We just recently went through a similar problem with irregular staining,
light staining, sometimes no stain of any kind including
counterstain,tissue falling off, and a handfull of false negative
patient tissues, but positive controls (on the same slide). With two
XT's running continuously it taken several days and everyone's help
isolating the problemnot to mention several different lots numbers
of slidesyikes! Next time I'll blame the slides right off the bat.
Kari L. Bradshaw HT(ASCP)
Laboratory Manager
Lower Columbia Pathologists
(360)425-5620
kbrads...@lcpath.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana
Spears
Sent: Wednesday, April 08, 2009 9:24 AM
To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton
Subject: Re: [Histonet] Benchmark XT
I would definitely check your slides - we have found that when we get a
bad lot of charged slides, things start washing off on the XT -
regardless of detection, antibodies, whatever. It was happening to us
on some tissue types and not on others, but a new batch of charged
slides did the trick.
I've never had to dry 60 min in the oven - 20 does it for us when we
aren't having slide issues.
Also, if you are using any Sta-on or any adhesive in your waterbath with
the charged slides it can cause the slides to sort of repel the tissue
and negate the charged effect again certain tissues will
fall off, others stay on
Dana Spears, HTL(ASCP)
Laboratory Manager
Methodist Medical Center
(309) 672-4930 (office)
(309) 255-7214 (cell)
(309) 279-3768 (fax)
dspe...@mmci.org
Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM
Is anyone out there having problems with tissue washing off the slides
using the Ultraview kit from Ventana?
Mainly we are having problems with needle biopsies washing off
(prostate, liver). Fixation and processing is always the same 4-6 hours
fixation, 3 hour biopsy processing run on VIP processor, cut no thicker
than 4 microns, airdried at least 30 minutes, baked at 59-60 for at
least one hour prior to staining. The instrument's calibrations have
been checked and are fine.
We never had this problem with the iView kit using the Nexes, doing
pretreatments offline.
Any help, ideas, info will be appreciated.
Thanks,
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 35401
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