Re: [Histonet] Leica bond
Wendy, the Leica Bond is a great machine. We've had it for several years now and it provides excellent and consistent results. Paula Bio-Path Medical Group Fountain Valley, CA -Original Message- From: Miller, Wendy [mailto:miller.we...@mhsil.com] Sent: Wednesday, July 08, 2015 6:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica bond Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS Question
Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica ASP6025 Processor
Hello, We're considering the Leica ASP6025 processor for our laboratory and I was hoping I can get pros and cons or any comments such as in performance, reagent money savings, ease of use, any comment from you. Thank you so much in advance for taking the time to give me your thoughts, Paula Lucas Lab Manager Bio-Path Medical Group California ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Study using Resin Embedding
Hello, We are doing a study on molar roots for a company in Orange County, California. The study is working with tissues embedded in paraffin blocks and they would like a study using resin embedded histology. We do not have the capability to do this study, so if your company is able to help them, and would like to contact them to find out more information, please let me know and I will connect you to the right person. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job Opportunity/Fountain Valley, Orange County, CA
Please no hiring staff services calling. We are in need of a part-time histotechnician who can work on Tuesdays and Saturdays, and then also to fill in during the week when needed. Hours start at 5 am. We work Tuesdays through Saturdays. Only experienced histotechs apply, which means previous experience in a lab work environment (not as an intern), and being able to cut (microtomy), and embed all types of tissues. We are a busy lab who supports hospitals, surgery centers and physician practice offices Please send resume to: Paula Lucas 714-755-2984. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing/PA needed, orange county ca
We need assistance with our volume. Please call if interested and if you have any questions. Please ask for Russell Blayney. Thank you, Paula Lucas Lab Manager Biopath Medical Group 714-433-1330 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New code 88343
Hello, Can someone shed some light for me on this new code for IHC? I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time Histotech Position/Orange County California
A full-time histotech position is opening at the end of August. The opening is a shift from 4:00am to 12:30pm, Tuesday through Saturdays. On-call duty 1 or 2 Saturdays per month is also required. Certification is required. Duties include: microtomy, embedding, manual special stains, IHC. Please email a resume if you're interested. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recycled chemical testing
Hello, Could anyone give me a contact where I can send my recycled alcohol and xylene for quality assessment? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening orange county
We have a part-time histotech position available and anyone who is interested, please send me your resume by fax or email. The work days are Tuesday through Saturday starting at 5 am. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 Fax: 714-755-2984 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening in Orange county California
A part-time histotech position is open in our histology laboratory. Tuesday through Saturday starting at 5 am. If anyone is interested, please either fax or email me your resume. Thank you, Paula Lucas Bio-Path Medical Group Fountain Valley, CA 92708 Fax: 714-755-2984 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech opening Orange County California
We have a part time histotech position available working from 5 am on Tuesday through Saturdays. We are located in Fountain Valley, California. Please provide me a summary of your histotech experience and please send me your resume. Thank you, Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Saturday Coverage Orange County California
We are in need of a per diem histotech who can work on Saturday mornings. We are located in Fountain Valley, California. Please send me an email if interested or fax resume to: 714 755-2984 Thank you, Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job Opening in Southern California
A part-time entry level histotech position is available in our laboratory. Please email or fax resume to: Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 plu...@biopath.org 714-755-2984 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] service on the Sukura Prisma
Hello, Does anyone know of an alternate vendor besides Sakura who can do the PM's and any necessary adjustments/fixes that come up on the Prisma? Sakura bills an arm and a leg for someone to come out. $175 dollars just for travel expenses is gouging to me, so if we can find someone else who can do the work at a cheaper rate, that would be great. Thanks for any info, Paula Lucas Lab Manager BP Labs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding Media
Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC platforms/Sales Representatives
Hello, We are inquiring about IHC staining platforms and we've already contacted the Leica sales rep for the Bond. I would also like to contact Ventana to get a quote on their system, and I've tried to get in contact with a sales rep in our area but I haven't had anyone returning my calls. If there is a Vantana rep reading this, would you please contact the sales representative for my area and let him/her know we are interested in a quote? We are located in Orange County (Fountain Valley), California. For those who have experience with these two platforms, would you mind taking the time to share with me your views and experiences about the two systems? Thanks so much, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 714.433.1330 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Needed
Histotech needed for vacation coverages and Saturday coverage. We are an independent laboratory located in Fountain Valley, California. If interested, I will give you more details. Thank you, Paula Lucas Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TTF-1/background staining
Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 Fixation Requirement Responses/Thanks
Your information and suggestions helped me formulate a plan, so thank you to those who took the time and responded to my inquiry yesterday. Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TTF-1/background staining
How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 Fixation Requirement
Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat Question Please
Hello all Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat? If so, what is your opinion/pros and cons? Thanks so much in advance, Paula Lab Manager BP Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip Film
Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Film Removal
Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Per Diem Histotech Needed (Orange County, California)
Hello- We are located in Fountain Valley, California and we are seeking a per diem histotech to cover during vacation times or other times when the techs are scheduled off. We are seeking a technician who has experience in embedding and cutting surgical tissue cases. Coverage START time would be early morning hours starting between 4:30 am to 7 am, preferably 5 am start time but I can be somewhat flexible with that. The total hours in a day would be between 4 and 6 hours. If interested, please give me a summary of your work history. Please no recruiters/head hunters respond to my email, only qualified techs who are interested. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
I second the Prisma with attached coverslipper. We have been using it now for the past 2 months or so. It's really a non-hassle, state of the art piece of equipment. Paula -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, March 02, 2010 12:50 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: RE: [Histonet] HE stainer/coverslippers We just purchased the sakura prisma and attached glass coverslipper. We just love them. We tried a lot of glass coverslippers and this by far is the best one that we have tried. We run both HE and specials on the prisma and have not had a problem with the glass coverslipper. We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] HE stainer/coverslippers Sakura René J. --- On Tue, 3/2/10, Gauch, Vicki gau...@mail.amc.edu wrote: From: Gauch, Vicki gau...@mail.amc.edu Subject: [Histonet] HE stainer/coverslippers To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Saturday Tech Needed Orange County California
Hello histotechs- please email me if you're interested in working a few hours on Saturday mornings. We are flexible, and have another Saturday tech that can fill in if you can not work on certain dates. I'll give you more info privately. I'm only seeking histotechs who have at least 2 years experience embedding and cutting surgical/biopsy cases in a hospital or private lab setting. Thanks, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GI, Uro, or Derm Path Lab Set Up
And just remember to those techs out there accepting jobs like these because they are offering salaries that are way above the salary range, that the investors will want their money back at some point. And, if and when the pod lab is suffering financially, there go the investors, then the business and there goes your job. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, February 04, 2010 4:02 PM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These labs and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from pod labs that have worked under horrible conditions. I am also aware of a pod lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are Cherry-picking the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860)
[Histonet] Saturday Tech Needed- Orange County California
Hello, Private lab seeking a histoch to work on Saturdays. Will be rotating with the other Saturday techs. Shift starts at 5 or 6 am. If interested, please send me an email. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond/Novocastra Reagents
Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Auto-IHC Staining Issues
Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cellient Cell Block System
Hello, Can anyone tell me their experiences with the Hologic's Cellient Cell Block System? I appreciate any thoughts and comments about the system you have. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
