Re: [Histonet] Bodian
Renecould I please get a copy of that procedure ? I would greatly appreciate it. On 10/4/2013 9:35 AM, Rene J Buesa wrote: The fundamental problem with the Bodian stain is the silver protargol. Once you open your expensive bottle (only very few manufacturers of the powder) it starts to oxidize end in only a few days it is no longer able to react properly. That is why I developed a procedure where you prepare your own silver protargol FRESH before every procedure. This, although sound difficult, it is not. Under separate cover I am sending you a publication I did on this method and I am sure you will overcome all your difficulties. René J. From: Helene Degan deg...@upstate.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 3, 2013 4:38 PM Subject: [Histonet] Bodian Hi Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. Thanks again Helene Upstate Medical University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Farewell
Wishing you all the best in your retirement, Sally !! Do try to keep in touch though~~your advice and expertise will be missed !!! Enjoy! LuAnn On 3/30/2012 8:41 AM, Rene J Buesa wrote: Sarah: It has been always a pleasure communicating with you as we have done so many times in the past. You are a real specialist in the difficult field of veterinary histology and you should remember that when in retirement. Try to pass on your knowledge. Being retired does not mean to go into mental hibernation. Participate in HistoNet even more than before. Now you are the owner of your time. My sincere congratulations and enjoy your retirement as I have been doing for the last 10 years. René J. --- On Fri, 3/30/12, Breeden, Sarasbree...@nmda.nmsu.edu wrote: From: Breeden, Sarasbree...@nmda.nmsu.edu Subject: [Histonet] Farewell To: histonet@lists.utsouthwestern.edu Date: Friday, March 30, 2012, 9:16 AM Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re[2]:
I agree~~they need to be deleted from Histonet !! Getting them several times daily ! On 3/27/2012 12:05 PM, Kara Lee wrote: I am getting these constantly as well. Very annoying. Date: Tue, 27 Mar 2012 11:58:39 -0500 From: jmitch...@uwhealth.org To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re[2]: How about these postings to histonet? Is everyone else getting these from Tuyen Nguyen Diamond Ranch Music? If so - can these people/email addresses be deleted from the histonet service? A little redneck humor is one thing - but the love boutique sex shop is another. Jean Mitchell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen Sent: Tuesday, March 27, 2012 11:23 AM To: histonet@lists.utsouthwestern.edu; boost...@diamondranchmusic.org Subject: [Histonet] Re[2]: Welcome, friend! http://loveboutiquesexshop.com/contact.php?yfupu=65ugazylycy=167myroli ma=28 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS staining
I still make my own Schiff's reagent. It is easy to make and I've never had a problem with it. Keeps well in refrigerator. I do make the periodic acid fresh each time. If anyone wants the recipe, let me know. LuAnn On 10/6/2010 11:43 AM, Janet Keeping wrote: I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histology stories
Another funny story: When my son was in kindergarten, the teacher went around the class asking the kids what their parents did. When she got to Michael he stood up and announced quite boldly she cuts up brains!!. The teacher asked me at the next conference what I really did and after explaining histology to her, she told me the story and said that she was afraid to ask him for more details on that day! I got a chuckle out of that and so did she! LuAnn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Victoria Blue stain
VICTORIA BLUE - NUCLEAR FAST RED FOR HBSAg PRINCIPLE To demonstrate copper associated protein, elastic and Hepatitis B surface antigen (HBSAg) in paraffin wax sections. REFERENCE . AFIP laboratory methods in Histotechnology p210 Tanaka method Ref:Acta Pathol Jpn 1981;31:93 SPECIMEN 10% neutral buffered formalin. Standard paraffin wax sections. CONTROL Known case of primary biliary cirrhosis. REAGENTS (A) VICTORIA BLUE Distilled water 200 mL Dextrine 0.5 g Victoria Blue 2 g Resorcinol 4 g Gradually warm up the mixed solution of all the above until it boils. Remove form heat then gradually add 25mL of boiling 29% ferric chloride solution (12mL commercial 60% ferric chloride solution plus 13mL distilled water) and boil the solution for a further 3 minutes. Cool. After cooling filter it. Dry the filtrate on the filter paper in a 56øC oven until completely dry. Dissolve the dried filtrate in 400mL of 70% alcohol. Finally add 4mL concentrated hydrochloric acid and 6g phenol. It is best to leave this solution to mature for at least two weeks prior to use. (B) NUCLEAR FAST RED Dissolve 0.5g nuclear fast red in 500mL of warmed 5% aluminium sulphate. Filter when cool. (C) 4% AQUEOUS SODIUM METABISULPHITE PROCEDURE 1. Deparaffinize and hydrate to distilled water. 2. Treat with acidified potassium permanganate (as for Gordon and Sweet's Reticular fibres method 2.4) for 5 minutes. 3. Treat with 4% sodium bisulphite for 1 minute. 4. Wash in running tap water. 5. Wash well with 70% alcohol. 6. Stain in the Victoria Blue solution, in a Coplin jar, for a minimum of 4 hours (preferably overnight). 7. Wash well with 70% alcohol for approx 1 minute. This is the differentiation step - ensure the background of the section is clear. 8. Wash in running tap water for 1 minute. 9. Stain with nuclear fast red solution for 5 minutes. 10. Wash in running water for 2 minutes. 11. Dehydrate, clear and mount. RESULTS HBsAg, elastic fibres, copper associated protein blue All other tissue components pink/red HEALTH AND SAFETY Potassium permanganate Harmful by ingestion. Irritating to eyes and skin Ferric chloride Irritant. Hydrochloric acid Vesicant. Causes burns. Phenol Harmful by ingestion. Irritating to eyes and skin. At 12:30 PM 11/4/2009, Justin Peters wrote: One of my pathologists has requested that we start offering a Victoria Blue stain for liver specimens. I am unfamiliar with this stain and was wondering if anyone had a protocol for this that works well? Thanks in advance for any help :-) Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone:(804) 967-9225 ext. 1831 Cell:(804) 822-6084 Email: jpet...@bostwicklaboratories.com mailto:jpet...@bostwicklaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] misleading message on Histonet last Friday
I received the message on Friday At 02:35 PM 7/13/2009, Mike Pence wrote: I did not receive this message and have not seen this message come up in the last several weeks. Are you sure this came on the listserv? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership. Dear Linda: Matthew Semovoski [histosea...@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosea...@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair do not try to do any slick sales adds on histonet wanted to make you aware of this. (I really am such a geek LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do just wanted to make you aware of this instance. Thank you so much have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
