Re: [Histonet] "placenta encapsulation"
Like I said, it just gets funnier and funnier. And I agree with the Samurai Pathologist about the original post in this thread: Eeeeww! Lesley. On 03-28-2012, at 10:40 AM, O'Donnell, Bill wrote: > OK, this is too funny. I swear I got this message concerning my last > post. I wonder what triggered it? Are ca**ibals now a protected > minori*y? > > "This email has violated the R-AC-IAL DIS-CRIMI_NATION. > and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. > Message details: > Server: BCHEXEG > Sender: (deleted) > Recipient:(deleted) > Subject: RE: [Histonet] "placenta encapsulation" > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > O'Donnell, Bill > Sent: Wednesday, March 28, 2012 12:33 PM > To: Bob Richmond; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] "placenta encapsulation" > > Now THAT"S NATURAL CHILDBIRTH! > > In my 30 years of histology, I have had two such requests. > (Interestingly, one was about ten years ago - must have been an early > adopter) > > Risk management has a much bigger headache than I do, I just deliver it > to their department, they get it to the patient. > > Question for histonetters - Is this cannibalism? One of the questions I > was never allowed to ask at an interview "What is your feeling about > cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, > I do not advocate cannibalism, and I am sorry if I offeneded any > cannibals (in the vein of redneck lent thread) - Bill > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob > Richmond > Sent: Wednesday, March 28, 2012 12:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] "placenta encapsulation" > > Just when you thought life couldn't get weirder, it does. > > I've been hearing scattered tales of women eating their own (actually > their babies') placentas for years, but I didn't know about "placenta > encapsulation" until my daughter's college classmate Nancy Redd (she's > at nancyredd.com) bylined a New York Times story > http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent > a/?scp=1&sq=placenta&st=cse > > and then got on ABC news this morning. > http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 > > It seems the pill-'n'-potion culture has come to the rescue, with > services (they seem to be local operations, no mail-order, probably how > they slip under the regulatory radar) that for $200 or so will dry and > grind up your baby's placenta and put it into large gelatin capsules. It > has to be your own baby's placenta, by the way. Here's a graphic > demonstration of how it's done > http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst > ructions_w_Pictures > > And here's a FAQ that answers some, but not all questions a pathology > service might have. > http://birth-wise.org/faq > > They want the placenta refrigerated, or possibly frozen. Formaldehyde is > prohibited. > > Seems to me that this could turn into a major headache for a pathology > service, and that some conferring with the necessary people in advance > would be a good idea. > > An obvious concern would be bacterial overgrowth. > > E! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are > not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and delete > the message completely from your computer system. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the > named addressee(s) and contain confidential information. If you are not an > addressee, or responsible for delivering this email to an addressee, you have > received this email in error and are notified that reading, copying, or > disclosing this email is prohibited. If you received this email in error, > immediately reply to the sender and delete the message completely from your > computer system. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet
Re: [Histonet] Re: "placenta encapsulation"
When I had my first baby, someone from the lab where I had been working until just before then arrived to collect the placenta, having asked my permission beforehand. He seemed pretty pleased about it. Lesley. On 03-28-2012, at 6:40 PM, Caroline Miller wrote: > WellI am 6 months pregnant, and was planning on taking a pot of formalin > and a few blades into the delivery suite to get some of my placenta...I > am in real need of a good CD31 and CD34 control! > > We all give our bodies to this - right ;P > > Caroline > > > Caroline Miller, M.Sc, DIC, AIBMS > Senior Research Technologist > Director, Histology and Light Microscopy Core > Gladstone Institutes > 1650 Owens St > San Francisco > CA 94158 > > Tel: 415 734 2566 > Fax: 415 355 0824 > Cell: 415 218 7297 > > http://www.gladstone.ucsf.edu/gladstone/site/histology/ > cmil...@gladstone.ucsf.edu > > > > > > > > > > > > > On Mar 28, 2012, at 5:33 PM, Caroline Miller wrote: > >> >> >> >> >> Begin forwarded message: >> >>> From: "Bartlett, Jeanine (CDC/OID/NCEZID)" >>> Date: March 28, 2012 10:41:50 PDT >>> To: "O'Donnell, Bill" , Bob Richmond >>> , "histonet@lists.utsouthwestern.edu" >>> >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> I thought it was an early April Fool's Day joke.. >>> >>> >>> -Original Message- >>> From: histonet-boun...