[Histonet] Dako Autostainer Plus

[Long, Denise M. via Histonet]

Does anyone have the computer with Dako software on it,  that will run an old 
Dako Autostainer Plus IHC instrument? Our computer died but the instrument is 
still in working order.
Agilent/Dako no longer supports or repairs this instrument or the computer that 
runs it, so they are of no assistance.
Y'all are our only hope!


Denise M. Long, MS, HT, HTL (ASCP) QIHC
University of Connecticut
Department of Pathobiology and Veterinary Science
Connecticut Veterinary Medical Diagnostic Laboratory
Histology Laboratory
61 N. Eagleville Road
Storrs, CT  06269-3089
(860) 424-6715


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[Histonet] Trimming guidelines

[Long, Denise M. via Histonet]

Does anyone have  a Trimming guideline or SOP that you can share with me?

Denise M. Long, MS, HTL (ASCP) QIHC
University of Connecticut
Department of Pathobiology
Connecticut Veterinary Medical Diagnostic Laboratory
Histology Laboratory
Storrs, Connecticut 06269-3089
(860) 486-0851
denise.l...@uconn.edu
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Re: [Histonet] Room temperature optimal for Histology Lab

[Long, Denise M. via Histonet]

Thank you!


-Original Message-
From: jdhanna...@gmail.com  
Sent: Wednesday, May 24, 2023 2:27 PM
To: Long, Denise M. 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Room temperature optimal for Histology Lab

*Message sent from a system outside of UConn.*


Hello Denise,

I do not have an answer to your question, but as a consideration when it comes 
to room temperature, it’s important to keep in mind reagent storage temperature 
ranges if you are a CAP (and possibly CLIA) accredited lab.

If you have a reagent that says it should be stored 20C (68F) to 25C (77F) and 
you set your lab to 67F because (hypothetically) there is scientific literature 
saying stating that’s the optimal temperature in a histology lab, then when CAP 
comes and does an inspection, they could site you a deficiency for improper 
reagent storage.

> On May 24, 2023, at 5:00 AM, Long, Denise M.  wrote:
>
> 
> Good morning,
> I'm looking for any scientific based information about optimal room 
> temperature and humidity ranges for a Histology lab. I understand it all 
> depends on your paraffin, but I need something for my meeting with HVAC.
> Can anyone point me to a source document?
>
>
> Denise M. Long, MS, HTL (ASCP) QIHC
> University of Connecticut
> Department of Pathobiology
> Connecticut Veterinary Medical Diagnostic Laboratory Histology 
> Laboratory Storrs, Connecticut 06269-3089
> (860) 486-0851
>
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[Histonet] Room temperature optimal for Histology Lab

[Long, Denise M. via Histonet]

Good morning,
I'm looking for any scientific based information about optimal room temperature 
and humidity ranges for a Histology lab. I understand it all depends on your 
paraffin, but I need something for my meeting with HVAC.
Can anyone point me to a source document?


Denise M. Long, MS, HTL (ASCP) QIHC
University of Connecticut
Department of Pathobiology
Connecticut Veterinary Medical Diagnostic Laboratory
Histology Laboratory
Storrs, Connecticut 06269-3089
(860) 486-0851
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[Histonet] Veterinary Histology Lab Openning

[Long, Denise M. via Histonet]

Hello,

The Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) at the 
University of Connecticut is dedicated to providing top quality diagnostic 
services to our clients, who are comprised of veterinarians, members of the 
agricultural industry, owners of companion, zoo, and aquatic animals, stewards 
of Connecticut wildlife, and residents of Connecticut, New England, and beyond. 
We strive to work cooperatively with federal and state veterinary agencies to 
enhance disease surveillance and response.

Application Deadline is January 3, 2023.



There is a full-time position open at the Connecticut Veterinary Medical 
Diagnostic Laboratory.

This is an entry-level position perfect for recent Histology school graduates. 
HT or HTL  or eligible, no degree required.

No weekends, no major holidays, a State of Connecticut/University of 
Connecticut based job.



https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fjobs.hr.uconn.edu%2Fen-us%2Fjob%2F497046%2Fhistology-technician-laboratory-assistant-for-the-ct-veterinary-medical-diagnostic-laboratorydata=05%7C01%7C%7C48e87cf7cf634005d27808dad7c41121%7C17f1a87e2a254eaab9df9d439034b080%7C0%7C0%7C638059534359643935%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7Csdata=vx9bH8Hm4hthfvOBFwOrcfMapqiNzuTCrvMb%2FoDJMXQ%3Dreserved=0





Denise M. Long, MS, HTL (ASCP), QIHC

Histotechnologist/Immunohistochemist

University of Connecticut

Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical 
Diagnostic Laboratory

61 N. Eagleville Road, Unit 3089

Storrs, Connecticut 06269-3089

(860) 486-0851


Denise M. Long
Histotechnologist/Immunohistochemist
Connecticut Veterinary Diagnostic Laboratory
Department of Pathobiology
University of Connecticut
31 No. Eagleville Road
Storrs, CT 06269
(860) 486-0851

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[Histonet] Connecticut Veterinary Medical Diagnostic Laboratory Job Opening

[Long, Denise M. via Histonet]

Hello,
The Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) at the 
University of Connecticut is dedicated to providing top quality diagnostic 
services to our clients, who are comprised of veterinarians, members of the 
agricultural industry, owners of companion, zoo, and aquatic animals, stewards 
of Connecticut wildlife, and residents of Connecticut, New England, and beyond. 
We strive to work cooperatively with federal and state veterinary agencies to 
enhance disease surveillance and response.

