Re: [Histonet] Vendor with all items needed/ Histonet Digest, Vol 182, Issue 3

[John O'Brien via Histonet]

Hi Heather,
 Please contact IMEB INC for pricing and service on all your pathology
supplies, chemical needs and Pathology instruments.
We will provide the service you require to keep cost down,
Contact tra...@imebinc.com
Or   sa...@imebinc.com
Web site  www.imebinc.com
Regards,
IMEB INC

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Thursday, January 03, 2019 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 182, Issue 3

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Today's Topics:

   1. IHC Negative reagent controls (Cayman Fleck)
   2. Re: IHC Negative reagent controls (Tony Henwood (SCHN))
   3. AM to statlab and recommendations (Heather Marlatt)
   4. Re: IHC Negative reagent controls (John Garratt)
   5. IHC-H HELP... (Curt)


--

Message: 1
Date: Thu, 3 Jan 2019 02:48:10 +
From: Cayman Fleck 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] IHC Negative reagent controls
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

A question that came up regarding negative reagent controls for
IHC...currently using Ventana i-View.  Our regular negative control goes
through the standard antigen retrieval steps, like 99% of our antibodies.
However there are a small number of antibodies that require enzyme as well
(Protease 1).  I've seen a number of suggestions regarding this for the
negative reagent control...some say use an additional negative control
protocol that includes the protease, some say to use a single negative
control protocol and just include the harshest cell conditioning that any of
your protocols use (so basically use the cell conditioning + protease
negative control for all antibodies)...i-View is not polymer-based so we
need to continue using negative controls.  Any thoughts or advice?

Frank

Sent from Outlook


--

Message: 2
Date: Thu, 3 Jan 2019 04:06:57 +
From: "Tony Henwood (SCHN)" 
To: Cayman Fleck 
Cc: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] IHC Negative reagent controls
Message-ID:
<0e5c3ac509b7424e942160149bb36...@svdcmbx-mex024.nswhealth.net>
Content-Type: text/plain; charset="us-ascii"

Hi Cayman,

Unfortunately, applying HIER to a negative control for an antibody that
requires enzyme retrieval (or no retrieval at all) is not appropriate.
The pre-treatment processes are different and could unmask different
epitopes.
If you are using a negative control then the whole procedure needs to be
same with the exception or replacing the localisation antibody with an
Isotypic antibody solution. (Isotype controls are primary antibodies that
lack specificity to the target, but match the class and type of the primary
antibody used in the application.)

For example, applying citrate or EDTA HIER to sections prior to using the
CD99 antibody (clone 12E7) can reveal perinuclear (golgi-like) staining of
some tumours (eg some colonic carcinomas) but this is not seen if enzyme
retrieval is used.

Hope this is useful

Regards 

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow,
School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: Cayman Fleck via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 3 January 2019 1:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Negative reagent controls

A question that came up regarding negative reagent controls for
IHC...currently using Ventana i-View.  Our regular negative control goes
through the standard antigen retrieval steps, like 99% of our antibodies.
However there are a small number of antibodies that require enzyme as well
(Protease 1).  I've seen a number of suggestions regarding this for the
negative reagent control...some say use an additional negative control
protocol that includes the protease, some say to use a single negative
control protocol and just include the harshest cell conditioning that any of
your protocols use (so basically use the cell conditioning + protease
negative control for all 

Re: [Histonet] Pathology Instruments Histonet Digest, Vol 180, Issue 21

[John O'Brien via Histonet]

IMEB INC sells all the Pathology equipment you have interest in, We prefer
to sell the Sakura or Leica stainer because they are the most reliable we
find after 30 years of pathology Service
Regards,
IMEB INC

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Monday, November 26, 2018 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 180, Issue 21

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Today's Topics:

   1. Stainer Quotes (Charles Riley)


--

Message: 1
Date: Mon, 26 Nov 2018 11:52:47 -0500
From: Charles Riley 
To: Histo List 
Subject: [Histonet] Stainer Quotes
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Are there any vendors out there selling any of the following refurbished
item?

1. Leica ST5010  or ST5020 stainer
2. Sakura DRS 2000
3. DiaPath Giotto
4. Shur/Stain 3030


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


--

Subject: Digest Footer

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End of Histonet Digest, Vol 180, Issue 21
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Re: [Histonet] Histonet Digest, Vol 177, Issue 16

[John O'Brien via Histonet]

Decal Cal Stat company was sold to Stat Lab Medical Products. Last year

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Friday, August 17, 2018 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 177, Issue 16

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Today's Topics:

   1. FW: Histotechnologist, nights, Saturday thru Thursday, UCSF
  Medical Center, San Francisco, CA (Morken, Timothy)
   2. Recall: Histotechnologist, nights, Saturday thru Thursday,
  UCSF Medical Center, San Francisco, CA (Morken, Timothy)
   3. Unstained slides - how long are they good for? (P Sicurello)
   4. Re: Unstained slides - how long are they good for? (Jamie Watson)
   5. Re: Unstained slides - how long are they good for?
  (Tony Henwood (SCHN))
   6. Decalcification for Bone Marrow Biopsies (Cartun, Richard)
   7. Re: Unstained slides - how long are they good for?
  (Morken, Timothy)


--

Message: 1
Date: Thu, 16 Aug 2018 22:12:38 +
From: "Morken, Timothy" 
To: Histonet 
Subject: [Histonet] FW: Histotechnologist, nights, Saturday thru
Thursday, UCSF Medical Center, San Francisco, CA
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"


A position is open for an night-shift histotech at UCSF, San Francisco.
Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and
1:00 AM

The Histology Lab at UCSF is a full service lab with routine, special
stains, IHC and ISH. The workflow is fully barcoded and all equipment is
state of the art. 

