[Histonet] RE: Stainer/coverslipper question
Brett, We just received our new Symphony from Ventana and we love it!! Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, December 03, 2013 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stainer/coverslipper question Hello all, We are looking at options for automated stainer/glass coverslipper workstations. I browsed the Histonet archives and see many varied love/hate responses from over the years about reliability, need to babysit, bubbles etc. and I am wondering if things have improved as many past inquiries were fairly old. From the archives, it seems that Lieca ST5020/CV5030 and the Sakura (Tissue-Tek) Prisma/Glas g2 are the main players. Our HE workload is highly varied, but we want to also use the workstation for dehydrating and coverslipping our immuno slides that come off our IHC stainer in H2O. I would appreciate your recommendations/experiences. Thanks much, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ideal Workflow Lab Design for Histo/Cyto lab Processes
I would also be interested. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, October 31, 2013 4:48 AM To: Histonet@lists.utsouthwestern.edu Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH Subject: [Histonet] Ideal Workflow Lab Design for Histo/Cyto lab Processes Histonetters, I'm looking for information or references concerning the ideal workflow design for a Histo/Cyto lab services. Need to consider ergonomics, work space, equipment, safety, etc. I know there are info. Just need source and experts to chime in. Journal of Histotechnology Advance article etc. Please reply to my work email as well. Ian Bernard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB Testing
Hello All! Can someone recommend a company that does DAB waste testing? I would like to get our waste tested from our Ventana Ultra. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Primera slide printer
Bea, Ditto what Linda said! Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 16, 2013 1:01 PM To: Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Primera slide printer Bea, You can use need to use the clipped corner slides. The best ones are the Epic Scientific slides available from CWS, Inc or the clipped corner Tanner slides available from Mercedes Medical. I'm sure others carry them also. These are the only two slides I've found that work really well in the printer. I am very happy with my slide printer. Set up was very simple and interfacing with our LIS was very easy. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, April 16, 2013 3:56 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Primera slide printer Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura film coverslipper
Marcellyn, I agree with Rene. I have always worked in Histology Labs that have used the Sakura tape coverslipper and have not had any problems. If the amount of xylene that is dispensed is adequate, you should have no problems with the coverslips peeling off over time. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 6:45 AM To: MARCELLYN A. STONE; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Sakura film coverslipper Personally I prefer film coverslipper and used the one by Sakura. Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. The other disadvantage some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of any glass coverslip. Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. René J. From: MARCELLYN A. STONE mst...@cmhlink.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Wednesday, April 17, 2013 9:37 AM Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Reliable Histology Team Member to embed and cut for you.
Well, if I had ever thought of using your services in the past, after reading this post I would never consider it. You should have quit responding a couple of posts ago. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Tuesday, October 02, 2012 7:49 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reliable Histology Team Member to embed and cut for you. Ok, let me put this issue to bed. It's fine you feel you need to be the voice for those who have all the help they need and will never need the services of a histology professional to help cover staffing shortages or inadequate/ineffective staffing. There are thousands of subscribers to this list and I respect every single one of them. HistoCare is for those who NEED professional assistance on an interim basis. And let's be clear, I'm not just a tech looking for job once in a while. I'm a professional extending my expertise and invaluable abilities to help when the need arises on relatively short notice without compromising patient care. Your messages seem to have a very petty tone and not respectful of the nature of the work we do. It's almost as if you are not even of this profession. I care about all those people who go to doctor to have a million tests done to see what's wrong with them and have to wait days and weeks before they get any kind of news to settle their nerves or some glimmer of hope. I want to minimize that inconvenience as much as I can. I am a real person first, a histology professional second on the other side of this email just like there is a real patient on the other side of that slide. You only care about HistoCare advertising on one of the few forums histology professionals have to exchange thoughts, ideas, and RESOURCES. If the best you can add to histonet is criticism, it doesn't need you or those like you. This is a small community with very high turnover, low job satisfaction, some employed individuals with marginal or inadequate abilities, and little interest in others to want to break into this profession. Heck even the ones that's been his field for a long time express dismay, let alone the newbies who can't get the proper support from their own supervisors! Good grief! Those who rely on histonet for advice and ideas should also be able to search for dependable lab support and hope that there is a good resource for them to call upon. Please do me a favor and respectfully bow out and resist the urge to respond further as I have no interest in debating. I'd rather spend my time being productive. There isn't a policy specifically stating i can't make HistoCare available for those who may search for assistance. Lets not forget Histonet is a courtesy to us all for interests in histology and what it is not, is a vehicle to complain. I was initially open to your suggestions for alternatives and offered you the opportunity to respond with solutions acceptable to 'you' but I see you would rather be a complainer than a helper. Any future responses from you referring to this matter directly or indirectly will be considered harassment and forwarded to the appropriate parties. Sincerely HistoCare www.HistoCare.com On Oct 2, 2012, at 4:19 PM, Jay Lundgren jaylundg...@gmail.com wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that occasionally post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25, 9/26, 10/1) Histonet is not a forum for marketing, pitching, plugging, promulgating or selling. I hope it stays that way. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: air drying special stain slides rather than
We have been using the oven dry method for special stains for about four years now and it works wonderfully. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: Tuesday, September 11, 2012 10:15 AM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrateand clear To:histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain;charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to
RE: [Histonet] RE: air drying special stain slides rather than
We use tape coverslipping. Some techs dip the slides in the xylene on stainer before adding them to the coverslipper and some just use the amount of xylene coming out of the drip on the coverslipper. Either works fine and we have had no problems. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, September 11, 2012 10:23 AM To: E. Wayne Johnson; Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Mayer, Toysha N Subject: RE: [Histonet] RE: air drying special stain slides rather than Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: September-11-12 1:15 PM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana
