[Histonet] core histolgy recharge rates
Hello all, I am trying to get a feel for how other core histology facilities are charging. My main question is if or when technician's labor time is charged. Currently we charge a fee per sample for embedding, whether it is paraffin, plastic, or oct. Then we charge a fee per slide/grid for cutting, and fee per slide/grid for staining. Any time the technician is doing the work there is also a labor/tech time fee per hour. Any information you are willing to share is very much appreciated. Thanks, Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Paraffin Tissue Crumbles
Hello to everyone, Thank you for all the advice. I will reduce my processing time as well as try to rehyrdate my paraffin blocks before cutting. I greatly appreciate the help. Thanks, Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Tissue Crumbles
Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5°. Sections were 5µm thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] protocol for Masson-Goldner trichrome modified by Ulrich
Hello everyone, I'm trying to use the Masson-Goldner Trichrome stain, and I came across a paper that used one that was modified by Ulrich. There was no citation on the paper, and I wanted more details to their protocol, i.e. how they made their solutions. The paper is titled Histology and research at the hard tissue-implant interface using Technovit 9100 New embedding technique by Elmar Willbold http://www.sciencedirect.com/science?_ob=ArticleURL_udi=B7GHW-50CVR3J-1_u ser=4429_coverDate=06%2F25%2F2010_rdoc=1_fmt=high_orig=search_sort=d_d ocanchor=view=c_searchStrId=1436969926_rerunOrigin=google_acct=C5960 2_version=1_urlVersion=0_userid=4429md5=ac8acdf1a211881097275a05690a4259 #cor1 and Frank Witt. Does anyone know more details on this stain? We are using it on hard tissue embedding, where we used methylmethacrylate. Thanks for the help! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
