RE: [Histonet] Tissue-Tek VIP 2000-3000
Just to reinforce Joe's comments and clarify information regarding the VIP K series processors (VIP 1000, 2000 3000): - Sakura manufactured these for Miles until 1993. - The first two digits of the serial number identify the year it was manufactured.(82-92) - Never purchase a unit without a serial number affixed to the unit. - Sakura no longer services these units. - Parts are no longer being manufactured for these units. - However, many parts are still stocked by Sakura and available for purchase. - Some new or used parts may be available through independent service companies. - As Joe stated, be responsible with routine maintenance. - They've been around longer than the (trademarked) Energizer Bunny. - The fact that many people still use these models is proof of their reliability. Kind Regards, Nancy Klemme (Another old work horse) Education / Training, Technical Services -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Friday, August 20, 2010 2:45 PM To: Fimbres, Amber; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000 Mike- Although Sakura is no longer making parts for the VIP 2000 units, there are thousands still in use around the country. Most companies that service these units have been taking them in trade as people upgrade their equipment, then using these units to provide parts for units in trouble along the way. They are very dependable (with regular water rinses and periodic PM). Sakura does still makes parts for and service the VIP E300s, which is the step up from the 2000. If the price were right, and funds were low, I would buy either. I have been using these units for many years. Joe Saby, BA HT From: Fimbres, Amber afimb...@uci.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Fri, August 20, 2010 5:17:46 PM Subject: [Histonet] Tissue-Tek VIP 2000-3000 Mike, I know I'm a little late regarding your question about Sakura's VIP 2000/3000. I'm not sure if you found your answer, but if you're talking about the VIP processor that uses a magnet to program, change stations, etc. (and is reddish orange in color and may even say 'Miles' on it instead of Sakura) you will want to think twice before purchasing it. These VIP K (series 1000, 2000, 3000) are no longer serviced by Sakura nor are there many parts for them. Sakura's manufacturer for parts has stopped making replaceable parts for this particular model (they are at least 20 or more years old). You can go to Sakura's website or call their technical support, they will confirm this too. Take care, Amber This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Experience with Tissue Tek VIP6 processor
Hello, Dr. Rabe: I working on my 40th year as a histologist and remember hearing or reading of tissue processing by going from isopropanol directly into the paraffin step that had taken place in the 1950's or '60's. I'm not sending this as a declaration of my age, but, rather as a reference that xylene-free processing has been around for a long, long time, even though most of us used a clearing step between the dehydrant and the paraffin steps. ANY tissue processor (human or mechanical) can be used for this technique because the key elements are the chemicals that the tissues are being exposed to. Because attachments cannot be included, as a private eMail, I will send you a White Paper that Sakura published last year using this technique with the VIP6 that you're considering. Anyone reading this may obtain it from your Sakura Sales Manager or simply send me a return eMail. I'll also request to have it placed on our company web-site. As with any change in specimen handling procedure, you will want to document your validation of this technical change. Whatever instrument you ultimately choose to purchase, I send my kind regards. Nancy Klemme Edu. Svcs. Dir. / Sakura Finetek USA, Inc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Friday, January 15, 2010 8:05 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Experience with Tissue Tek VIP6 processor Our lab is in the market for a new processor. Does anyone have experience with the Tissue Tek VIP 6? And does anyone have experience using it xylene-free? Our histology supervisor would love to talk with you. Kim Rabe, M.D. North Central Pathology, P.A. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Sakura iDent
Dear Annie, I am writing you from California where our Wednesday workday is beginning and you are preparing for your Wednesday to draw to a close. Although the majority of Sakura Finetek products are carried by all Sakura Finetek entities, the iDent is not one that is available from Sakura Finetek USA in North America. If most Histonet subscribers are from the U.S., that may explain the very little response from the histonet that you noted. I am sorry about your cassette labeler issues and have forwarded your posting to the gentleman who is in charge of Sakura Finetek Middle East. His name is Mr. Ghaleb Alkhaseb and I am sure he will be in contact with you very soon after reading it. I do not know what Sakura contact information you have been using, but I know that Mr. Alkhaseb will make sure that you will have the most current information for a more direct company contact. I do expect your iDent situation will be corrected and resolved to your satisfaction very soon. From 12 hours behind you, I offer my kindest regards. Nancy Klemme, HT(ASCP) Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA 800-725-8723 x7879 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, January 13, 2010 1:16 AM To: Denise Van Eaton Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sakura iDent Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton dvanea...@littonlab.com Hi Anne, We have been checking into cassette printers. Obviously, if we can bring one in for a demonstration we will but I thought the iDent looked like a good place to start. I noticed your problem (on the Histonet) with the ink rubbing off after the xylene. I never saw any responses from the rest of the Histonetters... did you resolve the problem? Assuming you (or Sakura) did, what was the trick? Thank you in advance for sharing your fix for a potential problem. Denise Van Eaton HT(ASCP) Litton Pathology Associates -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silver Nitrate instead of inking??
Dear Sheila, I would strongly recommend that you obtain approval from the manufacturer of the tissue processors into which you will be putting these tissues before you would begin this practice. The negative accumulative affect on several of the internal components of the instrument can (and probably will) eventually cause the instrument to fail. You would see the effect of the silver nitrate on any metal products it would come into contact with: forceps, metal holding container for the racks or baskets, the racks or baskets themselves if they are metal. It is not approved for use on any model of Tissue Tek Tissue Processors. Many years ago this practice was tolerated by the old tissue-transfer processors. Some people even used this inking method and placed tissues on earlier models of the fluid-transfer processors. Service that was required in those years did not include investigation as to what was placed into the instrument (not simply the reagents, but what also came in on the specimens) that could have been the root cause of the problem. I hope this informative and that you continue to use your current inking product or investigate others that are safer for you and your processors. With kind regards and wonderful holiday wishes, Nancy Klemme EduSvcsDir - Sakura Finetek -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Thursday, December 17, 2009 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT _ Eligible CDN College University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tampers
Hello, Ken: The following is from the Sakura on-line electronic catalog. They're available through Cardinal Health or VWR. Be sure to identify the item number as being a Sakura product number. Item No.Description QuantityPrice 1551TAMPER, LARGE THREE $19.00 1552TAMPER, SMALL THREE $15.00 Kind regards, Nancy Klemme, EduSvcDir - Sakura Finetek USA, Inc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Thursday, August 20, 2009 8:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tampers Hello Everyone, Can anyone give me a vendor and order # for tampers to be used while embedding? I appreciate any info. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal embedding base molds
Dear Vikki, Sakura still carries the metal base molds that are used with Embedding Rings. They do not have the wings that the ones for use with the Unicassettes have. Their Sakura part numbers begin from 4121 and higher. If you use the electronic catalog on Sakura's website (www.sakuraus.com) and search by product description, just type in base molds. Then click on part # 4121 and the wingless ones will be pictured. Kind regards, Nancy Klemme, Sakura Educational Services -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet