RE: [Histonet] Tissue-Tek VIP 2000-3000

2010-08-20 Thread Nancy Klemme

Just to reinforce Joe's comments and clarify information regarding the VIP K 
series processors (VIP 1000, 2000  3000):

- Sakura manufactured these for Miles until 1993.
- The first two digits of the serial number identify the year it was 
manufactured.(82-92)
- Never purchase a unit without a serial number affixed to the unit.
- Sakura no longer services these units.
- Parts are no longer being manufactured for these units.
- However, many parts are still stocked by Sakura and available for purchase.
- Some new or used parts may be available through independent service companies.
- As Joe stated, be responsible with routine maintenance.
- They've been around longer than the (trademarked) Energizer Bunny.
- The fact that many people still use these models is proof of their 
reliability.

Kind Regards,
Nancy Klemme
(Another old work horse)
Education / Training, Technical Services

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Saby
Sent: Friday, August 20, 2010 2:45 PM
To: Fimbres, Amber; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000

Mike-

Although Sakura is no longer making parts for the VIP 2000 units, there are
thousands still in use around the country.  Most companies that service these
units have been taking them in trade as people upgrade their equipment, then
using these units to provide parts for units in trouble along the way.  They are
very dependable (with regular water rinses and periodic PM).

Sakura does still makes parts for and service the VIP E300s, which is the step
up from the 2000.  If the price were right, and funds were low, I would buy
either. I have been using these units for many years.

Joe Saby, BA HT





From: Fimbres, Amber afimb...@uci.edu
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Fri, August 20, 2010 5:17:46 PM
Subject: [Histonet] Tissue-Tek VIP 2000-3000


Mike,

I know I'm a little late regarding your question about Sakura's VIP 2000/3000.
I'm not sure if you found your answer, but if you're talking about the VIP
processor that uses a magnet to program, change stations, etc. (and is reddish
orange in color and may even say 'Miles' on it instead of Sakura) you will want
to think twice before purchasing it.  These VIP K (series 1000, 2000, 3000) are
no longer serviced by Sakura nor are there many parts for them.  Sakura's
manufacturer for parts has stopped making replaceable parts for this particular
model (they are at least 20 or more years old).  You can go to Sakura's website
or call their technical support, they will confirm this too.

Take care,

Amber



  
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[Histonet] RE: Experience with Tissue Tek VIP6 processor

2010-01-15 Thread Nancy Klemme
Hello, Dr. Rabe:

I working on my 40th year as a histologist and remember hearing or reading of 
tissue processing by going from isopropanol directly into the paraffin step 
that had taken place in the 1950's or '60's.  I'm not sending this as a 
declaration of my age, but, rather as a reference that xylene-free processing 
has been around for a long, long time, even though most of us used a clearing 
step between the dehydrant and the paraffin steps.  ANY tissue processor (human 
or mechanical) can be used for this technique because the key elements are the 
chemicals that the tissues are being exposed to.

Because attachments cannot be included, as a private eMail, I will send you a 
White Paper that Sakura published last year using this technique with the VIP6 
that you're considering.  Anyone reading this may obtain it from your Sakura 
Sales Manager or simply send me a return eMail.  I'll also request to have it 
placed on our company web-site.  As with any change in specimen handling 
procedure, you will want to document your validation of this technical change.

Whatever instrument you ultimately choose to purchase, I send my kind regards.
Nancy Klemme
Edu. Svcs. Dir. / Sakura Finetek USA, Inc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe
Sent: Friday, January 15, 2010 8:05 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Experience with Tissue Tek VIP6 processor

Our lab is in the market for a new processor.  Does anyone have experience with 
the Tissue Tek
VIP 6?  And does anyone have experience using it xylene-free?  Our histology 
supervisor would
love to talk with you.

Kim Rabe, M.D.
North Central Pathology, P.A.

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RE: [Histonet] Re: Sakura iDent

2010-01-13 Thread Nancy Klemme
Dear Annie,

I am writing you from California where our Wednesday workday is beginning and 
you are preparing for your Wednesday to draw to a close.

Although the majority of Sakura Finetek products are carried by all Sakura 
Finetek entities, the iDent is not one that is available from Sakura Finetek 
USA in North America.   If most Histonet subscribers are from the U.S., that 
may explain the very little response from the histonet that you noted.

I am sorry about your cassette labeler issues and have forwarded your posting 
to the gentleman who is in charge of Sakura Finetek Middle East.  His name is 
Mr. Ghaleb Alkhaseb and I am sure he will be in contact with you very soon 
after reading it.  I do not know what Sakura contact information you have been 
using, but I know that Mr. Alkhaseb will make sure that you will have the most 
current information for a more direct company contact.  I do expect your iDent 
situation will be corrected and resolved to your satisfaction very soon.

