[Histonet] (no subject)

[Jamal Rowaihi via Histonet]

Hi colleagues We are going for Digital Pathology What is your recommendation 
What's the best device and company (Aperio, Philips, 3DHISTECH)

Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone
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Re: [Histonet] Marking Tissues with Eosin

[Jamal Rowaihi via Histonet]

Hi If you are using buffered Formaline so the Eosin color will not resist until 
the end of tissue processing.I recommend to add small amount of stock Eosin to 
the last alcohol in theTissue processor. 

Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone
 Original message From: Mca Werdler via Histonet 
 Date: 6/23/16  8:49 PM  (GMT+03:00) To: 
"Rebecca E. Ashley"  Cc: Histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Marking Tissues with Eosin 
Dear Rebecca,

Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.

Good luck,

Maarten

2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet@lists.utsouthwestern.edu>:

> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block.  I've heard mention of marking these with
> eosin to make them easier to see.  Has anyone done this?  Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
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Re: [Histonet] automatic stainers and tissue processors

[Jamal Rowaihi via Histonet]

Go for Sakura products with no hesitate. 


Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone Original message From: Silvia Bonner via 
Histonet  Date: 2/29/2016  11:09 PM  
(GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automatic 
stainers and tissue processors 

Hello,
We are looking into purchasing new automatic H strainers and new tissue 
processors.  Any helpful advice?  I know this may have been discussed before 
but I an new to histonet.
Thanks for your help!
Silvia Bonner, HT(ASCP) CM
Histology Supervisor

sbon...@pathregional.com
Pathologists' Regional Laboratory
1225 Highland Ave
Clarkston, WA 99403
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Re: [Histonet] Lead Tech/ Supervisor Competency

[Jamal Rowaihi via Histonet]

Thanks FawanI think some are not completely satisfied with their papers that's 
why they didn't respond to us, but I think we suppose to share our experience 
to improve the quality, soon I will send you my own and I hope you send me 
yours... Good luck. 


Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone Original message From: Fawn Bomar via 
Histonet  Date: 2/19/2016  5:36 PM  
(GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lead 
Tech/ Supervisor Competency 
Hi all,



I didn't receive any responses in regards to my last post so I thought I would 
try one more time.  Does anyone have a competency or orientation checklist that 
they use for lead techs or supervisors that they would be willing to share?  On 
my last post, everyone that messaged asked for copies of my assessment and the 
assessments that were sent to me, which I did send to everyone that requested 
them.  If anyone else would like me to send my assessment to the them to look 
at, please let me know and I will be happy to share, plus I would be 
appreciative of any feedback- positive or negative- and any suggestions for 
improvement.  I tried to send an attachment of my assessment to the histonet 
but it kept saying it was waiting for moderator approval and it never was sent.



Thank you all for your help and guidance.



Fawn
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Re: [Histonet] Nuclear Bubbling

[Jamal Rowaihi via Histonet]

Great, I agree 


Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone Original message From: "Manfre, Philip via 
Histonet"  Date: 2/16/2016  10:44 PM  
(GMT+03:00) To: Rene J Buesa , "Vickroy, James" 
 Cc: histonet@lists.utsouthwestern.edu Subject: 
Re: [Histonet] Nuclear Bubbling 
Sort of a rude response to someone looking for help.

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, February 16, 2016 1:12 PM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear Bubbling

If I remember correctly, this issue has been discussed previously.The general 
consensus as to the cause of nuclear "bubbling" (in reality a lack of staining 
in the nuclear area) has been attributed to an incomplete section drying.After 
the section has be "fished" from the water bath, if the slide is not set to 
drain the underneath water before drying, the nuclear components are dissolved 
hence when the section is stained, there is nothing to stain → "nuclear 
bubbling".I think this has been previously stated so I really do not understand 
posting this same question again.I do not think that posting again the question 
a different answer is going to be received.rené 

    On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" 
 wrote:
 

 
Struggling to find an answer.  We do a lot of GI biopsies in our lab.  
Sometimes they look wonderful without any nuclear bubbling, other times the 
bubbling is pretty intense.  Since nuclear bubbling is often attributed to 
incomplete fixation we of course have investigated the fixation times.  I do 
not find that the problem is fixation.  In fact some of the biopsies end up 
fixing for 48 hrs before processing. (weekend).  There was a suggestion last 
week or so that there might be water trapped under the slides after cutting and 
before staining.  I really thought that this might be the issue however I'm not 
sure at this point.  Extra drying seems to help but sometimes slides side by 
side are so variable, one with bubbles and one without.  I also don't believe 
the problem is in the processing schedule since the problem has shown up on 
both a rapid and a normal schedule. (therefore longer dehydration, clearing, 
etc.)

I am wondering if anyone else has worked with this issue.  Here are my 
questions:


1.        Could it be something that is happening with the tissue before it 
gets to the lab?  Usually a delay if fixation  causes other artifacts but not 
bubbling.  Could it be heat from the GI procedure?

2.      We do use blue sponges for our biopsies.  I know some say get rid of 
the sponges but has anyone seen this problem caused by usage of sponges?

3.      What about the heat stage in our Prisma stainer?


I am really getting frustrated.  Pathologists never complain however I would 
rather all of the tissue did not have the "nuclear bubbling".  Again we only do 
biopsies so I really don't think the standard old " not enough time in 
formalin" is the issue.  I have even wondered about variables such as we use 
recycled formalin, recycled Clearite III.

Any suggestions?

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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Re: [Histonet] PAP stain quality

[Jamal Rowaihi via Histonet]

You need to review the slides fixation and what chemicals you are using


Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone Original message From: Charles Riley via 
Histonet  Date: 2/4/2016  5:50 PM  
(GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAP stain 
quality 
Not sure if anyone out the would know the answer to this. We are having an
issue with our PAP stained slides appearing too orange and look aged. If
you have any idea for causes I appreciate any help

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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