.org/>

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[Histonet] orders

[Horn, Hazel V via Histonet]

In the 25+ years I have worked at ACH we have never allowed a resident 
physician to order a pathology test.  I am now being asked why we do this?   I 
have no idea if there is a rule from Medicaid or insurance providers that all 
orders must be from a staff physician.   Does your hospital allow residents to 
place pathology orders?



Hazel Horn

Supervisor of Histology/Autopsy/Transcription

Anatomic Pathology

Arkansas Children's Hospital

1 Children’s Way | Slot 820| Little Rock, AR 72202

501.364.4240 direct | 501.364.1241 fax

hor...@archildrens.org

archildrens.org

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[Histonet] cpt codes

[Horn, Hazel V via Histonet]

Histonetters,
The cpt code for a colostomy stoma is 88304.  Would you consider and ileostomy 
the same cpt code?
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org







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[Histonet] FW: billing consults

[Horn, Hazel V via Histonet]

_Please respond privately if you would rather.  If you have billed for the 
professional component, do you bill the patient again for the consultation if 
you have sent it out 
to a pathologist for confirmation or diagnosis?
__

From: Horn, Hazel V via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, October 01, 2015 12:52 PM
To: histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] billing consults

Histonetters,


 1.  Please see the questions about billing for Pathology Consultations under 
varied circumstances as denoted below. For each circumstance, please specify 
whether you bill,
a) professional and technical only, or
b) professional, technical and consult.


 1.  If your response choice is b), please specify whether your payors 
typically pay for the consult charge.
==


 1.  The pathologist to whom the case was assigned has some doubts as to the 
best diagnosis after all attempts to classify the lesion.
 2.  The department has some disagreement about the diagnosis and sends the 
case to another pathologist to help decide the matter.
 3.  The clinician and/or patient requests a second opinion from another 
pathologist.



Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org<mailto:hor...@archildrens.org>
archildrens.org<http://www.archildrens.org/>







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[Histonet] billing consults

[Horn, Hazel V via Histonet]

Histonetters,


 1.  Please see the questions about billing for Pathology Consultations under 
varied circumstances as denoted below. For each circumstance, please specify 
whether you bill,
a) professional and technical only, or
b) professional, technical and consult.


 1.  If your response choice is b), please specify whether your payors 
typically pay for the consult charge.
==


 1.  The pathologist to whom the case was assigned has some doubts as to the 
best diagnosis after all attempts to classify the lesion.
 2.  The department has some disagreement about the diagnosis and sends the 
case to another pathologist to help decide the matter.
 3.  The clinician and/or patient requests a second opinion from another 
pathologist.



Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org







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[Histonet] FW: CAP All Common Checklist for Anatomic Pathology

[Horn, Hazel V via Histonet]

Dr. Raff,
If the links do not work, please let me know.  This is our procedure and our 
validation form.


http://ppm1.archildrens.org/dotNet/documents/?docid=13171=view

http://ppm1.archildrens.org/dotNet/documents/?docid=14275=view


Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: Lester Raff MD via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 24, 2015 10:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP All Common Checklist for Anatomic Pathology

Hello Histo-netters:

Do any of you have procedures written that cover the section on Test Method 
Validation/Verification? We do verifications/validations  of new antibodies but 
 don't really have a specific written policy/procedure. If any of you have a 
written procedure and would like to share, our lab would appreciate it!

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

https://downsizemaybe.wordpress.com
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[Histonet] dictation systems

[Horn, Hazel V via Histonet]

All,
We are looking for a new dictation system for grossing.  Will you please share 
what you use?
We still have a Lanier microcassette system and it's outdated and not made 
anymore.
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org







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Re: [Histonet] OJT Histotechs/Training

[Horn, Hazel V]

Joelle I agree with you.  But the problem is, no one knows we exist.  OJT is 
the only route for some/if not most positions to be filled.  We would all love 
to have a choice of educated ASCP registered techs to choose from.   I have an 
open position and no applicants.

Hazel Horn, HTL/HT (ASCP)
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: Joelle Weaver [mailto:joellewea...@hotmail.com] 
Sent: Thursday, May 14, 2015 2:35 PM
To: Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] OJT Histotechs/Training

We have discussed this on the histonet many times...
 
Most professions, and most if not all healthcare professions, require degrees 
and/or certification for entry. This is how the public, other medical 
professions ,and even HR-who do not know the technical- assess for our capacity 
to provide care and judge the skill level needed of the profession as they are 
looking in. We all know that this isn't always perhaps the best method to 
assess or measure some aspects of this profession, but this is what they work 
from. There are good and bad examples of both OJT and educated, formally 
trained histology professionals. However, education is more than learning 
facts, it helps develop many other facets of the person that are viewed as 
valuable to organizations. That is why it is used as a screening tool.  Please 
try to value the broader perspective.  Technical proficiency itself is probably 
not going to be enough as the future unfolds. Though it may seem unfair if you 
have worked for a very long time and learned a great deal through experienc
 e, the bottom line is that for some employers, some environments and outside 
groups- education, credentials and professionalism are the primary criteria 
they use to evaluate, and they pay and recognize accordingly. 
 
Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
 From: timothy.mor...@ucsf.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 14 May 2015 17:28:15 +
 Subject: Re: [Histonet] OJT Histotechs/Training
 
 Mike, yes, the vast majority of histotechs have been,  are, and will be OJT 
 (me included). The people who take on training these people have a 
 responsibility to do the best they can. Most techs end up learning whatever 
 their lab does and so have limited knowledge. I studied a full year for the 
 HT and passed fine, and later the HTL. In our small lab at the time we had a 
 broad array of testing in histology (specials, muscle histochem, immunochem, 
 electron microscopy), but I found out my true lack of knowledge when I went 
 to Saudi Arabia and worked with techs from other countries where they had 
 comprehensive bachelors-level programs required for ALL lab techs. Those from 
 the US, all certificated, where vastly under-educated compared to techs from 
 other countries. It was a bit embarrassing!
 
 Luckily we have online courses and degrees available now - not available in 
 the 1980's when I started. That is a tremendous advantage to those who are 
 willing to take advantage of it. Other than that it will be up to the lab 
 management to be sure the OJT tech gets the basic instruction according to 
 the requirements of the ASCP exam. That is the bare bones knowledge necessary 
 to function. Even then the experience in the lab is key to whether the 
 knowledge is just regurgitated or practiced. Lab management has a 
 responsibility to be sure good lab practices are ingrained during training. 
 It is a big job.
 
 As an aside, there are some people out there trying to break into histology  
 but do not work in a histo lab, or work in a lab that does not support their 
 desire to get certificated (which is practically criminal in my view). I 
 talked to a person recently who is working in a histo lab but is trying to 
 find a lab to do special stains they do not do in the lab they are working 
 in. Their lab will not buy them the reagents necessary and actually told this 
 person that they will not help them get certificated because they feel the 
 person will move on to get better pay elsewhere. 
 
 I agree with another thought expressed that finding a person excited about 
 getting into histology can lead to a good tech. I had a person just show up 
 cold one day saying he really wanted to work in the histo lab - he had 
 learned some histology in a research lab and did not realize it could be a 
 full time profession until he stumbled on our lab one day. He had a good 
 background but we had no histo jobs open, but we happened to have a new 
 grossing lab aid job opening and he managed to get that job. The expectation 
 is that he will eventually work his way into histology. He's happy to have 
 his foot in the door, and we are happy to have an enthusiastic person with a 
 plan for 

Re: [Histonet] Friday Trivia Question: Most specimen on a single case

[Horn, Hazel V]

227 blocks on a bone tumor.  Resident grossed it of couse!  It was a nightmare.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: Michael Mihalik [mailto:m...@pathview.com] 
Sent: Friday, May 08, 2015 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Friday Trivia Question: Most specimen on a single case

Please excuse the trivia query, but we've got a client who somewhat regularly 
creates cases with 100+ specimen.  I think the most I have ever seen is 127.

I'm curious how common this is.  What's the most specimen on a single case 
you've ever seen?

Thanks for your patience and experience.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369



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[Histonet] zero based position - Arkansas

[Horn, Hazel V]

Arkansas Children's Hospital in Little Rock will have a zero based position 
posted this week.This is a temporary position that could possibly work into 
a fulltime position if you are the right person for the job.  The zero based 
position will have no benefits.  The hours will be from 7:00am until 1:00pm, 
Monday through Friday.  Please contact me directly if you are interested.  
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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Re: [Histonet] IHC billing question

[Horn, Hazel V]

We have 2 billing codes for IHC.  One for the first IHC and another for all 
additional IHC's on the same case.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] 
Sent: Friday, May 01, 2015 8:13 AM
To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC billing question

I do it every day  - change every first to 88342.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, April 30, 2015 5:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC billing question

Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for 
IHC from our CoPath stain dictionary since you couldn't tell whether a 
Cytokeratin-7 was being performed as an 88341 or as an 88342.  Now, as you 
might have expected,  none of the inpatient IHC testing has been accounted 
for (the outpatient IHC has been billed manually from the pathology report), 
and they want someone to go back and enter all the CPT codes into the system 
(hopefully, not me!).  Has anyone else encountered this problem?  Thanks (I 
think).

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] IHC billing question

[Horn, Hazel V]

Meditech

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] 
Sent: Friday, May 01, 2015 10:51 AM
To: Horn, Hazel V; 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: RE: IHC billing question

And what LIS to you have? 

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
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intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email. 


-Original Message-
From: Horn, Hazel V [mailto:hor...@archildrens.org] 
Sent: Friday, May 01, 2015 11:50 AM
To: Weems, Joyce K.; 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: RE: IHC billing question

We have 2 billing codes for IHC.  One for the first IHC and another for all 
additional IHC's on the same case.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org]
Sent: Friday, May 01, 2015 8:13 AM
To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC billing question

I do it every day  - change every first to 88342.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, April 30, 2015 5:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC billing question

Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for 
IHC from our CoPath stain dictionary since you couldn't tell whether a 
Cytokeratin-7 was being performed as an 88341 or as an 88342.  Now, as you 
might have expected,  none of the inpatient IHC testing has been accounted 
for (the outpatient IHC has been billed manually from the pathology report), 
and they want someone to go back and enter all the CPT codes into the system 
(hopefully, not me!).  Has anyone else encountered this problem?  Thanks (I 
think).

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
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are not the intended recipient, or an employee or agent responsible for 
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[Histonet] RE: Electronic monitoring systems

[Horn, Hazel V]

Our hospital's building automation takes care of this for us.  If the tissue 
processor, freezer, etc. goes into alarm, they call me.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Ann 
Jones
Sent: Tuesday, January 20, 2015 1:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Electronic monitoring systems

Hi all,
I was wondering - a little while ago there was mention on the blog of equipment 
(processors) being monitored and alerting the 'manager' if they went down. I'm 
sorry I cannot remember what system this was. What do you all use?
Also, what temperature control monitor is everyone using? Ours has flaws and we 
are gathering information.
Can the two systems be combined under one company?
Any help is appreciated.

Thank you!
Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.commailto:mjo...@metropath.com
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[Histonet] RE: Recycling alcohol

[Horn, Hazel V]

With Ethyl alcohol you will need a license and will have to keep records.  With 
reagent grade alcohol none of that is necessary.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Monday, January 19, 2015 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycling alcohol

We are planning to recycle alcohol in the new lab I am working with.   
Previously I always used an alcohol blend such as the Flex products.  However 
at this new lab we are going to only process biopsies so I believe I can get by 
using ethanol and not a blend.   We will be getting our alcohol from 
Thermofisher.  Can anyone tell me which alcohols they are using for 
dehydration?  Reagent alcohol or ethyl alcohol.  Obviously we will make our own 
concentrations from a 100%.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


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[Histonet] RE: Rubber mats for pinning specimens

[Horn, Hazel V]

We use Styrofoam and break it to the right size.  Get them out of shipping 
boxes.  Free!

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice 
Frederick
Sent: Tuesday, November 18, 2014 10:25 AM
To: Bea DeBrosse-Serra; Dennis Hahn; 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Rubber mats for pinning specimens

We make up paraffin trays, either in a cafeteria tray (sssh) or in the lid 
of a slide box if it's for something small. Works like a charm and supplies are 
at hand. Besides that, paraffin floats in a formalin tank as well. You could 
have custom cut trays and still use your paraffin.
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea 
DeBrosse-Serra
Sent: Tuesday, November 18, 2014 10:21 AM
To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Rubber mats for pinning specimens

Have you tried to pin on large cork sheets?

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn
Sent: Tuesday, November 18, 2014 8:18 AM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Rubber mats for pinning specimens

Can anyone tell me if there is a company that sells the rubber mats for pinning 
open larger specimens, such as colons? We are currently using cooled paraffin 
as our pinning surface. I have found one company that sells small pans with the 
rubber already inside of it, but I'm looking for something we can cut to fit 
our current containers, no small specimen pans needed. The pathologists have 
also stated that they do NOT want cork.

Thanks again,
Dennis

Dennis Hahn, HT (ASCP)
Histology Lab Supervisor
Laboratory Safety Officer
Cook Children's Medical Center
801 7th Avenue
Ft. Worth, TX 76104
(682) 885-6133

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[Histonet] tissue tek 5 embedding center

[Horn, Hazel V]

Can anyone tell me if the Tissue Tek 5 has a work light?
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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RE: [Histonet] Flames at embedding centers

[Horn, Hazel V]

Yes, the good old days!And, we survived!

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children’s Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, November 18, 2014 3:14 PM
To: Blazek, Linda; Jay Lundgren; Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flames at embedding centers

Like!  ditto! Paula Pierce,BS,HTL(ASCP)HT President Excalibur Pathology, Inc. 
5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX 
www.excaliburpathology.com
  From: Blazek, Linda lbla...@digestivespecialists.com
 To: Jay Lundgren jaylundg...@gmail.com; Ludlow Patricia lud...@hhsc.ca 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
 Sent: Tuesday, November 18, 2014 3:03 PM
 Subject: RE: [Histonet] Flames at embedding centers
   
Grinning and remembering the good old days.  What's more fun is the look of 
horror on the faces of the young ones when they hear it!



