Re: [Histonet] paraffin sectioning possibly calcified heart valves
Have you tried using RDO on the block surface? On Aug 12, 2015 12:30 PM, Jennifer Connell via Histonet histonet@lists.utsouthwestern.edu wrote: Hello, I am having trouble with sectioning of paraffin embedded human aortic valve samples. I received the embedded samples from a collaborator who had the embedding done by the pathology department at her institution. Six of the 12 samples sectioned fine but the remaining samples are developing ribbon cuts and/or are crumbling in areas when sectioned. The suggestion that calcification is causing the issue has been raised by other members of my lab. Some of the samples do appear to have calcific nodules while others may have a small bit of calcification along the valve. The samples with what appears to be calcific nodules do feel stiff at the nodules and are not being cut flat with the rest of the sample there. If I reposition the samples to a new part of the blade, the defects (ribbons or crumbling) are still in the same place or reappear in the same place quickly. I use Tissue-Tek Accu-Edge disposable high-profile microtome blades on a Lieca RM 2135 microtome. For the samples that do not have the obvious nodules - is it possible this was an embedding error and I may be able to re-embed the samples and proceed? If calcification is the issue - is it possible to de-calcify the valves after they have already been fix, dehydrated, and embedded? Or are there any other suggestions on how I could proceed? Any advice or suggestions would be greatly appreciated. Thanks. Jen -- Jennifer Petsche Connell, Ph.D. Lab Manager and Research Scientist Integrative Matrix Mechanics Laboratory http://www.proteoglycan.com/ Department of Bioengineering Rice University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Handline paraffin
Is there any written documentation? On Aug 3, 2015 11:10 AM, Rene J Buesa via Histonet histonet@lists.utsouthwestern.edu wrote: It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or irritating substance. It is just an oil of high molecular weight (mineral oil)René On Monday, August 3, 2015 12:48 PM, Johnson, Carole via Histonet histonet@lists.utsouthwestern.edu wrote: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Our Histology laboratory currently uses Mercurochrome to mark specimens, but we need to discontinue using it. Can anybody tell me of a good substitute. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
