[Histonet] Cryostat sectioning of frozen leaves

[Jo-Ann Bader, Ms. via Histonet]

I am trying to cut frozen leaf sections at 6 and 8 microns.  All I am getting 
is bunched up leaf.  What is the trick/  I am cutting at -20 should be cutting 
at a lower temp and cutting thicker?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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Re: [Histonet] Histonet Digest, Vol 151, Issue 18

[Jo-Ann Bader, Ms. via Histonet]

We are happy the ABCAM CD3


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: June 21, 2016 12:00:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 151, Issue 18

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Today's Topics:

   1. CD3 antibody for FFPE mouse tissue (Connolly, Brett M)
   2. Re: CD3 antibody for FFPE mouse tissue (Elizabeth Chlipala)
   3. Re: CD3 antibody for FFPE mouse tissue (Connolly, Brett M)


--

Message: 1
Date: Tue, 21 Jun 2016 09:59:58 -0400
From: "Connolly, Brett M" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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for affiliates is available at
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not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
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--

Message: 2
Date: Tue, 21 Jun 2016 09:19:49 -0600
From: Elizabeth Chlipala 
To: "Connolly, Brett M" ,
"histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:
<14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Brett

Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for 
a CD3 that only detects mouse?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Connolly, Brett M via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 21, 2016 7:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD3 antibody for FFPE mouse tissue

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.