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Tuesday, November 18, 2014 2:42 PM
To: Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flames at embedding centers

    Ahh, the good old days of walking into the lab and lighting all the Bunsen 
burners first thing in the morning.  I didn't have any hair on my knuckles for 
years.  Embedding with a Coke on the cold plate, and a smoke in the ashtray 
next to you, anyone?  Good times.

    Now we have to use forceps warmers and change forceps between specimens.  
If you get 3 or 4 pair of forceps, one will always be hot enough to use.  Also, 
there are embedding centers with heated forceps, which I love .
    Just remember to clean out the wells of the forceps warmers every day to 
prevent cross contamination.  Cotton applicator swabs work great for this.  And 
always keep a towel or gauze handy to wipe the tips of the forceps between 
specimens.  Forceps warmers unfortunately don't incinerate any stray tissue 
like a Bunsen burner did.

                      Sincerely,

                              Jay A. Lundgren, M.S., HTL (ASCP)




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[Histonet] RE: Cold plates for icing blocks?

[Horn, Hazel V]

We are a small lab and we each use a cold plate from the embedding station.  
Our microtomes sit on either side of the embedding station  and each side has a 
cold plate.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, November 03, 2014 2:06 PM
To: 'Morken, Timothy'; Histonet
Subject: [Histonet] RE: Cold plates for icing blocks?

I have an old cold plate from an old embedding center that one of my techs 
uses. I do wish someone would make a reasonably sized/priced one for individual 
use.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, November 03, 2014 2:29 PM
To: Histonet
Subject: [Histonet] Cold plates for icing blocks?

Does anyone use a cold plate, like that used for embedding, for icing blocks 
for sectioning? Just an idea

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
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please contact the sender by reply email and destroy all copies of the original 
message.



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RE: [Histonet] RE: New CPT Codes for IHC

[Horn, Hazel V]

I would like the correct answer for this.  I have always billed as Joyce 
states.  But a sister hospital says it's like Andrew's email.  Who is right?

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, October 06, 2014 3:41 PM
To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New CPT Codes for IHC

Sorry, I left that part out. We don't do them, so I just didn't think. 

I understand the 88343 codes to be for multiple stains on the same slide. The 
first is 88342 additional 88343 - e.g. PIN4.

So has that changed? 


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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regarding the error in a separate email. 

-Original Message-
From: Andrew Horvath [mailto:ahorv...@cogipath.com] 
Sent: Monday, October 06, 2014 3:36 PM
To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New CPT Codes for IHC

Actually, for non-Medicare if there are multiple stains performed on a block, 
where the initial CPT is 88342, any subsequent billing for stains on that block 
would actually be 88343 from the information I see.  

Please refer to the links below for more information...

http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf


https://www.aapc.com/memberarea/forums/showthread.php?t=101402



quote from the forum:

Quite and uproar about this in our office - and a lot of confusion. Go to page 
360 in this document to read Medicare's decision about this. (inactive link 
removed)


The CPT Editorial Panel revised the existing immunohistochemistry code, CPT 
code 88342 and created a new add-on code 88343 for CY 2014. Current coding 
requirements only allow CPT code 88342 to be billed once per specimen for each 
antibody, but the revised CPT codes and descriptors would allow the reporting 
of multiple units for each slide and each block per antibody
(88342 for the first antibody and 88343 for subsequent antibodies). We believe 
that this coding would encourage overutilization by allowing multiple blocks 
and slides to be billed. 
To avoid this incentive, we are creating G0461 (Immunohistochemistry or 
immunocytochemistry, per specimen; first single or multiplex antibody stain) 
and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each 
additional single or multiplex antibody stain (List separately in addition to 
code for primary procedure)) to ensure that the services are only reported once 
for each antibody per specimen. We believe this will result in appropriate 
values for these services without creating incentives for overutilization.

Some of our docs are participating in conference call today with CAP to help 
clarify what this all means.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Friday, October 03, 2014 12:33 PM
To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: New CPT Codes for IHC

For non-Medicare it is per block - CPT code 88342.

For Medicare - per specimen. G0461 for the first antibody and G0462 for each 
additional different ab.

Best, j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, 
Adesuyi (Banjo)
Sent: Friday, October 03, 2014 2:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New CPT Codes for IHC


  Hi,
I hope you guys are doing 

[Histonet] RE: Decalcified Bone Eosin Overstaining

[Horn, Hazel V]

When the bones are over decaled there will be very little hematoxylin staining 
and too much eosin.  You can use a buffer before the hematoxylin to aid the 
hematoxylin in staining.  I think one/two dip(s) in eosin will be sufficient.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Thursday, October 02, 2014 1:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decalcified Bone Eosin Overstaining

Hey histonetters! I'm currently doing some HE Staining for EDTA Decalcified 
Bone Tissue and it seems that the tissue is staining very intensely with 
Eosin...perhaps too much. Was curious to see from some of the more experienced 
histotechnicians out there which methods you guys have used in the past to 
perhaps fix an issue like this. Here is my protocol that I have used once I 
have sectioned the paraffin embedded tissue. I have omitted the 1 second Acid 
Alcohol Dip in a few of my trials and it seemed that this method worked great 
to keep the tissue from overstaining with Eosin. However, this is an important 
step for differentiation of the Hematoxylin...so I'm a little puzzled about 
what to do next. Perhaps wash with tap water again after the Acid Alcohol?


1. Xylene - 3 Minutes

2. Xylene 3- Minutes

3. Xylene - 3 Minutes

4. 100% EtOH - 3 Minutes

5. 100% EtOH - 3 Minutes

6. 95% EtOH - 3 Minutes

7. 70 % EtOH - 3 Minutes

8. Filtered Harris Hematoxylin (Commercial Purchase) - 6 Minutes

9. Wash with running Tap water

10. 1 second dip in 0.5% 12M HCL Acid Alcohol

11. Eosin Y Solution - 1 Minute

12. 70% EtOH - 2 Minutes

13. 70% EtOH - 2 Minutes

14. 95% EtOH - 2 Minutes

15. 95% EtOH - 2 Minutes

16. 100% EtOH - 2 Minutes

17. 100% EtOH - 2 Minutes

18. Xylene - 1 Minute

19. Xylene - 1 Minute

20. Xylene - 1 Minute


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
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[Histonet] RE: Surgical Pathology reports

[Horn, Hazel V]

Debby there is critical test results to call.  Unexpected findings such as 
malignant when benign were expected for example.   Our pathologists call and it 
is documented in the pathology report when they called and who they spoke to.   
If the clinicians are waiting on a diagnosis and results are delayed for 
special testing the pathologists will call and tell them what is going on with 
their case.  There is a CAP standard for this very issue, what you do about 
unexpected findings.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
Sent: Tuesday, September 23, 2014 12:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Surgical Pathology reports

Do pathologists ever call pathology reports to a physician?  If so, what types 
of diagnoses do they call?  There are no critical test results in Pathology 
that CAP deems necessary to call as in other areas of the laboratory..  How do 
others handle this?

Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






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[Histonet] job opening in little rock, ar

[Horn, Hazel V]

Arkansas Children's Hospital, located in Little Rock, AR has a job opening in 
our histology lab.  We are a small lab serving the children of Arkansas. We 
perform special stains by hand, Immunos on a Bond and routine histology.  The 
position is the late shift position from 8am until 4:30pm, Monday-Friday.  We 
rotate call for major holidays.  Arkansas Children's Hospital has competitive 
salaries and good benefits.
Please apply online.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] RE: slide filing cabinets

[Horn, Hazel V]

We have no problems stacking the block cabinets from Tissue-Tek.  5 high will 
not be a problem.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kolman, 
Kimberly D.
Sent: Monday, August 25, 2014 12:06 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] slide filing cabinets

Dear Histo-netters:

 

I'm looking to re-arrange my paraffin block storage; currently the plastic 
block cabinets are on a shelving unit.  

 

Does anyone have any experience with how well the old Tissue Tek plastic 
6-drawer block cabinets stack - on top of each other?  Can I safely do a stack 
of 5?  And will they hold up well?

 

Thanks in advance.

Kim

 

 

Kim Kolman

 

Kimberly D. Kolman, HT, (ASCP)

VA Eastern Kansas Health Care System

Dwight D. Eisenhower VA Medical Center

Histology - 115

4101 S. 4th St. Trfwy.

Leavenworth, KS 66048

913-682-2000x52537

Fax: 913-758-4193

 

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[Histonet] BRAF by immunohistochemistry

[Horn, Hazel V]

Does anyone know of a place we can send a case to for BRAF immunohistochemistry?

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] RE: Bubble problems!

[Horn, Hazel V]

Melissa we are having the same problem.  We use a different mounting medium.  I 
will try Barbara's advice and try adding a bit of xylene to the media.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Thursday, August 07, 2014 8:12 AM
To: Melissa Ng; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Bubble problems!

Try adding xylene to the mounting medium to thin it.  About 10 ml of xylene to 
90 ml of mounting medium should do it.
Then, leave the bottle of mounting medium for about a day or two to allow any 
air that may have gotten mixed into to dissipate.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Melissa Ng 
melissa...@spdscientific.com
Sent: Thursday, August 07, 2014 7:59 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bubble problems!

Dear Histonet,



I am having a major problem with bubbles in my mounting media. I am currently 
using the Leica coverslipper and Cellpath mounting media (Phalate free). 
Micro-bubbles are observed on the slides after sometime of coverslipping. In 
fact, when I checked the media, I found lots of bubbles inside the bottle too! 
There were no bubbles in the bottle prior to placing the mounting media with 
the machine (I checked!). Also, we do not see any bubbles in the tubes that 
lead to the probe!



Previously, we were using the Leica mounting media (yellowish liquid) and there 
were no bubble issues. I have checked with other labs using the Cellpath 
mounting media (they use Leica coverslipper too) and no one seems to be having 
the same problem as us!



The Cellpath mounting media is way more viscous compared to that of the Leica 
media.



Has anyone here experienced a similar issue, and how have you resolved it?



Appreciate all the help I can get!



Best regards,

Melissa
Application Specialist
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[Histonet] RE: questions

[Horn, Hazel V]

Our cases are cut by priority.  And we don't process breasts. (children's 
hospital)

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Thursday, August 07, 2014 11:54 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] questions

I have a couple of questions to ask where there is no right or wrong answer, 
just curious as to the process that other labs use.
1.  After processing, how do you determine the order in which to cut and stain 
the blocks..numerical or priority driven?  2. Do you adhere to the 6-72 hours 
of fixation for breasts or make certain all breast tissue is fixed for a 
minimum time of, say, 24 hours but, of course no longer than 72?

I appreciate your responses and thanks for your time!!  I am retiring at the 
end of this year and trying to optimize some processes beforehand:).

Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962



  
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RE: [Histonet] On the lighter side...

[Horn, Hazel V]

41 years.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, August 07, 2014 2:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

36 years.

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Sullivan, Beatrice bsulli...@virtua.org
To: 'Douglas Porter' doug.por...@caplab.org; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Thursday, August 7, 2014 1:51 PM
Subject: RE: [Histonet] On the lighter side...
 

44 years here.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???



Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street                                    
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

mailto:doug.por...@caplab.org doug.por...@caplab.org 

http://www.caplab.org/ www.caplab.org                              



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RE: [Histonet] Safety shower

[Horn, Hazel V]

Our Maintenance department tests our showers each month.  We test our eyewash 
stations each week.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alicia Lange
Sent: Friday, July 11, 2014 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safety shower

I test our shower and eye wash weekly. We had a consulting company prepare us 
for an OSHA inspection and we were told then the testing needed to be done 
weekly for both. The temperate, flow, and clarity aren't necessarily tested 
just function. I use a 35 gallon trash bin to collect the water from the 
shower. I only run the shower enough to flush about 5 gallons out.
Hope this helps!

Alicia Lange HTL (ASCP)

 On Jul 11, 2014, at 10:00 AM, histonet-requ...@lists.utsouthwestern.edu wrote:

 Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

 To subscribe or unsubscribe via the World Wide Web, visit
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 When replying, please edit your Subject line so it is more specific
 than Re: Contents of Histonet digest...


 Today's Topics:

   1. RE: safety shower (Amber McKenzie)
   2. Job Opportunity (Baldwin, Kathy)
   3. RE: Cytokeratin AE1/AE3 (Cartun, Richard)
   4. formalin/xylene monitoring (Ronda Mire)
   5. RE: formalin/xylene monitoring (Vincent Rivera)
   6. Re: formalin/xylene monitoring (Ronda Mire)


 --

 Message: 1
 Date: Fri, 11 Jul 2014 14:36:14 +
 From: Amber McKenzie amber.mcken...@gastrodocs.net
 Subject: [Histonet] RE: safety shower
 To: John Shelley jshel...@sanfordburnham.org, Nancy Schmitt
nancy_schm...@pa-ucl.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Message-ID: 5a33c952bb67f4468af1f36d739212bc011250e...@jerry.gia.com
 Content-Type: text/plain; charset=iso-8859-1

 What should the water volume be? Temperature and clarity?  How do you test 
 these things?

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John
 Shelley
 Sent: Wednesday, July 09, 2014 12:39 PM
 To: Nancy Schmitt; histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RE: safety shower

 Our safety group here had built a neat little contraption using flexible PVC 
 piping and making it circular, slightly larger than the shower head and 
 placed a shower curtain and rings on it. When they pull the lever and use 
 their system the water falls into a garbage can for collection. They come 
 around once a month to check it out.

 Kind Regards!

 John J Shelley
 Research Specialist, Histology Core Facility

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy
 Schmitt
 Sent: Wednesday, July 09, 2014 1:29 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] safety shower

 How often are you testing your safety shower ?  Are you always checking for 
 water volume,  temperature and clarity?