@lists.utsouthwestern.edu >>> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, >>> Bill >>> Sent: Wednesday, March 28, 2012 1:41 PM >>> To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> OK, this is too funny. I swear I got this message concerning my last post. >>> I wonder what triggered it? Are ca**ibals now a protected minori*y? >>> >>> "This email has violated the R-AC-IAL DIS-CRIMI_NATION. >>> and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. >>> Message details: >>> Server: BCHEXEG >>> Sender: (deleted) >>> Recipient: rsrichm...@gmail.com;histonet@lists.utsouthwestern.edu; >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> -Original Message- >>> From: histonet-boun...@lists.utsouthwestern.edu >>> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, >>> Bill >>> Sent: Wednesday, March 28, 2012 12:33 PM >>> To: Bob Richmond; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> Now THAT"S NATURAL CHILDBIRTH! >>> >>> In my 30 years of histology, I have had two such requests. >>> (Interestingly, one was about ten years ago - must have been an early >>> adopter) >>> >>> Risk management has a much bigger headache than I do, I just deliver it to >>> their department, they get it to the patient. >>> >>> Question for histonetters - Is this cannibalism? One of the questions I was >>> never allowed to ask at an interview "What is your feeling about >>> cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, I >>> do not advocate cannibalism, and I am sorry if I offeneded any cannibals >>> (in the vein of redneck lent thread) - Bill >>> >>> -Original Message- >>> From: histonet-boun...@lists.utsouthwestern.edu >>> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond >>> Sent: Wednesday, March 28, 2012 12:18 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] "placenta encapsulation" >>> >>> Just when you thought life couldn't get weirder, it does. >>> >>> I've been hearing scattered tales of women eating their own (actually their >>> babies') placentas for years, but I didn't know about "placenta >>> encapsulation" until my daughter's college classmate Nancy Redd (she's at >>> nancyredd.com) bylined a New York Times story >>> http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent >>> a/?scp=1&sq=placenta&st=cse >>> >>> and then got on ABC news this morning. >>> http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 >>> >>> It seems the pill-'n'-potion culture has come to the rescue, with services >>> (they seem to be local operations, no mail-order, probably how they slip >>> under the regulatory radar) that for $200 or so will dry and grind up your >>> baby's placenta and put it into large gelatin capsules. It has to be your >>> own baby's placenta, by the way. Here's a graphic demonstration of how it's >>> done >>> http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst >>> ructions_w_Pictures >>> >>> And here's a FAQ that answers some, but not all questions a pathology >>> service might have. >>> http://birth-wise.org/faq >>> >>> They want the placenta refrigerated, or possibly frozen. Formaldehyde is >>> prohibited. >>> >>> Seems to me that this could turn into a major headache for a pathology >>> service, and that some conferring with the necessary people in advance >>> would be a good idea. >>>
Re: [Histonet] FW: Redneck Lent
Me too! And you're welcome. Lesley. On 03-27-2012, at 11:37 AM, Pam Barker wrote: > Hahahahahaha (Im Jewish!) > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: rel...@earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lesley > Weston > Sent: Tuesday, March 27, 2012 2:26 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] FW: Redneck Lent > > It is indeed a joke, an oldie but a goodie. I first heard it years ago about > the Medieval Portuguese Jews who converted to Christianity as the only > alternative to death at the hands of the Inquisition. The Bishop made a > surprise visit to a "convert's" house during Friday-night-supper, so the > householder sprinkled water on the roast chicken, saying "You're fish, > you're fish, you're fish." > > Lesley. > >> For goodness sakes! It is a joke. First of all it was accidently sent to > HistoNet per Joseph's second email. But most of all can't we just loosen up > a bit and laugh at/with each other? Every religion, race, gender, etc. has > had jokes made about it. Give the guy a break. >> >> Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional >> Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l >> PH 804-765-5050 l FAX 804-765-8852 >> >> >> From: histonet-boun...@lists.utsouthwestern.edu >> [histonet-boun...@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE >> [jfra...@hotmail.com] >> Sent: Monday, March 26, 2012 6:48 PM >> To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman >> Subject: [Histonet] FW: Redneck Lent >> >> Date: Mon, 26 Mar 2012 19:55:27 +0100 >> From: spoeri...@yahoo.com >> Subject: Fw: Fwd: Redneck Lent >> To: karen.green...@hobbylobby.com; stewartdap...@hotmail.com; >> yvette.fette...@basf.com; footch...@yahoo.com; >> frazeeli...@hotmail.com; jfra...@hotmail.com;donna.lu...