Re: [Histonet] Image uploading
Hi Carl, I have sent you a username and password to be able to edit your image posts. Let me know if you have any problems or suggestions. You have definitely posted some great looking images. Best Regards, Marvin Hanna On 07/12/2014 03:17 PM, Hobbs, Carl wrote: I make mistakes when I upload images So, I'd be most grateful for an edit function to be included. Thanks for the opportunity to share images. Sure, the excellent Immunoportal was The Best...I hope that Histonet can equal that website quality? Carlos Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help for identifying the blue stained structure
Hi Rui, You uploaded a tif image and a number of browsers don't support tif images. Jpeg, gif and png images are the best image formats to use because they are universally supported. I converted your image to a jpeg image and posted it at: http://histosearch.com/imageupload/help-for-identifying-the-blue-stained-structure-jpeg/ Best Regards, Marvin Hanna On 05/17/2014 06:39 PM, Rui TAHARA wrote: Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don't think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at?
Hi Patrick, You can upload images for Histonet at http://histosearch.com/imageupload/. The Histonet listserver does not allow attachments to messages to prevent the spread of malware. Let me know if you have any problems. Best Regards, Marvin Hanna On 04/30/2014 01:43 PM, Lewis, Patrick wrote: Hi everyone, What is a good way to post pictures of slides for you guys to look at? I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? Thanks Patrick. PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I just posted 2 photos to histosearch
Hi Patrick, To post more than one image, there is a link under choose file where you uploaded the first picture for add another image. When you click add another image, it opens up another choose file box for a second image to upload. Or you can just post them one at a time if you like. I've seen that black precipitate on slides in an IHC lab I was at a couple of years ago. If I remember correctly, we thought it was a formalin-heme pigment which can be caused when formalin is acidic (not buffered). Marvin On 04/30/2014 02:05 PM, Lewis, Patrick wrote: Thanks everyone, I just posted 2 images to histo search. IS there a way to post up to 5 pics at once? It looked like it suggested that you could post up to 5 pics as a group but I didn't see HOW to do it. Anyway, I have one post of outside of the tissue area, and one post of the tissue area. What is this weird black precipitate? I was using Red AEC as a substrate. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Posting Images for Histonet
Hi Histonetters, Tim Morken asked me about posting images for discussion on Histonet a couple of weeks ago and we noticed our images website is no longer running. As we move into digital pathology, having the ability to post images to discuss on Histonet will become more important. So, we set up an easy way to post images for Histonet on Histosearch at http://histosearch.com/imageupload/. You need to fill in your name, a title for your images, select images to upload from your computer or smartphone and click submit. There is no need to register and it takes about 2 minutes. We intentionally did not include comments on the website so the discussions will take place on Histonet. We have also set up mobile pages for searching Histosearch and the archives at http://m.histosearch.com and http://m.histosearch.com/histonet.html for histologists on the go. You may also want to try out the new image searching capabilities on Histosearch http://www.histosearch.com. After searching with keyword(s) such as ki67 or alcian blue, there is a link at the top of the results for images. Clicking this will provide images related to your keyword(s) from the over 12 million histopathology related pages searched in Histosearch. We believe we have the largest list of histology educational resources on the internet at Histosearch and they are now searchable for text and images. Let us know if we've missed any histology resources. Best Regards, Marvin Hanna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help
Hi Ly, When you click on the Unsubscribe button, an email is sent to you to confirm you want to unsubscribe. It has a link to click to confirm you want to unsubscribe. Is the confirmation email getting lost in your junk folder? You won't be unsubscribed until you click the link in the confirmation email. The confirmation email should have a subject like, confirm 1a5b and a return address of histonet-requ...@list.utsouthwestern.edu. Hope this helps. Best Regards, Marvin Hanna On 12/28/2012 2:04 PM, Ly Nguyen wrote: I want to be remove from the list. I've tried the unsubscribe link be low several time but it seem like it doesn't work because I'm still receiving daily email ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help
Hi Jeanine, Hmmm... The only way that should happen is if someone put your email address in and clicked unsubscribe 3 times. That's the purpose of the confirmation email so that no one else can unsubscribe (or subscribe) you. So, anyone out there trying to unsubscribe Jeanine, please stop. The computer server is smarter than you. And we like Jeanine. :-) Marvin On 12/28/2012 2:33 PM, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: For some reason I just received 3 emails from Histonet asking me to confirm if I want to be unsubscribedI did not request to be unsubscribed. Did anyone else receive emails like this? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marvin Hanna Sent: Friday, December 28, 2012 2:28 PM To: histonet@lists.utsouthwestern.edu; ln0...@gmail.com Subject: Re: [Histonet] Help Hi Ly, When you click on the Unsubscribe button, an email is sent to you to confirm you want to unsubscribe. It has a link to click to confirm you want to unsubscribe. Is the confirmation email getting lost in your junk folder? You won't be unsubscribed until you click the link in the confirmation email. The confirmation email should have a subject like, confirm 1a5b and a return address of histonet-requ...@list.utsouthwestern.edu. Hope this helps. Best Regards, Marvin Hanna On 12/28/2012 2:04 PM, Ly Nguyen wrote: I want to be remove from the list. I've tried the unsubscribe link be low several time but it seem like it doesn't work because I'm still receiving daily email ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Number of blocks
Hi Rene and Histonetters, Thanks Rene. I posted the articles on Histosearch. http://www.histosearch.com/ADP9ProductivityStandards.pdf http://www.histosearch.com/ADP10StaffingBenchmarks.pdf Marvin On 10/25/2012 11:29 AM, Rene J Buesa wrote: Hi Marvin: See attachments. Do you mean that you can post them? I have some other articles about histology topics. Can I send them to you as well? René J. *From:* Marvin Hanna mha...@histosearch.com *To:* Rene J Buesa rjbu...@yahoo.com *Sent:* Thursday, October 25, 2012 11:18 AM *Subject:* Re: [Histonet] Number of blocks Hi Rene, Send the attachments to me and I can post them on Histosearch, Marvin Hannamailto:webmas...@histosearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Reliable... Hi Bill (William DeSalvo), Let's try this: A Newbie's Guide to Histonet
Hi Histocare and any other relatively new people to Histonet, First, hello from Bill and I in beautiful Vancouver and NSH where many histologists are enjoying old friends and making new friends from around the world. Let's remember histology is still a pretty small field in the US with about 25,000 histologists working in about 7,000 labs, plus more and more working in research labs and companies. Over a career, you're likely to meet many of them if you come to enough NSHs. For those of us who have been on Histonet since the beginning (1996?), we would like to remind others of the facts of Histonet: The Histonet listserver is an email listserver for the histology profession that is managed by Dr. Margraf and Dr. Cope-Yokoyama and is run on computers at the University of Texas Southwestern Medical Center. University policies prohibit advertising, but do allow posting of jobs, probably so everybody can dream about being a histologist in some distant place. There are even some temp positions and jobs wanted emails posted every now and then. Histocare, your first posting was no problem and we enjoy seeing all the ways histologists use to market their talents. Three posts in one week is a little redundant. We got it the first time. Companies (Vendors) are permitted to post in response to problems of labs when they have something positive to contribute. Histonet currently has more than 4000 members from throughout the world, with many thousands more who keep up with it through the archives. The archives have over 30,000 visits a month from over 50 different countries. We all want to read questions and answers about problems in histology. Many of us remember before Histonet when labs had to actually solve their problems by themselves. Now over 30,000 times a month a histology problem is solved by one of the eloquent answers of contributors to Histonet. And Histocare, you can be anonymous on Histonet if you like, but you might want to search the archives for others opinions on it. It has been discussed previously. And when you have a website, you can't be anonymous, because I was able to do a whois search and get your name and address. I would recommend using your name and credentials proudly. So, let's get back to solving histology problems on Histonet and leave Dr. Margraf and Dr. Cope-Yokoyama alone. They have patients and stuff they're working on. Just remember to treat others in your profession with respect on Histonet. You just might meet them one day at NSH. And remember to think twice (or three times) before hitting the send button with a negative message. Thousands of us really don't want to hear it. Histonet welcomes all histology questions and a vast majority of the participants think if you don't know the answer, it's not a dumb question, so feel free to ask. Those that don't think so will flame you mercilessly off list for posting, but I recommend you ignore them. A little research in the archives shows me that 94% of the time an email war breaks out on Histonet, testosterone is involved... Respectfully, Marvin Hanna webmas...@histosearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Charged slides
Hi Helen, Plus is the branding for silanized slides, so they are the same. As far as magical, I'm not sure, but the silanization procedure covalently bonds the amino groups in APES (3- Aminopropyltriethoxysilane, available from Sigma) to the silicate ions on the surface of the glass, creating a positive charge on the slide. Polylysine coating also puts a positive charge on the slide, but it is just a coating, unlike the chemically reacted covalent bond of the silanization procedure. A positive charge on the surface of the slide allows for strong bonding with negatively charged formalin fixed tissue. John Kiernan wrote a paper many years back on adhesives for slides that shows the procedure and chemical reaction in silanizing slides. It is available here. http://publish.uwo.ca/~jkiernan/adhesivs.htm Kind Regards, Marvin Hanna On Jan 6, 2011, at 5:43 PM, Helen Fedor wrote: Hi, A question has come up regarding the different methods used to put a charge on the slide. We recently ordered some plus slides and the boxes they are packed in say silanized slides, but they say plus on the slide . We don't want to use these for our clients if they are not going to be getting the same results as the former plus slides that we were using. I was under the delusion that Plus slides somehow are magically charged without any coating process taking place. So does anyone know exactly how a plus slide gets its charge? Do they all get dipped in APES, or Polylysine? Thanks for your help. -- Helen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
