Re: [Histonet] HCV antibody
Hello All, Which HCV antibody is everyone using for FFPE, human tissue?? Thanks, Jo Mauger Children's Hospital of Philadelphia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back orders from various vendors
Martha, Yes, as a matter of fact I have been noticing this too! A real pain. Jo Mauger -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Glacial Acetic Acid subs.
Vinegar should work. Jo Mauger -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Armstrong, Karoleigh T Sent: Tuesday, September 30, 2014 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glacial Acetic Acid subs. Does any one know of a substitute for Glacial Acetic Acid? We use it to clean the lines on the VIP and in the Eosin. Our Corp. heads want to eliminate Glacial in concentration form from all its hospitails. Thanks, Karoleigh Armstrong, HT (ASCP) -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
35 long years!! Jo Mauger -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, August 07, 2014 3:31 PM To: Paula Pierce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 52 years, if we are all posting. Lol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Joe Nocito's request -- oh my!
You're not alone- funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, February 05, 2013 2:42 PM To: Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Joe Nocito's request -- oh my! Am I the only one who thinks its funny Joe the Toe did an unsubscribe e-mail and Cheryl Kerry copied an entire digest to reply. Oh histonet pet peeves... haha On Mon, Feb 4, 2013 at 1:05 PM, Cheryl tkngfl...@yahoo.com wrote: Oh no, Joe! Say it ain't so!! Please tell us this is just to take off your military address and you aren't leaving us forever... Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. --- On Mon, 2/4/13, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IDH1 and IDH2 antibodies, MHC-Class I
Martha, They are now sold through HistoBiotec in the USA. Jo Mauger -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, November 21, 2011 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IDH1 and IDH2 antibodies, MHC-Class I Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: thanks
Thanks to all who answered about mouse macrophages!. I will try F4/80. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth [rcaza...@schosp.org] Sent: Wednesday, July 14, 2010 4:00 PM To: Joanne Clark; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject Hello Histonetters, I have just received the microtome aligner tool from TechOne that Newcomer has. I have used it on my three microtomes and let me tell you guys, I LOVE IT!! It is very easy to use, just follow the instructions that come with it. I strongly recommend it (but that's just my opinion). Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, July 14, 2010 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject Hi All, I just want to clarify that the microtome aligner that we are having issues with is the one made by Advanced Innovations and sold by Newcomer Supply not the one made by Tech One (Shane Perkins) which Newcomer also sells. I have had no experience with the device that Shane is referring to. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mouse macrophage marker
Hi everyone, I need help finding a macrophage marker that works on FFPE mouse tissue. It can not stain myeloid cells! Please share if you know a good one. Thanks, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Has anyone used a biotin block in their antibody diluent?
Jennifer, Vector has an avidin-biotin blocking kit that works well by itself or mixed with reagents- very reasonable cost. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell [jcampb...@vdxpathology.com] Sent: Tuesday, July 06, 2010 10:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC kits
The Leica Bond detection kits are better than LSAB. They are barcoded for the Bondmax machine-not sure if they offer packaged another way. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rchar...@state.pa.us] Sent: Friday, June 18, 2010 10:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC kits Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor needed
Hi All, We are in need of a used tissue processor. If anyone has one for sale in the Philadelphia area, please email ros...@email.chop.edu. Budget is very small!!! Thank you. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF [karen.heckf...@chw.edu] Sent: Monday, April 19, 2010 7:57 AM To: Michelle MacVeigh-Aloni Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Does anyone have and use Shandon Excelsior EX I have had one for a few years now and love it. You can also monitor it from another location if you have analog internet. It is really easy to change out the reagents and is way less messy than most other processors. You need to make sure your reagents stay topped off. The only real problems I have had with it is a Pathologist loading it wrong and not setting it down on the tongs correctly and breaking my basket that the cassettes set into. That was kind of a nightmare. I had to retrain the Pathologist. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Friday, April 16, 2010 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone have and use Shandon Excelsior EX Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Thanks- CD3 on mouse
Thanks for your very helpful replies- as always. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: James L Burchette [burch...@mc.duke.edu] Sent: Thursday, March 18, 2010 12:03 PM To: pru...@ihctech.net Cc: histonet@lists.utsouthwestern.edu; Mauger, Joanne; Emily Sours Subject: RE: [Histonet] CD3 on mouse Patsy - I agree. LV's rabbit MoAb works well on mouse tissue. They also have a rabbit polyclonal CD20 that works well on mouse. jb pru...@ihctech.net pru...@ihctech.net Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2010 11:58 AM Please respond to pru...@ihctech.net To Mauger, Joanne mau...@email.chop.edu, Emily Sours talulahg...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] CD3 on mouse Joanne, the rabbit polyclonal or rabbit monoclonal cd3's work well on mouse tissue, dako has poly and lab vision i believe has a rab mono, it may be poly too, the key is that it is rabbit and not mouse. From: Mauger, Joanne mau...@email.chop.edu Sent: Wednesday, March 17, 2010 1:34 PM To: Emily Sours talulahg...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD3 on mouse Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonethttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Where did Dezna C. Sheehan practice?
Hi All, Dezna Sheehan worked at the Hospital of the University of Penn. for many years. She ran a school for Histotechnology which I and many of my friends and colleagues were lucky to have gone to. We are proud to continue her push for the advancement of our field. Happy Histotechnology Professionals Day!! Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Veronica Coker [veronica...@hotmail.com] Sent: Wednesday, March 10, 2010 6:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where did Dezna C. Sheehan practice? Happy Histotechnology Professionals Day! One of my Professors told me that Sheehan(of Theory and Practice of Histotechnology-by Dezna C. Sheehan, Barbara B. Hrapchak) practiced her work in Philadelphia. I have interest in this for I am originally from Philadelphia. Does anyone know where she did most of her work? Thank You, -Veronica Coker _ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: BK Virus Antibody
Hi Jim, We use the SV40(large T-antigen) antibody from BDBioscience #554154. Vector has a different antibody for polyomavirusJC/BK viruses, #VP-P973. Both work well for us in FFPE. The problem we always have is no positive control tissue! Good luck. Jo Joanne Mauger From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim [vickroy@mhsil.com] Sent: Tuesday, December 22, 2009 2:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BK Virus Antibody We used to purchase our BK virus antibody from a company called Chemicon. Apparently they were bought out by Millipore and the antibody is not available from them. I need to get the antibody from a new source. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
