[Histonet] Histotech Opportunity in South Georgia - SEPA Labs

2023-05-24 Thread Jennifer Johnson McIntyre via Histonet
SEPA Labs, since 1992, is a dynamic CAP accredited laboratory serving multiple 
regional facilities in Georgia, South Carolina, and Florida.  We are committed 
to providing superior quality anatomical pathology services to our patients and 
physicians, while exceeding service expectations through our dedicated 
employees.

SEPA Labs is currently seeking an experienced Histotechnician for a full-time 
night shift opportunity.  The histotechnician will prepare histologic slides 
from tissue sections for microscopic examination and diagnosis by a 
pathologist.  The candidate needs to possess a broad knowledge of the technical 
aspects of histology including but not limited to section cutting, embedding, 
frozen section, and special staining.

This position is onsite at our main laboratory in Brunswick, Georgia.  
Brunswick is situated in Glynn County on the southern portion of Georgia's 
scenic coastline and is the gateway of the Golden Isles.  Glynn County is 
comprised of 4 barrier islands - St. Simons Island, Jekyll Island, Sea Island, 
and Little St. Simons Island - and the mainland containing the city of 
Brunswick.  Brunswick is separated from the barrier islands by the beautiful 
Intracoastal Waterway.

Whether you're looking for serene marshes, coastal wildlife, breathtaking 
beaches, world-class golf and fishing, historical landmarks, family activities, 
quaint shopping, festivals or galleries, the Golden Isles has something for 
everyone.

Learn more about SEPA at www.sepalabs.com<http://www.sepalabs.com>

For more information or to apply, please visit the careers section of the SEPA 
Labs website or contact Jennifer McIntyre, 
jjohn...@sepalabs.com<mailto:jjohn...@sepalabs.com>.



Jennifer (Johnson) McIntyre, SHRM-CP
HR Manager, SEPA Labs
Phone: (912) 261-2669, ext. 1059
Fax: (912) 261-0753

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[Histonet] Chatter in sections

2023-01-21 Thread Jennifer Johnson via Histonet
We recently switched paraffin and our pathologist is complaining of chatter in 
sections.  My coworker and I split blocks and some sections are cut by her and 
some by me.  The only difference we have made is a change in paraffin   
paraffin.  Any suggestions for a smoother section?   Just for informational 
purposes, the old paraffin used was Henry’s wonder wax which has been 
discontinued and the new paraffin is Surgipath EM 400.  
Thanks, Jennifer

Sent from Jenny's iPhone
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[Histonet] Is there a Pathology billing hotline?

2014-05-30 Thread Jennifer Johnson
Today we received one container labelled 'sentinel nodes' for frozen section. 
There were five nodes and they were placed in two chucks. How in the heck are 
we to know how to code for charges for this?  Is it per container, per node, or 
per frozen?  Plus, is there a person or agency that you can call to ask these 
questions?
Thanks, Jennifer Johnson

Sent from Jenny's iPhone
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[Histonet] Local service company for cryocut 1800 in San Diego area?

2012-10-01 Thread Jennifer Johnson
Hi Guys

Can anyone recommend a local service company for the cryocut 1800 in the San 
Diego area. Thanks in advance. Jennifer

Jennifer L. Johnson, Ph.D.
Staff Scientist
The Scripps Research Institute


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[Histonet] Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome?

2012-08-07 Thread Jennifer Johnson
Hi Guys

Does anyone have a copy of the manual for the Reichert-Jung/Leica Cryocut 1800 
Cryostat with 2020 microtome?  Thanks in advance.

Jennifer L. Johnson, Ph.D.
Staff Scientist
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla, CA 92037
jjohn...@scripps.edu



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[Histonet] Histology for kids

2009-07-22 Thread Jennifer Johnson

I did tonsils one time and since most of them have had theirs removed, they 
were very interested.  We also have autopsy tissue, brains, lung, liver, heart, 
in jars of formalin which are always a hit for them to see but not touch!  Good 
Luck, it will be so much fun watching their faces.

 

Jennifer Johnson, HTL (ASCP)

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[Histonet] Eosin in Alcohol

2009-07-14 Thread Jennifer Johnson

A couple of weeks ago I posted the message below on the histonet and all of you 
responded that it shouldn't matter so I have finally gotten a reply from the 
company we send our prostate biopsies off to and below is their response.  So 
now you know the rest of the story!

 

We have used Eosin in the last 95% alcohol on the tissue processor for several 
years. I usually add approximately 5 ml to the full jug. It is a great tool to 
use for embedding. However, we received a letter from the lab that we send our 
prostate biopsies to saying that it was undesirable because it interfered with 
their immuno staining. They sent us some cobalt blue to use in the place of 
eosin along with mixing instructions and the whole batch of tissues came out 
such a dark blue. There is no delineations in the color of the blue and I found 
it to be useless for helping to embed. I would rather do without anything than 
use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool 
to use unless you are doing immunos on prostate biopsies. 
 
Thanks,
 
Jennifer Johnson, HTL (ASCP) 


Their reply:  The problem is that eosin belongs to a family of polycyclic 
aromatic flourescent compounds that in high concentrations binds to and 
saturates all tissue  components.  When immunoflourescence is performed on such 
tissue- as in the prostate px+ test- the diffuse background autoflourescence 
signal from prior treatment with these compounds can interfere with, and even 
totally overwhelm, the signal of the flourescent-labeled antibodies used to 
localize biomarkers in the tissue.

 

 

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[Histonet] Re: Eosin in alcohol

2009-07-01 Thread Jennifer Johnson

We have used Eosin in the last 95% alcohol on the tissue processor for several 
years.  I usually add approximately 5 ml to the full jug.  It is a great tool 
to use for embedding.  However, we received a letter from the lab that we send 
our prostate biopsies to saying that it was undesirable because it interfered 
with their immuno staining.  They sent us some cobalt blue to use in the place 
of eosin along with mixing instructions and the whole batch of tissues came out 
such a dark blue.  There is no delineations in the color of the blue and I 
found it to be useless for helping to embed.  I would rather do without 
anything than use cobalt blue.  I guess the point of my rambling is, Eosin is a 
wonderful tool to use unless you are doing immunos on prostate biopsies.  

Thanks,

Jennifer Johnson, HTL (ASCP) 

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[Histonet] (no subject)

2009-05-01 Thread Jennifer Johnson

Can anyone tell me how to use liquid nitrogen on fatty sections and also give 
me a source for purchasing it and a rough estimate of how much it costs.  I 
would appreciate any help.  We have been battling difficult breast specimens at 
our tiny little (cheap) hospital for entirely too long. 

 

Thanks,

 

Jennifer Johnson, HTL,(ASCP)

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