[Histonet] RE: Water baths

[Pathology-Histology Sr. Supervisor]

 We have been checking ALL temps and recording every shift. These include 
cryostats, Embedding centers, Wax dispenser, Hot plates, Hot Ovens, Body 
Refrigerator, refrigerators, Water Baths, and room temps and Humidity%.

Muhammad Tahseen MLT (PMF), MT (JIMTEF) Japan
Senior supervisor histopathology
SKMCH&RC Lahore
Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Tuesday, December 02, 2014 8:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water baths

Does anyone take daily temperature checks of their water bath (used for cutting 
only) for CAP?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] RE: Specimen numbering systems

[Pathology-Histology Sr. Supervisor]

I also agree with Dawn. We shall be start from Jan 2015 as this way.

Muhammad Tahseen, MT (JIMTEF) Japan
Senior supervisor Histopathology
SKMCH&RC Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan
Sent: Tuesday, November 25, 2014 12:42 AM
To: 'hymclab'; 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

I agree with Dawn . Basically use a numeric for the Block and an Alpha for the 
specimen. This way a large resection specimen would receive an A and all blocks 
for said specimen would be labeled as A1,A2,A3...ect 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Monday, November 24, 2014 2:26 PM
To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems

We do it the same as Toni and from the CAP Uniform Labeling of slides and 
Blocks presentation I attended at NSH that is what CAP came up with for uniform 
labeling (S14-1234 A1, A2, A3, B1, B2, etc...).



Dawn Schneider, HT(ASCP)
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54568
715-356-8174




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, November 21, 2014 2:18 PM
To: 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We use a numeric for the accession number (S-14-1234), followed by alpha for 
the specimen(A, B, C), and within that have numeric for the number of blocks in 
each specimen (A1, A2, A3). We haven't ever gone to "Z" in our current LIS, and 
the ability to add unlimited blocks to each specimen makes it easy to deal with.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Friday, November 21, 2014 3:11 PM
To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We are currently using "Numeric" for the specimen (or part) and "Alpha" for the 
block.  I don't like it; we frequently have "1Z" blocks for large CA 
resections!  I would like to change this going forward.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Friday, November 21, 2014 2:03 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Specimen numbering systems

Can large facilities of more than 500 beds please let me know how they are 
numbering their Surgical specimens.  Alpha for the Specimens and numeric for 
the Block or Numeric for the Specimen and Alpha for the Block.

Thanks,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


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[Histonet] RE: Squamous cells staining on H&E and IHC

[Pathology-Histology Sr. Supervisor]

When the sections are cut and taken on the slide from water bath, slide must 
only holds on the frosted end of the slides don't touch other part of slide.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCH&RC
Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, October 29, 2014 12:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Squamous cells staining on H&E and IHC


Does anyone else have problems with what looks like squamous cells staining on 
your H&E's and IHC's?  I'm trying to figure out how to eliminate that problem 
in our lab...wear gloves while cutting?  Change out water bath several times 
during shifts? Any suggestions?  Thanks!

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[Histonet] RE: Double Labeling EM Blocks

[Pathology-Histology Sr. Supervisor]

We are labeling all Blocks and slides with two identifier as hospital policy.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCH&RC
Lahore
Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neelam Joshi
Sent: Thursday, October 23, 2014 5:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Double Labeling EM Blocks

Hello,

Does anyone has a procedure for double labeling EM Blocks with two identifier?

Please let me know as soon as possible.

Thanks you all!

Neelam Joshi,HT
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RE: [Histonet] Paraffin Used

[Pathology-Histology Sr. Supervisor]

We infiltrate and embed with Paraplast Plus Surgipath.
Regards,
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCH&RC
Lahore
Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Friday, October 24, 2014 3:53 AM
To: Cooper, Brian; WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Paraffin Used

We do the same here.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Thursday, October 23, 2014 4:37 PM
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Paraffin Used

Hi Bill, 

We infiltrate with Paraplast, and embed with Paraplast Extra.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, October 23, 2014 11:48 AM
To: histonet
Subject: [Histonet] Paraffin Used

What paraffin do you use in your lab? Do you use different type for processing 
and embedding? Looking to investigate the top used paraffins for a workshop I 
am putting together.

Sent from my iPhone
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[Histonet] RE: Working on Saturday

[Pathology-Histology Sr. Supervisor]

Dear Lorraine,
Our Histology departments work on Saturday.
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCH&RC
Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smallwood, 
Lorraine
Sent: Thursday, October 23, 2014 10:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Working on Saturday

We are a 450 bed hospital.  Due to our size and volume of work, our work week 
is Monday-Saturday. Because of healthcare reform, budget cuts and staffing 
shortage, we are faced with closing the department on Saturday (!!Histotechs 
are happy!!). One tech will continue to come in on Saturday to process any 
breast specimens received.   I am curious to find out what is the norm 
concerning working on Saturdays.  How many other Histology departments work on 
Saturday?  Thanks in advance for your opinions!