There is a full-time position open at the Connecticut Veterinary Medical 
Diagnostic Laboratory.
This is an entry-level position perfect for recent Histology school graduates. 
HT or HTL  or eligible, no degree required.
No weekends, no major holidays, a State of Connecticut/University of 
Connecticut based job.
https://jobs.hr.uconn.edu/en-us/job/497046/histology-technician-laboratory-assistant-for-the-ct-veterinary-medical-diagnostic-laboratory


Denise M. Long, MS, HTL (ASCP), QIHC
Histotechnologist/Immunohistochemist
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Veterinary Job Opening, Connecticit

[Long, Denise M. via Histonet]

Good afternoon,
The Connecticut Veterinary Medical Diagnostic laboratory at the University of 
Connecticut is searching for  full-time Histotechnologist.
Leave the stress, also no weekends, no major holidays.

See links below for posting and more on the CVMDL.
Home | Connecticut Veterinary Medical Diagnostic Laboratory 
(uconn.edu)
UConn 
Careers


Denise M. Long, MS, HTL (ASCP), QIHC
Histotechnologist/Immunohistochemist
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Veterinary Laboratory job opening in Connecticut

[Long, Denise M. via Histonet]

Good afternoon,
The Connecticut Veterinary Medical Diagnostic laboratory at the University of 
Connecticut is searching for full-time Histotechnologist.
See links below for posting and more information on the CVMDL.
Home | Connecticut Veterinary Medical Diagnostic Laboratory 
(uconn.edu)
UConn 
Careers


Denise M. Long, MS, HTL (ASCP), QIHC
Histotechnologist/Immunohistochemist
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851



Denise M. Long, MS, HTL (ASCP), QIHC
Histotechnologist/Immunohistochemist
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Motic EasyScan Pro and Pro 6 slide scanners

[Long, Denise via Histonet]

Anyone out there using either the Motic EasyScan Pro and Pro 6 slide scanners, 
maybe even the FS LIVE scanner version?
I would love to have some feedback on any of these instruments as well as 
experience with the company service.
thanks,
Denise

Denise M. Long, MS, HTL (ASCP) QIHC
University of Connecticut
Department of Pathobiology
Connecticut Veterinary Medical Diagnostic Laboratory
Histology Laboratory
Storrs, Connecticut 06269-3089
(860) 486-0851
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[Histonet] Canine lymphatics antibody

[Long, Denise via Histonet]

Does anyone in the veterinary or research sector know of an antibody that will 
label canine lymphatics?
I would like information if you have any.
Thanks in advance,
Denise

Denise M. Long, MS, HTL (ASCP), QIHC
Histotechnologist/Immunohistochemist
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Cassette Printers: Leica vs Sakura vs Thermo

[Long, Denise via Histonet]

Hi all,
We're looking at purchasing a new cassette printer. Looking at Leica, Sakura 
and Thermo models.
I'd love to hear good and bad feedback on any model you might be using.
You can send your comments privately.
Thanks in advance,
Denise M Long

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851


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[Histonet] Region 1 Histology Meeting Reminder

[Long, Denise via Histonet]

The Region 1 Histology Meeting sponsored by the Connecticut Society for 
Histotechnologists, Inc. is being held April 15th and 16th at the Mystic Hilton 
in Mystic, Connecticut. We have three lecture rooms going both days for a total 
of 24 speakers, plus a full vendor hall.

Local attractions include the Submarine Force Museum, the USS Nautilus, Mystic 
Seaport and Mystic Aquarium and Institute for Exploration is right across the 
street from the hotel.  Gaming and entertain options are available at nearby 
Foxwoods Resort and Casino, MGM Grand and Mohegan Sun.

For more information on the meeting, including a program, contact Denise at 
denise.l...@uconn.edu
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[Histonet] Microtome calibration

[Long, Denise]

Hi Everyone,
During a recent inspection we were asked by the inspection team to start 
calibrating our microtomes.  Anyone know of a service vendor that will do this? 
Referrals, including self-referrals would be greatly appreciated.
Thank,

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Control block validation requirements

[Long, Denise]

Same inspectors advised our special stain and IHC controls should have at least 
two if not three different validation means (PCR, micro, serology, etc.)
Anyone out there actually doing this?
Just so you know, I've spoken with a few suppliers on control slides and they 
do not do this type of validation on their control material (surprise, 
surprise!)
Thanks,

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Glucose Oxidase blocking method

[Long, Denise]

Hello,
I'm looking for updated information on the Glucose Oxidase blocking method. In 
particular, what reagents are being used now that Sigma's G5250 is no longer 
available.  Does anyone know of a company that has this as a kit?
Thanks,
Denise

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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[Histonet] Prox-1 antibody

[Long, Denise]

Is anyone out there using Prox-1 antibody? In particular, clone 5G10.
Please contact off the list-serv.
Thanks,
Denise


Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851


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