[Histonet] Processor Preferences??
Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue processors
Hello Histoworld, What processors are people using to process bone or soft tissue samples for plastic? Do any of your processors allow for use of the monomer in the processor before embedding? What are your opinions, pro or cons, on the Tissue- Tek VIP5 or 6, the Leica ASP300, or the Thermo Pathcentre units? Hearing yours experiences in using them is greatly appreciated! My best, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] destaining bone
Hello All, I hope someone can assist us with a protocol for destaining some mouse ulna. A student stained his bones for 12 hours in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and then 100% as a prestain then into 100 % ethyl alcohol to destain some prior to pmma plastic embedding and sectioning to look at microdamage in the cortical bone. The bones are over stained and if possible we would like to destain the bone and continue on without getting more specimens. Any suggestions are welcome. Thank you in advance. John Baker and Mathieu Davis John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