Lorraine Smallwood MScEd, PA(ASCP)
BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT)
Pathologist Assistant


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RE: [Histonet] Validation of IHC

[Pathology-Histology Sr. Supervisor]

Dear Jamal,
Please see 
Principles of Analytic Validation of Immunohistochemistry Assays
Published : Archives of Pathology and Laboratory Medicine
March 19, 2014
cap.org
Later on I will send you template of IHC Validation, which we are using in 
SKMCH&RC.
Regards
Muhammad Tahseen
Senior Supervisor Histopathology
SKMCH&RC
Lahore, Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamal
Sent: Saturday, July 19, 2014 3:42 PM
To: Histonet edu
Subject: [Histonet] Validation of IHC

Dear colleagues

We purchased Ventana GX from Rosh for IHC

I reviewed the resent CAP check list 2013 for validation of IHC but I got 
confused.

I am using ready to use prediluted antibodies from Ventana and I want to 
validate the antibodies before use according to CAP requirements.

Can I have the validation plane and procedure for IHC antibodies.

 

 

 

Best Regards,

 

 

Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832| 
 j.rowa...@alborglaboratories.com 

Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
 www.alborglaboratories.com

 

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[Histonet] positive control indoleamine 2-3 dioxygenase

[Pathology-Histology Sr. Supervisor]

Hi All,
We are going to optimize indoleamine 2-3 dioxygenase (IDO1) expression in 
breast cancer tissue by immunohistochemistry on formalin fixed paraffin 
sections.
I shall be thankful  if any one please let me know about the "positive control.
Regards
Muhammad Tahseen
Sr.Supervisor Histopathology
SKMCH&RC Lahore
Pakistan
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[Histonet] company for antibody HSV(cocktail) on paraffin section

[Pathology-Histology Sr. Supervisor]

Hi All
We are looking company for antibody HSV(cocktail) on paraffin section, kindly 
any comments.
Regards
Muhammad Tahseen
Histology supervisor
SKMCH&RC
Lahore

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[Histonet] Recommendations cryostat

[Pathology-Histology Sr. Supervisor]

Hi All,
We are going to buy cryostat so opinion required CM 1950  LEICA verses HM525 NX 
Thermo.
Regards
Muhammad Tahseen
SKMCH&RC




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[Histonet] fixed fecal smears for CAP parasitology survey 2014 p3

[Pathology-Histology Sr. Supervisor]

Hi All,
We have received two fixed fecal smears from microbiology section ( CAP 
parasitology survey 2014 p3,)for trichrome or iron-Hematoxylin staining 
procedures.
Would I adopted any kind of trichrome or iron Hematoxylin or some specific ?
Thank,
Muhammad Tahseen
SKMCH&RC
Lahore

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[Histonet] negative control for FLI-1

[Pathology-Histology Sr. Supervisor]

Dear All,
We are looking  negative control for FLI-1, as positive control  we are using 
(PNET) on paraffin section successfully.
Our pathologist gave us Wilm's tumor and  IDC for Negative control but it not 
work good.
Thanks
Muhammad Tahseen
Sr. Supervisor Histology
SKMCH&RC Lahore
Pakistan
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RE: [Histonet] Choosing a tissue processor

[Pathology-Histology Sr. Supervisor]

Dear Aminul Islam Khan,
Shandon  Citadel 2000 or Sakura is good for this work load. We are using 
Peloris Leica and Leica ASP 300, our workload are 900 to 1200 blocks /day.
Regards,
Muhammad Tahseen
Senior Supervisor Histopathology
SKMCH&RC
Lahore Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Md. Aminul 
Islam Khan
Sent: Thursday, February 20, 2014 2:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Choosing a tissue processor

Hi everyone,

Can you help us in choosing a tissue autoprocessor. At present we doing manual 
tissue processing and our work load is around 90 to 120 blocks per day which 
may increase gradually. These include tiny biopsies (e.g.
gastric, pleural etc) to large specimens.
Now we want to go for automation.
People here are very poor for which we give the service at a low cost. They 
come from rural areas and staying for extradays in the city is very expensive 
for them.
So we want to shorten the processing time as well.
Also the technical hands are very scarce (i.e. if anything goes wrong with the 
autoprocessor).
What would be the best technology for us? Microwave, linear, crousel or vacuum 
processor?

Thanks in advance and regards,

aikhan
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