[Histonet] Plastic to Paraffin

[Metzger, Kenneth via Histonet]

Hello All,

Our EM lab melted a block meant for IHC staining and put it into plastic. Is 
there any way to get the tissue back to paraffin?

I know it's a long shot but just wondering

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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[Histonet] Staples and Sutures

[Metzger, Kenneth via Histonet]

Hello All,

Recently we have had a marked increase in staples and sutures in our tissue 
that arrives for processing. I have tried to relay to our pathologists and PA's 
the difficulty in cutting sections and the tissue destruction in getting 
staples and sutures out once the tissue is processed to paraffin. I was told by 
some of them that in their experience histology should dealing with this issue. 
Though my own opinion is this is position is not valid, I've been asked to 
collect opinions from other histology departments if there are protocols that 
the grossing individual should be removing the foreign object. Please give me 
any feedback...Thanks

Ken

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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[Histonet] RE: Cornflaking artifact

[Metzger, Kenneth]

Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing 
this as well out of the blue..haven't changed a thing.

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, 
SHEILA
Sent: Thursday, March 13, 2014 9:42 AM
To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

We also have recently started to see this artifact more than ever before, and 
nothing in our process has changed.  We have tried everything to correct to no 
avail.  Wonder if it is possible to be a change in some type of supply, either 
xylene or coverslipping film.  Something has changed but am at a loss as to 
what.


Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

You will also see the cornflaking if your tissue is lifting off of the slide at 
all.  We used to get this more often on hard, decal specimens than on other 
specimens.  We used the film to coverslip.  If you remove the film from the 
problem slides and recoverslip conventionally with extra mountant and glass 
coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with cornflaking (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper to 
make sure it was functioning properly, including the xylene drip.  We continue 
to have this artifact and it is driving us crazy.  It is sporadic with no 
pattern of tissue type or placement on the slide.  Sometimes it lands on tissue 
other times not.  Most of the time when we remove the coverslip and 
re-coverslip it goes away (I am assuming because the acetone removes any minute 
amounts of water that may be present).  We just cannot figure out where the 
water is coming from.  Has anyone seen this artifact while using the drying 
step on the prisma stainer?  We just recently started using the drying on some 
slides and I am thinking maybe it is causing humidity???  I cannot say for a 
fact that our cornflaking started at the same time, but it is suspicious. 
HELP!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffinwhat is 
the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double tetracycline 
label?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
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[Histonet] Vacuole appearance in lymph Nodes

[Metzger, Kenneth]

Hi All,

I have sent this question to respected colleagues individually so pardon my 
redundancy if you have this email already.

Lately we have had random nodes show up with areas that appear to have multiple 
vacuoles through-out the specimen.  They have different patterns section to 
section so I know it is not in the tissue. The staining looks fine and the 
tissue cuts well so I don't think it is a processing issue. Any ideas on the 
problem would be welcome. Thanks

Ken


Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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[Histonet] Recall: Node Issue

[Metzger, Kenneth]

Metzger, Kenneth would like to recall the message, Node Issue.

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[Histonet] Recall: Node Issue

[Metzger, Kenneth]

Metzger, Kenneth would like to recall the message, Node Issue.

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[Histonet] HT exam

[Metzger, Kenneth]

Can someone tell me what year they began requiring college credits for the HT 
exam? Thanks

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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[Histonet] Control Tissue

[Metzger, Kenneth]

Does anyone have or know where I can get control tissue for the MYCN (2p
24.1) FISH probe assay, an amplified or positive control?  Thanks

 

 

Kenneth G Metzger HTL(ASCP)

Histology Supervisor

ARUP Labs

Salt Lake City, Utah

Phone: (801)583-2787 ext. 3101

Fax: (801) 584-5244

Email: kenneth.metz...@aruplab.com

 


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[Histonet] Whole Mount Coverslips

[Metzger, Kenneth]

Can anyone tell me who sells whole mount coverslips besides Brain
Research Labs? They have mine back-ordered until 10/8/09 and I need some
before then. Thanks,

 

Ken

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101

 


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[Histonet] Tampers

[Metzger, Kenneth]

Hello Everyone,

 

Can anyone give me a vendor and order # for tampers to be used while
embedding? I appreciate any info. 

 

Ken

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101

 


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[Histonet] KP Marker Plus

[Metzger, Kenneth]

Does anyone know who sells the KP Marker PLUS? I can't seem to find
them. Thanks for any help.

 

Ken

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101

 


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[Histonet] Whole Mount Immuno Question (again)

[Metzger, Kenneth]

Can anyone tell me where to buy a slide holder or chamber to perform
manual whole mount immunos? Something like the slide master from Scytek
would be great but I won't get my hopes up!! Thanks,

 

ken

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101

 


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[Histonet] Whole Mount Immunos

[Metzger, Kenneth]

One of my pathologist would like to do PSA, P504S (alpha-methylacyl-CoA
racemase), and CD31 on whole mount prostates. Is there anyone out there
doing this and if so could I ask some questions of you? We have not done
immunos on whole mounts before and since no automation supports whole
mount slides (we have Ventana and Dako) I would have to go back to
manual...which I haven't done in a LONG time. Recommend any kits,
protocols, ect...Any help would be appreciated. Thanks,

 

Ken

 

PS Thanks for the help getting the charged whole mount slides from my
earlier email.

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101

 


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[Histonet] Full Mount Immunos

[Metzger, Kenneth]

Does anyone know of any companies producing charged full mount slides? I
have a pathologist that wants immunos done on full mount prostates and
the tissue keeps falling off. I have looked, but cannot find any charged
full mount slides. Any help would be appreciated!!

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101

 


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[Histonet] Block shippers

[Metzger, Kenneth]

Recently we have had paraffin blocks shipped to our facility in clear,
four chambered carriers. Can anyone tell me where I can get these?

 

Thanks,

 

Ken

 

Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

[EMAIL PROTECTED]

(801) 583-2787 x 3101

 


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