 I appreciate your thoughts.,

 Nancy Schmitt MLT, HT(ASCP)
 Dubuque, IA



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 --

 Message: 2
 Date: Fri, 11 Jul 2014 14:49:35 +
 From: Baldwin, Kathy sbald...@mhhcc.org
 Subject: [Histonet] Job Opportunity
 To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
 Message-ID: 9e3d7cb10c43496c8e91f4e30af8a...@exch02.mhhcc.org
 Content-Type: text/plain; charset=us-ascii

 Histology Tech position: Full time Monday thru Friday.  Jasper, Indiana 
 47546Jasper is a regional center in southwestern Indiana, noted for its 
 German Catholic ancestral roots Jasper has often been called the Wood 
 Capital of the World, boasting a large number of furniture companies, 
 including 

[Histonet] dissecting microscope

[Horn, Hazel V]

We are looking for a dissecting microscope.  Nothing real fancy and it does not 
have to be new.
Thanks.  Please email me and I will call you.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] RE: GI biopsies and special stains

[Horn, Hazel V]

We do the same as Andrea.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bilger, Andrea
Sent: Monday, June 09, 2014 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI biopsies and special stains

Tests need to be medically necessary, therefore, we do not auto order stains on 
all GI biopsies.  We wait until the HE is screened and then order H Pylori by 
IHC on only those biopsies that need it.

Andrea Bilger, HTL
Team Leader, Histology
York Hospital
1001 S. George St.
York, Pa.  17405
(717) 851-5040


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RE: [Histonet] Basement Lab

[Horn, Hazel V]

We too have windows!!

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Monday, May 05, 2014 10:15 AM
To: Shirley A. Powell; susan.wal...@hcahealthcare.com; suetp...@comcast.net; 
jrsmallw...@bell.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Basement Lab

We have a beautiful window viewI would insist on windows!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Monday, May 05, 2014 11:13 AM
To: susan.wal...@hcahealthcare.com; suetp...@comcast.net; jrsmallw...@bell.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Basement Lab

Because we are explosive and flammable?  I know it is terrible, 
they need us but want to hide us.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Monday, May 05, 2014 3:19 AM
To: suetp...@comcast.net; jrsmallw...@bell.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Basement Lab

Why do they always want to put us in the basement? We have a lot of the 
hospitals explosives and flammables??
Still, as long as they have a good ventilation (you often need a roof or 
outside wall to do this) it might be ok. I hope they have good environmental 
engineers.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Saturday, May 03, 2014 3:51 PM
To: John Smallwood
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Basement Lab

Not a good idea

Sent from my iPhone

 On May 3, 2014, at 12:10 PM, John Smallwood jrsmallw...@bell.net wrote:
 
   Our small Hospital with growth plans, is considering a new Laboratory in 
 the basement of the planned tower. I consider this a less than desirable 
 location. Spills , fumes, chemical allotments etc. What are Histonet members 
 thoughts and ideas ??
 
 Than you,
 John Smallwood, MLT.
 London, Ont. Can.
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[Histonet] oil red o on cytology

[Horn, Hazel V]

What kind of control do you use when looking for fat in a BAL specimen?  This 
stain is done on a cytospin slide.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] air exchanges

[Horn, Hazel V]

Are there a set number of air exchanges for a histology lab?  References?
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] RE: TAT for Transplant Biopsies

[Horn, Hazel V]

We accept biopsies until 10:30 a.m.  Monday through Friday.   If it is an 
extreme emergency, we will accept a later cut off time and someone stays to 
take care of it.   Yes, we will process transplant biopsies on weekends and 
holidays.  We do not process the biopsies at night.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, January 09, 2014 6:47 PM
To: Histonet
Subject: [Histonet] TAT for Transplant Biopsies

For those of you seeing biopsies of transplant kidney, liver, and heart, what 
is your cut-off time for accepting a specimen for same-day processing?  Do any 
of you handle specimens at night, weekends, or holidays?  Thank you!



Richard



Richard W. Cartun, MS, PhD

Director, Histology  Immunopathology

Director, Biospecimen Collection Programs

Assistant Director, Anatomic Pathology

Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 972-1596 Office

(860) 545-2204 Fax

richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org

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RE: [Histonet] tea bags

[Horn, Hazel V]

We use the same as Mike.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Taylor
Sent: Friday, December 20, 2013 4:04 PM
To: Mike Pence; histonet-boun...@lists.utsouthwestern.edu; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] tea bags

Hi Mike.

We have used teabags that we have obtained from a company in the United States.

They are 2.5 x 2.5, sealed on three sides and are perfect for filtering 
cellular material in preparation for processing.

The Company is called Sunburst Bottle, 4200 Commerce Court, Suite 206, Lisle, 
IL. 60532. Tel: (877)9254500

Bags 2.5 x 2.5 Bleached Paper. 500 $0.06ea. 5000 $0.05ea. They do provide 
samples for evaluation.

Hope this is helpful.

Alan

Alan Taylor BSc(Hons). FRMS.
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ. UK
www.microtechnicalservices.co.uk

- Original Message -
From: Mike Pence mpe...@grhs.net
To: histonet-boun...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Sent: Friday, December 20, 2013 3:03 PM
Subject: [Histonet] tea bags


Hey all,
Where is everyone getting their tea bags for filtering endo specimens?  I 
cannot find the company that we were using for our bags. They are paper and 
measure 2 ½ x 2 ½ and are sealed on three sides. They come in a strip of bags 
all attached to each other on one side and about 100 to a ziplock bag.

Thanks in advance for the info,
Mike Pence
GRHS
AP Supervisor
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RE: [Histonet] RE: Yahoo link

[Horn, Hazel V]

I remember participating in the health study in the 80's.   Xylene and 
Formaldehyde levels are monitored in all labs.  If our hospitals/research 
centers would allow us to forward that information on to someone who could 
compile data it would be a starting place for a health study.

I strongly believe this profession can be danger to health.  I can name at 
least 10 histotechs who have died from cancer.  Yes, it may have been when they 
were older but I feel certain it was from all the chemical exposure in 
histology before safety became a priority.   I know others will remember no 
ventilation, smoking and eating in the lab, no real requirements for gloves, 
etc. There were also no MSDS information available and we were exposed 
through lack of knowledge.

I believe the lab is much safer today with all of the safety precautions we 
take and the knowledge we have on chemicals/stains and their toxicity.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Wednesday, December 04, 2013 8:24 AM
To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Yahoo link

Couple of studies that I know of.

One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH 
Symposiums, and did different tests on people who volunteered to participate. 
Published findings in the late 1980's that said that histotechs had lower 
pulmonary function than average population, and decreased memory, equilibrium 
and dexterity than the general population.

In Letters to the Editor, people pointed out statistical flaws (low numbers of 
participants, for example). I also feel there were flaws, such as testing 
people after traveling over time zones, who were up late at the parties, and 
had possibly been drinking the night before. There was no way to measure how 
much exposure to formaldehyde or xylene people were really exposed to. I didn't 
participate, but if I though the amount I was being exposed to was medium, 
someone else being exposed to the same amount might have said low amount and 
someone else could have said high amount. And the studies would say therefore 
the low pulmonary exposure was due to histotechs being exposed to formaldehyde. 
But who could say it was due to that chemical, and not due another chemical, or 
due to the fact that at the same time, people were smoking in the lab I was 
working in, which was a small space.

Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy 
outcomes following gestational exposure to organic solvents. They interview 
women who were pregnant and working with organic solvent, so painters for 
example, so not histotechs only. They compared them to women of same age, same 
number of children, similar profession not exposed to organic solvents. 
They found that if the exposed women were having symptoms (breathing problems, 
rashes), they had a higher percentage of miscarriages and babies with 
deformities. If there were no symptoms, they had the same, and even lower, 
percentages than comparable women giving birth who were not exposed.

What histology needs is something like the nursing organization in the US has 
been doing for over 40 years. Everyone who is a member of the nursing society 
is sent a survey (I think every year), and asked to have physicals information 
released from their doctors to the organization (It's voluntary to 
participate). But they have 40+ years of data from hundred of thousands of 
women, of all ages. They can mine a wealth of medical data from this.

NSH (and ASCP) may not have enough histotechs in their organization to pay for 
the type of survey needed, to continue on for decades. We would need 
supervisors and bench techs to participate for decades. And probably have to 
mail them several formaldehyde and xylene monitors every year, or every couple 
of years, to collect real numbers of how much people were exposed to. 
And the surveys were pages and pages long (my mother was a nurse who 
participated from the beginning, so I've seen them), and asked lots of 
questions about health and diet and smoking and personal issues, in addition to 
questions about what types of chemical we work with an how much and how often. 
And how do we measure ventilation in all these places of work?

I hope someone comes up with some studies that can help us figure out if 
histotechs are being exposed to enough chemicals that could be causing these 
different diseases in humans. There are animal studies, but not human.

And remember, people in general, including histotechs, are living longer. If we 
live longer, we are more likely to have chronic diseases like 

RE: [Histonet] Specimens from surigical units

[Horn, Hazel V]

We have a similar system.  When pathology specimens are taken in surgery a 
notification is generated and sent to our printer.  We reconcile the 
notifications with the requisitions when the specimens are received.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Wednesday, November 20, 2013 10:09 AM
To: Vickroy, Jim; histonet
Subject: RE: [Histonet] Specimens from surigical units

We use the hospital LIS. All specimens/cases are entered and ordered by Surgery 
and they are then received by Surgical Pathology. You are able to effectively 
track w/ pending lists and a full manifest/reconciliation process.

William DeSalvo, BS HTL(ASCP)

 From: vickroy@mhsil.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 20 Nov 2013 10:03:45 -0600
 Subject: [Histonet] Specimens from surigical units
 
 
 We are a large pathology department and process over 35,000 surgical 
 accessions each year.  I am interested in learning how other pathology 
 departments keep track of the specimens that are removed in surgery to make 
 sure they are received in pathology.  Obviously all specimens received by 
 pathology are entered into the pathology accessioning system however the 
 question has come up to how do we know that every specimen taken in surgery 
 for pathology is actually received in pathology.  A couple of our surgical 
 units utilize volunteer couriers since the surgical units are not near 
 pathology.   A couple of other units like special procedures and cardiac 
 surgery have a simple notebook that is a signoff when the specimens are taken 
 to pathology, however from there is no tracking system from the main OR or 
 outpatient surgery. In otherwords a specimen could be removed in the OR that 
 was supposed to be sent to pathology does not arrive in pathology.  Obviously 
 this doesn't happen often but we are trying to close the potential for an 
 occurrence.   Specimens removed in surgery are logged into the surgical OR 
 report however  there is no current way for us to track that everything 
 removed that day that was supposed to go to pathology actually made it to 
 pathology.   Any ideas?
 
 Jim Vickroy
 
 James Vickroy BS, HT(ASCP)
 
 Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical 
 Center
 217-788-4046
 
 
 
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RE: [Histonet] On call hours required of staff

[Horn, Hazel V]

I would not ask my techs to take call for no pay.We have to respond to call 
in 1 hour.  The tech would not be able to make personal plans if they are on 
call.   

We are on call on long weekends.  We take turns taking call and are paid call 
pay.  If we get called in, it is overtime.   On the rare occasion someone needs 
to work Saturday, one of us volunteer and are paid call pay and overtime.

As a supervisor I am potentially on call all of the time.   If our tissue 
processor alarms, I am called.  I come in and I get call pay and overtime. 
(This is a rare occurrence)   If I am unavailable, my senior tech answers 
calls.  I also answer after hours calls from the lab.  Usually a phone call 
takes care of it.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, November 06, 2013 2:46 PM
To: Mike Pence; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On call hours required of staff

No pay has been mentioned. The call hours are to be covered initially by one 
person .
 
The tasks are potentially take care of anything occurring during those hours 
including stat specimens. The primary concern of management is that a 
refrigerator or the room temperature or humidity will go out of range when 
staff are not on site.  There is a 24 hour monitor for environmental stuff that 
will call to the person's cell phone, and anything else that occurs when no one 
is scheduled in the area will result in a cell call. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: mpe...@grhs.net
 To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] On call hours required of staff
 Date: Wed, 6 Nov 2013 20:34:17 +
 
 Will the techs be paid call pay? What is being asked of the techs to do on 
 call?
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
 Sent: Wednesday, November 06, 2013 2:26 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] On call hours required of staff
 
  Sorry if this re-posted- it got bounced back to me once.
  
 Please advise of the number of hours that your histology staff are scheduled 
 to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? 
 If you have a maximum # of on call hours by your lab or HR policies, that 
 would be most helpful.
 The proposal is to begin a usual 40 worked hours scheduled in the lab and 
 then ~ 15 hours overnight on call for each weekday,( M-F),  and potentially 8 
 hours or more each weekend day. I think this proposal is unreasonable, and 75 
 hours + 16 hours potential on call/week ( 91 hours on call?). 
 I would appreciate your thoughts , comments? I hope to derail this with 
 something more reasonable
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
 
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[Histonet] freezing spray

[Horn, Hazel V]

Our hospital has not allowed freezing spray to be used in the frozen section 
lab for many years.  We now have a new group of doctors who want to use the 
spray.  The docs think the frozen sections take too long to freeze.  Yet, they 
meet the frozen section TAT for more than 98% of our cases.  I think it's worth 
not using it for the one case where no one suspects TB but the patient will be 
positive.

Do you allow freezing spray in your frozen section lab?


Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] RE: Dress codes

[Horn, Hazel V]

We have a hospital dress code.  It is not just for  the  lab.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Tuesday, October 08, 2013 12:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Dress codes

Not sure if my mail is going through or not...