@gmail.com >> >> >> >> >> Kerri >> >> >> >> - Forwarded Message - >> From: Sharen Pray >> To: Ruth Posey ; LueAnn Root >> ; Marjorie Norris ; "Tom >> Voss, Sr." ; Taber Stewart >> ; MONTIE L WINTERS ; Terry >> Maloney ; kerri spoering >> ; "Kenny & Debbie Hager" >> Sent: Saturday, 24 March 2012, 21:06 >> Subject: Fw: Fwd: Redneck Lent >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Each Friday night after work, Bubba would fire up his outdoor grill >> and cook a venison steak. But, all of >> >> >> >> >> >> Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. >> >> >> The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. >> >> The Priest came to visit Bubba, and suggested that he become a Catholic. >> >> After several classes and much study, Bubba attended Mass...and as the > priest sprinkled holy water over him, he said, "You were born a Baptist, and > raised a Baptist, but now you are a Catholic." >> >> Bubba's neighbors were greatly relieved, until Friday night arrived, and > the wonderful aroma of grilled venison filled the neighborhood. >> >> The Priest was called immediately by the neighbors, and, as he rushed into > Bubba's yard, clutching a rosary and prepared to scold him, he stopped and > watched in amazement. >> >> There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: >> "You wuz born a >> deer, you wuz raised a deer, but now you is a catfish." >> >> >> >> Blessings, love and light, "Live simply, love generously, care deeply, > speak kindly." >> >> >> >> >> >> >> ___ >&
Re: [Histonet] FW: Redneck Lent
You're right about your second paragraph. I tried to reply with the original joke, which is indeed about people of the Mosaic faith (only I used the more usual term) surviving the Inquisition, but I got this reply: > This email has violated the RACIAL DISCRIMINATION. > and Quarantine entire message has been taken on 3/27/2012 2:29:28 PM. > Message details: > Server: BCHEXEG > Sender: les...@shaw.ca; > Recipient: Histonet@lists.utsouthwestern.edu; > Subject: RE: [Histonet] FW: Redneck Lent As a J-person, I ought to be be deeply offended, but the whole thing is just getting funnier and funnier. Lesley. On 03-27-2012, at 8:22 AM, Davide Costanzo wrote: > I, personally, found the joke very funny. I find a lot of distasteful jokes > funny - I actually prefer them over anything clean. That does not take away > the fact that discussing religion, or politics (with humor or in any other > form) has no place in the workplace. Histonet is, in many ways, an > extension of the workplace. I also do not discuss religion or politics with > strangers, and there certainly are more strangers that read this blog than > folks we know. While I was not personally offended by that joke, it is very > conceivable to think that some folks would be offended. > > As I told one replier - had this joke been about Jews it would have been > something folks reacted to harshly. And, for good reason. So we cannot joke > about Jews or Muslims, but Catholics are fine? I respectfully disagree - > ALL religions and posts of humor in reference to a religion on a public > listserv is a terrible idea. > > And, incidentally, this support for those that could be offended is coming > from me - a person that thinks ALL religion is a joke in the first place. > > > > On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M wrote: > >> For goodness sakes! It is a joke. First of all it was accidently sent to >> HistoNet per Joseph's second email. But most of all can't we just loosen >> up a bit and laugh at/with each other? Every religion, race, gender, etc. >> has had jokes made about it. Give the guy a break. >> >> Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical >> Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH >> 804-765-5050 l FAX 804-765-8852 >> >> >> From: histonet-boun...@lists.utsouthwestern.edu [ >> histonet-boun...@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [ >> jfra...@hotmail.com] >> Sent: Monday, March 26, 2012 6:48 PM >> To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman >> Subject: [Histonet] FW: Redneck Lent >> >> Date: Mon, 26 Mar 2012 19:55:27 +0100 >> From: spoeri...@yahoo.com >> Subject: Fw: Fwd: Redneck Lent >> To: karen.green...@hobbylobby.com; stewartdap...@hotmail.com; >> yvette.fette...@basf.com; footch...@yahoo.com; frazeeli...@hotmail.com; >> jfra...@hotmail.com; donna.lu...@gmail.com >> >> >> >> >> Kerri >> >> >> >> - Forwarded Message - >> From: Sharen Pray >> To: Ruth Posey ; LueAnn Root ; >> Marjorie Norris ; "Tom Voss, Sr." < >> tomv...@wildblue.net>; Taber Stewart ; MONTIE L >> WINTERS ; Terry Maloney ; >> kerri spoering ; "Kenny & Debbie Hager" < >> kanddha...@att.net> >> Sent: Saturday, 24 March 2012, 21:06 >> Subject: Fw: Fwd: Redneck Lent >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Each Friday night after work, Bubba would fire up his outdoor grill and >> cook a venison steak. But, all of >> >> >> >> >> >> Bubba's neighbors were Catholic. And since it was Lent, they were >> forbidden from eating meat on Friday. >> >> >> The delicious aroma from the grilled venison steaks was causing such a >> problem for the Catholic faithful that they finally talked to their priest. >> >> The Priest came to visit Bubba, and suggested that he become a Catholic. >> >> After several classes and much study, Bubba attended Mass...and as the >> priest sprinkled holy water over him, he said, "You were born a Baptist, >> and raised a Baptist, but now you are a Catholic." >> >> Bubba's neighbors were greatly relieved, until Friday night arrived, and >> the wonderful aroma of grilled venison filled the neighborhood. >> >> The Priest was called immediately by the neighbors, and, as he rushed into >> Bubba's yard, clutching a rosary and prepared to scold him, he stopped and >> watched in amazement. >> >> There stood Bubba, clutching a small bottle of holy water which he >> carefully sprinkled over the grilling meat and chanted: >> "You wuz born a >> deer, you wuz raised a deer, but now you is a catfish." >> >> >> >> Blessings, love and light, "Live simply, love generously, care deeply, >> speak kindly." >> >> >> >> >> >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ---
