RE: [Histonet] Mixing Paraffin Brands
Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a harder paraffin Any thoughts??? Pedro L. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. René J. From: Lucie Guernsey lguern...@ucsd.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this.. Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Which fixative for eyes
Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Human paraffin block
Hi everyone, Where can I purchase a human tonsil paraffin block to use in a GLP laboratory? My QA department needs paperwork that accompanies where the tissue came from. Thanks for the help Pedro Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] modified Davidson's fixative on testes
Hello to all Histo techs and Happy early Histotechnology Professionals Day. I wanted to know if anyone out there is using modified Davidson's fixative to fix rat testes and staining with PAS. We have a pathologists that is looking for certain cells within the testes and thought that this might work. Thanks in advance for any information. Pedro Louro Technologist Histology/Immunohistochemistry Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] B-cell Ab to work on rat tissue
Hello out there in Histoland, I'm looking for a B-cell antibody will stain rat paraffin embedded tissues. Anyone out there that has had any luck with this? Thanks in advance for your input. Pedro Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD4, CD8 on Monkey tissue
HI everyone, I wanted to see if anyone out there has had any luck using any CD4 and CD8 antibodies on FFPE monkey tissue. I have used a couple of Ab's that work great on human tissue but no luck with monkey tissue. Any information will be greatly appreciated. Thanks, Pedro Louro, MBA, QIHC IHC Technologist HLS CRO Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Region II Meeting Registration - June 10-12 - Atlantic City, NJ
Anyone around the Atlantic City area on June 10-12? The State Societies of NJ, PA, DE, MD and VA invite you to attend the Region II meeting. Registration is now open. Please click on the link below to view the flyer in pdf format. http://www.keepandshare.com/doc/1857485/2010regioniimeetingbrochure-pdf- april-14-2010-1-38-pm-572k?da=y See you there, Pedro Louro, MBA, QIHC Vice President and Co-Chair Membership Committee New Jersey Society for Histotechnology (NJSH) Ph. 908-473-4501 * This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NJSH 2009 Fall Meeting and Member Appreciation Day
Reminder NJSH 2009 Fall Meeting and Member Appreciation Day Monday, November 2nd Location: New Jersey Hospital Association Conference Center Address: 760 Alexander Road Princeton, NJ 08543 Phone: 609-275-4000 Meeting Schedule: 8:00-9:00 Registration and Continental Breakfast 9:00-12:15 AM Session 12:15-1:15 LUNCH 1:15-4:30 PM Session __ ROOM 1 AM SESSION: Review of Basic Theories of Histotechnology Linda Foster-Brown, MS, HTL(ASCP) AstraZeneca Pharmaceuticals, Wilmington, Delaware This workshop will serve as a review for working Histologists to help them deal effectively with the daily operations of the histology laboratory. It will also serve as overview for the registry eligible candidates preparing for the HT/HTL registry exam. Topics that will be covered include histological theories on the following: lab safety, fixation, processing, microtomy, and staining, as well as registry eligibility requirements. PM SESSION: Troubleshooting Automated Special Stains Debra Cobb, MBA, HT (ASCP) Manager of RD, Artisan In today's world of automation in the Histology Laboratory, troubleshooting special stains and adjusting the workflow has become more complicated. This workshop will address the common issues such as: Background staining on slides, Debris on Slides, Uneven staining, Stain too dark/too light, Workflow, Turn around Time, and will address the statement This doesn't happen when I stain manually! This speaker is sponsored by DAKO. ROOM 2 AM SESSION: Where Do I Find That Antibody What Do I Do With It Once I Have It? Christine 'Charlie' Dorner, HT(ASCP) Dako Applications Specialist - Trainer In the ever changing world of IHC, there are new antibodies and clones being developed every day by many different sources. This seminar will first cover the various ways of locating and obtaining antibodies and different formats that they are available in. Additional discussion will center on choosing Monoclonal or polyclonal antibodies, picking the right detection kit, and use of additional blocking or enhancements. Participants will then learn what to do with new antibodies once they are in their lab including optimization, troubleshooting, deciding on proper control and testing materials and record keeping. Upon completion of this course, the participants will have ideas to aide in the development and structure of an antibody development program for their facility. This speaker is sponsored by DAKO. PM SESSION: Ethics in Imaging Anne Lewin, BS, HT(ASCP)QIHC Bristol-Myers Squibb, Princeton, NJ A growing concern in the scientific community is the ease in which image manipulation can occur. Something that used to be done in a darkroom is now processed digitally, and there are many powerful image editing programs available. Data can be highlighted or erased with ease, and in the case of histology samples, colors can be changed or created in an image. A potential pit-fall is that an image can be altered in an unethical manner without the person realizing it. Many times an image will be cleaned up and important data will be lost or altered. In the case of immunohistochemical analysis, when cell number counts are used for statistical significance, attempting to get a nicer picture could skew the raw data points by missing weakly positive cells or by counting background staining as positive. This workshop will look into the history of image manipulation and the credibility issues journalists have faced since the early days of photography. We will then move onto the many options for digital image capture. Most scientific journals have very few guidelines as to what sorts of digital manipulations are acceptable when preparing a manuscript for submission. Examples of image adjustments will be presented, and potential digital imaging guidelines will be discussed. ___ DIRECTIONS: An extensive list of directions can be found on the NJHA Conference Center Website: www.njha.com/conferencecntr/html/mc.directions.aspx file:///\\www.njha.com\conferencecntr\html\mc.directions.aspx Please park in the rear of the building! This meeting is brought to you in part by: DAKO North America, Inc. NJSH 2009 Fall Meeting Registration Form Mail in Registration Deadline: October 20th Please register early as classroom space is limited! Walk in registrations will be accepted if space is available for an additional $20 fee. __ NAME: __ ADDRESS: ___