Pay ranges (hourly, depending on experience) with night shift differential
(to be confirmed by HR on offer):

Histotech 1:?? $41.07 to 51.16, $3.45
Histotech 2:? 44.09 to 54.91, $4.50
Histotech 3:? 48.16 to 60.07, $4.50

UCSF also offers extensive benefits:?
http://jobs.ucsfmedicalcenter.org/whychoose_employment.html


Apply at:
http://jobs.ucsfmedicalcenter.org/

Job ID is 16324

Contact Yuri Murphy, Histology Supervisor, ?at ?yuri.mur...@ucsf.edu with
any questions.


Job Title

Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3) Job
ID  16324

Job Code   9065, 9066, 9067 
Job Family,  Technical & Technologist
Location:   Mount Zion (SF)
Weekly Hours:   40 :? 100%
Appointment Type:   Career
Department:  Pathology-Surgical/Histology Shift"  8-hour Nights Full/Part
Time:  Full-Time


Union Information:   This classification is represented by a union


Favorite Job


At UCSF Health, our mission of innovative patient care, advanced technology
and pioneering research is redefining what's possible for the patients we
serve - a promise we share with the professionals who make up our team.?
Ranked by U.S. News & World Report as the number one hospital in California
- and among the top five in the country - UCSF Health is committed to
providing the most rewarding work experience while delivering the best care
available anywhere. In an environment that allows for continuous learning
and opportunities for professional growth, UCSF Health offers the ideal
atmosphere in which to best use your skills and talents.

Job Summary


Classification of Histotechnologist 1,?2, or 3 will be determined based upon
the qualifications of the selected candidate.
Under supervision (HT-I, II level) by Senior-level technologists, Lead
technologist and the Histology Supervisor, or direction (HT-III) by Lead
technologist and the Histology Supervisor, or direction (HT-Lead) by the
Histology Supervisor, the incumbent serves as a Histotechnologist in the
Histology laboratory.
Duties include tissue processing, embedding, paraffin sectioning, H
staining, Special Staining, specimen receipt and accessioning, Laboratory
information system operation, Quality Assurance record keeping, instrument
maintenance, intra-operative frozen sections, and other technical duties as
assigned, including coverage in the Immunohistochemistry laboratory and
Grossing lab as determined by the Lab Manager.
Rotates weekly between workstations within the lab. Work schedule is
variable to include Saturdays and holiday coverage as scheduled. Incumbent
must be able to flex work hours as needed to meet department operational
needs and cover work rotations.

Required Qualifications


. HT 1:
o Associate degree?or at least 60 semester hours of academic credit from a
regionally accredited college/university, with a 

Re: [Histonet] What type saw? Histonet Digest, Vol 173, Issue 13

[John O'Brien via Histonet]

Hi Sandy, 
   My company sell many different type saws, ?Are you looking for an Autopsy
Saw ,like Stryker saws or  table top band saws.
We have  both available/
Regards,
John
IMEB INC

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Wednesday, April 18, 2018 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 173, Issue 13

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When replying, please edit your Subject line so it is more specific than
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Today's Topics:

   1. Autopsy Saws (Sandra Cheasty)
   2. Re: Autopsy Saws (Michael Gudo (Morphisto GmbH))


--

Message: 1
Date: Tue, 17 Apr 2018 19:54:57 +
From: Sandra Cheasty 
To: "Histonet (histonet@lists.utsouthwestern.edu)"

Subject: [Histonet] Autopsy Saws
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Hello all,
We need to replace two of our Mopec saws. We perform
necropsies on many large species, (bovine, draft horses, zoo animals), and
are looking for a vendor who can give us a deal on the purchase of two
sturdy autopsy saws that will stand up to heavy use. We are a veterinary
teaching hospital, and our budget is limited.
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine



--

Message: 2
Date: Wed, 18 Apr 2018 07:45:16 +0200
From: "Michael Gudo (Morphisto GmbH)" 
To: Histonet@lists.utsouthwestern.edu
Cc: Sandra Cheasty ,   Adrian Beuthner
, Linda Durbin

Subject: Re: [Histonet] Autopsy Saws
Message-ID: 
Content-Type: text/plain;   charset=utf-8

Hello Sandra,

if you need very high quality pathology saws we recommend the machines from
EXAKT. We have three of them in our lab and use them for pathology
sectioning as well as for cutting grinding techniques. They work with a
diamond band and there are several kinds of diamond bands available.

The website of EXAKT you can find here:
http://exaktusa.com/pathology-equipment/

You can contact Linda Durbin (linda.dur...@exaktusa.com), I think she can
give you a local contact.

With best regards
Michael

> Am 17.04.2018 um 21:54 schrieb Sandra Cheasty via Histonet
:
> 
> Hello all,
>We need to replace two of our Mopec saws. We perform
necropsies on many large species, (bovine, draft horses, zoo animals), and
are looking for a vendor who can give us a deal on the purchase of two
sturdy autopsy saws that will stand up to heavy use. We are a veterinary
teaching hospital, and our budget is limited.
> Cheers!
> Sandy
> 
> Sandra J. Cheasty, HT (ASCP)
> Histology & Necropsy Supervisor
> UW-Madison, School of Veterinary Medicine
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weism?llerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz:
DE243397199


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