RE: [Histonet] FW: Redneck Lent
It is indeed a joke, an oldie but a goodie. I first heard it years ago about the Medieval Portuguese Jews who converted to Christianity as the only alternative to death at the hands of the Inquisition. The Bishop made a surprise visit to a "convert's" house during Friday-night-supper, so the householder sprinkled water on the roast chicken, saying "You're fish, you're fish, you're fish." Lesley. > For goodness sakes! It is a joke. First of all it was accidently sent to > HistoNet per Joseph's second email. But most of all can't we just loosen up > a bit and laugh at/with each other? Every religion, race, gender, etc. has > had jokes made about it. Give the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical > Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH > 804-765-5050 l FAX 804-765-8852 > > > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE > [jfra...@hotmail.com] > Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeri...@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.green...@hobbylobby.com; stewartdap...@hotmail.com; > yvette.fette...@basf.com; footch...@yahoo.com; frazeeli...@hotmail.com; > jfra...@hotmail.com;donna.lu...@gmail.com > > > > > Kerri > > > > - Forwarded Message - > From: Sharen Pray > To: Ruth Posey ; LueAnn Root ; > Marjorie Norris ; "Tom Voss, Sr." > ; Taber Stewart ; MONTIE L > WINTERS ; Terry Maloney ; kerri > spoering ; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill and cook > a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were forbidden > from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the priest > sprinkled holy water over him, he said, "You were born a Baptist, and raised > a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, and the > wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed into > Bubba's yard, clutching a rosary and prepared to scold him, he stopped and > watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he carefully > sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, speak > kindly." > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ? on Gulf oil spill
Oil Red O might work, since it stains lipids. Lesley Weston. On 2010-07-28, at 7:26 AM, jstaruk wrote: > Hi all, > > I have a customer who wants to study the amounts of oil accumulation in the > gills of fish from the Gulf of Mexico. Does anyone have any suggestions as > to what techniques might stain crude oil? > > Thanks > > Jim > > ___ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] help
Have you tried sodium thiosulphite? That will leave white spots
instead of brown, which is an improvement and easier to cover.
Lesley Weston.
On 23-Mar-10, at 8:59 AM, Patsy Ruegg wrote:
After you stop laughing seriously I need some help here, apparently
I got my
fingers in some silver nitrate yesterday and touched my face under
my nose
over my lip and now I have black spots that won't come off. I have
done
this on my hands before but never on my face. So far I have tried
soaking a
cloth in hydrogen peroxide hoping to bleach it with no luck, I even
tried
putting some gold chloride on it to see if I could "tone" it down,
to no
avail. Any ideas? Make up only goes so far and lasts so long.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the
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Accordingly
any dissemination, distribution, copying, or other use of this
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dispose of
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Re: [Histonet] a basic question about immunohistochemistry
Just about all biology research institutions have an animal unit with qualified technicians to look after the animals, rather than someone qualified in other areas; however the technicians are subject to human failings such as going home at night. I've never heard of a one- person research team, and I don't think it would be all that effective. The tissue from the animal that died at the wrong time is not comparable to perfused tissue, but as Tom says, it might still be worth processing. If nothing else, it will show how much difference perfusion makes. Lesley Weston. On 28-Nov-09, at 10:17 AM, Thomas Jasper wrote: Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syi...@ucalgary.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT
People have much the same effect. Lesley Weston. On 22-Oct-09, at 12:35 PM, Haynes, MaryAnne wrote: Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
