[Histonet] Cleaning Dissection Tools
Hello Fellow Histonetters, I have some very fine small dissection tools (small Vannas scissors, Dumont #5s, etc) which I use for microdissections (under a dissecting scope.) Afterwards they are often stained with blood or have tissue debris stuck to them. Is there a standard protocol or particular type of soap that you use? I want to get the blood/tissue off, but am afraid of marring/bending the fine tips with scrubbing. I usually soak for a little while in warm soapy water and then lightly clean with a Kim-wipe, but is there a better way? I have also heard some people say they never use soap. Some of my tools I use with PFA and others I use solely on tissue (no PFA) if that makes a difference in how you clean. Thanks for any suggestions! ~Ally Langsdorf Research Technologist Ocular Genomics Institute Massachusetts Eye Ear Infirmary Lab: (617)-573-6485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Anti-human nuclear antibody
You may also want to try the anti-NuMA antibody from cell signaling (#3888) - (rabbit polyclonal). I have found it to be an extremely clean antibody, excellent for detecting even one or two human cells lodged in mouse tissue. Good luck! ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye Ear Infirmary Lab: (617)-573-6485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAP Pen With Aqueous Mounting Media
Hello All, I would like to use a PAP pen to separate sections on my slide, but have been using an aqueous (non-xylenes based) mounting media. How do you remove the PAP pen without using xylenes? Is this done? Thank you, ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye Ear Infirmary Lab: (617)-573-6485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EZ-TMA Kit?
Hi, Has anyone used the EZ-TMA Kit for making your own tissue micro arrays? I am trying to decide on which system to buy. So far, Quick-Ray and Arraymold seem to be the favorites, but I would like to hear if anyone has tried EZ-TMA before I make a decision. Thanks! ~Ally Langsdorf Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
re: [Histonet] ArrayMold System
Hi, I am also interested in hearing about the ArrayMold vs Beecher vs any other do-it-yourself TMA systems. Any favorites systems/recommendations or suggestions? Thanks ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute re: Wondering if anyone has used/purchased the ArrayMold tissue micro array system?? Positive and negative feedback would be greatly appreciated :) Nancy Heath, HT(ASCP) March 10, 2011 is Histotechnology Professionals Day Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Preferred green counterstain for GMS?
Hi, What is your preferred green counterstain for GMS? In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or Tartrazine. Which should I buy? Any help is appreciated! Thanks! ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