Thank you

Nancy Schmitt


Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
205 Bluff Street
Dubuque, IA  52001
563-556-2010 ext. 142
nancy_schm...@pa-ucl.com



From: Nancy Schmitt
Sent: Tuesday, October 08, 2013 8:58 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Dress codes

Good Morning-
I am looking for input on dress codes - specifically shoe and pants guidelines 
and what regulatory agency your information comes from.
It seems this can be a hot button topic:( Thank you for your input- Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
nancy_schm...@pa-ucl.commailto:nancy_schm...@pa-ucl.com




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[Histonet] RE: Equipment selection

[Horn, Hazel V]

We have the Leica stainer and coverslipper and we like it.  The only drawback 
to our stainer is it only has 1 oven.  But the stainer and the coverslipper 
have been reliable and are easy to operate.We have the Leica Bond for 
immunos and we love it!  It's user friendly, very reliable and we have many 
options for antibody vendors.   Leica is a great company for support.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Huggins, Haley 
- MRMC
Sent: Wednesday, September 11, 2013 3:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Equipment selection

Hi all,

I am trying to decide on what equipment to purchase. I have picked the 
processor, microtomes, embedding centers and a few other pieces, but now I 
still need to decide on the HE Stainer and the immunostainer. My options I 
have in front of me are for the HE stainer are Sakura Prisma stainer/film 
coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the 
Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any 
advice on any of these instruments would be greatly appreciated. I am trying to 
finalize my decision pretty quickly. Thank you in advance for any input.



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RE: [Histonet] Re: Unregistered HT

[Horn, Hazel V]

I agree!  You are wise.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Thursday, September 12, 2013 8:22 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Unregistered HT

Since somebody mentioned the Samurai Pathologist (who is now 74 years old and 
in his 50th year in pathology) -

I agree with most of what's been said here and I won't repeat it.

From the pathologist's viewpoint - remember that most pathologists are 
now
on salary (or soon will be) and don't have a dog in the fight about doing the 
job as cheaply as possible.

I think that a very large part of the problem is that most pathologists haven't 
a clue as to what goes on in the histology lab (that's why we cram cassettes 
full of fatty breast tissue), and that pathologists need to acquire this 
knowledge in residency, to the degree that they can teach and trouble-shoot or 
work with senior technologists who can. It's particularly important that 
pathologists learn to embed.

Edwards Deming was an economist who grew up in operations research during 
World War 2. After the War he tried to get the automotive industry to adopt his 
methods. The executives laughed at him (and still do in the business schools, I 
think), so he took his ideas to Japan, where they built the Japanese automative 
industry. Deming's major idea (if I understand him
correctly) was that workers need constant specific feedback about what they're 
doing.

I think that the establishment of effective feedback from pathologist to 
histotechnologist is the first step in solving the problem we've been talking 
about. And I think that means a pathologist sitting down with a 
histotechnologist and reviewing some of the day's slides every blessed day.

Bob Richmond
Samurai Pathologist
Maryville TN
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RE: [Histonet] Unregistered HT

[Horn, Hazel V]

Histology does not get the respect or the recognition because histologists do 
not report results.  All of the complex testing we do is overlooked because the 
pathologists report the results.   CLIA standards are based on result 
reporting.   The CAP has looked the other way for years because pathologists 
would hire unregistered techs.  If pathologists would demand only registered 
techs half our battle would be won. 

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Wednesday, September 11, 2013 8:08 AM
To: 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

And the reason so many have been fighting for this for years. If a lab were 
looking for a Medical Technologist there would be no question.


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Tuesday, September 10, 2013 7:32 PM
To: Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

As long as we do not need certification, licensure and minium education 
requirements we will not be recognized as Laboratory Professionals.



From:   Marcum, Pamela A pamar...@uams.edu
To: 'joelle weaver' joellewea...@hotmail.com, 'Emily Sours'
talulahg...@gmail.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date:   09/10/2013 01:12 PM
Subject:RE: [Histonet] Unregistered HT
Sent by:histonet-boun...@lists.utsouthwestern.edu



I agree we have huge gray areas and not all histology schools are as good as 
they could be for what we are facing in Histology.  I keep harping on the fact 
that until we are recognized as Laboratory Professionals we will stay in this 
limbo.  The rules determining complex testing should be revisited to what is 
done in Histology Laboratories today and not what we did 30 or more years ago.  
The Clinical Laboratory is now so automated it is hard to find anyone in most 
areas who can even remember doing any manual testing.  The Micro lab is the 
closest to being as manual as areas of Histology.

I am in a small market and finding a registered Histologist is harder for us.  
I would love to have 8 to choose from and interview.

Pam Marcum

From: joelle weaver [mailto:joellewea...@hotmail.com]
Sent: Tuesday, September 10, 2013 2:59 PM
To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

 Well I am  mostly clinical...but I think that organizations can set standards 
outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I 
had to submit all my transcripts from high school up through masters in 
addition to  proof of my ASCP certification, IHC qualification, continuing 
education, and professional association activity. There is a lot of gray area 
out there. They seem to have not had trouble getting applicants though ( and I 
know this varies by market), there were over 8 candidates for an HT opening, 
which I thought was a pretty good turn out.


Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: pamar...@uams.edu
 To: talulahg...@gmail.com; joellewea...@hotmail.com
 CC: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Unregistered HT
 Date: Tue, 10 Sep 2013 13:53:26 +

 Research is a different area and not controlled by CAP, CLIA and other
hospital licensing or accreditation organizations. We are bound by the rules of 
these organizations and while I agree with you to a point. We do need minimums 
for training and registration by recognized licensing bodies when patient 
tissue is being processed for histological examination. I am sure no one thinks 
of this often however; there are medical legal issues with insurance we have 
that do not apply for research. It is also clear that registration does not 
mean we don't have registered people who are not as good as they should be 

RE: [Histonet] Unregistered HT

[Horn, Hazel V]

Well said, Tim.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 11, 2013 10:54 AM
To: Histonet
Subject: RE: [Histonet] Unregistered HT

This discussion comes up every year and, of course is never resolved to our 
satisfaction because histotechs  really have only a little say in it.

Personally I think labs are better off with certified techs. Uncertified techs 
should start as lab assistants and earn their certification before getting a 
histotech title.  The histotech title should be for someone who at least starts 
as a general histotech who can do the gamut of histology work - embedding, 
cutting, HE, specials and IHC/ISH. From that base they can branch out or 
specialize. It would be a dream to require formal schooling, as with med techs, 
but there are so few schools that it is just impractical. A certification is a 
baseline that shows a person who is OJT has learned the minimum required to 
understand histotechnology.

However, because we don't report out any results on our own authority there is 
no impetus for a pathologist or institution to make the position more than it 
is: a technical position that does do require fancy work, but in the end only 
provides the materials for someone else to use for interpretation, decisions 
and reporting. We don't even pick the sample, the test or even determine how to 
do the test - that is all determined by the Technical Director of the lab, 
usually a board certified pathologist. Our primary job is to provide good 
quality materials so they can do their job well. That does involved a lot of 
knowledge, organization and skill, but it is not at the level of making actual 
patient care decisions.

Pathologists Assistants get a  bit more respect because they are required to 
make judgment decisions on how to sample a particular case. Even then, that 
sampling is strictly detailed in procedures developed by pathologists.

The most we could ask for is licensing that requires certain qualifications to 
be a histotech. However, that has some drawbacks as well, namely the 
restriction of the profession to licensed people, and so is a barrier to entry. 
Would it lead to pay raises? Does anyone have studies showing pay before and 
after licesure requirements?  It is questionable whether it enhances the 
quality of the lab since most histotechs are OJT anyway, and simple licensure 
may not increase actual quality of work by an individual. (does anyone know of 
any studies that look at quality in states with and without licensing?).

The CLIA requirements at least set a baseline for education, if not actual 
certification. Asking pathologists to support universal certification and/or 
licensure is problematic - many independent labs won't support that because, as 
in licensure, it decreases the pool and increases costs (ie, pay). Since the 
pathologist is the person deemed responsible for quality and lab results, 
setting the bar higher is only in the interests of the technologists, not the 
pathologist. Now, some enlightened pathologists understand that better-educated 
and better-trained techs are good for the overall.

So in the end the histotech community, along with a few enlightened 
pathologists have to lobby for anything they want. But what is that? Increased 
pay? More say in lab operations?

My experience is that you can rise as high as you want if you take 
opportunities that come up. But that may mean either spending many years in one 
place slowly moving up, or moving around to take other opportunities. It will 
depend on the individual. In either case using your time in the lab to learn 
whatever there is to learn, even in other departments, or on various 
committees, pays off in the long run. And that includes management. The last 
thing you should do is limit yourself to a job description someone gives you. 
Take that as the base line, not the limit.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, September 11, 2013 6:30 AM
To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

Histology does not get the respect or the recognition because histologists do 
not report results.  All of the complex testing we do is overlooked because the 
pathologists report the results.   CLIA standards are based on result

[Histonet] cap

[Horn, Hazel V]

CAP is in the house!

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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[Histonet] WT1 c terminus

[Horn, Hazel V]

Does anyone use this antibody and what vendor do you use?
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
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RE: [Histonet] Question - OR specimens to Pathology

[Horn, Hazel V]

Luckily our Frozen Section Lab is in the OR.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Thursday, August 01, 2013 4:52 PM
Cc: HISTONET
Subject: Re: [Histonet] Question - OR specimens to Pathology

OR - you can get a rubbermaid took box and slap a few biohazard stickers on it 
and put some formalin pads inside and you have a transport box for surgical 
specimens.
I happen to know that that is how this box was invented.




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] Hardware from surgery

[Horn, Hazel V]

Our legal department required us to write a policy and gave us a list of 
devices that must be tracked.  After they are photographed and serial numbers 
on the devices dictated, we return them to surgery to the Inventory Analyst.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Friday, August 02, 2013 11:49 AM
To: 'anita dudley'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Hardware from surgery

We hold ours for 2 years.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley
Sent: Friday, August 02, 2013 12:33 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hardware from surgery

What do others do with hardware from surgery?  Do you hold on to it for a 
longer period of time because of a possible legal case?   Thanks so much, 
everyone have a great weekend!!



Anita Dudley

Providence Hospital

Mobile, Al. 36695
  
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RE: [Histonet] Blade Rationing Follow-up

[Horn, Hazel V]

I agree with Pam.  The Thermo Premiers are excellent blades.  We prefer them.  
My personal opinion is the Accu-Edge are not as good as they used to be.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children’s Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Thursday, June 20, 2013 6:10 AM
To: Susan Walzer
Cc: histonet@lists.utsouthwestern.edu; tmoor...@gmail.com
Subject: Re: [Histonet] Blade Rationing Follow-up



I would disagree as we have used the Thermo Premiers and they are just as good 
and we are n ot paying for the name.  I h ave tried just about every new blade 
used in Histology going back to sharpening the big blades and if you look there 
are excellent blades out there. We changed from Accu Edge several years ago 
everyone was upset for a few weeks.  Then I offered them some back after a few 
Accu Edge packages were found  in my office.  Suddenly   the staff that could 
only use Accu Edge refused to use them as they had changed their minds. So you 
can find others if you are aware of quality and price.  There are some bad 
blades out there that we would not use however; there is no longer only one 
game in town for blades.   



Pam 

- Original Message -
From: Susan Walzer susan.wal...@hcahealthcare.com 
To: Thomas Jasper thomas.jas...@deaconess.com, tmoor...@gmail.com, 
histonet@lists.utsouthwestern.edu 
Sent: Thursday, June 20, 2013 2:16:16 AM 
Subject: RE: [Histonet] Blade Rationing Follow-up 

You can shop all the blades in the world( and we have tried many) but if you 
care about quality sections the Accu-edges are the only game in town. 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper 
Sent: Wednesday, June 19, 2013 3:20 PM 
To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] Blade Rationing Follow-up 

You could start with cost comparison of blades (since this whole thing started 
as a blade issue).  Reps are willing to let you demo some before committing and 
you might save money if everyone likes a less expensive blade.  You can do the 
same for paraffin and slides as well as reagent alcohols and xylene.  Again, 
these are things the manager should be getting after.  Does your lab adhere to 
Lean principles?  There may be some cost savings there if you can accomplish 
tasks more efficiently; get the work done in less time or cutting back on 
overtime.  It's a bit difficult to say as much depends on the nature and scope 
of your service.  All situations are unique - do you send a lot of work out?  
Could you take it on in-house?  Or are you trying to do things in-house that 
could be sent out and have a cost positive effect?  Lots of questions for the 
manager... 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore 
Sent: Tuesday, June 18, 2013 1:23 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Blade Rationing Follow-up 

I really appreciate everyone's constructive comments regarding my post on blade 
rationing.  Lots of you said there are many other ways to cut costs in the lab. 
 I would like to hear some of your suggestions so I can take them back to my 
manager.  I'd like to give her some legitimate alternatives to her proposal. 
Would like to contribute to solving the problem of cutting costs. 

Thanks again 



Teresa Moore, HT 
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[Histonet] RE: Retention and Disposal of medical hardware

[Horn, Hazel V]

We have a policy for hardware and if it includes any type of serial number, 
etc. it is sent back to surgery for the item to be documented as removed with 
the manufacturer.  We also photograph them and include documentation in the 
gross dicatation.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dieringer, 
Connie J.
Sent: Thursday, March 07, 2013 2:18 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retention and Disposal of medical hardware 

I was wondering what everyone out there is doing in regards to how long you are 
keeping medical hardware such as pins/screws/metal plates/pacemakers, etc. that 
come from surgeries and how you are disposing of them?  I know the CAP 
requirement for wet tissue is 2 weeks after the final report, but is hardware 
included in that?  Do they go through any sort of decontamination before 
disposing of them?  

Connie Dieringer, HTL (ASCP)
Histology Supervisor
Michael E. DeBakey VA Medical Center
Houston, TX
Rm. 3A-215A, Bldg. 100
Tel.:  713-794-7259
Pager:  281-551-0171
Email:  connie.dierin...@va.gov



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RE: [Histonet] Leica Coverslipper

[Horn, Hazel V]

We too have one that is a work horse.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Friday, March 22, 2013 9:35 AM
To: Contact HistoCare; kgrob...@rci.rutgers.edu; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Leica Coverslipper

I can honestly say we have used it for years and do not baby-sit it. 

