[Histonet] Movat's Pentachrome in ChicagoLand?
If anyone is running Movat's Pentachrome stain in the Chicago Area, could you please contact me? Thanks Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Pr on Ventana?
Hi Friends, If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you please contact me separately? I'd like some advice. Thank you Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 -Original Message- Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Melanoma sentinel nodes
Amy, I can't find the exact reference but I think either Balch or Taylor authored the paper that was adapted for our protocol. Level 1, 3, 5, 10 are H/E and extra section at level 5 is taken for Negative serum control Level 2 is S100 Level 4, 6 is PanMelanoma cocktail (Biocare Medical) Level 7, 8, 9, are held for as unstained and filed away additional H/E or IHC We usually aim for about 200 microns in between levels. This is entirely dependent on the degree of adipose tissue that was included around the node and the thickness of the node in the paraffin block. When we started this protocol about 5 years ago, we would get 1 or 2 paraffin blocks for analysis. Nowadays we typically receive 3-6 blocks per case (sometimes 2 cases per week) and this has impacted our workload. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 From: Amy Farnan farn...@nehealth.com Subject: [Histonet] sentinel lymph node melanoma protocols To: histonet@lists.utsouthwestern.edu Message-ID: 4de60e2b.26ed.00d...@nehealth.com Content-Type: text/plain; charset=US-ASCII Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple HE sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 HE sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. -- Message: 7 Date: Wed, 1 Jun 2011 09:40:03 -0700 (PDT) From: Dawud AlYasa dawudh...@yahoo.com Subject: [Histonet] Bone Marrow HE Protocols To: histonet@lists.utsouthwestern.edu Message-ID: 759774.67432...@web34506.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hello Histonet! ? Does anyone have an HE automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks. ? Dawud -- Message: 8 Date: Wed, 1 Jun 2011 09:54:01 -0700 From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Subject: [Histonet] pic of tattooed skin To: HISTONET histonet@lists.utsouthwestern.edu Message-ID: 55dcef43-7dd7-4117-a62d-5790da604...@email.arizona.edu Content-Type: text/plain; charset=us-ascii Good Morning! Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar? Last year's camp was on liver, which was way easier! Thanks. Andi Grantham -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 91, Issue 1 *** Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IDH1 R132H IHC
Rich, The latest I have is from an order I placed for this product in 2010. It may have changed. My catalog number is DIA H09L US Rep Kitty Stein k.stein.dian...@gmail.com phone 786-343-2692 Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Message: 1 Date: Sun, 27 Mar 2011 12:52:48 -0400 From: Richard Cartun rcar...@harthosp.org Subject: [Histonet] IDH1 R132H IHC To: Histonet histonet@lists.utsouthwestern.edu Message-ID: 4d8f331f.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII Is anyone doing IHC for the IDH1 R132H point mutation for astrocytoma and oligodendroglioma in formalin-fixed, paraffin-embedded brain tissue using the H09 clone? If so, how do you get the antibody? Directly from Germany or is there someone here in the USA that carries it? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare Universal Negative for PIN4
I use this in the same way as Mark Tarango... Here's the part number NC498 L 800-799-7499 is the phone for Biocare or www.biocare.net They're great with tech support and can give prices and different sizes. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] if you could pick your own floor surface...
Hi Friends, What type of floor surface would you pick if you could design your own histology lab? I was walking through Costco the other night and noticed theirs is just concrete. Compensating for leg fatigue on the Assistants who have to stand, I'm thinking this type of surface might be easiest to keep clean. What do you think about just concrete? Would there need to be a paraffin scrape nightly? Would that be enough? If paraffin is tracked in the morning will this type of floor be slippy (Pittsburgh slang) by 3pm? What flooring do you have and what drawbacks and advantages do you see with it? Many thanks and no skids! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 * Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] positive charged slides
I've been vacillating about sharing my observations on this topic, mainly because I don't have a good answer for solving the problems that everyone is describing. I have had similar experiences. I have a validated slide that for me is the lesser of all evils. The slides that work the best for me, BUT STILL GIVE ME FITS are the super frost EXTENDED plus slides. Fisher number 22-034-979. These slides have an extended painted area. This allows for automated stainer labels to fit completely on the paint. I have found with the standard paint, that the labels extend over onto the glass and they just don't stick as well. These semi detached labels might or might not be factoring into the staining issues. So I rule that out. Also, I monitor the lot numbers. I have learned that these lot numbers can give the exact date of manufacture. The first numbers are the month, then day, then year. My boxes of slides also have the month and year of manufacture stamped right on the box.. My main problem with testing slides is that we go through lot numbers so quickly. If I order hordes of the same lot number, then are the slides that I open today as fresh as the slides I opened two months ago? I hate to have this attitude, but, then what's next, validating Kleenex we use, too? Do clinical labs have to validate every lot number of test tubes they use? The experts that manufacture these slides claim a specific expiration date. What criteria are they using to determine this date? Does the substance or charge on the slide deteriorate over a period of time, or all of a sudden on the expire date they just stop working? Ugh. I wonder if the scientists who put the chemistry onto these slides actually understand what the real world is going through. I have also observed different problems. Sometimes hydrophobic symptoms with patchy staining, sometimes tissue washing off for no explainable reason (ruled out poor processing). I use two different platforms, one where I immerse slides into a heated HIER bath and then lay flat on a rack for staining, and the other where the slides are placed onto the stainer and all steps are completed on the instrument. I can't pin point one thing these stainers could be doing that is contributing to the problem. The common item is the glass slide. Best I got! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I agree with Jose, etal.
Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is all over the board? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] biocare bcl 6
Message: 3 Date: Mon, 15 Jun 2009 15:34:33 -0400 From: Justin Peters jpet...@bostwicklaboratories.com Subject: [Histonet] BCL-6 To: histonet@lists.utsouthwestern.edu Message-ID: 24d22de9e488aa43bf92a4389f2ddb1f05dfa...@mail1.bostwick.com Content-Type: text/plain; charset=us-ascii I am having trouble getting bcl-6 antibody to work (Biocare CM223). Does anyone have a good protocol for me to try? Hi Justin, I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent. Most antibodies perform well with the green diluent; this particular antibody is best using the RED. I also use Mach4 detection... if problems persist, contact me offline, I may be able to help with some other suggestions. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
