[Histonet] GSH Virtual Summit- THIS SATURDAY

[Lauren Sweeney via Histonet]

Hi Everyone,

It is not too late to join us for the GSH Virtual Summit on May 22, 2021 
beginning at 8:00 am until 4:30 pm.
This virtual meeting will be a great opportunity to get 6 CEU’s through some 
excellent speakers/sessions and we hope to do another in the fall in order to 
stay committed to providing the needed education of histotechs.
Please visit our website at https://georgiahistotech.org/gsh-spring-symposium/ 
for the registration form. Cost is $10.00 per session or $50.00 for all 6 
sessions. You can do 1, 2 or more sessions for the day.
Hope to see you there,
Ely Klar
GSH President


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[Histonet] Fw: GSH Virtual Symposium May 22!

[Lauren Sweeney via Histonet]

Hi All,

Here is the clickable link to register:
https://georgiahistotech.org/gsh-spring-symposium/




From: Lauren Sweeney
Sent: Wednesday, May 5, 2021 2:16 PM
To: Histonet@lists.utsouthwestern.edu 
Subject: GSH Virtual Symposium May 22!


Mark Your Calendars and Join Us!!

The Georgia Society for Histotechnology invites everyone to our Virtual Summit 
on May 22, 2021 beginning at 8:00 am until 4:30 pm.

This virtual meeting will be a great opportunity to get 6 CEU’s through some 
excellent speakers/sessions and we hope to do another in fall in order to stay 
committed to providing the needed education of histotechs.

Please see the attached program with the topics of the sessions and visit our 
website athttps://georgiahistotech.org/gsh-spring-symposium/ for the 
registration form.

Hope to see you there,

Ely Klar

GSH President



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[Histonet] GSH Virtual Summit May 22

[Lauren Sweeney via Histonet]

Mark Your Calendars and Join Us!!

The Georgia Society for Histotechnology invites everyone to our Virtual Summit 
on May 22, 2021 beginning at 8:00 am until 4:30 pm.

This virtual meeting will be a great opportunity to get 6 CEU’s through some 
excellent speakers/sessions and we hope to do another in fall in order to stay 
committed to providing the needed education of histotechs.

Please see the attached program with the topics of the sessions and visit our 
website athttps://georgiahistotech.org/gsh-spring-symposium/ for the 
registration form.

Hope to see you there,

Ely Klar


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[Histonet] job opening

[Lauren Sweeney via Histonet]

Hello Histonet,

There is a job opening for a lab manager position at the University of Georgia. 
 This is a small research lab in the College of Veterinary Medicine. There 
would be 2 techs, including yourself, and 2 pathologists. Since this is a state 
government job, the salary does not compete with private industry. However, 
there are many benefits to working at a state government post, including TRS 
Teachers Retirement (pension- based retirement) and free tuition at any Georgia 
State college, including UGA. If interested, apply here:

https://www.ugajobsearch.com/postings/59337

Best,
Lauren
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[Histonet] re-processing help

[Lauren Sweeney via Histonet]

Hello Histonet,

We have issues- please advise! Long story short- some tissues did not get 
processed over the weekend, they went through the process but were not 
submerged in ANY (we think) of the reagents. Now they are shriveled up and 
brittle (they are intestinal). Is there any way to save them? Can we re-process 
them? Has anyone ever had this problem and had success recovering?

Gratitude,
Lauren
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[Histonet] lab manager position open UGA

[Lauren Sweeney via Histonet]

Good Afternoon,



I reached out a couple of months ago about the Histology Laboratory Manager 
position coming open at the University of Georgia. After a small delay with a 
system change-over, the position is posted! If you are still interested please 
follow the link below to officially apply. Good luck!



https://www.ugajobsearch.com/postings/59337


Lauren





Lauren Sweeney

Laboratory Manager Histology

University of Georgia | College of Veterinary Medicine

Department of Population Health

Poultry Diagnostic Research Center

953 College Station Rd | Athens, GA 30605

706-583-0636 |  lmari...@uga.edu

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[Histonet] link correction

[Lauren Sweeney via Histonet]

Apologies Histonetters,


The working link is: https://www.ugajobsearch.com/postings/59337


for the UGA lab manager position.