Jeanine H. Bartlett
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact 
HistoCare
Sent: Friday, March 22, 2013 10:30 AM
To: kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Coverslipper

Hi, 

The alerts are not from problems or malfunctions, they're from normal 
operation.  For example the suction arm WILL alert if the cover glass is picked 
up and another is attached to the first one and somehow lands in a manner that 
makes the arm thinks the glass reservoir is empty. It WILL alert until someone 
quiets the alert, to let you know if the two racks are complete. It WILL alert 
once three racks have been coverslipped while it attempts to discharge a fourth 
rack and jam itself up.  

As someone mentioned previously, it definitely needs to be babysat just for 
normal operation. I agree that it is over-engineered , and yes the arm picking 
the slide up in the air is a poor design. The sakura uses an actuator to push 
the slide out and back in. In fact it's able to coverslip two slides in the 
same time the Leica does one. 

Although it is sufficient, It is not ideal for high volume operations. Personal 
preferences and contractual allegiances aside, the Sakura combo wins by a mile 
for bulletproof reliability and ability to handle large quantities without fail 
or alert.


--




I have the Leica stainer and coverslipper, and I don't have anywhere near as 
many problems with the coverslipper as  described by Contact below.

Mine alerts once in a while; if his alerts that much, then something is 
seriously wrong.  (The last time mine alerted that much, it needed a new 
brain-this is an older machine that had 5 circuit boards and one gave out-and 
one new sensor.  Still worth it to us to fix it.)  Anything as complex as 
staining and coverslipping robots will be fussy from time to time.  But I love 
my Leica!

Kathleen

Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
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[Histonet] RE: Leica CV5030

[Horn, Hazel V]

We love our Leica CV5030.  It rarely breaks a slide.  It works well with our 
Leica Bond labels.  I would buy another one.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski
Sent: Thursday, March 21, 2013 12:30 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Leica CV5030

This coverslipper is over engineered. No self respecting histologist would 
design something that holds the slide a foot in the air by two v shaped 
grippers. Gravity sucks and the slide falls. I have a box of DISTROYED slides 
that I show the repairman. Can you imagine Sorry Mr. Smith we can't read your 
biopsy because it splintered into pieces AFTER we did all the hard work. Good 
luck with that health of yours.
It is NOT compatible with Ventana labels, as the gum sticks to those grippers 
and the slide falls, or jams in the output rack.
To tell you the truth, I loved the old Leica coverslipper. It had a walking 
beam and never broke a slide.
I'm in the mood for a Sakaura.

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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[Histonet] RE: FNA Clia Guidelines

[Horn, Hazel V]

Our pathologists do it all.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, 
Jessica
Sent: Thursday, February 28, 2013 2:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FNA Clia Guidelines

Hi All,

Quick questionwhat are the Clia requirements for Fine needle 
aspirate procedures? Is it considered high complexity testing? And who prepares 
the slides when the needle is handed off?

Thank you,

Jessica Piche, HT(ASCP)



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RE: [Histonet] Waste Alcohol

[Horn, Hazel V]

The ability to put alcohol down the drain is regulated by your wastewater 
utility.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, February 25, 2013 3:53 PM
To: Scott, Allison D; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol

I always dumped the ethanol down the drain. I never recycled it because it 
takes almost 3 times more time that recycling xylene (3 times the cost) and the 
recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
René J.

From: Scott, Allison D allison.sc...@harrishealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol

Hello to all in histoland.  Is there anyone putting alcohol into a waste drum 
like for xylene.  We have a company that takes away our xylene.  Now the safety 
people are suggesting that we treat the alcohol the same way.  We have always 
poured the alcohol down the drain.  It seems very expensive.  Your help in this 
matter will be greatly appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287


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[Histonet] RE: Waste Alcohol

[Horn, Hazel V]

Our xylene and alcohol go into a waste drum to be disposed of.   We do recycle 
both but of course we still have waste to dispose of.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, February 25, 2013 2:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol

Hello to all in histoland.  Is there anyone putting alcohol into a waste drum 
like for xylene.  We have a company that takes away our xylene.  Now the safety 
people are suggesting that we treat the alcohol the same way.  We have always 
poured the alcohol down the drain.  It seems very expensive.  Your help in this 
matter will be greatly appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287


CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender 
by return e-mail and delete this e-mail and any attachments from your computer 
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To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability and 
Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or 
Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under Texas 
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If you are not the intended recipient, or any authorized representative of the 
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RE: [Histonet] Disposal of DAB-link to Princeton.

[Horn, Hazel V]

That's exactly what we do.  After the potassium permanganate /sulfuric acid we 
neutralize it and put in down the drain with copious amounts of water.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, 
Jr.
Sent: Thursday, December 06, 2012 10:45 AM
To: Jerry Ricks; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Disposal of DAB-link to Princeton.

Please correct me if I am wrong but if you use potassium permanganate sulfuric 
acid, do you not deactivate the mutagenic properties?  Then disposal would not 
require a hazardous waste container.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
NEW EMAIL: joewal...@rrmc.org
www.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet 
Recognition(r) and the Governor's Award for Performance Excellence


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks
Sent: Monday, December 03, 2012 2:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Disposal of DAB-link to Princeton.


DAB is a mutagen and potential carcinogen.

Waste needs to be disposed of as hazardous chemical waste.

http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm

Jerry





 From: cha...@yahoo.com
 Date: Wed, 28 Nov 2012 13:14:26 -0800
 To: cp...@x-celllab.com
 Subject: Re: [Histonet] chemical disposal
 CC: histonet@lists.utsouthwestern.edu

 Chemical hazardous waste.

 Sent from my iPhone

 On Nov 28, 2012, at 12:44 PM, Cynthia Pyse cp...@x-celllab.com wrote:

  Quick question for Histoland. I am having a debate about DAB 
  disposal. Our general manager ( non lab background) insists that the 
  liquid DAB can go into a biological hazardous waste. I disagree, it 
  is a chemical and needs to be disposed in the chemical hazardous 
  waste. What is everyone else doing to dispose of DAB. We are located 
  in NY, I do have those regs. Thanks in advance for any and all help.
 
  Cindy
 
 
 
  Cindy Pyse, CLT, HT (ASCP)
 
  Laboratory Manager
 
  X-Cell Laboratories
 
  20 Northpointe Parkway Suite 100
 
  Amherst, NY 14228
 
  716-250-9235 etx. 232
 
  e-mail cp...@x-celllab.com
 
 
 
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RE: [Histonet] Looking at cryostats

[Horn, Hazel V]

Leica!

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
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100 YEARS YOUNG!
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
kgrob...@rci.rutgers.edu
Sent: Tuesday, November 06, 2012 8:26 AM
To: histonet
Subject: [Histonet] Looking at cryostats

What's good out there these days?  I am currently looking at the Leica CM3050; 
are there other brands that I should consider?

Thanks so much,
Kathleen


Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

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RE: [Histonet] air drying special stain slides rather than dehydrate and clear

[Horn, Hazel V]

I suppose my age will show here but I was always taught to NEVER let slides dry 
out unless the procedure indicated such.   Is there no drying artifact when you 
let these slide dry before coverslipping?

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Tuesday, September 11, 2012 7:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] air drying special stain slides rather than dehydrate and 
clear

Hi,
 My choice to air dry rather than dehydrate in ETOH  xylene is based on 
the stain rather than the spooky xylene hazard boogyman. Yes, not using xylene 
if it is not really needed is not a bad idea, but the main reason I air dry 
some stains is the alcohols remove some of the stains. Ever have a beautiful 
Luxol Fast Blue bleach out on you? The most exasperating thing in the world!
Generally stains that end in water can easily be air dried. Something 
alcoholic like eosin or Movat's Pentachrome ending in alcoholic saffron might 
as well be finished traditionally. I air dry any stain that is counterstained 
in Nuclear Fast Red, Light Green, Methyl Green. I have air dried IHCs with no 
ill effects too. Don't try it with fluorescents though, that would be bad ... 
and pointless.
 I don't put them in an oven. I set them at the front of the fume hood and 
go do something else for a few minutes. If I want to rush it I close the sash 
to increase the flow rate for a bit. (Of course it is opened back up right 
after so the draft works properly.) Amos


On Tue, Sep 11, 2012 at 11:09 AM, histonet-requ...@lists.utsouthwestern.edu
 wrote:

 Message: 16
 Date: Tue, 11 Sep 2012 10:32:08 -0400
 From: Diana McCaig dmcc...@ckha.on.ca
 Subject: [Histonet] air drying special stain slides rather than
 dehydrate   and clear
 To: histonet@lists.utsouthwestern.edu
 Message-ID:
 dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca
 Content-Type: text/plain;   charset=us-ascii

 I was hoping to get information on why special stains are dehydrated, 
 cleared and mounted vs allowing them to be blotted dry, air dried then 
 coverslip.



 Every procedure I have ever encountered always indicates to dehydrate 
 and clear but I have heard where some labs are blotting the slides , 
 allowing to air dry (probably not set standard time) and dipped in 
 xylene prior to cover slipping.  Reason given is that the counterstain 
 gets washed out.  Wouldn't adjusting the times be a better resolution.



 I understand residual water could be present and cause long term 
 issues on storage but wanted some other opinions on this process.



 Diana

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RE: [Histonet] cassette printers/slide printers/slide labelers

[Horn, Hazel V]

I would love this same information. Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children’s Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd
Sent: Wednesday, August 29, 2012 8:30 AM
To: histonet
Subject: [Histonet] cassette printers/slide printers/slide labelers

  
I am looking for recommended brands, the pros/cons and cost advice on small 
scale cassette printers, slide printers or slide label systems for “on demand” 
printing at the gross stations and at  microtomes for tracking barcodes through 
the lab. NO software needed, these would have to interface into a new LIS 
system. 
 
Also, any advice/tips on yearly maintenance and consumable cost (cassettes, 
slides, labels, ink, print heads…..) would be appreciated!!! Vendors 
welcome to contact me by personal email.
 
 
Thanks,
 
Kelly 
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RE: [Histonet] Remote processor alarms

[Horn, Hazel V]

Our hospital monitors ours.   Our processor is connected to building automation 
and if it alarms off hours they call me.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of angela smith
Sent: Monday, August 27, 2012 3:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Remote processor alarms

What kind of remote alarms do you all have on your various processors when 
something goes wrong when you are not open but have processors running. So far 
I found sensaphone for the Peloris. I am interested in all types. 
Thank you
Angela Smith
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[Histonet] RE: Formalin and Operating Rooms

[Horn, Hazel V]

Debra and others:
All of our specimens are sent fresh because surgery says formalin cannot be in 
the operating rooms.  I'm told it comes from AORN, which is the association for 
operating room nurses.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debra Siena
Sent: Monday, August 20, 2012 5:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin and Operating Rooms

Hi All,

I would like to ask if anyone has heard of any new regulations or laws that 
state that the Operating Room can't have formalin available in the room so that 
they can place formalin onto the sample right away?  I was wondering if anyone 
has heard of this, if you could tell me more about where it is coming from so 
that I can access a copy of it.  We have had some inquiries and I have not 
heard of this.  I appreciate any help that you can give and sorry, that I don't 
have more information.  Best wishes.

  Debbie Siena, HT(ASCP)QIHC
StatLab Medical Products
Technical Support Manager
407 Interchange Street | McKinney, TX 75071
t: 800.442.3573 ext. 229 | f: 972.436.1369 dsi...@statlab.com | www.statlab.com


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[Histonet] RE: negative controls

[Horn, Hazel V]

For everyone performing immunos are you not running negative controls now with 
this new ruling from CAP?

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Romundstad, 
Pamela K
Sent: Friday, August 17, 2012 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20

Yes, the negative control updated change is in effect as of July 31. 2012. I 
contacted CAP personally this week and was directed to the revised ANP.22570. 
It only applies to polymer based detection systems (biotin-free).

Respectfully,
Pamela Romundstad HT, QIHC

Gundersen Lutheran

608-775-3139


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
histonet-requ...@lists.utsouthwestern.edu 
[histonet-requ...@lists.utsouthwestern.edu]
Sent: Thursday, August 16, 2012 12:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 105, Issue 20

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than Re: 
Contents of Histonet digest...


Today's Topics:

   1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline)
   2. Re: Two identifiers... (Bob Richmond)
   3. RE: Re: Two identifiers... (joelle weaver)
   4. RE: Re: Two identifiers... (Michael Mihalik)
   5. Negative Control (Pardue, Judith)
   6. RE: Special Stain documentation (hymclab)
   7. Peloris (Pardue, Judith)
   8. RE: RE: Two identifiers... (Pratt, Caroline)
   9. RE: RE: Two identifiers... (Tom McNemar)
  10. marker specific  for mouse fibroblasts (Kim Merriam)
  11. RE: RE: Two identifiers... (Pratt, Caroline)
  12. RE: marker specific  for mouse fibroblasts (Elizabeth Chlipala)


--

Message: 1
Date: Wed, 15 Aug 2012 18:07:46 -0400
From: Bass, Caroline ceb...@buffalo.edu
Subject: [Histonet] protocol for EGFP IHC in frozen rat brains
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: abc535ec-9fcd-45e0-957c-617fa7aa8...@buffalo.edu
Content-Type: text/plain; charset=Windows-1252

Hello Everyone,

I have some rat brains that should express GFP, the brains were collected fresh 
and snap frozen in dry ice/isopentane. They have been stored for several months 
in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an 
reserve these for mRNA collection. I would then like to take intervening 
sections to stain for GFP. I have a staining protocol for perfused, formalin 
fixed, sucrose protected floating brain sections that works fine. I'm a little 
more concerned about moving to unfixed sections. I know I need to cut 
everything on a cryostat and I assume that I have to process the thinner 
sections on the slide instead of free floating. What is the best way to 
proceed? Here are some specific questions?