From 12 hours behind you, I offer my kindest regards.

Nancy Klemme, HT(ASCP)
Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA
800-725-8723 x7879

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van 
Binsbergen
Sent: Wednesday, January 13, 2010 1:16 AM
To: Denise Van Eaton
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Sakura iDent

Hi Denise
sadly no resolution
also sadly very little response from the histonet - except for Rene - he and
i did some troubleshooting to try to see if i had missed anything - we had
the same thoughts
so ... i am still sitting with the iDent in its box, unused, until someone,
somewhere can convince me that the print does not wipe off with xylene  -
which will be hard to do seeing that i wiped it off myself and saw with my
own eyes that xylene removes the ink!!

Sakura - if you are reading this - i am not a happy camper

Annie
2010/1/12 Denise Van Eaton dvanea...@littonlab.com

  Hi Anne,

 We have been checking into cassette printers. Obviously, if we can bring
 one in for a demonstration we will but I thought the iDent looked like a
 good place to start. I noticed your problem (on the Histonet) with the ink
 rubbing off after the xylene. I never saw any responses from the rest of the
 Histonetters... did you resolve the problem? Assuming you (or Sakura) did,
 what was the trick?

 Thank you in advance for sharing your fix for a potential problem.

 Denise Van Eaton HT(ASCP)
 Litton Pathology Associates




--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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RE: [Histonet] Silver Nitrate instead of inking??

2009-12-17 Thread Nancy Klemme
Dear Sheila,

I would strongly recommend that you obtain approval from the manufacturer of 
the tissue processors into which you will be putting these tissues before you 
would begin this practice.  The negative accumulative affect on several of the 
internal components of the instrument can (and probably will) eventually cause 
the instrument to fail.

You would see the effect of the silver nitrate on any metal products it would 
come into contact with: forceps, metal holding container for the racks or 
baskets, the racks or baskets themselves if they are metal.

It is not approved for use on any model of Tissue Tek Tissue Processors.

Many years ago this practice was tolerated by the old tissue-transfer 
processors.  Some people even used this inking method and placed tissues on 
earlier models of the fluid-transfer processors.  Service that was required in 
those years did not include investigation as to what was placed into the 
instrument (not simply the reagents, but what also came in on the specimens) 
that could have been the root cause of the problem.

I hope this informative and that you continue to use your current inking 
product or investigate others that are safer for you and your processors.

With kind regards and wonderful holiday wishes,
Nancy Klemme
EduSvcsDir - Sakura Finetek

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila adey
Sent: Thursday, December 17, 2009 3:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silver Nitrate instead of inking??



Hi Everyone,



Has anyone ever heard of using Silver Nitrate for inking skin bx's before 
processing???

One of our paths mentioned it.



Thanks in advance.


Sheila Adey HT MLT



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[Histonet] RE: Tampers

2009-08-20 Thread Nancy Klemme
Hello, Ken:
The following is from the Sakura on-line electronic catalog.  They're available 
through Cardinal Health or VWR.  Be sure to identify the item number as being a 
Sakura product number.

Item No.Description QuantityPrice
1551TAMPER, LARGE   THREE   $19.00
1552TAMPER, SMALL   THREE   $15.00

Kind regards,
Nancy Klemme, EduSvcDir - Sakura Finetek USA, Inc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth
Sent: Thursday, August 20, 2009 8:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tampers

Hello Everyone,



Can anyone give me a vendor and order # for tampers to be used while
embedding? I appreciate any info.



Ken



Kenneth G Metzger HTL(ASCP)

ARUP Labs

Histology Supervisor

500 Chipeta Way

Salt Lake City, Utah 84108-1221

kenneth.metz...@aruplab.com

(801) 583-2787 x 3101




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RE: [Histonet] metal embedding base molds

2009-08-06 Thread Nancy Klemme
Dear Vikki,
Sakura still carries the metal base molds that are used with Embedding 
Rings. They do not have the wings that the ones for use with the Unicassettes 
have.  Their Sakura part numbers begin from 4121 and higher.  If you use the 
electronic catalog on Sakura's website (www.sakuraus.com) and search by product 
description, just type in base molds.  Then click on part # 4121 and the 
wingless ones will be pictured.

Kind regards,
Nancy Klemme, Sakura Educational Services


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Thursday, August 06, 2009 10:13 AM
To: histonet
Subject: [Histonet] metal embedding base molds

Hi-

I'm trying to locate embedding molds that Sakura/Tissue Tek makes.  All I'm
able to find at this point are the metal molds with the 'wings' on them.

Does anyone know a source?  With all the mergers etc it's been a little
challenging.

Thanks in advance

Vikki
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