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[Histonet] job opportunity

[Lauren Sweeney via Histonet]

Hello Histonetters!

A state- run Histology lab is looking to hire a lab manager. This lab is at the 
University of Georgia in Athens, GA. It is M-F 8 a.m.- 5 pm. shift. Great 
benefits, including University- wide week off at Christmas- paid!

Preferably HT or HTL- certified, IHC experience, and experience as a lab 
manager. Not having these is not necessarily a deal-breaker!

Email me at: lmari...@uga.edu for more information 
and/or to send your resume.

Happy day,

Lauren

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[Histonet] microtome reviews

[Lauren Sweeney via Histonet]

Hi Everyone! We are looking into getting an embedding center and I was 
wondering if I could get some reviews from you about them. Specifically we are 
looking at the Tanner Scientific TN1600, the Avantik EM8, and the Leica 
Arcadia. Any thoughts on any of these would be much appreciated!


Have a wonderful day,
Lauren
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[Histonet] xylene recycling question

[Lauren Sweeney via Histonet]

Hi all you fellow Histonetters!


My query of the day to you is: can you recycle xylene that has been 
contaminated with formalin? (yes, you heard me correctly, formalin)


Thanks for any insight!


L

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[Histonet] feathers

[Lauren Sweeney via Histonet]

Hello Histoland,


A researcher brought us a group of poultry feather shafts to embed and section 
for routine and IHC staining. Has anyone out there had any experience with this 
that could share some advice on getting some successful sections?


So far we have tried facing the blocks and then applying "Nair for Men" for ten 
minutes to the block surface, followed by soaking the blocks for some time 
before attempting sectioning but still we have not had any luck. The sections 
turn out with large holes and blow apart on the water bath.


Any tips would be appreciated, thanks!


Lauren
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[Histonet] Leica embedding station

[Lauren Sweeney via Histonet]

Hello all in Histoland!

We have a Leica EG 1150 H purchased in 2002 and we are starting to have issues 
with it not heating in the cassette and mold warmers as hot as it used to. The 
settings are all the way up to the max (70) but when I measured the actual 
temps it is in the low 50's. Has anyone else had this issue and if so would you 
mind sharing how you dealt with it?

Thanks,

Lauren



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[Histonet] microarray question

[Lauren Sweeney via Histonet]

Hello Histoworld,

I was wondering if anyone out there has any experience with microarrays? Making 
the blocks and cutting them. We have been having a hard time with all the 
nuances of this process and were wondering if you could share any tips/tricks 
you have learned doing this process. Recently, I have been wondering if there 
is a special kind of embedding medium that may work better. We have tried with 
Paraplast and Paraplast Plus and it seems like regular is better in general. 
But even still, we have cracks that form, and sometimes the block pops off 
while cutting on the microtome and also we never seem to have every punch 
unroll when laying the ribbon out on the waterbath.

Please help!
Thanks,

Lauren


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[Histonet] Von Kossa falls off

[Lauren Sweeney via Histonet]

Hi All,

My bone tissue falls right off at the first rinse- after the silver step. Ive 
tried charged and uncharged slides and been VERY gentle. Any tips?

Thanks!


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[Histonet] looking for diff cell counter

[Lauren Sweeney via Histonet]

Good Morning Histonet colleagues,

My PI wants me to purchase a differential cell counter and I was wondering if 
anyone had any recommendations for a basic manual counter. One that offers the 
ability to count 5 different cell types and dings at every 100 cells. I found 
one through Fisher but it seems pricey. Does anyone know other places to get 
them?

Thanks!

Lauren

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[Histonet] overseas jobs

[Lauren Sweeney via Histonet]

Hello all in Histoland,

I am just curious: at most histology conferences I've attended, there's been 
the general consensus that there is a histotech shortage in the US. Does anyone 
know if there is a shortage anywhere else in the world? I am wondering if being 
a histotech is advantageous if you are looking for a job overseas :).

Hope you all  have a great Thursday!