1) what thickness should I cut, 50 um works great for my fixed sections.
2) should I fix these sections by either paraformaldehyde vapors or dunking 
in fix? If so, what kind/concentration?
3) could I then proceed as though this were a normal GFP immuno, with DAB stain?
4) should I used plus slides or subbed slides?
5) will the sections stay on without fixation?

Any and all suggestions would be appreciated!

Thanks,

Caroline


--

Message: 2
Date: Wed, 15 Aug 2012 21:11:12 -0400
From: Bob Richmond rsrichm...@gmail.com
Subject: [Histonet] Re: Two identifiers...
To: histonet@lists.utsouthwestern.edu
Message-ID:
caoksrh5bhasnonck7zg+04qjd23o0nfo+5t-8fjl71hs6af...@mail.gmail.com
Content-Type: text/plain; charset=ISO-8859-1

If a pathologist end-user may make an observation here:

When I pick up a slide and look to match it with the paperwork in front of me, 
it's a great help if the patient's name is on the slide, since names are always 
easier to read than numbers. Having the patient's name on the slide means I'm 
less likely to mix up two cases.
I can't do that with a bar code or a second essentially meaningless number.

This may however be a situation where good patient care must take a back seat 
to good management practice and good regulator 

RE: [Histonet] automated H+E and cover slipper

[Horn, Hazel V]

Same here for us...

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, 
Sandra C.
Sent: Wednesday, July 18, 2012 3:44 PM
To: Joe Hardin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] automated H+E and cover slipper

Leica Autostainer XL with CV5030 coverslipper and transfer station.  

This has been a real timesaver for us.  It automatically moves the slides from 
the stainer to the coverslipper.  It has been relatively trouble free.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin
Sent: Wednesday, July 18, 2012 3:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] automated H+E and cover slipper

Hi All,
I will be trying out new H+E autostainers and cover slippers soon. Does anyone 
have a favorite, and why? Thanks for your responses.


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RE: [Histonet] Leica CV5030 coverslipper issues

[Horn, Hazel V]

We have a CV5030 coverslipper and we have had a few issues but Leica has been 
quick to resolve them.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, May 21, 2012 1:15 PM
To: 'Linda'; histonet@lists.utsouthwestern.edu
Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; 
paul.raimo...@leica-microsystems.com
Subject: RE: [Histonet] Leica CV5030 coverslipper issues

We've had some issues that were mostly due to operator errors and lack of 
housekeeping, but they have been very good about correcting the problems.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda
Sent: Monday, May 21, 2012 1:58 PM
To: histonet@lists.utsouthwestern.edu
Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; 
paul.raimo...@leica-microsystems.com
Subject: [Histonet] Leica CV5030 coverslipper issues

Hello Everyone,


I purchase a brand new Leica CV5030 coverslipper, which I received at the 
beginning of February.  I have had non- stop issues with this coverslipper from 
it throwing slides, coverslips and now the sensor not working properly.

I have emailed Leica several times now requesting a new coverslipper.  If I 
have this many issues at three months what is it going to do in a year?

The tech service support person has been great with fixing all of the issues.

This is my reply I received from the President, North America, Jack Kenny-

We do not believe that it is appropriate at this point to replace this system. 
  We will continute to monitor the situation but not upgrade at this time.


I am not looking for an upgrade I would just like a new coverslipper that works.

Has anyone else had problems with Leica not replacing defective equipment?  
Please let me know.  How did you resolve the issue?

Thank you in advance,


Linda Dee, BGS, HT(ASCP)
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[Histonet] RE: CAP vs. CLIA

[Horn, Hazel V]

CAP does not consider us testing personnel. How they come by this is a mystery 
to me.   In a recent memo from CAP it describes this:

 Why does CAP require the completion of the Laboratory Personnel Evaluation 
Roster form and when was this process implemented? 

As part of CAP's deemed status with CMS as an accrediting organization, CMS 
required CAP to implement a more stringent process to document that accredited 
laboratories have appropriately qualified personnel and adequate documentation 
of personnel qualifications. The Laboratory Personnel Evaluation Roster form 
requires laboratories to confirm that personnel files contain the information 
necessary for laboratories to be in compliance with the CLIA personnel 
qualification regulations and CAP Checklist requirements prior to the 
inspection. It is also used by the inspection team to assist in the auditing of 
the records during the inspection to confirm compliance with the Checklist 
requirements. The process of completing the personnel form took effect in 
August 2009.

And goes on to say:
Do I need to list histologists on the Laboratory Personnel Evaluation Roster?  

Typical histologist duties (e.g., fixation, embedding, microtomy, staining and 
cover slipping) are not considered testing. Therefore, it is not necessary to 
list these personnel on the roster. However, if the histologist is performing 
any part of the macroscopic tissue examination which is considered high 
complexity testing, it is necessary to list those personnel on the roster. Such 
personnel must provide documentation at minimum of an associate's 
degree/transcripts or high school diploma or equivalent for individuals 
performing grossing at the same laboratory prior to September 1, 1997.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce
Sent: Wednesday, May 16, 2012 2:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP vs. CLIA


Can someone help me with the High Complexity Test with CAP vs. CLIA.
Thanks
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the new health

610 Oakmont Lane
Westmont, IL 60559

Office: + 630-203-6234
courtney.pie...@quintiles.com

clinical | commercial | consulting | capital


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error, please immediately notify the sender by reply e-mail and permanently 
delete this message and its attachments, along with any copies thereof. Thank 
you. 


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[Histonet] RE: CAP vs. CLIA

[Horn, Hazel V]

It was a CAP e alert dated April 2, 2012

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
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-Original Message-
From: Willis, Donna G. [mailto:donna.wil...@baylorhealth.edu] 
Sent: Thursday, May 17, 2012 8:42 AM
To: Horn, Hazel V; 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: CAP vs. CLIA

I have to say I disagree with this interpretation.  The commentary in the 
7/11/2011 checklists indicates that regulations apply to A laboratory must 
evaluate and document the competency of all testing personnel for each test 
system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and 
post-analytic steps used to produce a test result or set of results.  To me 
this includes both histology and pathology office staff.

This is the opinion on myself and our compliance person.  Hazel can you tell us 
where to find the CAP quote.

Thanks,

Donna Willis, HT/HTL (ASCP)
Histology Lab Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.wil...@baylorhealth.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, May 17, 2012 7:26 AM
To: 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: CAP vs. CLIA

CAP does not consider us testing personnel. How they come by this is a mystery 
to me.   In a recent memo from CAP it describes this:

 Why does CAP require the completion of the Laboratory Personnel Evaluation 
Roster form and when was this process implemented? 

As part of CAP's deemed status with CMS as an accrediting organization, CMS 
required CAP to implement a more stringent process to document that accredited 
laboratories have appropriately qualified personnel and adequate documentation 
of personnel qualifications. The Laboratory Personnel Evaluation Roster form 
requires laboratories to confirm that personnel files contain the information 
necessary for laboratories to be in compliance with the CLIA personnel 
qualification regulations and CAP Checklist requirements prior to the 
inspection. It is also used by the inspection team to assist in the auditing of 
the records during the inspection to confirm compliance with the Checklist 
requirements. The process of completing the personnel form took effect in 
August 2009.

And goes on to say:
Do I need to list histologists on the Laboratory Personnel Evaluation Roster?  

Typical histologist duties (e.g., fixation, embedding, microtomy, staining and 
cover slipping) are not considered testing. Therefore, it is not necessary to 
list these personnel on the roster. However, if the histologist is performing 
any part of the macroscopic tissue examination which is considered high 
complexity testing, it is necessary to list those personnel on the roster. Such 
personnel must provide documentation at minimum of an associate's 
degree/transcripts or high school diploma or equivalent for individuals 
performing grossing at the same laboratory prior to September 1, 1997.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax 
hor...@archildrens.org archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce
Sent: Wednesday, May 16, 2012 2:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP vs. CLIA


Can someone help me with the High Complexity Test with CAP vs. CLIA.
Thanks
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the new health

610 Oakmont Lane
Westmont, IL 60559

Office: + 630-203-6234
courtney.pie...@quintiles.com

clinical | commercial | consulting | capital


**  IMPORTANT--PLEASE READ   This 
electronic message, including its attachments, is COMPANY CONFIDENTIAL and may 
contain PROPRIETARY or LEGALLY PRIVILEGED information.  If you are not the 
intended recipient, you are hereby notified that any use, disclosure, copying, 
or distribution of this message or any of the information included in it is 
unauthorized and strictly prohibited.  If you have received this message in 
error, please immediately notify the sender by reply e-mail and permanently 
delete this message and its attachments, along with any copies thereof. Thank 
you

[Histonet] RE: CAP vs. CLIA

[Horn, Hazel V]

I certainly agree with you Jesus.  I felt like my face had been slapped.  

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org



-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com] 
Sent: Thursday, May 17, 2012 9:27 AM
To: 'Jesus Ellin'; Horn, Hazel V; 'Willis, Donna G.'; 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: CAP vs. CLIA

You hit the nail on the head!

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Thursday, May 17, 2012 10:24 AM
To: 'Horn, Hazel V'; 'Willis, Donna G.'; 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: CAP vs. CLIA

I am going to have to go there,, sorry all I know I am going to stir-up a 
hornets nest, but here it goes, don't we think that this is done in lue of the 
fact that CAP are representing the Pathologist interest and not the interest of 
the Technicians.  Times have changed and the CAP is asking for more and more 
from Anatomic Pathology questions every year, not only to include technical, 
but also instrumentation (simple and complex), as well as information systems, 
predictive markers, Digital Pathology ( a huge one), etc.  I think the CAP need 
to re-evaluate this and re consider what high complexity testing is, because 
CLIA defines it not the CAP.  Remember CAP enforces CLIA regulation as well as 
their own.  I would challenge this.  I feel the staff under me do more than 
turn a wheel, or place tissue in a mold.  With Passion comes a need to start to 
create change, we need this done.

Jesus Ellin  HT/PA ASCP, BSBE,MSBE
Yuma Regional Medical Center
Anatomic Pathology Supervisor

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, May 17, 2012 7:05 AM
To: 'Willis, Donna G.'; 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: CAP vs. CLIA

It was a CAP e alert dated April 2, 2012

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax 
hor...@archildrens.org archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org



-Original Message-
From: Willis, Donna G. [mailto:donna.wil...@baylorhealth.edu]
Sent: Thursday, May 17, 2012 8:42 AM
To: Horn, Hazel V; 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: CAP vs. CLIA

I have to say I disagree with this interpretation.  The commentary in the 
7/11/2011 checklists indicates that regulations apply to A laboratory must 
evaluate and document the competency of all testing personnel for each test 
system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and 
post-analytic steps used to produce a test result or set of results.  To me 
this includes both histology and pathology office staff.

This is the opinion on myself and our compliance person.  Hazel can you tell us 
where to find the CAP quote.

Thanks,

Donna Willis, HT/HTL (ASCP)
Histology Lab Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.wil...@baylorhealth.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, May 17, 2012 7:26 AM
To: 'Courtney Pierce'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: CAP vs. CLIA

CAP does not consider us testing personnel. How they come by this is a mystery 
to me.   In a recent memo from CAP it describes this:

 Why does CAP require the completion of the Laboratory Personnel Evaluation 
Roster form and when was this process implemented? 

As part of CAP's deemed status with CMS as an accrediting organization, CMS 
required CAP to implement a more stringent process to document that accredited 
laboratories have appropriately qualified personnel and adequate documentation 
of personnel qualifications. The Laboratory Personnel Evaluation Roster form 
requires laboratories to confirm that personnel files contain the information 
necessary for laboratories to be in compliance with the CLIA personnel 
qualification regulations and CAP Checklist requirements prior to the 
inspection. It is also used by the inspection team to assist in the auditing of 
the records during the inspection to confirm compliance with the Checklist 
requirements. The process of completing the personnel form took effect in 
August 2009.

And goes on to say:
Do I

RE: [Histonet] RE: Qualifications for grossing

[Horn, Hazel V]

Well said.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo
Sent: Wednesday, April 25, 2012 11:34 AM
To: Joanne Clark
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Qualifications for grossing

Joanna,

I wanted to take an opportunity to explain my, and most of my colleagues, 
feelings about CLIA '88 with respect to grossing standards. But I want to start 
by stating that this goes both ways, I also do not feel it is appropriate for 
an ASCP certified PA to be performing Immunohistochemistry, or other stains in 
the lab. Both histotechnicians (ologists) and PA's have a very clear role in 
the pathology laboratory. Both have very different training programs. Both HT's 
and PA's should be protected by law, and rules/regulations for each should be 
clear. One is not better than the other, and I certainly hope you do not think 
I have an opinion different from that. Both are highly qualified individuals in 
their area of expertise.

In many states, and I will use Florida as an example because that is what I am 
familiar with, there are clear definitions in the law as to whom can perform 
what tasks. In the State of Florida, a PA (regardless of training
level) is not to perform frozen sections. That State only allows Pathologists 
and HT's to cut a frozen. This is the result of much effort put in to changing 
those rules by the HT's in Florida. Clearly they saw PA's as a threat to their 
job, and took action. Not a problem, I am happy to let them do the frozen 
sections.

What was it about cutting a frozen section that the HT's thought a PA could not 
handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily 
trained in how to cut a frozen section, and it is generally considered our 
responsibility in most places in the US that I have seen, and I have seen many. 
Rarely, outside the State of Florida, do I see PA's that do not cut frozens.

Now, on to the issue of grossing techs. There are myriad reasons why I, and 
most of my peers, think it is not appropriate to utilize grossing techs.
For starters, and to be clear, the use of such techs serves one principal 
purpose to the pathologist's and institutions that employ them - to save money 
and increase their profits. They are not employed because they represent the 
clear choice for the utmost in patient care, and to suggest that is not just 
misleading, but completely false.

