[Histonet] RE: Histonet Digest, Vol 112, Issue 33
Hi Michael, I have always wrapped the cell blocks in lens paper. If it is a messy one I wrap it 2 or 3 times in lens paper. Helayne, Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pregnancy
Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to jascholefi...@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos) Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pregnancy (more)
Hi, Thanks all for all the info. We have a plan in place for the time being. She will only be embedding and sectioning slides at a station that we set up in the clinical lab. I will be doing all the rest of the stuff. Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Block File Cabinets
Hi All, Someone called me from a nearby Histo laboratory. They have approx 75 plastic block file cabinets that they would like to re-home for free, but new owner must pay shipping or come get them in Springfield, MO. If anyone is interested you can contact me at oreillepoint...@msn.com and I can put you in contact w/ the appropriate person to speak with. Have a lovely weekend, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor dehydration cycles
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between our Formalin and alcohols. 10% NBF, 10% NBF, PenFix, PenFix and then the alcohols starting at 95%. Speaking of 10% NBF has anyone ever used un-buffered formalin for routine processing? I heard of a place using un-buffered formalin and wondered if 1) would that be okay or is it harsh on tissue and 2) would it keep the salt deposits down? Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 111, Issue 7
Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? We use Clear-Rite 3 made by Richard Allan and is purchased at Cardinal. I do not think it is very expensive we buy in a case of 4 gallons. It does a decent job for clearing in the processor and de-paraffinizing slides, but we do coverslip out of xylene at the end of the stain line. Actually we never tried to coverslip out of it Clear-Rite 3 so can not honestly give you a critique in ref to that. We keep both on hand. Xylene for machine cleaning and coverslipping and Clear-Rite 3 for processing and de-paraffinizing. I like it better than Americlear and ProPar. http://www.cardinalhealth.com/us/en/distributedproducts/ASP/C4405-20.asp?cat=physician Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene/Paint Thinner
I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Haha- funny you should say that. I always swore that Mitaban (for canine demodex mange) was made out of xylene. Almost looks like watered down xylene. Well I checked the MSDS and it is but only the inactive ingredient part at a high %. On a lighter note that orange fragrance d-limonene flea dip is basically the same stuff as the Americlear is. Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bronch Order Forms
Hi All, Not sure how many of you do cytologies in your departments? We do quite a bit of bronchs around here and about 5 years ago a new physician who is no longer here developed the form that we currently use. It has never been liked by the nurses in scopes etc and it is pretty complicated. It allows Drs to put up to 5 microbiology, hematology, cyto or histo samples (many of which we share in all depts) on one form and has multiple check boxes to check as to what tests they want for each sample in multiple columns. We are looking to streamline this form to hopefully make it easier for everyone involved. Any of you have any Bronch ordering forms you really like or have used some in the past. Needs to be a paper order as we are not on a Lab system in Path at this point. Thanks, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Phoenix
Hi All, I will be looking for a great full-time position in the Phoenix area sometime in the future (4-6 mos). Any suggestions? Helayne Parker, H.T. (ASCP) CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Iron Stain
Hi All, Is there any specific reason why one iron stain kit I have recommends that you air-dry the bone marrow and blood smears after the Fe stain is completed and then coverslip them while other kits don't mention that at all. I have always stained my smears then dehydrate, clear and coverslip them like the tissue slides (at the end). Ergo Fe stain, rinse, paragon, rinse, 95% Alc, 100% Alc, xylene and coverslip. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hpar...@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slippery Floors due to paraffin
I was also taught years ago to make sure housekeeping does not put wax on your path lab floors. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hpar...@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFIP Closing
Sad that is where I went thru Histology Training at the Tri-Service School of Histotechnology in 1985. I was in a class of 10 Air Force guys and I was Navy and there was one other Navy guy. I was the only female in my class. It was interesting and have been doing Histology non-stop since then- wow 25+ years has gone by fast. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hpar...@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dictaphone System - HELP !!
Hi All, Does anybody use the old Dictaphones ? We have been using them and the company does not support them any longer. The one at our grossing station went down Friday and was replaced by a newer one (as we had purchased several from a hospital that was getting out of them). This one did not have a foot pedal so a foot pedal connection was adapted to it; however, when the other machine went down it was unplugged by another team member and all the programming was therefore lost. Does anyone have Dictaphones that are operated by means of a foot pedal who's IT team would be willing to share the proper method to program in a foot pedal to use w/ it. It is almost impossible to gross without a foot pedal. Thanks in advance. Sincerely, Helayne Parker, HT (ASCP) CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dictaphone Troubles are over
Thanks all- we got it fixed and it works like new now. Thanks, Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hpar...@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson Trichrome
We like the Sigma components. We buy them ala carte, though Poly Sci stuff is just the same. Helayne CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sorry about my last post
I did not mean to resend the whole digest back. I forget to erase. Helayne CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What New Tissue Processor Should we get ?
Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hpar...@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] High Profile Blades
I used to love the TBS blades (green- I think), but we use low profile here. We use Thermo and Accu Edge. Helayne Parker CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing for Prostate Biopsies
Can anyone tell me what/how prst bx are billed ?? Helayne Parker, HT (A.S.C.P.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