Lauren



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[Histonet] gloves

[Lauren Sweeney via Histonet]

Hello HistoWorld,

My lab is desperately trying to find where to purchase "Cranberry Aqua Source 
Nitrile Gloves" and having no luck. Going to the website listed on the box of 
gloves, there is no way to purchase the gloves online. It just says 'to order 
contact your local dealer'. I called their 800 number and they said that you 
have to contact a distributer and they recommended Henry Schien. When I 
contacted Henry Schien they were very snobbish on the phone and said that there 
was no way to get a quote from them if we aren't already "members" and account 
holders with Henry Schien. Just thought I would ask fellow histotechs if they 
use these gloves and if so, who their distributer is.

Thanks! :)




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[Histonet] plastic section gram stain

[Lauren Sweeney via Histonet]

Hello histonet world,

My pathologist just gave me a slide with a section on it that is intended for 
electron microscopy. Has if anyone ever done a Brown and Hopps special on a 
section embedded in Epon/Araldite?  She told me that her colleague has had the 
sections stained successfully with T-Blue on a hot plate before. But gram 
stains are a bit more complicated than a T-Blue! I can heat the staining 
components (i.e. crystal violet, safranin o, and iodine) but there are removal 
steps that require use of acetone, formaldehyde, acetic acid, and picric acid, 
and I am not sure how those reagents will work with the plastic. Any help or 
experience to share would be wonderful.

Thanks,
Lauren

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[Histonet] blades

[Lauren Sweeney via Histonet]

Hi Histoland-

Blades for grossing tissues- anyone have recommendations? The vendor that we 
were purchasing just straight razor blades from has stopped manufacturing them. 
We don't use scalpels because of their higher cost and we are only working with 
poultry samples.

Thanks and have a great day!


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[Histonet] processor died overnight

[Lauren Sweeney via Histonet]

Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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[Histonet] solvent recyclers

[Lauren Sweeney via Histonet]

Hi everyone,

We are looking into getting a new solvent recycler to recycle our xylene and 
alcohol, any recommendations?

Thanks!



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[Histonet] review

[Lauren Sweeney via Histonet]

Hi all in histoworld,

Has anyone out there used the Thermo Autostainer 360 system before? If so, what 
are your experiences with it? Good, bad, whatever.

Thanks!

L

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[Histonet] IHC question

[Lauren Sweeney via Histonet]

Hello Histonet world,

Our lab has an old Dako Autostainer for IHC and there is a leak in the y-axis 
arm at the point where the tubing goes through a juncture. I was wondering if 
there is anyone out there that uses this in there lab? If so, where do you get 
spare parts for it? Our PI wants us to try to replace the tubing ourselves to 
see if that fixes the problem before getting service.

Thanks!

Lauren

Lauren Sweeney
PDRC Pathology Laboratory Manager
University of Georgia | College of Veterinary Medicine
Department of Population Health
953 College Station Rd | Athens, GA 30605
706-583-0636 |  lmari...@uga.edu

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[Histonet] Wright's stain

[Lauren Sweeney via Histonet]

Hello all in histoland-

Does anyone out there have a recipe for preparing Wright's stain from powder? 
Our pathologist wants us to do Sheehan's modification Giemsa and we don't have 
any commercially prepared Wrights left but we do have an old bottle of Wright's 
powder.

Happy Holidays!

L


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[Histonet] holidays SOP

[Lauren Sweeney via Histonet]

Hello all in Histo Land,

I was wondering if anyone out there works in a Histo Lab that shuts down for 
the holidays? We are closed the week of Christmas and I am preparing the 
laboratory for sitting unattended for a week. If anyone else is in the same 
boat, do you have any tips for what you do to shut down the lab? We are 
planning on emptying the strainers and keeping the coverslipper nozzle 
submerged in xylene as air-tight as possible. Not sure what or if we should 
bother the processors...

Thanks and Happy Holidays!

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[Histonet] product review

[Lauren Sweeney via Histonet]

Hello histoland,

Has anyone out there used Midsci products before? We are considering getting 
slides from them


Thanks!
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[Histonet] ASCP subtest exam scores

[Lauren Sweeney via Histonet]

Does anyone out there who has taken the HT or HTL know what the subtest scores 
mean, how many points they are out of? It says what your total score is out of 
400 but then the subtest scores are all high numbers that do not add up to 400, 
they add up to more so how do you interpret them?
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[Histonet] bone saw

[Lauren Sweeney via Histonet]

Hello histoworld,

Does anyone out there use a bone saw in their lab? We routinely have research 
cases with hundreds of femur head submissions from avian species. We currently 
use a bone saw made by Buehler from the 70's or 80's and it's a work horse, but 
the blade keeps cracking in the diamond tip from overuse during these surveys 
of hundreds of bones. I was wondering what kind of saws are out there that 
could be used for this purpose and if anyone has any experience with this? I am 
looking for something a little more durable, or if not, at least a little 
cheaper. Each blade costs about $350.

Thanks!


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[Histonet] siphon drum pump

[Lauren Sweeney via Histonet]

Good morning and happy Friday everyone!

I am looking to buy some siphon drum pumps- does anyone out there use them? We 
have three and they are all leaking at the squeeze/bulb area from the xylene 
breaking it down. Does anyone know if they manufacture some that are coated to 
protect against xylene?

Thanks!

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[Histonet] veterinary histology

[Lauren Sweeney via Histonet]

To all you veterinary histotechs out there:

We got a case submission of a whole 44 week old chicken head and the write up 
states "swollen head and dirty ear canals". This is the first time we have seen 
this, even the pathologist is stumped. What part(s) of the head would you 
gross, and how?

Any feedback welcome!

Thanks!
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[Histonet] taking the HTL

[Lauren Sweeney via Histonet]

Hello all,

I was wondering if anyone out there has used the HTL practice tests package you 
can buy from ASCP to prepare for their exam, and if you did was it worth $30?

Thanks!

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[Histonet] "Chore chart"

[Lauren Sweeney via Histonet]

Hello all in histoworld,

I have been tasked with creating a "chore chart" of sorts to set up rotating 
division of duties in the lab. I was wondering if anyone else does this, and if 
they do could you take a picture of your chart and paste it in an email so I 
could see an example of one?

Thanks!

Lauren


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[Histonet] chicken brain

[Lauren Sweeney via Histonet]

Hello Histonet world,

I have been charged with the task of opening a week and a half old chicken head 
to retrieve its brain for Histo. Anyone out there done this before and if so, 
what instruments did you use?

Thanks!

L


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[Histonet] block scrapers

[Lauren Sweeney via Histonet]

Hi all,

My lab is in need of some tools to scrap the paraffin off the edges of the 
blocks after embedding. Does anyone have any recommendations for me?


Thanks!!
L
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[Histonet] bone saw blades

[Lauren Sweeney via Histonet]

Hi all, in our lab we have a Buehler Isomet Low Speed Saw that looks like it is 
from the 80's. Up until about 7 months ago, we have used the bone saw fairly 
infrequently. As far as I know, this bone saw wafering blade has never been 
replaced. We now have a researcher who regularly needs sections of bone cut and 
the blade is getting much more frequent use, like hundreds of bones per 
project. My question to you is, how long do these blades stay sharp? Do they 
ever need sharpening? What is their lifespan? What kind of maintenance do they 
need?

Thanks so much!

Lauren
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[Histonet] Trichrome

[Lauren Sweeney via Histonet]

Hello all,

I was wondering if anyone has any experience with doing a trichrome and leaving 
it to mordant in Bouin's for longer than 24 hours. Does this overstain and ruin 
the stain?

Thanks,
L


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[Histonet] biogenex fast red

[Lauren Sweeney via Histonet]

Good Morning all in Histonet land,


I was wondering if anyone has tried to make a smaller quantity of the fast red 
from Biogenex by breaking the tablet in half and using half the amount of 
diluent from the bottles in the kit? If so, how did you do this with the issues 
of the filter tip, etc.


Thanks!


Lauren

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[Histonet] Sakura's Histo-Tek SL Slide Stainer

[Lauren Sweeney via Histonet]

Hello all in Histo land,


I was wondering if anyone out there has been using, or has used, Sakura's 
Histo-Tek SL Slide Stainer? It is for low- volume labs. We are thinking about 
getting one and getting some reviews on it would be great.


Thanks,

Lauren


Lauren Sweeney

Pathology Laboratory Manager

University of Georgia | College of Veterinary Medicine

Department of Population Health

953 College Station Rd | Athens, GA 30605

706-583-0636 |  lmari...@uga.edu

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