Grossing small specimens is never just about transferring tissue from a 
container to a block. Many tend to try and downplay the importance of that 
task, and overlook things that could be problematic without certain 
training/skills. And, there are many grossing techs that do larger cases, from 
gallbladders all the way up to mastectomies and beyond - all with no didactic 
education, no proficiency testing and no rotations through various types of 
insitutions.

I have never seen a study, but perhaps someone on here has, that points out the 
sharp increase in error rates found when a tech is used to gross, versus a 
trained pathologists' assistant. There is a drastic difference. It is distinct, 
and a study is really not needed to see that difference. Now, to be clear 
again, that is not to say that every tech that grosses does a bad job. No 
vitriol here. It is just a fact, and a troubling one at that.

Imagine the difference in quality you would see if you had me doing all your 
stains! I am not trained as an HT. You could argue that I could be trained, but 
do you really want to open that can of worms? Do you want medicine to allow for 
that, and risk the HT profession? Probably not, and we do not either.  Do you 
think I would be as good as you are, given all the real education you received 
when getting your HT training? I don't think I would be as good as you are at 
doing your job.

As an example to illustrate, anyone that grosses should know how to answer 
these very basic questions. These might help shed some light on the issue:

   1. What is the most common neoplasm of the gallbladder, what does it
   look like, and where is it found? Would you know it if you saw it? Is it
   benign, or malignant?
   2. What is the reason that all appendices should have the margin
   submitted in the initial submission?
   3. Would you know the difference between an esophageal bx and a bx from
   any other part of the GI tract simply by gross appearance? What would you
   do if you had 2 specimens, one esophagus and one duodenal and they were
   reversed in the specimen containers by the biopsy tech? Would you be able
   to pick up on that 

[Histonet] muscle biopsy stains ATPase

[Horn, Hazel V]

We are having a problem with our ATPase muscle stain.  Does someone out there 
have a good procedure for this?  And any tips or suggestions??
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org











**
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
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dissemination, distribution or copying of this communication is strictly 
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us immediately by replying to the message and deleting it from your computer.
Thank you.

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[Histonet] RE: Control Block Tracking, Part 2

[Horn, Hazel V]

Lucky lady

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hor...@archildrens.org
archildrens.org




100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, March 06, 2012 2:31 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Control Block Tracking, Part 2

And the count down continues. 18 days

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Tuesday, March 06, 2012 3:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Block Tracking, Part 2

Thank you to everyone for your tracking suggestions.  I think we all agree that 
there are reasonable regulations and requirements for QA, and we can also agree 
that some are doing nothing but making our jobs more complex when it's really 
unnecessary.  Soon, every laboratory professional will be attached to a tech 
robot - your clone physically!
Every time you make a move, the docutech photographs and documents.
At the end of the work day, you unplug your docutech and put him/her/it  in 
your locker for recharging.  I'm thinkin' there must be a movie in here 
somewhere - and it may not be a sci-fi feature.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] looking for rita humphrey

[Horn, Hazel V]

Rita would you contact me please?

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

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[Histonet] histotech needed

[Horn, Hazel V]

Arkansas Children's Hospital is looking for a registered histotech.  This is a 
dayshift, Monday-Friday position with rotating holidays on call.  Our lab 
performs routine HE's, special stains by hand and we have a Bond III 
immunostainer.  We process approximately 6000 surgicals a year.  ACH has 
excellent benefits.  Little Rock is nice small city in which to live.  We have 
many area attractions and if you like the outdoor life you will love Arkansas.  
  The position is posted as an MLT, histology lab.   Apply online at:
www.archildrens.orghttp://www.archildrens.org



Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

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[Histonet] job opening

[Horn, Hazel V]

Arkansas Children's Hospital has a position for a full time histology tech.   
This is a 8:30 am until 5:00 pm shift Monday through Friday.  We are a small 
friendly lab who works well together.  ACH offers excellent benefits.  Apply 
online at www.archildrens.orghttp://www.archildrens.org

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

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[Histonet] RE: Leica Bond verses the Ventana Ultra

[Horn, Hazel V]

We love our Bond too.  

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Friday, September 16, 2011 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra. 
 
judith_par...@memorial.org
 
Judith Pardue




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RE: [Histonet] Automatic HE stainer with coverslipper (glass)

[Horn, Hazel V]

We have a Leica stainer and coverslipper.  We like them both.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ernestine 
Middleton
Sent: Wednesday, August 31, 2011 8:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automatic HE stainer with coverslipper (glass)

Hi;
Looking and need comments on those of you that are using combination HE 
stainer with glass coverslipper.  
Thank you.
 
Ernestine Middleton, Manager,  HT, HTL ,BS ,MPA
Montefiore Medical Center
Bronx, New York
718-920-4157
emidd...@montefiore.org
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RE: [Histonet] knife used for cleaning paraffin off the blocks

[Horn, Hazel V]

We use a couple of butter knives we brought from home.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sris...@mail.holyname.org
Sent: Monday, August 08, 2011 8:29 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] knife used for cleaning paraffin off the blocks

Hi All,

I wonder if someone could help me with this!

I am looking for a stainless steel knife that we use to scrape off the 
paraffin from the embedded blocks.  I could not find the 6 inches size 
knife in any catalog. 

Thanks in advance

Mala

Nirmala Srishan
Histology Supervisor
Holy Name Medical Center.

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Holy Name Medical Center is the recipient of:

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Credentialing Center

100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern 
Healthcare

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Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. 
Power

Distinguished Hospital Awards for Clinical Excellence, HealthGrades

Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, 
HealthGrades

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RE: [Histonet] prostate bx containers

[Horn, Hazel V]

According to the CPT code book it is per specimen.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, July 11, 2011 2:54 PM
To: histonet@lists.utsouthwestern.edu; LauraJones
Subject: RE: [Histonet] prostate bx containers

Your pathologist is right. You cannot. Billing is PER block
René J.

--- On Mon, 7/11/11, Jones, Laura lpjo...@srhs-pa.org wrote:


From: Jones, Laura lpjo...@srhs-pa.org
Subject: RE: [Histonet] prostate bx containers
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Monday, July 11, 2011, 1:15 PM


We looked at these, but our Pathologist's first question was can we bill for 6 
or 8 bottles if they are technically all in one?  How is that handled?


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J 
[mjdess...@wvhcs.org]
Sent: Monday, July 11, 2011 11:44 AM
To: Sheila Adey; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] prostate bx containers

We don't currently use them but I've used them in the past.  As long as you 
don't 'bump' them on the grossing station while they're open, they're fine.  I 
have yet to see one leak.

Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care 
System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org  |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 
570-552-1526



From: Sheila Adey [mailto:sa...@hotmail.ca]
Sent: Sat 7/9/2011 9:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] prostate bx containers




Hello:

Has anyone used these prostate biopsy collection containers from Simport? It 
looks like a petri dish and has 12 individual wells in it, each one labelled 
with the location from the prostate.
We are going to trial them but I would like some opinions on them.
We currently get 10 to 12 bottles per case. So, all the bx's could go in this 
one container.
Thanks
Sheila


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RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper

[Horn, Hazel V]

As a manager I would never expect that kind of turn around.  I think it's 
impossible to achieve.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sgoe...@mirnarx.com
Sent: Monday, June 27, 2011 9:38 AM
To: rjbu...@yahoo.com; histonet-requ...@lists.utsouthwestern.edu; 
histonet@lists.utsouthwestern.edu
Subject: RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper

Another solution...get out of clinical and go into research =)  There are no 
quotas or slide per second expectations in the research world =)  So sorry you 
are having such a bad time with your job.  HT's are not a dime a dozen and 
usually it is fairly easy to find a better job.  Not to mention research pays 
almost double the clinical world!!
Good Luck!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Sunday, June 26, 2011 9:43 AM
To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cutper



---






Joanne:
Read the attachment so you can have an idea about productivity ranges and 
averages in different tasks in the lab.
If you embedded 214 blocks and cut 148  in 5.5 hours, using the averages I 
provided, embedding should have taken 3.5 hours and cutting 6.2 hours which 
means you worked 1.76 times FASTER than the expected average.
René J.

--- On Sat, 6/25/11, Joanne joanne0...@comcast.net wrote:


From: Joanne joanne0...@comcast.net
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
To: histonet@lists.utsouthwestern.edu
Date: Saturday, June 25, 2011, 7:07 PM


i am quite serious in my presentation and request for advice.  i too thought 
this goal was/is ridiculous to expect/ask for from someone so new and to attain 
in 6 months or less.  last monday i embedded 214 blocks and cut 148 between 5am 
and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for 
someone so very new i thought this pretty good . . . please note: most days 
aren't as hectic.  :)  what is an average though for blocks/minute?  what is 
meant by set sum per block? ---keeping in mind i am new to this field.



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[Histonet] RE: Leica CV 5030

[Horn, Hazel V]

We use Richard Allan Scientific mounting media with a 21 gauge needle.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Thursday, June 09, 2011 9:23 AM
To: Blazek, Linda; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica CV 5030

 Azer Toluene Based Mountant with the 21 needle

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda 
[lbla...@digestivespecialists.com]
Sent: Thursday, June 09, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica CV 5030

Anyone using the Lieca CV 5030, what mounting medium and what size needle are 
you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com

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RE: [Histonet] Submission of tonsils to pathology

[Horn, Hazel V]

Generally all are gross only.  There are some exceptions depending on patient 
history or if one is significantly larger than the other the pathologists have 
the discretion to do a microscopic. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, June 03, 2011 11:11 AM
To: Histonet; Richard Cartun
Subject: Re: [Histonet] Submission of tonsils to pathology

Labelled gross only.
René J.

--- On Fri, 6/3/11, Richard Cartun rcar...@harthosp.org wrote:


From: Richard Cartun rcar...@harthosp.org
Subject: [Histonet] Submission of tonsils to pathology
To: Histonet histonet@lists.utsouthwestern.edu
Date: Friday, June 3, 2011, 11:55 AM


What is your policy on the submission of tonsils to pathology for examination 
(gross or microscopic)?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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[Histonet] RE: Leica processor

[Horn, Hazel V]

We have one and it's been reliable.  It's about 5-6 years old.  It's also easy 
to use.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A
Sent: Wednesday, June 01, 2011 4:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Leica processor

Sorry, its an ASP300 processor.
Thanks

From: Bales, Candy A
Sent: Wednesday, June 01, 2011 4:05 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Leica processor

On behalf of a friend who is looking for a new tissue processor. She is asking 
for information on a Leica ASB300. Any information would be appreciated.
Also, any pros  cons of a long term lease/option to buy?

Thank you
Candy

Candy Bales, HT
Chief Histologist
The University  of Texas Dental Branch at Houston
Diagnostic Sciences-Oral Pathology
6516 M.D. Anderson Blvd. # 3.093
Houston, TX 77030
713.500.4411 office
713.500.4416 fax

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[Histonet] RE: printed requisitions

[Horn, Hazel V]

We still use paper reqs.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, June 01, 2011 3:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] printed requisitions


Does everyone still receive printed requisitions with your surgical specimens? 
How many of your OR's have a printer in each room? Our OR has a shared printer 
for all of the procedure rooms, but would like to have a printer for each OR 
suite. Our  IT department is saying there would be problems and has suggested 
that we go paperless. I see many problems arising from this. If anyone is doing 
this successfully, I'd be very interested in hearing how you accomplished it.


Toni

Regards,
Toni Rathborne
Pathology Supervisor
Somerset Medical Center
110 Rehill Ave.
Somerville, NJ 08876




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[Histonet] RE: embedding forceps

[Horn, Hazel V]

These are the same ones we like.  We like the 7 ones.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Monday, April 11, 2011 7:32 AM
To: Gale Limron; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding forceps

I like these:

You can get them in different lengths.5 1/2 or 7  

  
http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron
Sent: Monday, April 11, 2011 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding forceps

Good Morning!
Can anyone recommend comfortable, not-heated, embedding forceps (with ordering 
info)? Our favorite that we had for years disappeared a while back and we 
haven't found a replacement that we really like yet.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



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RE: [Histonet] Grossing Station Recommendation

[Horn, Hazel V]

We love our Gross Lab Sr.  ThermoShandon

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride
Sent: Monday, March 21, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Grossing Station Recommendation

Colleagues,

We are looking to replace our current pathology grossing hood with a new 
station, so I am looking for recommendations.  Thanks in advance for all of 
your wonderful advice.


Best regards,


~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-915-1683 (m)
412-268-8275 (fax)
smcbr...@andrew.cmu.edu 






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[Histonet] RE: glass coverslippers

[Horn, Hazel V]

We love our Leica CV 5030.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, March 17, 2011 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] glass coverslippers

I am in the market for a new coverslipper.  I am looking at Thermo's clearvue 
or Leica's CV 5030.  Does anyone have any input on either of these 
coverslippers, good or bad.
Thank you in advance,
Allison
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[Histonet] RE: microtome safety

[Horn, Hazel V]

I do the same as Tom.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Tuesday, March 08, 2011 8:50 AM
To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: microtome safety

Both.  Fingers to lift one end of the ribbon as I cut it and then with the 
other hand I use a small brush to lift the other end from the microtome.  
Allows me to stretch and smooth the ribbon as I lay it out on the water bath.

Some others here us forceps, picks, etc.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 08, 2011 8:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome safety

Morning all!

I need some quick responses to this question:  do you use your fingers or an 
instrument of some sort to pull your paraffin ribbons off the block when 
sectioning?  For those that do not use their fingers, what do you use?  If 
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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[Histonet] band saws

[Horn, Hazel V]

I am looking for a band saw to cut our bone tumors.  What do I need to be 
looking for?  Power?  Size?  Other suggestions?
Thanks!

