Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

[Bartczak, Joanna via Histonet]

I have seen this phenomenon years ago on the Bond III when I worked at a 
different laboratory.  If I remember correctly, Leica had to replace the 
syringes and lines as air bubbles were being introduced when aspirating 
reagents.
I would definitely contact Leica technical service and let them investigate.  
Good luck.

Sincerely,
Joanna


Joanna Bartczak

Charge Technologist - Immunopathology
University Health Network - Toronto General Site

200 Elizabeth Street

Toronto, Ontario M5G 2C4

Phone:  (416) 340-4800 ext.6004

joanna.bartc...@uhn.ca



From: Paula Keene Pierce 
Sent: Wednesday, August 12, 2020 6:42 PM
To: Morken, Timothy ; Histonet 

Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

I have seen this when using Sequenza cover plates.
My thoughts were that it is dissolved gases in the washes. As I noticed that 
when filling a container with my tap water and leaving it a while, bubbles form 
on the sides.
Do you brush away bubbles in your water bath even when using distilled water 
too?
Try not using freshly made, stirred, agitated, etc. solutions and adding a 
surfactant.
Extreme solution: place solutions in a vacuum.


Sent from Smallbiz Yahoo Mail for iPhone


On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet 
 wrote:

Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue 
sections that cause unstained (ie, clear) areas where bubbles have formed?

We've been trying things like extra buffer wash before reagent, new slide trays 
(tech rep suggestion). It is seen on single- antibody slides and very 
noticeable on dual - stain slides on which the first stain is there but the 
second stain shows numerous bubbles that do prevent the second stain from areas 
of the tissue.  We can see the bubble artifact on the slide away from tissue 
sections as well.

Any clues to solve this will be appreciated!
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] Canadian histology work

[Bartczak, Joanna via Histonet]

Hello Alicia,


In Canada you must pass the CSMLS exam.  If you look up their website 
(CSMLS.org), you will be able to find all the relevant information, under First 
Steps to Certification.

Also, in Canada, "histotechs" are medical laboratory technologists.


Good luck.

Joanna


Joanna Bartczak

Charge Technologist - Immunopathology
University Health Network - Toronto General Site

200 Elizabeth Street

Toronto, Ontario M5G 2C4

Phone:  (416) 340-4800 ext.8037

joanna.bartc...@uhn.ca



From: Alicia Murua 
Sent: Thursday, August 15, 2019 4:56 PM
To: Histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Canadian histology work

Hello,
Is anyone knowledgeable about American ASCP HT certificates and Canadian
Histotech requirements? If an American wanted to work in Canada what would
be the requirements?


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Re: [Histonet] TMArrayer: Pathology Devices

[Bartczak, Joanna via Histonet]

Hello,


I am looking to purchase new punches for the TMArrayer and am not having any 
luck contacting anyone at Pathology Devices (Ron).

Does anyone out there in Histoland have current contact information?  Help!


Thanks in advance,

Joanna


Joanna Bartczak

Charge Technologist - Immunopathology
University Health Network - Toronto General Site

200 Elizabeth Street

Toronto, Ontario M5G 2C4

Phone:  (416) 340-4800 ext.8037

joanna.bartc...@uhn.ca

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Re: [Histonet] Porcessing FFPE tissue without alcohol??

[Joanna via Histonet]

How about Sudan Black stain? 

> On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet 
>  wrote:
> 
> The only problem I see is that the fat will be preserved, as you wrote, as a 
> black osmium oxidate but you will not be able to use any "standard" fat 
> stain; otherwise it will work.René 
> 
>On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" 
>  wrote:
> 
> 
> 
> 
> Fix the tissue in Formalin, wash well in dw, then place very small pieces 
> into Osmium tetroxide solution ( std soln for TEM post-fixation)
> Processing to Pwax as usual.
> Basically, you will see lipids as black ( oxidised osmium)
> That's the only way to demonstrate solvent- soluble lipids, using Pwax 
> processing.
> Sure, there are caveats but, in the main...it will be Ok, imho.
> I invite comments as I may be doing exactly this very soon, to count 
> myelinated nerve fibres in a sciatic nerve.
> 
> 
> 
>   
> 
> Carl Hobbs FIBMS 
> Histology and Imaging Manager 
> Wolfson CARD 
> Guys Campus, London Bridge  
> Kings College London 
> London 
> SE1 1UL 
>   
> 020 7848 6813
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[Histonet] SDH

[Joanna via Histonet]

Anybody willing to share a good SDH protocol for fresh frozen muscle biopsy. 
Thanks for any help. 

Joanna 

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Re: [Histonet] Anti-roll plate

[Joanna via Histonet]

We have a Leica CM 1850. The anti-roll plate has a metal frame around it. I 
tried Leica and they said they don't make them anymore in Germany. But I'll try 
to contact Avantik.

Thanks 
Joanna



 On Aug 12, 2015, at 6:50 AM, Anne Murvosh amurv...@advancederm.net wrote:
 
 Avantik Biogroup sells the Leica anti-roll plate it's item #5260164 their # 
 is 1-888-392-8411.  They cost about $130.00. it just says 70mm w. If that's 
 not the right size (I have a newer leica cm1850) try Leika themselves.  Anne
 
 -Original Message-
 From: Joanna via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
 Sent: Tuesday, August 11, 2015 5:46 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Anti-roll plate
 
 I'm looking for a glass anti-roll plate (8 x 3 cm) for an old Leica cryostat 
 with old reusable steel blade. 
 
 Thanks. 
 
 Joanna 
 
 
 
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[Histonet] Anti-roll plate

[Joanna via Histonet]

I'm looking for a glass anti-roll plate (8 x 3 cm) for an old Leica cryostat 
with old reusable steel blade. 

Thanks. 

Joanna 



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[Histonet] use of denatured alcohol

[Joanna Bartczak]

Hello,

Our lab is considering switching to denatured alcohol as a cost saving 
initiative.
Is  anyone using denatured alcohols and performing subsequent Class II IHC 
testing with no impact to results?

Thank-you,
Joanna

Joanna Bartczak
MLT II - Immunohistochemistry
Calgary Laboratory Services
403-770-3695



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[Histonet] use of denatured alcohol

[Joanna Bartczak]

Hello,

Our lab is considering switching to denatured alcohol as a cost saving 
initiative.
Is  anyone using denatured alcohols and performing subsequent Class II IHC 
testing with no impact to results?

Thank-you,
Joanna

Joanna Bartczak
MLT II - Immunohistochemistry
Calgary Laboratory Services
403-770-3695



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[Histonet] Gomori Trichrome

[Joanna]

Hi, I was hoping you guys can help us with our problem with modified gomori 
trichrome stain for frozen muscle biopsy. We've been seeing a lot of red 
blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R 
dyes, including changing our recipe. So far no success. 

Hopefully  you can give us some suggestions. 

Thanks. 

Neuropathology lab
Vancouver, Canada 



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