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

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RE: [Histonet] procedural safeguards when accessioning identical sources

[Horn, Hazel V]

We use different colored cassettes. We have 6 different colors and rotate them 
with the cases.  The color of the cassette is also dictated as part of the 
gross description.   We use the same color of slides as the cassettes to carry 
this one step further.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Thursday, February 24, 2011 2:48 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] procedural safeguards when accessioning identical sources 

Our lab has the policy of not accessioning back to back specimens of the same 
source.  This helps us to ensure if there is a mix up the pathologist can tell 
when reading the case.  For example if the specimen source is a skin and they 
have an endocervical they would know.
We are soon to be getting a higher volume of prostates and will have to 
accession them together.
What kind of procedural safeguards do you have in place when working with a 
high volume of identical sources?

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cb...@lexclin.com



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RE: [Histonet] cdj

[Horn, Hazel V]

This is what I found at the CDC website:

Table 9. Tissue Preparation for Human CJD and Related Diseases
1. Histology technicians wear gloves, apron, laboratory coat, and face 
protection.
2. Adequate fixation of small tissue samples (e.g., biopsies) from a patient 
with suspected prion disease can be followed by post-fixation in 96% absolute 
formic acid for 30 minutes, followed by 45 hours in fresh 10% formalin.
3. Liquid waste is collected in a 4L waste bottle initially containing 600 ml 
6N NaOH.
4. Gloves, embedding molds, and all handling materials are disposed s regulated 
medical waste.
5. Tissue cassettes are processed manually to prevent contamination of tissue 
processors.
6. Tissues are embedded in a disposable embedding mold. If used, forceps are 
decontaminated as in Table 10.
7. In preparing sections, gloves are worn, section waste is collected and 
disposed in a regulated medical waste receptacle. The knife stage is wiped with 
2N NaOH, and the knife used is discarded immediately in a “regulated medical 
waste sharps” receptacle. Slides are labeled with “CJD Precautions.” The 
sectioned block is sealed with paraffin.
8. Routine staining:
a. slides are processed by hand;
b. reagents are prepared in 100 ml disposable specimen cups;
c. after placing the cover slip on, slides are decontaminated by soaking them 
for 1 hour in 2N NaOH;
d. slides are labeled as “Infectious-CJD.”
9. Other suggestions:
a. disposable specimen cups or slide mailers may be used for reagents;
b. slides for immunocytochemistry may be processed in disposable Petri dishes;
c. equipment is decontaminated as described above or disposed as regulated 
medical waste.
Handling and processing of tissues from patients with suspected prion disease 
The special characteristics of work with prions require particular attention to 
the facilities, equipment, policies, and procedures involved.10 The related 
considerations outlined in Table 9 should be incorporated into the laboratory’s 
risk management for this work.
288 Biosafety in Microbiological and Biomedical Laboratories
Table 10. Prion Inactivation Methods for Reusable Instruments
and Surfaces
1. Immerse in 1 N NaOH, heat in a gravity displacement autoclave at 121ºC for 
30 minutes. Clean and sterilize by conventional means.
2. Immerse in 1 N NaOH or sodium hypochlorite (20,000 ppm) for 1 hours. 
Transfer into water and autoclave (gravity displacement) at 121ºC for 1 hour. 
Clean and sterilize by conventional means.
3. Immerse in 1N NaOH or sodium hypochlorite (20,000) for 1 hour. Rinse 
instruments with water, transfer to open pan and autoclave at 121ºC (gravity 
displacement) or 134ºC (porous load) for 1 hour. Clean and sterilize by 
conventional means.
4. Surfaces or heat-sensitive instruments can be treated with 2N NaOH or sodium 
hypochlorite (20,000 ppm) for 1 hour. Ensure surfaces remain wet for entire 
period, then rinse well with water. Before chemical treatment, it is strongly 
recommended that gross contamination of surfaces be reduced because the 
presence of excess organic material will reduce the strength of either NaOH or 
sodium hypochlorite solutions.
5. Environ LpH (EPA Reg. No. 1043-118) may be used on washable, hard, 
non-porous surfaces (such as floors, tables, equipment, and counters), items 
(such as non-disposable instruments, sharps, and sharp containers), and/or 
laboratory waste solutions (such as formalin or other liquids). This product is 
currently being used under FIFRA Section 18 exemptions in a number of states. 
Users should consult with the state environmental protection office prior to 
use.
(Adapted from www.cdc.gov 11,12)
Working Solutions 1 N NaOH equals 40 grams of NaOH per liter of water. Solution 
should be prepared daily. A stock solution of 10 N NaOH can be prepared and 
fresh 1:10 dilutions (1 part 10 N NaOH plus 9 parts water) used daily.
20,000 ppm sodium hypochlorite equals a 2% solution. Most commercial household 
bleach contains 5.25% sodium hypochlorite, therefore, make a 1:2.5 dilution (1 
part 5.25% bleach plus 1.5 parts water) to produce a 20,000 ppm solution. This 
ratio can also be stated as two parts 5.25% bleach to three parts water. 
Working solutions should be prepared daily.
CAUTION: Above solutions are corrosive and require suitable personal protective 
equipment and proper secondary containment. These strong corrosive solutions 
require careful disposal in accordance with local regulations.
Precautions in using NaOH or sodium hypochlorite solutions in autoclaves: NaOH 
spills or gas may damage the autoclave if proper containers are not used. The 
use of containers with a rim and lid designed for condensation to collect and 
drip back into the pan is recommended. Persons who use this procedure should be 
cautious in handling hot NaOH solution (post-autoclave) and in avoiding 
potential exposure to gaseous NaOH; exercise caution during all sterilization 
steps; and allow the autoclave, instruments, and solutions to 

RE: [Histonet] High Complexity Testing

[Horn, Hazel V]

While the test is high complexity it is the READING of the test by the 
pathologist that determines its complexity.  Because histotechs do not report 
the results our part of this test is not high complexity.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, February 08, 2011 10:32 AM
To: histonet@lists.utsouthwestern.edu; Sheila Fonner
Subject: Re: [Histonet] High Complexity Testing

When a machine is doing the test, there are stringent provisions as to the 
preparation and validations of the test.
Done manually, it requires a trained technologists and, yes, they are high 
complexity tests (both IHC and FISH, and their variations).
René J.

--- On Tue, 2/8/11, Sheila Fonner sfon...@labpath.com wrote:


From: Sheila Fonner sfon...@labpath.com
Subject: [Histonet] High Complexity Testing
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, February 8, 2011, 7:45 AM


Hello All,



I would really appreciate it if anyone has information on whether IHC/ISH
are considered high complexity testing for histotechs.  Our pathologist
believes that ALL histology low complexity testing since a machine is
doing the work.  Can anyone help me out with some guidelines, literature,
etc. that says otherwise?  I would really appreciate it.  We just want to
know which one it is.



Thanks so much Histoland!







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[Histonet] RE: Physician Signatures

[Horn, Hazel V]

We have the physicians sign the reqs.  The surgeons sign them before the req 
goes to the chart and that copy is stored electronically in the EMR. We get a 
copy in our lab.  From our clinics the docs sign the reqs in clinic.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, December 17, 2010 10:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Physician Signatures


Is everyone concerned about getting a physician's signature on requisitions, or 
do you have a fool proof plan in place? Inquiring minds want to know... TIA!

How many of you have electronic ordering for AP?

This from CodeMap Compliance that I get..

Signatures Required on Laboratory Requisitions

Although many professional groups and organizations lobbied against the 
signature requirement, CMS is implementing this provision as part of the CY 
2011 MPFS. Starting January 1, 2011, the ordering physician must sign all 
written or paper requisitions for laboratory tests and services. Clinical 
laboratories will have to scramble to revise requisitions to include a 
signature line for the ordering physician.

However, those labs that receive test orders via electronic or web-based 
interfaces should not be affected by the signature requirement included in the 
2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states,

Our proposed policy does not concern electronic or telephonic requests, 
because we do not consider these types of requests to be requisitions. As we 
discussed previously, a requisition is the actual paperwork, such as a form 
that is provided to a clinical diagnostic laboratory that identifies the test 
or tests to be performed for a patient.



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


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[Histonet] RE: Eosin to dye small Biopsies

[Horn, Hazel V]

We put it in our 95%

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, October 21, 2010 3:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin to dye small Biopsies

Hello to all in histoland.  Are any of you using eosin on the processor
to dye your small bx's?  If so, are you putting it in the 100% alcohol
to do so?  Any help in this matter will be greatly appreciated.


Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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[Histonet] pathology reports

[Horn, Hazel V]

When you have results from an outside lab, i.e.  flow results, bone marrows, 
ect.  How do you integrate this into your path report?
It is a verbatim copy inside the report in the same format as the outside lab?  
Or can it be a copy with the results in a different format?  A discussion 
has occurred within in or transcription department over this matter.   We do 
attach the outside report to the hard copy report in our paper file.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

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RE: [Histonet] WS 50

[Horn, Hazel V]

I would love this same information.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara 
Baldwin/mhhcc.org
Sent: Wednesday, September 15, 2010 8:07 AM
To: Histonet
Subject: [Histonet] WS 50

HISTONETTERS
I was trying my best to get out to Seattle for the workshop Steve was doing on 
the lab design, our budget wouldn't allow it but any help I can get would be 
great we are going to start construction Jan 2011  We are getting 3 patient 
rooms and making them one big cytology/histology lab.  Is there a powerpoint or 
something out there to help me?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232, 
Pager 812-481-0897
Confidential information, Authorized use only.
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[Histonet] RE: Pathology billing for consultation

[Horn, Hazel V]

You cannot bill for consults if your pathologists requests them, is that 
correct?

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Wednesday, September 08, 2010 10:51 AM
To: 'Mahoney,Janice A'; 'Weems, Joyce'; Demarinis, Carolyn; 
histo...@pathology.swmed.edu
Subject: [Histonet] RE: Pathology billing for consultation

That is the case here also 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A
Sent: Wednesday, September 08, 2010 10:37 AM
To: 'Weems, Joyce'; Demarinis, Carolyn; histo...@pathology.swmed.edu
Subject: [Histonet] RE: Pathology billing for consultation

If our Pathologists need a second opinion we eat the charge.  If it is 
requested by the clinician, we request that he/she be billed.   If it is the 
patient we bill the patient (or ask the consultant to bill the patient)  All of 
this depends on patient status/medicare etc.
I'm very interested in seeing how others respond.
Jan Mahoney
Omaha

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Wednesday, September 08, 2010 9:46 AM
To: Demarinis, Carolyn; histo...@pathology.swmed.edu
Subject: [Histonet] RE: Pathology billing for consultation

It is my understanding that we cannot charge professional fees for other 
pathologists. Our hospital pays for the pathologists' consults if the patient's 
insurance will not or if the consultant does not bill third party.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Demarinis, 
Carolyn
Sent: Wednesday, September 08, 2010 09:22
To: histo...@pathology.swmed.edu
Subject: [Histonet] Pathology billing for consultation

I would like to know how other pathology labs are billing for consultations 
that are sent out by pathologist for second opinion.
Our process is to notify the physician's office that a case is being sent to an 
expert and, if required, the physician's office is responsible for obtaining 
precertification if the patient's insurance require it.
Unfortunately, this has caused us a number of problems.  If the consultant is 
not in-network, the insurance does not cover this expense, and the patient is 
responsible for the bill.
Is it a better option for the hospital to receive all bills from consultants, 
and in turn, the hospital will bill the patient?  If so, are there problems 
associated with this?
Or are other laboratories having the consultants bill the patient's insurance 
directly, and if so, are they experiencing similar problems?
Thanks.
Carolyn DeMarinis, Pathology Supervisor
Saratoga Hospital Laboratory




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[Histonet] bar coding specimens, slides, blocks

[Horn, Hazel V]

I am looking for a vendor that has the capability to barcode specimens, blocks 
and slides.   Also if it can interface with Meditech client server 6.0 it would 
be a plus.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

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RE: [Histonet] Will a Thermo Scientific Sales Representative please contact me

[Horn, Hazel V]

Ditto...I need the same help

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Wednesday, September 08, 2010 3:14 PM
To: 'Histonet'
Subject: [Histonet] Will a Thermo Scientific Sales Representative please 
contact me

I need to have a Thermo Scientific sales rep contact me to clarify ordering
Richard Allan staining reagents.  

 

Thank you

 

Gayle M. Callis 

HTL/HT/MT(ASCP) 

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RE: [Histonet] Excluded and Limited Tissues to Histo

[Horn, Hazel V]

I believe this list is generated by your surgical affairs medical staff.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax501.364.3155
 
visit us on the web at:www.archildrens.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
rick.garnh...@memorialhealthsystem.com
Sent: Wednesday, July 14, 2010 3:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Excluded and Limited Tissues to Histo


Does anyone know if CAP Today has an article within the last couple of
years as to what tissue can be excluded from submission and for gross
only
exams. The last article I have reference to was from 1996. Thanks


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnh...@memorialhealthsystem.com



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RE: [Histonet] Special Stain Procedures

[Horn, Hazel V]

For copper we use the kit from American Master Tech.  It works really
well.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax501.364.3155
 
visit us on the web at:www.archildrens.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
thisis...@aol.com
Sent: Tuesday, June 08, 2010 3:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stain Procedures


Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann



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RE: [Histonet] Special Stain Procedures

[Horn, Hazel V]

I have to disagree with Rene on this onethe Microwave Copper from American 
Master Tech is awesome and it's never failed.  It's a rhodamine stain and we 
don't even use the microwave.  We use a pressure cooker.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax501.364.3155
 
visit us on the web at:www.archildrens.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, June 09, 2010 8:02 AM
To: histonet@lists.utsouthwestern.edu; thisis...@aol.com
Subject: Re: [Histonet] Special Stain Procedures

Neither procedure is reliable. Use Tim's method (sulfur dioxide).
René J.

--- On Tue, 6/8/10, thisis...@aol.com thisis...@aol.com wrote:


From: thisis...@aol.com thisis...@aol.com
Subject: [Histonet] Special Stain Procedures
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, June 8, 2010, 4:43 PM



Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann



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